Molecular diversity of arbuscular mycorrhizal fungi and patterns of host association over time and space in a tropical forest

We have used molecular techniques to investigate the diversity and distribution of the arbuscular mycorrhizal (AM) fungi colonizing tree seedling roots in the tropical forest on Barro Colorado Island (BCI), Republic of Panama. In the first year, we sampled newly emergent seedlings of the understory treelet Faramea occidentalis and the canopy emergent Tetragastris panamensis, from mixed seedling carpets at each of two sites. The following year we sampled surviving seedlings from these cohorts. The roots of 48 plants were analysed using AM fungal-specific primers to amplify and clone partial small subunit (SSU) ribosomal RNA gene sequences. Over 1300 clones were screened for random fragment length polymorphism (RFLP) variation and 7% of these were sequenced. Compared with AM fungal communities sampled from temperate habitats using the same method, the overall diversity was high, with a total of 30 AM fungal types identified. Seventeen of these types have not been recorded previously, with the remainder being similar to types reported from temperate habitats. The tropical mycorrhizal population showed significant spatial heterogeneity and nonrandom associations with the different hosts. Moreover there was a strong shift in the mycorrhizal communities over time. AM fungal types that were dominant in the newly germinated seedlings were almost entirely replaced by previously rare types in the surviving seedlings the following year. The high diversity and huge variation detected across time points, sites and hosts, implies that the AM fungal types are ecologically distinct and thus may have the potential to influence recruitment and host composition in tropical forests

Multiple solutions to a magnetic nonlinear Choquard equation

We consider the stationary nonlinear magnetic Choquard equation [(-\mathrm{i}\nabla+A(x))^{2}u+V(x)u=(\frac{1}{|x|^{\alpha}}\ast |u|^{p}) |u|^{p-2}u,\quad x\in\mathbb{R}^{N}%] where $A\$is a real valued vector potential, $V$ is a real valued scalar potential$,$ $N\geq3$, $\alpha\in(0,N)$ and $2-(\alpha/N) <p<(2N-\alpha)/(N-2)$. \ We assume that both $A$ and $V$ are compatible with the action of some group $G$ of linear isometries of $\mathbb{R}^{N}$. We establish the existence of multiple complex valued solutions to this equation which satisfy the symmetry condition $$u(gx)=\tau(g)u(x)\text{\ \ \ for all}g\in G,\text{}x\in\mathbb{R}^{N},$$ where $\tau:G\rightarrow\mathbb{S}^{1}$ is a given group homomorphism into the unit complex numbers.Comment: To appear on ZAM

Measurements of Anisotropy in the Cosmic Microwave Background Radiation at 0.5 Degree Angular Scales Near the Star Gamma Ursae Minoris

We present results from a four frequency observation of a 6 x 0.6 degree strip of the sky centered near the star Gamma Ursae Minoris during the fourth flight of the Millimeter-wave Anisotropy eXperiment (MAX). The observation was made with a 1.4 degree peak-to-peak sinusoidal chop in all bands. The FWHM beam sizes were 0.55 +/- 0.05 degrees at 3.5 cm-1 and 0.75 +/-0.05 degrees at 6, 9, and 14 cm-1. During this observation significant correlated structure was observed at 3.5, 6 and 9 cm-1 with amplitudes similar to those observed in the GUM region during the second and third flights of MAX. The frequency spectrum is consistent with CMB and inconsistent with thermal emission from interstellar dust. The extrapolated amplitudes of synchrotron and free-free emission are too small to account for the amplitude of the observed structure. If all of the structure is attributed to CMB anisotropy with a Gaussian autocorrelation function and a coherence angle of 25', then the most probable values of DeltaT/TCMB in the 3.5, 6, and 9 cm-1 bands are 4.3 (+2.7, -1.6) x 10-5, 2.8 (+4.3, -1.1) x 10-5, and 3.5 (+3.0, -1.6) x 10-5 (95% confidence upper and lower limits), respectively.Comment: 16 pages, postscrip

Measurements of Anisotropy in the Cosmic Microwave Background Radiation at Degree Angular Scales Near the Stars Sigma Hercules and Iota Draconis

We present results from two four-frequency observations centered near the stars Sigma Hercules and Iota Draconis during the fourth flight of the Millimeter-wave Anisotropy eXperiment (MAX). The observations were made of 6 x 0.6-degree strips of the sky with 1.4-degree peak to peak sinusoidal chop in all bands. The FWHM beam sizes were 0.55+/-0.05 degrees at 3.5 cm-1 and a 0.75+/-0.05 degrees at 6, 9, and 14 cm-1. Significant correlated structures were observed at 3.5, 6 and 9 cm-1. The spectra of these signals are inconsistent with thermal emission from known interstellar dust populations. The extrapolated amplitudes of synchrotron and free-free emission are too small to account for the amplitude of the observed structures. If the observed structures are attributed to CMB anisotropy with a Gaussian autocorrelation function and a coherence angle of 25', then the most probable values are DT/TCMB = (3.1 +1.7-1.3) x 10^-5 for the Sigma Hercules scan, and DT/TCMB = (3.3 +/- 1.1) x 10^-5 for the Iota Draconis scan (95% confidence upper and lower limits). Finally a comparison of all six MAX scans is presented.Comment: 13 pages, postscript file, 2 figure

Simplicity and predictability: a phenomenological study of psychological flow in transactional workers

Psychological flow is a positive experience achieved through a near-balance of task challenge and skill capability, creating a merging of awareness and action and leading to an intrinsically rewarding feeling. Flow has typically been documented in persons who participate in work and leisure activities where they can exercise a large degree of creativity and agency over their actions in pursuit of their goals. The objective of the present study is to explore the lived experiences of flow in workers in roles where creativity and agency are typically not expected. An interpretative phenomenological analysis approach was employed to attain this objective. Semi-structured interviews were conducted with 17 adults whose role is to perform transactional work, which by its nature affords less opportunity for creative execution. Common themes about participants’ flow experiences are documented. Two broad types of flow are described and a connection is made that the present study’s participants achieve one of those flow types while working. Participants’ feelings, preferences, and actions are mapped to the nine conventional dimensions of flow. Specific non-task work system factors are discussed relative to their influence on participants’ attainment of flow. Limitations of the present study and recommended future research are discussed

What explains the uneven take-up of ISO 14001 at the global level?: a panel-data analysis

Since its release in the mid-1990s, close to 37 000 facilities have been certified to ISO 14001, the international voluntary standard for environmental management systems. Yet, despite claims that the standard can be readily adapted to very different corporate and geographic settings, its take-up has been highly geographically variable. This paper contributes to a growing body of work concerned with explaining the uneven diffusion of ISO 14001 at the global level. Drawing from the existing theoretical and empirical literature we develop a series of hypotheses about how various economic, market, and regulatory factors influence the national count of ISO 14001 certifications. These hypotheses are then tested using econometric estimation techniques with data for a panel of 142 developed and developing countries. We find that per capita ISO 14001 counts are positively correlated with income per capita, stock of foreign direct investment, exports of goods and services to Europe and Japan, and pressure from civil society. Conversely, productivity and levels of state intervention are negatively correlated. The paper finishes by offering a number of recommendations to policymakers concerned with accelerating the diffusion of voluntary environmental standards

Optical binding of particles with or without the presence of a flat dielectric surface

Optical fields can induce forces between microscopic objects, thus giving rise to new structures of matter. We study theoretically these optical forces between two spheres, either isolated in water, or in presence of a flat dielectric surface. We observe different behavior in the binding force between particles at large and at small distances (in comparison with the wavelength) from each other. This is due to the great contribution of evanescent waves at short distances. We analyze how the optical binding depends of the size of the particles, the material composing them, the wavelength and, above all, on the polarization of the incident beam. We also show that depending on the polarization, the force between small particles at small distances changes its sign. Finally, the presence of a substrate surface is analyzed showing that it only slightly changes the magnitudes of the forces, but not their qualitative nature, except when one employs total internal reflection, case in which the particles are induced to move together along the surface.Comment: 8 pages, 9 figures, and 1 tabl

Microwave Spectroscopy

Contains research objectives and reports on four research projects.Signal Corps Contract DA36-039-sc-7489

Detection of circulating tumour DNA is associated with inferior outcomes in Ewing sarcoma and osteosarcoma: a report from the Children's Oncology Group.

BackgroundNew prognostic markers are needed to identify patients with Ewing sarcoma (EWS) and osteosarcoma unlikely to benefit from standard therapy. We describe the incidence and association with outcome of circulating tumour DNA (ctDNA) using next-generation sequencing (NGS) assays.MethodsA NGS hybrid capture assay and an ultra-low-pass whole-genome sequencing assay were used to detect ctDNA in banked plasma from patients with EWS and osteosarcoma, respectively. Patients were coded as positive or negative for ctDNA and tested for association with clinical features and outcome.ResultsThe analytic cohort included 94 patients with EWS (82% from initial diagnosis) and 72 patients with primary localised osteosarcoma (100% from initial diagnosis). ctDNA was detectable in 53% and 57% of newly diagnosed patients with EWS and osteosarcoma, respectively. Among patients with newly diagnosed localised EWS, detectable ctDNA was associated with inferior 3-year event-free survival (48.6% vs. 82.1%; p = 0.006) and overall survival (79.8% vs. 92.6%; p = 0.01). In both EWS and osteosarcoma, risk of event and death increased with ctDNA levels.ConclusionsNGS assays agnostic of primary tumour sequencing results detect ctDNA in half of the plasma samples from patients with newly diagnosed EWS and osteosarcoma. Detectable ctDNA is associated with inferior outcomes

Nucleation in Systems with Elastic Forces

Systems with long-range interactions when quenced into a metastable state near the pseudo-spinodal exhibit nucleation processes that are quite different from the classical nucleation seen near the coexistence curve. In systems with long-range elastic forces the description of the nucleation process can be quite subtle due to the presence of bulk/interface elastic compatibility constraints. We analyze the nucleation process in a simple 2d model with elastic forces and show that the nucleation process generates critical droplets with a different structure than the stable phase. This has implications for nucleation in many crystal-crystal transitions and the structure of the final state