Restaurant site selection

"Restaurant site selection is a complex decision often made without proper planning or sufficient information. It is generally held that the site selection process is more art than science and therefore difficult to quantify. The most important aspect of site selection is to assure that all factors that could possibly have any bearing on the decision are considered carefully."--First paragraph.Neil P. Quirk (Management Assistance Officer, Small Business Administration ; St. Louis), Robert F. Lukowski and Dante M. Laudadio (State Extension Specialists, Food Service and Lodging Management ; University of Missouri--Columbia)Includes bibliographical references

Expansion, in vivo–ex vivo cycling, and genetic manipulation of primary human hepatocytes

Primary human hepatocytes (PHHs) are an essential tool for modeling drug metabolism and liver disease. However, variable plating efficiencies, short lifespan in culture, and resistance to genetic manipulation have limited their use. Here, we show that the pyrrolizidine alkaloid retrorsine improves PHH repopulation of chimeric mice on average 10-fold and rescues the ability of even poorly plateable donor hepatocytes to provide cells for subsequent ex vivo cultures. These mouse-passaged (mp) PHH cultures overcome the marked donor-to-donor variability of cryopreserved PHH and remain functional for months as demonstrated by metabolic assays and infection with hepatitis B virus and Plasmodium falciparum. mpPHH can be efficiently genetically modified in culture, mobilized, and then recultured as spheroids or retransplanted to create highly humanized mice that carry a genetically altered hepatocyte graft. Together, these advances provide flexible tools for the study of human liver disease and evaluation of hepatocyte-targeted gene therapy approaches