Chrysin-Loaded Microemulsion: Formulation Design, Evaluation and Antihyperalgesic Activity in Mice

Chrysin is a bioactive flavonoid found in pollens, passion flowers, honey, royal jelly, and propolis, which is commonly used as an ingredient in natural food supplements and is primarily re-sponsible for their pharmacological properties. A transparent chrysin-loaded microemulsion (CS-ME) prepared through a ternary phase diagram was evaluated for use as an antihyperalgesic formulation. It was formulated with 40% Labrasol\uae (surfactant), 5% isopropyl myristate (oil phase) and 55% water (aqueous phase) and classified as an oil-in-water (O/W) microsized system (74.4 \ub1 15.8 nm). Its negative Zeta potential ( 1216.1 \ub1 1.9 mV) was confirmed by polarized light microscopy and dynamic light scattering analysis. In vitro studies in Franz-type static diffusion cells showed that chrysin release from CS-ME followed zero-order kinetics. Oral administration of CS-ME in mice resulted in a statistically significantly reduction (p < 0.05) in carrageenan-induced mechanical hyperalgesia compared to the control group. Treatment with CS-ME also showed anti-inflammatory activity by significantly decreasing the TNF-\u3b1 level (p < 0.01) and increasing that of IL-10 (p < 0.05) compared to the control group. These results suggest that the proposed microsystem is a promising vector for the release of chrysin, being able to improve its capacity to modulate inflammatory and nociceptive responses

Feasibility Studies for Single Transverse-Spin Asymmetry Measurements at a Fixed-Target Experiment Using the LHC Proton and Lead Beams (AFTER@LHC)

The measurement of Single Transverse-Spin Asymmetries, A_N, for various quarkonium states and Drell–Yan lepton pairs can shed light on the orbital angular momentum of quarks and gluons, a fundamental ingredient of the proton-spin puzzle. The AFTER@LHC proposal combines a unique kinematic coverage and large luminosities thanks to the Large Hadron Collider beams to deliver precise measurements, complementary to the knowledge provided by collider experiments such as at RHIC. In this paper, we report on sensitivity studies for J/ ψ, Υ and Drell–Yan A_N done using the performance of LHCb-like or ALICE-like detectors, combined with polarised gaseous hydrogen and helium-3 targets. In particular, such analyses will provide us with new insights and knowledge about transverse-momentum-dependent parton distribution functions for quarks and gluons and on twist-3 collinear matrix elements in the proton and the neutron

The application of ecologically intensive principles to the systemic redesign of livestock farms on native grasslands: A case of co-innovation in Rocha, Uruguay

CONTEXT: Family-run cow-calf farms based on native grasslands exhibit low economic and social sustainability, as reflected in low family incomes and high workloads. Experimental results have shown that pasture–herd interaction management could improve native grasslands and animal productivity OBJECTIVE: This paper analyzes the extent to which the sustainability of family-run livestock farms based on native grasslands could be enhanced by a systemic redesign informed by ecological intensification practices. The research questions address the initial state of farm sustainability, key bottlenecks to improving farm sustainability, and changes in sustainability criteria achieved over three years of farm redesign. METHODS: The study was executed as part of a multi-level co-innovation project in Uruguay in which a team of scientist-practitioners and seven farm families participated in farm characterization, diagnosis, and redesign. The farm characterization took the form of indicators to describe the farms’ management and bio-physical subsystems. Redesign plans were negotiated between the research team and the farmers. Frequent monitoring and evaluation cycles enabled finetuning across the years of implementation. RESULTS AND CONCLUSION: Improvements were observed in the economic indicators gross margin (+55%), return to labor (+71%), and family income (+53%) and in the social indicator workload ( 22%), and the environmental indicators bird diversity and ecosystem integrity index were maintained or increased slightly. These changes were explained by the uptake of coherent sets of ecological intensification practices causing changes in forage height (+30%), forage allowance (+69%), pregnancy (+22), weight of weaning calf per mating cow (+32%), and presence of tussocks (+65%). Ecological intensification principles resulted in synergistic positive effects between productivity–biodiversity tradeoffs and the scope for enhanced farm resilience and stability. SIGNIFICANCE: Cow-calf family-run farms can be transformed to produce positive environmental and social effects and viable economic results. The implementation of projects in a co-innovation context may be taken as a guide to scaling up and scaling out the ecological intensification of livestock production on native grasslands, contributing to an extension system at the national level with the aim of improving cow-calf systems sustainability.Estación Experimental Agropecuaria BarilocheFil: Ruggia, Andrea. Instituto Nacional de Investigacion Agropecuaria (INIA). Programa Nacional de Investigacion en Produccion Familiar. Estacion Experimental INIA Las Brujas; UruguayFil: Dogliotti, Santiago. Universidad de la República. Facultad de Agronomía. Departamento de Produccion Vegetal; UruguayFil: Aguerre, Maria Veronica. Instituto Nacional de Investigacion Agropecuaria (INIA). Programa Nacional de Investigacion en Produccion Familiar. Estacion Experimental INIA Las Brujas; UruguayFil: Albicette, Maria Marta. Instituto Nacional de Investigacion Agropecuaria (INIA). Programa Nacional de Investigacion en Produccion Familiar. Estacion Experimental INIA Las Brujas; UruguayFil: Blumetto, Oscar. Instituto Nacional de Investigacion Agropecuaria (INIA). Programa Nacional de Investigacion en Produccion Familiar. Estacion Experimental INIA Las Brujas; UruguayFil: Cardozo, Geronimo. Instituto Nacional de Investigacion Agropecuaria (INIA). Programa Nacional de Investigacion en Pasturas y Forrajes. Estacion Experimental INIA Treinta y Tres; UruguayFil: Leoni, Carolina. Instituto Nacional de Investigacion Agropecuaria (INIA). Programa Nacional de Investigacion en Produccion Familiar. Estacion Experimental INIA Las Brujas; UruguayFil: Quintans, Graciela. Instituto Nacional de Investigacion Agropecuaria (INIA). Programa Nacional de Investigacion en Carne y Lana. Estacion Experimental INIA Treinta y Tres; UruguayFil: Scarlato, Santiago. nstituto Nacional de Investigacion Agropecuaria (INIA). Programa Nacional de Investigacion en Produccion Familiar. Estacion Experimental INIA Las Brujas; UruguayFil: Tittonell, Pablo Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche; ArgentinaFil: Tittonell, Pablo Adrian. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; ArgentinaFil: Rossing, Walter A.H. Wageningen University and Research. Farming Systems Ecology, Plant Sciences Group; Holand

HPLC-DAD analysis, antinociceptive and anti-inflammatory properties of the ethanolic extract of Hyptis umbrosa in mice

Hyptis umbrosa (syn. Mesosphaerum sidifolium) (Lamiaceae Family) has been used to treat several conditions such as gastrointestinal disorders, skin infections, nasal congestion, fever and cramps. The objective of this study was to evaluate the chemical composition, analgesic and anti-inflammatory profiles of ethanol extract from leaves of Hyptis umbrosa (EEB). HPLC-DAD was used to determine the fingerprint chromatogram of the extract. Male Swiss mice were orally pretreated with EEB (100, 200 or 400 mg/kg; 60 min before initiating algesic stimulation) and antinociceptive activity was assessed using the acetic acid-induced writhing model, formalin test and hyperalgesia induced by glutamate or capsaicin. Also, peritonitis was induced by the intrathoracic injection of carrageenan to quantify the total number of leukocytes. The presence of phenolic compounds in the extract was confirmed using HPLC-DAD. The treatment with EEB, at all doses, produced a significant analgesic effect against acetic acid-induced antinociceptive activity. In the formalin test, only the 400-mg/kg-dose of EEB had a significant effect in the first phase. However, all doses tested were able to reverse nociception in the second phase. The effect of all doses of EEB also showed a significant antinociceptive effect in the glutamate and capsaicin tests and inhibited the carrageenan-induced leukocyte migration to the peritoneal cavity. The present study suggests that the EEB possesses peripheral analgesic action and showed potential in reducing the spreading of the inflammatory processes. Also, it seems to be related with vanilloid and glutamate receptors

Antinociceptive effect of the ethanolic extract of Amburana cearensis (Allemão) A.C. Sm., Fabaceae, in rodents.

Efeito antinociceptivo do extrato etanólico de Amburana cearensis (Allemão) A.C. Sm., Fabaceae, em roedores

Study of the transverse mass spectra of strange particles in Pb-Pb collisions at 158 A GeV/c

The NA57 experiment has collected high statistics, high purity samples of \PKzS and \PgL, $\Xi$ and $\Omega$ hyperons produced in Pb-Pb collisions at 158 $A$ GeV/$c$. In this paper we present a study of the transverse mass spectra of these particles for a sample of events corresponding to the most central 53% of the inelastic Pb-Pb cross-section. We analyse the transverse mass distributions in the framework of the blast-wave model for the full sample and, for the first time at the SPS, as a function of the event centrality.Comment: 22 pages, 14 figures, submitted to J. Phys. G: Nucl. Phy

J/psi azimuthal anisotropy relative to the reaction plane in Pb-Pb collisions at 158 GeV per nucleon

The J/$\psi$ azimuthal distribution relative to the reaction plane has been measured by the NA50 experiment in Pb-Pb collisions at 158 GeV/nucleon. Various physical mechanisms related to charmonium dissociation in the medium created in the heavy ion collision are expected to introduce an anisotropy in the azimuthal distribution of the observed J/$\psi$ mesons at SPS energies. Hence, the measurement of J/$\psi$ elliptic anisotropy, quantified by the Fourier coefficient v$_2$ of the J/$\psi$ azimuthal distribution relative to the reaction plane, is an important tool to constrain theoretical models aimed at explaining the anomalous J/$\psi$ suppression observed in Pb-Pb collisions. We present the measured J/$\psi$ yields in different bins of azimuthal angle relative to the reaction plane, as well as the resulting values of the Fourier coefficient v$_{2}$ as a function of the collision centrality and of the J/$\psi$ transverse momentum. The reaction plane has been estimated from the azimuthal distribution of the neutral transverse energy detected in an electromagnetic calorimeter. The analysis has been performed on a data sample of about 100 000 events, distributed in five centrality or p$_{\rm T}$ sub-samples. The extracted v$_{2}$ values are significantly larger than zero for non-central collisions and are seen to increase with p$_{\rm T}$.Comment: proceedings of HP08 conference corrected a typo in one equatio