Drosophila melanogaster mutant tan

Drosophila melanogaster gene tan was originally discovered in the early 20th century as a mutant strain lacking the dark pi gment pattern of wild-type (wt) f lies and, therefore, showing a light yellowish brown color (McEwen, 1918). Flies lack ing Tan function also exhibited abnormalities in vision (Benzer, 1967; Inoue et al. , 1988; True et al. , 2005), and tan males displayed an abnormal courtship behavior (Cook, 1980; Tomkins et al. , 1982). tan 1 ( t 1 ) and tan 3 ( t 3 ) alleles were found as spontaneous mutations, t 3 mutant being apparently lighter than t 1 (Brehme, 1941). tan is the structural gene for N- β -alanyldopamine hydrolase (NBAD-hydrolase or Tan protein), the enzyme that generates dopamine (DA) from NBAD (Wright, 1987; True et al. , 2005). Tan is expressed as a precursor protein of 43.7 kDa. Th is precursor is clea ved into two subunits of 29.9 and 13.8 kDa that apparently conform together a he terodimeric active protein (Wagner et al. , 2007). The enzyme that generates NBAD from DA, th e opposite reaction to the one catalyzed by Tan, is the NBAD-synthase or E bony protein (Wright, 1987; Pérez et al ., 1997), which is codified by the gene ebony . Since both Tan and Ebony ar e involved in cuticle tanni ng, carcinine re gulation, and NBAD metabolism in nervous tissue (Wright, 1987; Pérez et al. , 1997, 2004; Hovemann et al. , 1998; Borycz et al. , 2002; True et al. , 2005), it has been suggested that they function together in a system regulating the levels of dopamine during cuticle sclerotization a nd histamine in the visual metabolism (Borycz et al. , 2002; Pérez et al. , 2010). During the last few years, several publicati ons appeared regarding NBAD-synthase (Wappner et al ., 1996a, b; Pérez et al ., 1997, 2002, 2004, 2010; Hovemann et al. , 1998; Borycz et al. , 2002; Wittkopp et al., 2002; Schachter et al ., 2007), but very little is known about tan (True et al. , 2005; Wagner et al. , 2007). Thus, it was important to furthe r characterize the NBAD-hydrolase in D. melanogaster wt and in mutants t 1 and t 3.Fil: Badaracco, Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Quesada Allue, Luis Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Pérez, Martín Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentin

Technical efficiency of dairy farms in Uruguay : a stochastic production frontier analysis

The productivity of Uruguayan dairy farms has been consistently growing for the last 40 years. This process has implied the adoption of new technologies which have had significant effects on the production system. The efficiency with which available technologies are used influence output growth. Hence, assuring and enhancing dairy farms’ productivity and efficiency represent an important challenge to improve the competitiveness of the sector and achieve sustained economic growth. The overall objective of this study is to analyze the efficiency performance of dairy farms in Uruguay. Using a cross-sectional database, this study estimates a Cobb-Douglas stochastic production frontier and technical inefficiency model for dairy farms to determine the effect of each input on the production frontier and the principal factors that explain differences in farm efficiency. Results show that the number of milking cows has the highest effect on production, followed by the total consumption of feed, including concentrated feed, hay and silage. Although veterinary, agronomic and accounting assistance matter, the major determinants of efficiency differences are farmers’ specialization in dairy farming and the usage of artificial insemination. Overall, farm profiles indicate that those in the high efficiency group achieve a higher level of milk production than those less efficient; and they produce under a more intensive production system than farmers in low efficiency group

State of the Art of Geodiversity, Geoconservation, and Geotourism in Costa Rica

Over the last decades, Costa Rica became established as a world-leading ecotourism destination due to its environmental policies and environmental awareness. The country is located in a dynamic region where the combination of tectonics and volcanism, and tropical climate and vegetation have molded its landscapes. Our aim is to carry out a review of the geodiversity, geoconservation, and geotourism status in Costa Rica. We analyzed di erent geomorphic environments (volcanic, coastal, karstic, glacial, and fluvial), prospecting the importance of environmental policies and the Conservation Areas National System for the promotion of national geoheritage through geotourism. Our results are critical for the promotion of geosciences to the wider public throughout geotourism and conservation decision-makers. In dynamic, geomorphic, tropical, and developing countries with strong anthropic pressures over their geo- and biodiversity, geoheritage studies might be a priority for increasing their revenues through geotourism and reducing the pressure on their natural resources.UCR::Vicerrectoría de Docencia::Ciencias Sociales::Facultad de Ciencias Sociales::Escuela de Geografí

Tropical paleoglacial geoheritage inventory for geotourism management of Chirripó National Park, Costa Rica

Globally, most active tropical glacial landscapes are found at elevations above 4000 m. Nonetheless, the presence of paleoglacial landforms in low latitudes, especially those formed during the Last Glacial Maximum (LGM), persists in different tropical regions. Chirripó National Park, in central-south Costa Rica, is one of these particular examples. The glacial and periglacial landscape in the park are located over 3000 m, with landforms such as arêtes, glacial cirques, moraines, till deposits, and glacial lakes. We performed an integrated approach for the geoheritage inventory and geotourism management, crossing geomorphological and cultural information. A total of 14 geomorphosites were assessed and their management discussed. These geomorphosites achieved scientific scores between 0.5 (Valle Talari, Cerro Urán, and Cerro Terbi) and 0.88 (Cerro Chirripó and Los Crestones). The average scientific value of the geomorphosites is 0.75, since they are well preserved (0.75), rare (0.57) and representative (0.84) of the region’s geomorphology. They also play an important role in the geographical history (0.62), as well as the significant use and management characteristics (0.8). Chirripó National Park has key importance both naturally and culturally for Costa Rica. Its geomorphosites assessment is of critical value for the Costa Rican Conservation Areas System in order to promote improved geotourism. Geoheritage mapping can offer more opportunities of transferring geoscience knowledge to a larger public and policy makers. Our study aims to improve and further the state of the art of tropical glacial geoheritage.UCR::Vicerrectoría de Docencia::Ciencias Sociales::Facultad de Ciencias Sociales::Escuela de Geografí

Coarse-Grained Simulations of Release of Drugs Housed in Flexible Nanogels: New Insights into Kinetic Parameters

Funding: This research was funded by “Consejería de Economía, Conocimiento, Empresas y Universidad, Junta de Andalucía, Programa Operativo FEDER Andalucía 2014-2020”, grant number P20_00138 as well as “Ministerio de Ciencia e Innovación”, grant numbers PID2020-116615RA-I00 and PGC2018-098770-B-I00.The diffusion-controlled release of drugs housed in flexible nanogels has been simulated with the help of a coarse-grained model that explicitly considers polymer chains. In these in silico experiments, the effect of its flexibility is assessed by comparing it with data obtained for a rigid nanogel with the same volume fraction and topology. Our results show that the initial distribution of the drug can exert a great influence on the release kinetics. This work also reveals that certain surface phenomena driven by steric interactions can lead to apparently counterintuitive behaviors. Such phenomena are not usually included in many theoretical treatments used for the analysis of experimental release kinetics. Therefore, one should be very careful in drawing conclusions from these formalisms. In fact, our results suggest that the interpretation of drug release curves in terms of kinetic exponents (obtained from the Ritger–Peppas Equation) is a tricky question. However, such curves can provide a first estimate of the drug diffusion coefficient.Consejería de Economía, Conocimiento, Empresas y Universidad, Junta de Andalucía, Programa Operativo FEDER Andalucía 2014-2020, grant number P20_00138Ministerio de Ciencia e Innovación, grant numbers PID2020-116615RA-I00 and PGC2018-098770-B-I0

Georreferencias de la planimetría e imágenes digitales en Arqueología: Aplicación en la necrópolis de "La Cruz del Negro" (Carmona, Sevilla)

Partiendo de los estudios realizados en la necrópolis tartésica de la "Cruz del Negro" se expone la aplicación ala investigación arqueológica de los principios teóricos básicos para georreferenciar elementos espaciales e imágenes digitales. Se muestran las correcciones geométricas efectuadas sobre el registro planimétrico del lugar desde las primeras intervenciones hasta las últimas prospecciones magnéticas.From the studies accomplished in the "Cruz del Negro" tartesian burial site, the application to the archaeological research of the basic theoretical principles necessary for geo-referencing spatial elements and digital images is exposed. The geometric corrections made on the plannimetric record of the site from the first interventions up to the last magnetic surveys are shown

Efficient integral equation formulation for inductive waveguide components with posts touching the waveguide walls

In this paper a surface integral equation technique is employed for the analysis of inductive waveguide problems containing metallic or dielectric objects of arbitrary shape, focusing on the case where these objects are connected to the waveguide walls. Using the extinction theorem, the main problem is split into two problems. In the first one the parallel plate waveguide Green’s functions are used. Because of the choice of these functions, the side of the object touching the waveguide wall is not considered for discretization in a method of moments analysis. The second problem is applied inside the dielectric object, and uses the free space Green’s functions. It is shown that an additional spatial image is needed to impose the proper boundary conditions for the fields on the side touching the waveguide wall in the original problem. Results show the importance of including this additional image in the formulation for the correct behavior of the fields. With the proposed technique, the paper explores some alternatives for designing specific filter responses using dielectric posts inside cavity filters. Comparisons with a commercial finite elements tool demonstrate the accuracy of the proposed integral equation formulation.This work has been developed with support from the Spanish National Project (CICYT) with Ref TEC2004-04313-C02-02/TCM, and the Regional Seneca Project with Ref 02972/PI/05

Afectación ósea severa en reticulohistiosis multicéntrica, una enfermedad infrecuente: A propósito de un caso

La reticulohistiocitosis multicéntrica es una enfermedad muy infrecuente que afecta preferentemente a individuos de una edad media, y que se caracteriza por la aparición de nódulos diseminados en piel, mucosas, tejido subcutáneo y sinovial, xantomas, y afectación de pequeñas articulaciones de los dedos de las manos de apariencia similar a la artritis reumatoide, además de articulaciones de gran tamaño, principalmente hombros, caderas y rodillas, sobre las que sobreviene una artritis deformante y mutilante. Se presenta el caso de un varón de 55 años con afectación severa de hombros y caderas al que fue preciso recambiarle ambas articulaciones coxofemorales.Multicentric reticulohystiocytoses is a rare systemic disease which affects middle age patients, manifested by gradual development of nodules in the skin, mucosa, subcutaneous tissues and synovia, xantomas, and arthritis of the small joints of the hands, similar to reumathoid arthritis. Involvement of large joints as shoulders, hips and knee resulting in deforming and erosive arthritis. A case of a 55 year-old patient is presented, with severe destruction of shoulder and hips requiring bilateral total hip arthroplasty