71 research outputs found
EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA); Scientific Opinion on bovine lactoferrin:EFSA-Q-2010-01269
A new procedure for the synthesis of polyethylene glycol-protein adducts; Effects on function, receptor recognition, and clearance of superoxide dismutase, lactoferrin, and [alpha]2-macroglobulin
A new, simplified technique for the synthesis of polyethylene glycol (PEG) derivatives of proteins utilizing 1,1'-carbonyldiimidazole for PEG activation, is described. PEG derivatives of superoxide dismutase, [alpha]2-macroglobulin, [alpha]2-macroglobulin-trypsin, and lactoferrin were prepared and studied. Superoxide dismutase coupled to PEG preserved 95% of its original activity while its plasma half-life increased from 3.5 min to 9 or more hours depending on the PEG derivative studied. PEG-derivatized [alpha]2-macroglobulin showed decreased protease binding activity but PEG derivatives of preformed [alpha]2-macroglobulin-trypsin demonstrated no loss of activity. The plasma clearance of PEG-[alpha]2-macroglobulin-trypsin was prolonged significantly compared to native [alpha]2-macroglobulin-trypsin, particularly when a high-molecular-weight PEG was coupled to the protease inhibitor complex. The plasma clearance half-life of lactoferrin was increased 5-to 20-fold by this modification. Trinitrobenzenesulfonic acid titration studies demonstrated that [epsilon]-amino groups of lysine residues are modified by the coupling of carbonyldiimidazole-activated PEG to proteins.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/25222/1/0000663.pd
La secte, une nouvelle certitude sans limite
Querinjean Richard. La secte, une nouvelle certitude sans limite. In: Autres Temps. Cahiers d'éthique sociale et politique. N°72, 2001. pp. 31-41
Etude chromatographique et immunologique des dimères de serumalbumine bovine.
(Docteur en Sciences) -- Université catholique de Louvain, 196
Long-term Evolution of Teratomas Induced By Xenopus-borealis Embryos Incubated in a Mutagen and Implanted Into Sibling Toadlets
Optimalization of An Elisa for the Evaluation of Xenopus-laevis Vitellogenin and a Few Monoclonal-antibodies Against This Antigen
Allotypes of rat immunoglobulins—II. Distribution of the allotypes of kappa and alpha chain loci in different inbred strains of rats
- …