Mannose‐Modified Multi‐Walled Carbon Nanotubes as a Delivery Nanovector Optimizing the Antigen Presentation of Dendritic Cells

Dendritic cells (DCs) based cancer immunotherapy is largely dependent on adequate antigen delivery and efficient induction of DCs maturation to produce sufficient antigen presentation and ultimately lead to substantial activation of tumor‐specific CD8+ T cells. Carbon nanotubes (CNTs) have attracted great attention in biomedicine because of their unique physicochemical properties. In order to effectively deliver tumor antigens to DCs and trigger a strong anti‐tumor immune response, herein, a specific DCs target delivery system was assembled by using multi‐walled carbon nanotubes modified with mannose which can specifically bind to the mannose receptor on DCs membrane. Ovalbumin (OVA) as a model antigen, could be adsorbed on the surface of mannose modified multi‐walled carbon nanotubes (Man‐MWCNTs) with a large drug loading content. This nanotube‐antigen complex showed low cytotoxicity to DCs and was efficiently engulfed by DCs to induce DCs maturation and cytokine release in vitro, indicating that it could be a potent antigen‐adjuvant nanovector of efficient antigen delivery for therapeutic purpose.Perfectly delivered! Mannose‐modified multi‐walled carbon nanotubes (Man‐MWCNTs) could efficiently deliver a large amount of antigen to bone marrow derived dendritic cells (DCs) through ligand/receptor interactions of mannose, inducing enhanced BMDCs maturation and cytokines secretion.Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/150607/1/open201900126-sup-0001-misc_information.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/150607/2/open201900126.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/150607/3/open201900126_am.pd

Scaling-up Strategy as an Appropriate Approach for Sustainable New Town Development? Lessons from Wujin, Changzhou, China

China has achieved rapid urbanization and unprecedented economic booming over the past three decades. Numerous cities and towns dreamed of cloning the miracles of Shenzhen and Pudong, Shanghai, in terms of their international development. However, inappropriate development strategies have meant that the majority of fast expanding urban suburbs or newly developed towns suffer a high ratio of vacant dwellings in real estate markets and a massive loss of farmland. The frequent exposure of these empty cities to mass media or the public has urged urban governments to impose fiscal austerity. These unexpected and negative consequences of urban development have explicit conflicts with sustainability. This paper aims to provide a political economy view of these unsustainable outcomes of new development. To achieve this, the processes and agendas of new city or town planning in Wujin District, Changzhou City, are analyzed and evaluated from the perspective of scale theory. Extensive interviews conducted with local politicians at different levels, planners, real estate agents and local residents facilitate the interpretation of these processes and agendas. It is argued that the legends of Shenzhen and Pudong, Shanghai originate from a modified neoliberal capitalism intervention at the right time and place, with which other peer cities are not comparable. It is concluded that the scaling-up strategy is not appropriate for the local new town development of Wujin, which has led to unsustainable outcomes—empty cities and towns—and created important lessons for the sustainable development of Chinese cities

Socio-spatial differentiation and residential segregation in the Chinese city based on the 2000 community-level census data: A case study of the inner city of Nanjing

Socio-spatial differentiation and residential segregation have been studied extensively in numerous cities and have contributed significantly to the understanding of urban spatial and social structures. Analyses of diverse data sets at varied spatial scales have supported the development of theoretical frameworks. However, the majority of Chinese case studies published in recent decades were dominantly based on either non-spatial data or population census data at sub-district (or jiedao in Chinese) level. These analyses have been limited through using low-resolution aggregate data resulting in incomplete or biased findings. This paper aims to examine the fine-scale socio-spatial structure of the inner city of Nanjing using the fifth population census data of 2000 at the lowest spatial scale – community (or juweihui in Chinese) level. Our findings reveal that the policies of the socialist era and the initial outcomes of the introduction of a free market, particularly with regard to the creation of new elite spaces within the inner city, have shaped a complex pattern of socio-spatial differentiation and residential segregation

Traditional beliefs and practices in the postpartum period in Fujian Province, China: a qualitative study

<p>Abstract</p> <p>Background</p> <p><it>Zuo yuezi </it>is the month postpartum in China associated with a variety of traditional beliefs and practices. We explored the current status of <it>zuo yuezi </it>from social, cultural and western medical perspectives.</p> <p>Methods</p> <p>We interviewed family members (36) and health workers (8) in Fujian Province, selecting one rural and one rapidly developing urban county. We asked about their traditional beliefs and their behaviour postpartum. We used a framework approach to identify main themes. We categorised reported behaviour against their probable effects on health, drawing on Western standards.</p> <p>Results</p> <p>Respondents reported that <it>zuo yuezi </it>was commonly practiced in urban and rural families to help the mother regain her strength and protect her future health. <it>Zuo yuezi </it>included: dietary precautions, such as eating more food and avoiding cold food; behavioural precautions, such as staying inside the home, avoiding housework and limiting visitors; hygiene precautions, such as restricting bathing and dental hygiene; and practices associated with infant feeding, including supplementary feeding and giving honeysuckle herb to the infant. Respondents reported that the main reasons for adhering to these practices were respect for tradition, and following the advice of elders. Categorised against Western medical standards, several <it>zuo yuezi </it>practices are beneficial, including eating more, eating protein rich food, avoiding housework, and daily vulval and perineal hygiene. A few are potentially harmful, including giving honeysuckle herb, and avoiding dental hygiene. Some women reported giving infants supplementary feeds, although <it>zuo yuezi </it>emphasises breast feeding.</p> <p>Conclusion</p> <p><it>Zuo yuezi </it>is an important ritual in Fujian. In medical terms, most practices are beneficial, and could be used by health staff to promote health in this period. Further research on reported potentially harmful practices, such as supplements to breast feeding, is needed.</p

Challenges in QCD matter physics - The Compressed Baryonic Matter experiment at FAIR

Substantial experimental and theoretical efforts worldwide are devoted to explore the phase diagram of strongly interacting matter. At LHC and top RHIC energies, QCD matter is studied at very high temperatures and nearly vanishing net-baryon densities. There is evidence that a Quark-Gluon-Plasma (QGP) was created at experiments at RHIC and LHC. The transition from the QGP back to the hadron gas is found to be a smooth cross over. For larger net-baryon densities and lower temperatures, it is expected that the QCD phase diagram exhibits a rich structure, such as a first-order phase transition between hadronic and partonic matter which terminates in a critical point, or exotic phases like quarkyonic matter. The discovery of these landmarks would be a breakthrough in our understanding of the strong interaction and is therefore in the focus of various high-energy heavy-ion research programs. The Compressed Baryonic Matter (CBM) experiment at FAIR will play a unique role in the exploration of the QCD phase diagram in the region of high net-baryon densities, because it is designed to run at unprecedented interaction rates. High-rate operation is the key prerequisite for high-precision measurements of multi-differential observables and of rare diagnostic probes which are sensitive to the dense phase of the nuclear fireball. The goal of the CBM experiment at SIS100 (sqrt(s_NN) = 2.7 - 4.9 GeV) is to discover fundamental properties of QCD matter: the phase structure at large baryon-chemical potentials (mu_B > 500 MeV), effects of chiral symmetry, and the equation-of-state at high density as it is expected to occur in the core of neutron stars. In this article, we review the motivation for and the physics programme of CBM, including activities before the start of data taking in 2022, in the context of the worldwide efforts to explore high-density QCD matter.Comment: 15 pages, 11 figures. Published in European Physical Journal

Cis-eQTL analysis and functional validation of candidate susceptibility genes for high-grade serous ovarian cancer

Genome-wide association studies have reported 11 regions conferring risk of high-grade serous epithelial ovarian cancer (HGSOC). Expression quantitative trait locus (eQTL) analyses can identify candidate susceptibility genes at risk loci. Here we evaluate cis-eQTL associations at 47 regions associated with HGSOC risk (PPeer reviewe

A temporally cyclic growth model of urban spatial morphology in China: evidence from Kunming Metropolis

Rapid urbanization and complexity of political-economic transition in China has brought about continuous and remarkable changes of urban morphology over the past decades, which were driven by a mixture of spatial, social-economic and institutional forces. Understanding such urban morphological evolution requires new mixed evidences and holistic perspectives. In this paper, it is argued that two dominant types of urban growth in China: low-density expansion and high-density infill might be driven by different forces at different stages. To interpret the processes of urban development, two easy-to-understand morphological indicators: expansion-induced investment density index” (EID) and “infill-induced investment density index” (IID) are defined to measure the investment density per unit of developed land and used to compare the morphological changes between different phases in a long period by integrating spatial and socio-economic data. The temporal variation of these indicators suggests a cyclic growth model (CGM), which means the periodic switch between low density expansion and high-density infill. Using Kunming metropolis as a case study, this paper has confirmed that its urban morphological evolution from 1950-2014 was periodically and reciprocally driven by a set of vis-à-vis dualistic dynamics, in which low-density expansion is led by pro-growth infrastructure oriented public investment, while the high-density infill is activated by collective and rational actions of individual enterprises and their economic behaviors. It is concluded that the confirmed CGM model, together with two morphological indicators, offers a new holistic perspective and method to easily and integrally interpret urban morphological evolution and accordingly has potential theoretical implications for reasonably understanding the urbanisation in China

Space-Time Cascaded Processing-Based Adaptive Transient Interference Mitigation for Compact HFSWR

In high-frequency (HF) radar systems, transient interference is a common phenomenon that dramatically degrades the performance of target detection and remote sensing. Up until now, various suppression algorithms of transient interference have been proposed. They mainly concentrate on the skywave over-the-horizon radar on the basis of the assumption that the interference is sparse in a coherent processing interval (CPI). However, HF surface wave radar (HFSWR) often faces more complex transient interference due to various extreme types of weather, such as thunderstorm and typhoon, etc. The above algorithms usually suffer dramatic performance loss when transient interference contaminates the enormously continuous parts of a CPI. Especially for the compact HFSWR, which suffers from severe beam broadening and fewer array degrees of freedom. In order to solve the above problem, this study developed a two-dimensional interference suppression algorithm based on space-time cascaded processing. First, according to the spatial correlation of the compact array, the statistical samples of the main-lobe transient interference are estimated using a rotating spatial beam method. Next, an adaptive selection strategy is developed to obtain the optimal secondary samples based on information geometry distance. Finally, based on a quadratic constraint approximation, a precise estimation method of the optimal weight is developed when the interference covariance matrix is singular. The experimental results of simulation and measured data demonstrate that the proposed approach provides far superior suppression performance

Extended State Observer-Based Fuzzy Adaptive Backstepping Force Control of a Deep-Sea Hydraulic Manipulator with Long Transmission Pipelines

The force tracking control of deep-sea hydraulic manipulator systems with long transmission pipelines is disposed via fuzzy adaptive backstepping control based on an extended state observer in this paper. The pipeline model is established and then used to estimate the pressures in cylinder chambers, which are used to obtain the output force. In this process, the velocity of the piston, which is unmeasured, is needed, and an extended state observer is constructed to estimate the unmeasurable velocity signal. To cope with parameter uncertainties caused by changes in working depth, an adaptive algorithm is combined with the backstepping controller. Fuzzy logic is employed to design self-tuners that can automatically adjust the control parameters to guarantee force control performance from shallow seas to deep seas. The experimental results illustrate the success of the proposed control method. Comparative experimental results demonstrate that the extended state observer-based fuzzy adaptive backstepping controller has a relatively better tracking performance in different working conditions

Extended State Observer-Based Fuzzy Adaptive Backstepping Force Control of a Deep-Sea Hydraulic Manipulator with Long Transmission Pipelines

The force tracking control of deep-sea hydraulic manipulator systems with long transmission pipelines is disposed via fuzzy adaptive backstepping control based on an extended state observer in this paper. The pipeline model is established and then used to estimate the pressures in cylinder chambers, which are used to obtain the output force. In this process, the velocity of the piston, which is unmeasured, is needed, and an extended state observer is constructed to estimate the unmeasurable velocity signal. To cope with parameter uncertainties caused by changes in working depth, an adaptive algorithm is combined with the backstepping controller. Fuzzy logic is employed to design self-tuners that can automatically adjust the control parameters to guarantee force control performance from shallow seas to deep seas. The experimental results illustrate the success of the proposed control method. Comparative experimental results demonstrate that the extended state observer-based fuzzy adaptive backstepping controller has a relatively better tracking performance in different working conditions