Endothelial HO-1 induction by model TG-rich lipoproteins is regulated through a NOX4-Nrf2 pathway

Circulating levels of chylomicron remnants (CMRs) increase postprandially and their composition directly reflects dietary lipid intake. These TG-rich lipoproteins likely contribute to the development of endothelial dysfunction, albeit via unknown mechanisms. Here, we investigated how the FA composition of CMRs influences their actions on human aortic endothelial cells (HAECs) by comparing the effects of model CMRs—artificial TG-rich CMR-like particles (A-CRLPs)—containing TGs extracted from fish, DHA-rich algal, corn, or palm oils. HAECs responded with distinct transcriptional programs according to A-CRLP TG content and oxidation status, with genes involved in antioxidant defense and cytoprotection most prominently affected by n-3 PUFA-containing A-CRLPs. These particles were significantly more efficacious inducers of heme oxygenase-1 (HO-1) than n-6 PUFA corn or saturated FA-rich palm CRLPs. Mechanistically, HO-1 induction by all CRLPs requires NADPH oxidase 4, with PUFA-containing particles additionally dependent upon mitochondrial reactive oxygen species. Activation of both p38 MAPK and PPARβ/δ culminates in increased nuclear factor erythroid 2-related factor 2 (Nrf2) expression/nuclear translocation and HO-1 induction. These studies define new molecular pathways coupling endothelial cell activation by model CMRs with adaptive regulation of Nrf2-dependent HO-1 expression and may represent key mechanisms through which dietary FAs differentially impact progression of endothelial dysfunction

High-fat meals rich in EPA plus DHA compared with DHA only have differential effects on postprandial lipemia and plasma 8-isoprostane F-2 alpha concentrations relative to a control high-oleic acid meal: a randomized controlled trial

Background: Eicosapentaenoic acid (EPA) plus docosahexaenoic acid (DHA) supplementation has beneficial cardiovascular effects, but postprandial influences of these individual fatty acids are unclear. Objectives: The primary objective was to determine the vascular effects of EPA + DHA compared with DHA only during postprandial lipemia relative to control high–oleic acid meals; the secondary objective was to characterize the effects of linoleic acid–enriched high-fat meals relative to the control meal. Design: We conducted a randomized, controlled, double-blind crossover trial of 4 high-fat (75-g) meals containing 1) high–oleic acid sunflower oil (HOS; control), 2) HOS + fish oil (FO; 5 g EPA and DHA), 3) HOS + algal oil (AO; 5 g DHA), and 4) high–linoleic acid sunflower oil (HLS) in 16 healthy men (aged 35–70 y) with higher than optimal fasting triacylglycerol concentrations (mean ± SD triacylglycerol, 1.9 ± 0.5 mmol/L). Results: Elevations in triacylglycerol concentration relative to baseline were slightly reduced after FO and HLS compared with the HOS control (P < 0.05). The characteristic decrease from baseline in plasma nonesterified fatty acids after a mixed meal was inhibited after AO (Δ 0–3 h, P < 0.05). HLS increased the augmentation index compared with the other test meals (P < 0.05), although the digital volume pulse–reflection index was not significantly different. Plasma 8-isoprostane F(2α) analysis revealed opposing effects of FO (increased) and AO (reduced) compared with the control (P < 0.05). No differences in nitric oxide metabolites were observed. Conclusions: These data show differential postprandial 8-isoprostane F(2α) responses to high-fat meals containing EPA + DHA–rich fish oil compared with DHA-rich AO, but these differences were not associated with consistent effects on postprandial vascular function or lipemia. More detailed analyses of polyunsaturated fatty acid–derived lipid mediators are required to determine possible divergent functional effects of single meals rich in either DHA or EPA. This trial was registered at clinicaltrials.gov as NCT01618071

Broadband, radio spectro-polarimetric study of 100 radiative-mode and jet-mode AGN

We present the results from a broadband (1 to 3 GHz), spectro-polarimetry study of the integrated emission from 100 extragalactic radio sources with the ATCA, selected to be highly linearly polarized at 1.4 GHz. We use a general purpose, polarization model-fitting procedure that describes the Faraday rotation measure (RM) and intrinsic polarization structure of up to three distinct polarized emission regions or 'RM components' of a source. Overall, 37%/52%/11% of sources are best fit by one/two/three RM components. However, these fractions are dependent on the signal-to-noise ratio (S/N) in polarization (more RM components more likely at higher S/N). In general, our analysis shows that sources with high integrated degrees of polarization at 1.4 GHz have low Faraday depolarization, are typically dominated by a single RM component, have a steep spectral index, and a high intrinsic degree of polarization. After classifying our sample into radiative-mode and jet-mode AGN, we find no significant difference between the Faraday rotation or Faraday depolarization properties of jet-mode and radiative-mode AGN. However, there is a statistically significant difference in the intrinsic degree of polarization between the two types, with the jet-mode sources having more intrinsically ordered magnetic field structures than the radiative-mode sources. We also find a preferred perpendicular orientation of the intrinsic magnetic field structure of jet-mode AGN with respect to the jet direction, while no clear preference is found for the radiative-mode sources.Comment: 29 pages (including Appendix), 28 figures, 7 tables. Accepted for publication in MNRA

Revisiting the Hanbury Brown-Twiss set-up for fractional statistics

The Hanbury Brown-Twiss experiment has proved to be an effective means of probing statistics of particles. Here, in a set-up involving edge-state quasiparticles in a fractional quantum Hall system, we show that a variant of the experiment composed of two sources and two sinks can be used to unearth fractional statistics. We find a clear cut signature of the statistics in the equal-time current-current correlation function for quasiparticle currents emerging from the two sources and collected at the sinks.Comment: 4 pages, 3 figure

A radio-polarisation and rotation measure study of the Gum Nebula and its environment

The Gum Nebula is 36 degree wide shell-like emission nebula at a distance of only 450 pc. It has been hypothesised to be an old supernova remnant, fossil HII region, wind-blown bubble, or combination of multiple objects. Here we investigate the magneto-ionic properties of the nebula using data from recent surveys: radio-continuum data from the NRAO VLA and S-band Parkes All Sky Surveys, and H-alpha data from the Southern H-Alpha Sky Survey Atlas. We model the upper part of the nebula as a spherical shell of ionised gas expanding into the ambient medium. We perform a maximum-likelihood Markov chain Monte-Carlo fit to the NVSS rotation measure data, using the H-halpha data to constrain average electron density in the shell $n_e$. Assuming a latitudinal background gradient in RM we find $n_e=1.3^{+0.4}_{-0.4} {\rm cm}^{-3}$, angular radius $\phi_{\rm outer}=22.7^{+0.1}_{-0.1} {\rm deg}$, shell thickness $dr=18.5^{+1.5}_{-1.4} {\rm pc}$, ambient magnetic field strength $B_0=3.9^{+4.9}_{-2.2} \mu{\rm G}$ and warm gas filling factor $f=0.3^{+0.3}_{-0.1}$. We constrain the local, small-scale (~260 pc) pitch-angle of the ordered Galactic magnetic field to $+7^{\circ}\lesssim\wp\lesssim+44^{\circ}$, which represents a significant deviation from the median field orientation on kiloparsec scales (~-7.2$^{\circ}$). The moderate compression factor X=6.0\,^{+5.1}_{-2.5} at the edge of the H-alpha shell implies that the 'old supernova remnant' origin is unlikely. Our results support a model of the nebula as a HII region around a wind-blown bubble. Analysis of depolarisation in 2.3 GHz S-PASS data is consistent with this hypothesis and our best-fitting values agree well with previous studies of interstellar bubbles.Comment: 33 pages, 16 figures. Accepted by The Astrophysical Journa

Limits on a Variable Source of 511 keV Annihilation Radiation near the Galactic Center

The Gamma Ray Spectrometer (GRS) on the Solar Maximum Mission satellite (SMM) has observed a strong Galactic source of 511 keV annihilation radiation from its launch in 1980 to its reentry in 1989. These observations are consistent with an extended source having an intensity of about 0.002 gamma/sq cm/s averaged over the central radian of Galactic longitude. These data are searched for evidence of the variable Galactic center source of 511 keV line radiation which was reported to have reappeared in 1988 by Leventhal et al. The SMM data are consistent with, but do not require, a compact source emitting a time-averaged flux of about 0.0004 gamma/sq cm/s during about 3 month transits in 1987 and 1988; they are inconsistent with a compact source flux in excess of 0.0008 gamma/sq cm/s for each year

Balancing employee needs, project requirements and organisational priorities in team deployment

The 'people and performance' model asserts that performance is a sum of employee ability, motivation and opportunity (AMO). Despite extensive evidence of this people-performance link within manufacturing and many service sectors, studies within the construction industry are limited. Thus, a recent research project set out to explore the team deployment strategies of a large construction company with the view of establishing how a balance could be achieved between organisational strategic priorities, operational project requirements and individual employee needs and preferences. The findings suggested that project priorities often took precedence over the delivery of the strategic intentions of the organisation in meeting employees' individual needs. This approach is not sustainable in the long term because of the negative implications that such a policy had in relation to employee stress and staff turnover. It is suggested that a resourcing structure that takes into account the multiple facets of AMO may provide a more effective approach for balancing organisational strategic priorities, operational project requirements and individual employee needs and preferences more appropriately in the future

A multiple-scattering approach to interatomic interactions and superradiance in inhomogeneous dielectrics

The dynamics of a collection of resonant atoms embedded inside an inhomogeneous nondispersive and lossless dielectric is described with a dipole Hamiltonian that is based on a canonical quantization theory. The dielectric is described macroscopically by a position-dependent dielectric function and the atoms as microscopic harmonic oscillators. We identify and discuss the role of several types of Green tensors that describe the spatio-temporal propagation of field operators. After integrating out the atomic degrees of freedom, a multiple-scattering formalism emerges in which an exact Lippmann-Schwinger equation for the electric field operator plays a central role. The equation describes atoms as point sources and point scatterers for light. First, single-atom properties are calculated such as position-dependent spontaneous-emission rates as well as differential cross sections for elastic scattering and for resonance fluorescence. Secondly, multi-atom processes are studied. It is shown that the medium modifies both the resonant and the static parts of the dipole-dipole interactions. These interatomic interactions may cause the atoms to scatter and emit light cooperatively. Unlike in free space, differences in position-dependent emission rates and radiative line shifts influence cooperative decay in the dielectric. As a generic example, it is shown that near a partially reflecting plane there is a sharp transition from two-atom superradiance to single-atom emission as the atomic positions are varied.Comment: 18 pages, 4 figures, to appear in Physical Review

S-band Polarization All Sky Survey (S-PASS): survey description and maps

We present the S-Band Polarization All Sky Survey (S-PASS), a survey of polarized radio emission over the southern sky at Dec~$< -1^\circ$ taken with the Parkes radio telescope at 2.3~GHz. The main aim was to observe at a frequency high enough to avoid strong depolarization at intermediate Galactic latitudes (still present at 1.4 GHz) to study Galactic magnetism, but low enough to retain ample Signal-to-Noise ratio (S/N) at high latitudes for extragalactic and cosmological science. We developed a new scanning strategy based on long azimuth scans, and a corresponding map-making procedure to make recovery of the overall mean signal of Stokes $Q$ and $U$ possible, a long-standing problem with polarization observations. We describe the scanning strategy, map-making procedure, and validation tests. The overall mean signal is recovered with a precision better than 0.5\%. The maps have a mean sensitivity of 0.81 mK on beam--size scales and show clear polarized signals, typically to within a few degrees of the Galactic plane, with ample S/N everywhere (the typical signal in low emission regions is 13 mK, and 98.6\% of the pixels have S/N $> 3$). The largest depolarization areas are in the inner Galaxy, associated with the Sagittarius Arm. We have also computed a Rotation Measure map combining S-PASS with archival data from the WMAP and Planck experiments. A Stokes $I$ map has been generated, with a sensitivity limited to the confusion level of 9 mK.Comment: Accepted for publication on MNRAS. Maps are available for download at the website indicated in the manuscrip