Detection of Elevated Signaling Amino Acids in Human Diabetic Vitreous by Rapid Capillary Electrophoresis

Elevated glutamate is implicated in the pathology of PDR. The ability to rapidly assess the glutamate and amino acid content of vitreous provides a more complete picture of the chemical changes occurring at the diabetic retina and may lead to a better understanding of the pathology of PDR. Vitreous humor was collected following vitrectomies of patients with PDR and control conditions of macular hole or epiretinal membrane. A capillary electrophoresis method was developed to quantify glutamate and arginine. The analysis is relatively fast (<6 minutes) and utilizes a poly(ethylene)oxide and sodium dodecylsulfate run buffer. Both amino acid levels show significant increases in PDR patients versus controls and are comparable to other reports. The levels of vitreal glutamate vary inversely with the degree of observed hemorrhage. The results demonstrate a rapid method for assessment of a number of amino acids to characterize the chemical changes at the diabetic retina to better understand tissue changes and potentially identify new treatments

On the collisional disalignment of dust grains in illuminated and shaded regions of IC 63

Interstellar dust grain alignment causes polarization from UV to mm wavelengths, allowing the study of the geometry and strength of the magnetic field. Over the last couple of decades, observations and theory have led to the establishment of the radiative alignment torque mechanism as a leading candidate to explain the effect. With a quantitatively well constrained theory, polarization can be used not only to study the interstellar magnetic field, but also the dust and other environmental parameters. Photodissociation regions, with their intense, anisotropic radiation fields, consequent rapid H2 formation, and high spatial density-contrast provide a rich environment for such studies. Here we discuss an expanded optical, NIR, and mm-wave study of the IC 63 nebula, showing strong H2 formation-enhanced alignment and the first direct empirical evidence for disalignment due to gas-grain collisions using high-resolution HCO+(J = 1-0) observations. We find that a relative amount of polarization is marginally anticorrelated with column density of HCO+. However, separating the lines of sight of optical polarimetry into those behind, or in front of, a dense clump as seen from γ Cas, the distribution separates into two well defined sets, with data corresponding to "shaded" gas having a shallower slope. This is expected if the decrease in polarization is caused by collisions since collisional disalignment rate is proportional to RC∝nT" role="presentation">RC∝nT−−√ . Ratios of the best-fit slopes for the "illuminated" and "shaded" samples of lines of sight agrees, within the uncertainties, with the square root of the two-temperature H2 excitation in the nebula seen by Thi et al.Fil: Soam, Archana. Sofia Science Center; Estados UnidosFil: Anderson, B. G. Sofia Science Center; Estados UnidosFil: Acosta Pulido, Jose. Instituto de Astrofisica de Canarias; EspañaFil: Fernandez Lopez, Manuel. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Argentino de Radioastronomía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Argentino de Radioastronomía; ArgentinaFil: Vaillancourt, J. E.. Lincoln Laboratory; Estados UnidosFil: Widicus Weaver, S. L.. Department Of Chemistry; Estados UnidosFil: Piirola, V.. University Of Turku; FinlandiaFil: Gordon, M. S.. Sofia Science Center; Estados Unido

On the Collisional Disalignment of Dust Grains in Illuminated and Shaded Regions of IC 63

Interstellar dust grain alignment causes polarization from UV to mm wavelengths, allowing the study of the geometry and strength of the magnetic field. Over the last couple of decades, observations and theory have led to the establishment of the radiative alignment torque mechanism as a leading candidate to explain the effect. With a quantitatively well constrained theory, polarization can be used not only to study the interstellar magnetic field, but also the dust and other environmental parameters. Photodissociation regions, with their intense, anisotropic radiation fields, consequent rapid H-2 formation, and high spatial density-contrast provide a rich environment for such studies. Here we discuss an expanded optical, NIR, and mm-wave study of the IC 63 nebula, showing strong H-2 formation-enhanced alignment and the first direct empirical evidence for disalignment due to gas-grain collisions using high-resolution HCO+(J = 1-0) observations. We find that a relative amount of polarization is marginally anticorrelated with column density of HCO+. However, separating the lines of sight of optical polarimetry into those behind, or in front of, a dense clump as seen from gamma Cas, the distribution separates into two well defined sets, with data corresponding to "shaded" gas having a shallower slope. This is expected if the decrease in polarization is caused by collisions since collisional disalignment rate is proportional to R-C proportional to n root T. Ratios of the best-fit slopes for the "illuminated" and "shaded" samples of lines of sight agrees, within the uncertainties, with the square root of the two-temperature H-2 excitation in the nebula seen by Thi et al

Daily mean temperature and clinical kidney stone presentation in five U.S. metropolitan areas: a time-series analysis.

BACKGROUND: High ambient temperatures are a risk factor for nephrolithiasis, but the precise relationship between temperature and kidney stone presentation is unknown. OBJECTIVES: Our objective was to estimate associations between mean daily temperature and kidney stone presentation according to lag time and temperatures. METHODS: Using a time-series design and distributed lag nonlinear models, we estimated the relative risk (RR) of kidney stone presentation associated with mean daily temperatures, including cumulative RR for a 20-day period, and RR for individual daily lags through 20 days. Our analysis used data from the MarketScan Commercial Claims database for 60,433 patients who sought medical evaluation or treatment of kidney stones from 2005-2011 in the U.S. cities of Atlanta, Georgia; Chicago, Illinois; Dallas, Texas; Los Angeles, California; and Philadelphia, Pennsylvania. RESULTS: Associations between mean daily temperature and kidney stone presentation were not monotonic, and there was variation in the exposure-response curve shapes and the strength of associations at different temperatures. However, in most cases RRs increased for temperatures above the reference value of 10°C. The cumulative RR for a daily mean temperature of 30°C versus 10°C was 1.38 in Atlanta (95% CI: 1.07, 1.79), 1.37 in Chicago (95% CI: 1.07, 1.76), 1.36 in Dallas (95% CI: 1.10, 1.69), 1.11 in Los Angeles (95% CI: 0.73, 1.68), and 1.47 in Philadelphia (95% CI: 1.00, 2.17). Kidney stone presentations also were positively associated with temperatures < 2°C in Atlanta, and < 10°C in Chicago and Philadelphia. In four cities, the strongest association between kidney stone presentation and a daily mean temperature of 30°C versus 10°C was estimated for lags of ≤ 3 days. CONCLUSIONS: In general, kidney stone presentations increased with higher daily mean temperatures, with the strongest associations estimated for lags of only a few days. These findings further support an adverse effect of high temperatures on nephrolithiasis

Non-destructive Techniques Methodologies for the Detection of Ancient Structures under Heritage Buildings

[EN] Structures and elements buried beneath heritage buildings are frequent but are often unknown and inaccessible. Therefore, they are difficult to locate in general if an archaeological excavation is not carried out, with the economic cost and time involved. It is important to discover them in order to increase our knowledge of cultural heritage, as well as to know, recover and improve the state of conservation of the materials that make up these structures. This paper presents methodologies for locating old structures using a low-cost NDT approach, with a qualitative and quantitative analysis of GPR profiles in heritage buildings. Small perforations are performed at critical points and introducing an endoscope for verification. Various crypts have been located using the proposed methodologies in a real study case: The Church of the Asución of Llíria in Spain.Gil Benso, E.; Mas Tomas, MDLA.; Lerma Elvira, C.; Torner, ME.; Vercher Sanchis, J. (2021). Non-destructive Techniques Methodologies for the Detection of Ancient Structures under Heritage Buildings. International journal of architectural heritage (electronic). 15(10):1457-1473. https://doi.org/10.1080/15583058.2019.1700320S14571473151

2D-Tasks for Cognitive Rehabilitation

Neuropsychological Rehabilitation is a complex clinic process which tries to restore or compensate cognitive and behavioral disorders in people suffering from a central nervous system injury. Information and Communication Technologies (ICTs) in Biomedical Engineering play an essential role in this field, allowing improvement and expansion of present rehabilitation programs. This paper presents a set of cognitive rehabilitation 2D-Tasks for patients with Acquired Brain Injury (ABI). These tasks allow a high degree of personalization and individualization in therapies, based on the opportunities offered by new technologies

Patient-reported outcomes in a phase III, randomized study of sunitinib versus interferon-{alpha} as first-line systemic therapy for patients with metastatic renal cell carcinoma in a European population

BACKGROUND: The purpose of this study is to evaluate the impact on the health-related quality of life (HRQoL) of sunitinib versus interferon-alpha (IFN-alpha) treatment in patients with metastatic renal cell carcinoma (mRCC). PATIENTS AND METHODS: In all, 304 mRCC patients (European cohort) were randomized 1 : 1 to receive sunitinib (50 mg/day for 4 weeks, followed by 2 weeks off) or IFN-alpha (9 million units s.c. injection three times/week). The following questionnaires were completed (days 1 and 28 per cycle): Functional Assessment of Cancer Therapy-General (FACT-G), the FACT-Kidney Symptom Index and the EuroQol Group's EQ-5D self-report questionnaire (EQ-5D). Results correspond to an ongoing trial with progression-free survival time as primary end point, and patients were still being followed up. Data were analyzed using repeated measures mixed effects models (MEMs) that allow the inclusion of initial differences and uncompleted repeated measures, with the assumption of data missing at random. Six-cycle results were included. RESULTS: Results consistently showed that patients in sunitinib group experienced statistically significantly milder kidney-related symptoms, better cancer-specific HRQoL and general health status (in social utility scores) during the study period as measured by these patient-reported outcome end points. No statistical differences between groups were found on the FACT-G physical well-being subscale or the EQ-5D VAS values. CONCLUSIONS: Results from MEM showed the sunitinib's benefit on HRQoL compared with IFN-alpha

H alpha Star Formation Rates in Massive Galaxies at z ~ 1

We present a near-infrared spectroscopic study of a stellar mass selected sample of galaxies at z~1 utilising the LIRIS multi-object spectrograph on the WHT. We detect continuum, and the H alpha line for our sample, which is one of the better direct tracers of star formation in external galaxies. We spectroscopically measure the H alpha emission from 41 massive (M_{*}>10^{10.5} Msol) galaxies taken from the POWIR Survey with spectroscopic redshifts 0.4<z_{spec}<1.4. We correct our H alpha fluxes for dust extinction by using multi-wavelength data, and investigate SFR trends with mass and colour. We find a drop in the fraction of massive galaxies with M_{*}>10^{11} Msol which are detected in H alpha emission at z<0.9. We furthermore find that the fraction of galaxies with H alpha emission drops steadily and significantly with redder (U-B) colours at z~1, and that the SSFR drops with increasing (U-B) colour for galaxies at all masses. By investigating the SFR-mass relation we find that the SFR is roughly constant with mass, in possible contrast to previous work, and that the specific star formation rate (SSFR) is lower in the most massive galaxies. The scatter in the SFR vs. mass relationship is very small for those systems with ongoing star formation which suggests that star formation in the most massive galaxies at z~1 shuts off rather abruptly over <1 Gyr, without an obvious gradual decline. We furthermore investigate the SFR as a function of (U-B) colour divided into different mass bins, revealing a tracer of the epoch of transition from star forming to passive, as a form of star formation "downsizing". This suggests that the shut off of star formation occurs before the change in a galaxy's colour. We find that galaxy stellar mass is the primary driving mechanisms behind the star formation history for these galaxies and discuss several possible mechanisms for regulating this process.Comment: 20 pages, accepted for publication in MNRA

Non-Replication of Genome-Wide Based Associations between Common Variants in INSIG2 and PFKP and Obesity in Studies of 18,014 Danes

(rs17782313) as genetic risk factors. = 2,158) from Steno Diabetes Center. rs17782313 were observed. rs7566605 may influence the level of BMI in combination with the level of physical activity