Mesonia oceanica sp. Nov., isolated from oceans during the tara oceans expedition, with a preference for mesopelagic waters

Strain ISS653T, isolated from Atlantic seawater, is a yellow pigmented, non-motile, Gram-reaction-negative rod-shaped bac-terium, strictly aerobic and chemoorganotrophic, slightly halophilic (1-15% NaCl) and mesophilic (4-37 °C), oxidase-and catalase-positive and proteolytic. Its major cellular fatty acids are iso-C15:0, iso-C15:0 2-OH, and iso-C17:0 3-OH; the major identified phospholipid is phosphatidylethanolamine and the major respiratory quinone is MK6. Genome size is 4.28 Mbp and DNA G+C content is 34.9 mol%. 16S rRNA gene sequence similarity places the strain among members of the family Flavobacteriaceae, with the type strains of Mesonia phycicola (93.2%), Salegentibacter mishustinae (93.1%) and Mesonia mobilis (92.9%) as closest relatives. Average amino acid identity (AAI) and average nucleotide identity (ANI) indices show highest values with M. mobilis (81% AAI; 78.9% ANI), M. phycicola (76% AAI; 76.3% ANI), Mesonia maritima (72% AAI, 74.9% ANI), Mesonia hippocampi (64% AAI, 70.8% ANI) and Mesonia algae (68% AAI; 72.2% ANI). Phylogenomic analysis using the Up-to-date-Bacterial Core Gene set (UBCG) merges strain ISS653T in a clade with species of the genus Mesonia. We conclude that strain ISS653T represents a novel species of the genus Mesonia for which we propose the name Mesonia oceanica sp. nov., and strain ISS653T (=CECT 9532T=LMG 31236T) as the type strain. A second strain of the species, ISS1889 (=CECT 30008) was isolated from Pacific Ocean seawater. Data obtained throughout the Tara oceans expedition indicate that the species is more abundant in the mesopelagic dark ocean than in the photic layer and it is more frequent in the South Pacific, Indian and North Atlantic oceans

Drinking Water Library: nuevo recurso para identificar cepas bacterianas en aguas de consumo

De acuerdo con el RD 140/2003, el agua apta para el consumo humano no debe mostrar presencia de los indicadores bacterianos Escherichia coli, Clostridium perfringens y Enterococcus sp. Pero, aunque no se detecten estos indicadores, el agua del grifo no es estéril y puede tener cierta cantidad de microrganismos que muchas veces puede generar colonias en las placas de cultivo. Lejos de representar un peligro para la salud humana, si se dispone de un método rápido, sencillo, robusto y fiable para la identificación de estas colonias, se puede avanzar mucho en el conocimiento de la microbiología del agua potable. El proyecto Drinking Water Library ha permitido desarrollar la primera base de datos para MALDI-TOF MS específica para bacterias presentes en aguas de consumo

Tracking down carbon inputs underground from an arid zone Australian calcrete.

Freshwater ecosystems play a key role in shaping the global carbon cycle and maintaining the ecological balance that sustains biodiversity worldwide. Surficial water bodies are often interconnected with groundwater, forming a physical continuum, and their interaction has been reported as a crucial driver for organic matter (OM) inputs in groundwater systems. However, despite the growing concerns related to increasing anthropogenic pressure and effects of global change to groundwater environments, our understanding of the dynamics regulating subterranean carbon flows is still sparse. We traced carbon composition and transformations in an arid zone calcrete aquifer using a novel multidisciplinary approach that combined isotopic analyses of dissolved organic carbon (DOC) and inorganic carbon (DIC) (δ13CDOC, δ13CDIC, 14CDOC and 14CDIC) with fluorescence spectroscopy (Chromophoric Dissolved OM (CDOM) characterisation) and metabarcoding analyses (taxonomic and functional genomics on bacterial 16S rRNA). To compare dynamics linked to potential aquifer recharge processes, water samples were collected from two boreholes under contrasting rainfall: low rainfall ((LR), dry season) and high rainfall ((HR), wet season). Our isotopic results indicate limited changes and dominance of modern terrestrial carbon in the upper part (northeast) of the bore field, but correlation between HR and increased old and 13C-enriched DOC in the lower area (southwest). CDOM results show a shift from terrestrially to microbially derived compounds after rainfall in the same lower field bore, which was also sampled for microbial genetics. Functional genomic results showed increased genes coding for degradative pathways-dominated by those related to aromatic compound metabolisms-during HR. Our results indicate that rainfall leads to different responses in different parts of the bore field, with an increase in old carbon sources and microbial processing in the lower part of the field. We hypothesise that this may be due to increasing salinity, either due to mobilisation of Cl- from the soil, or infiltration from the downstream salt lake during HR. This study is the first to use a multi-technique assessment using stable and radioactive isotopes together with functional genomics to probe the principal organic biogeochemical pathways regulating an arid zone calcrete system. Further investigations involving extensive sampling from diverse groundwater ecosystems will allow better understanding of the microbiological pathways sustaining the ecological functioning of subterranean biota

Climate-driven range extension of Amphistegina (protista, foraminiferida) : models of current and predicted future ranges

© The Author(s), 2013. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in PLoS ONE 8 (2013): e54443, doi:10.1371/journal.pone.0054443.Species-range expansions are a predicted and realized consequence of global climate change. Climate warming and the poleward widening of the tropical belt have induced range shifts in a variety of marine and terrestrial species. Range expansions may have broad implications on native biota and ecosystem functioning as shifting species may perturb recipient communities. Larger symbiont-bearing foraminifera constitute ubiquitous and prominent components of shallow water ecosystems, and range shifts of these important protists are likely to trigger changes in ecosystem functioning. We have used historical and newly acquired occurrence records to compute current range shifts of Amphistegina spp., a larger symbiont-bearing foraminifera, along the eastern coastline of Africa and compare them to analogous range shifts currently observed in the Mediterranean Sea. The study provides new evidence that amphisteginid foraminifera are rapidly progressing southwestward, closely approaching Port Edward (South Africa) at 31°S. To project future species distributions, we applied a species distribution model (SDM) based on ecological niche constraints of current distribution ranges. Our model indicates that further warming is likely to cause a continued range extension, and predicts dispersal along nearly the entire southeastern coast of Africa. The average rates of amphisteginid range shift were computed between 8 and 2.7 km year−1, and are projected to lead to a total southward range expansion of 267 km, or 2.4° latitude, in the year 2100. Our results corroborate findings from the fossil record that some larger symbiont-bearing foraminifera cope well with rising water temperatures and are beneficiaries of global climate change.This work was supported by grants from the German Science Foundation (DFG; www.dfg.de) to ML and SL (LA 884/10-1, LA 884/5-1)

Una asociación florística de helechos-bennettitales en acumulaciones travertínicas del Titónico-Berriasiense (Formación Aguilar, Burgos-Palencia, España) y sus implicaciones paleoclimáticas y vegetacionales

[EN] This report describes a macrofloral fossil assemblage discovered in travertine deposits of the Tithonian-Berriasian Aguilar Formation (provinces of Palencia and Burgos, N Spain). The assemblage includes megaremains of a single species of Filicales (Cladophlebis denticulata) and of eleven taxa tentatively identified as Bennettitales (Otozamites mundae, Otozamites sp., Ptilophyllum pecten, Ptilophyllum pectiniformis, Ptilophyllum sp. cf. pectiniformis, Ptilophyllum acutifolium, Ptilophyllum sp., Pterophyllum cycadites, Pterophyllum sp. cf. thomasii, Zamites pumilio, and cf. Pseudocycas sp.). Specimens were preserved as impressions coated with a microbial film up to 5 mm thick made up of: bacteria and cyanobacteria. Based on the available sedimentological and palaeobotanical data, a dry-savannah vegetation and an arid climate were deduced. Late Jurassic macrofloras are globally scarce and have not previously been reported in Spain. Hence, the Aguilar Formation macroflora provides valuable information on the coeval phytogeography and palaeoclimate of southwestern Europe. Furthermore, the lack of floras observed so far in palaeotravertine deposits older than Pliocene in age makes this macroflora exceptional.[ES] Se describe por vez primera vez una macroflora fósil acumulada en facies travertínicas de edad Titónico-Berriasiense, correspondientes a la Formación Aguilar (provincias de Burgos y Palencia). Esta macroflora incluye una especie de Filicales (Cladophlebis denticulata) y once taxa atribuibles al Orden Bennettitales (Otozamites mundae, Otozamites sp., Ptilophyllum pecten, Ptilophyllum pectiniformis, Ptilophyllum sp. cf. pectiniformis, Ptilophyllum acutifolium, Ptilophyllum spp., Pterophyllum cycadites, Pterophyllum sp. cf. thomasi, Zamites pumilio, and cf. Pseudocycas sp.). Los ejemplares corresponden a impresiones de restos de hojas cubiertos por un velo microbiano bien preservado, de cerca de 5 mm, formado por bacterias y cianobacterias. La composición de la flora y los datos sedimentológicos permiten inferir una vegetación de sabana que se desarrollo bajo un clima árido. Las macrofloras del Jurásico Superior son escasas a escala mundial, no habiendo sido descrita ninguna en España. Por todo ello, la macroflora de la Formación Aguilar aporta una relevante información que puede contribuir a una mejor comprensión fitogeográfica y paleoclimática del suroeste de Europa en dicha edad. Además, la ausencia, a nivel global, de floras acumuladas en travertinos anteriores al Plioceno, permite considerar esta macroflora como excepcional..V. Pujalte acknowledges the support of grant CGL2005- 02770/BTE awarded by the Ministerio de Educación y Ciencia for the fieldwork needed for this study.Peer reviewe

Parvicella tangerina i Lysobacter luteus, dos nous bacteris aïllats d'una planta depuradora d'aigües residuals que processa aigua de mar

Investigadores de la UAB, de l'Institut de Ciències del Mar i de la Universitat de València han descrit els bacteris Parvicella tangerina i Lysobacter luteus, dos nous microorganismes trobats en una mostra de fang activat d'una planta depuradora d'aigües residuals d'Almeria. Tots dos bacteris presenten propietats interessants a nivell biotecnològic.Investigadoras de la UAB, el Instituto de Ciencias del Mar y la Universidad de Valencia han descrito las bacterias Parvicella tangerina y Lysobacter luteus, dos nuevos microorganismos encontrados en una muestra de fango activado de una planta depuradora de aguas residuales de Almería. Ambas bacterias presentan propiedades interesantes a nivel biotecnológico.Researchers from the UAB, the Institut de Ciències del Mar and the Universitat de València have described Parvicella tangerina and Lysobacter luteus, two new microorganisms found in a sample of activated sludge from a wastewater treatment plant in Almeria. Both bacteria have interesting biotechnological properties

The Palaeocene ‘‘top chron C27n’’ transient greenhouse episode: evidence from marine pelagic Atlantic and peri-Tethyan sections

The early Cenozoic, which is punctuated by several negative carbon isotope excursions (CIEs), was a time of climatic and oceanographic transition from ’Greenhouse’ to ’Icehouse’ conditions. The occurrence of a 0.5& CIE starting at the top of Chron C27n (TC27N) is reconfirmed with stable isotope data from Zumaia (Spain) and Bjala (Bulgaria) localities. Spectral analysis on respective carbonate ⁄magnetic susceptibility proxy records substantiates the orbital cyclostratigraphy allowing correlation to a high-resolution benthic foraminifera isotope record from ODP Pacific Site 1209, that indicates a coeval 2 C transient warming. The hyperthermal event lasts 200 ka, contrasting with other short-lived events from the Eocene, and displays a relatively rapid onset and a longer tailing back to pre-event values similar to the Palaeocene–Eocene Thermal Maximum (PETM), though lower in amplitude. That a causal trigger for the TC27N event may be the onset of volcanism in the North Atlantic Igneous Province (NAIP) can be inferred from a 200-m-thick lava pile erupted during C27n ⁄ C26r polarity transition in the E Greenland margin

High-resolution intra- and interbasinal correlation of the Danian–Selandian transition (Early Paleocene): The Bjala section (Bulgaria) and the Selandian GSSP at Zumaia (Spain)

The Danian–Selandian (D–S) boundary has been identified for the first time in the Black Sea coast at Bjala (Bulgaria) based on a new integrated bio-, magneto- and cyclostratigraphic study. Several correlation criteria as established for the basal Selandian GSSP from Zumaia (Basque Basin) are evaluated. Noteworthy, is the almost complete lack of calcareous nannoplankton species Braarudosphaera bigelowi in the Bulgarian sections, a sharp decrease of which was indicated as suitable criteria for defining the D–S boundary as it occurred both at Zumaia and in the classical locations of the North Sea basin. Conversely, the second evolutionary radiation of the calcareous nannofossil genus Fasciculithus together with the occurrence of Fasciculithus tympaniformis that define the NP4/NP5 zonal boundary seem to be reliable criteria to approximate the D–S boundary. In detail, however, the best approach is to integrate biostratigraphic data within a magnetostratigraphic and/or cyclostratigraphic framework. Refinements on the placement of chron C27n at Zumaia and robust bed-by-bed correlation between several Basque sections and Bjala indicates that the D–S boundary is located 30 precession cycles (~630 ky) above C27n. In addition to the precession-related marl–limestone couplets and 100-ky eccentricity bundles recognized in the studied sections, expression of the stable 405-ky long eccentricity allows direct tuning to the astronomical solutions. A correlation of the land-based sections with previously tuned data from ODP Site1262 from the Southern Atlantic is challenged. Our choice is consistent with original tuning at Zumaia but shifts one 100-ky cycle older previous tuning from Site 1262 along the interval above C27n. Under the preferred tuning scheme the D–S boundary can be given an age of 61.641± 0.040 Ma on the La04 orbital solution

High-resolution intra- and interbasinal correlation of the Danian–Selandian transition (Early Paleocene): The Bjala section (Bulgaria) and the Selandian GSSP at Zumaia (Spain)

The Danian–Selandian (D–S) boundary has been identified for the first time in the Black Sea coast at Bjala (Bulgaria) based on a new integrated bio-, magneto- and cyclostratigraphic study. Several correlation criteria as established for the basal Selandian GSSP from Zumaia (Basque Basin) are evaluated. Noteworthy, is the almost complete lack of calcareous nannoplankton species Braarudosphaera bigelowi in the Bulgarian sections, a sharp decrease of which was indicated as suitable criteria for defining the D–S boundary as it occurred both at Zumaia and in the classical locations of the North Sea basin. Conversely, the second evolutionary radiation of the calcareous nannofossil genus Fasciculithus together with the occurrence of Fasciculithus tympaniformis that define the NP4/NP5 zonal boundary seem to be reliable criteria to approximate the D–S boundary. In detail, however, the best approach is to integrate biostratigraphic data within a magnetostratigraphic and/or cyclostratigraphic framework. Refinements on the placement of chron C27n at Zumaia and robust bed-by-bed correlation between several Basque sections and Bjala indicates that the D–S boundary is located 30 precession cycles (~630 ky) above C27n. In addition to the precession-related marl–limestone couplets and 100-ky eccentricity bundles recognized in the studied sections, expression of the stable 405-ky long eccentricity allows direct tuning to the astronomical solutions. A correlation of the land-based sections with previously tuned data from ODP Site1262 from the Southern Atlantic is challenged. Our choice is consistent with original tuning at Zumaia but shifts one 100-ky cycle older previous tuning from Site 1262 along the interval above C27n. Under the preferred tuning scheme the D–S boundary can be given an age of 61.641± 0.040 Ma on the La04 orbital solution.Published511-5332.2. Laboratorio di paleomagnetismoJCR Journalrestricte