136 research outputs found

    Reconstruction of metabolic pathways for the cattle genome

    Get PDF
    <p>Abstract</p> <p>Background</p> <p>Metabolic reconstruction of microbial, plant and animal genomes is a necessary step toward understanding the evolutionary origins of metabolism and species-specific adaptive traits. The aims of this study were to reconstruct conserved metabolic pathways in the cattle genome and to identify metabolic pathways with missing genes and proteins. The MetaCyc database and PathwayTools software suite were chosen for this work because they are widely used and easy to implement.</p> <p>Results</p> <p>An amalgamated cattle genome database was created using the NCBI and Ensembl cattle genome databases (based on build 3.1) as data sources. PathwayTools was used to create a cattle-specific pathway genome database, which was followed by comprehensive manual curation for the reconstruction of metabolic pathways. The curated database, CattleCyc 1.0, consists of 217 metabolic pathways. A total of 64 mammalian-specific metabolic pathways were modified from the reference pathways in MetaCyc, and two pathways previously identified but missing from MetaCyc were added. Comparative analysis of metabolic pathways revealed the absence of mammalian genes for 22 metabolic enzymes whose activity was reported in the literature. We also identified six human metabolic protein-coding genes for which the cattle ortholog is missing from the sequence assembly.</p> <p>Conclusion</p> <p>CattleCyc is a powerful tool for understanding the biology of ruminants and other cetartiodactyl species. In addition, the approach used to develop CattleCyc provides a framework for the metabolic reconstruction of other newly sequenced mammalian genomes. It is clear that metabolic pathway analysis strongly reflects the quality of the underlying genome annotations. Thus, having well-annotated genomes from many mammalian species hosted in BioCyc will facilitate the comparative analysis of metabolic pathways among different species and a systems approach to comparative physiology.</p

    Genome sequencing and population genomic analyses provide insights into the adaptive landscape of silver birch

    Get PDF
    Silver birch (Betula pendula) is a pioneer boreal tree that can be induced to flower within 1 year. Its rapid life cycle, small (440-Mb) genome, and advanced germplasm resources make birch an attractive model for forest biotechnology. We assembled and chromosomally anchored the nuclear genome of an inbred B. pendula individual. Gene duplicates from the paleohexaploid event were enriched for transcriptional regulation, whereas tandem duplicates were overrepresented by environmental responses. Population resequencing of 80 individuals showed effective population size crashes at major points of climatic upheaval. Selective sweeps were enriched among polyploid duplicates encoding key developmental and physiological triggering functions, suggesting that local adaptation has tuned the timing of and cross-talk between fundamental plant processes. Variation around the tightly-linked light response genes PHYC and FRS10 correlated with latitude and longitude and temperature, and with precipitation for PHYC. Similar associations characterized the growth-promoting cytokinin response regulator ARR1, and the wood development genes KAK and MED5A.Peer reviewe

    Effect of photosynthetic photon flux density on growth, photosynthetic competence and antioxidant enzymes activity during ex vitro acclimatization of Dieffenbachia cultivars

    Get PDF
    The effects of 35, 70 and 100 µmol m−2 s−1 photosynthetic photon flux density (PPFD) were investigated on ex vitro acclimatization of micropropagated Dieffenbachia plants. Various growth characteristics, photosynthetic parameters and activities of antioxidant enzymes and dehydrins (DHN) were investigated. Fresh and dry plant biomass, plant height and root length were highest under the highest PPFD (100 µmol m−2 s−1), but this treatment was responsible for a reduction in the number of leaves. Chlorophyll and carotenoid contents and net photosynthesis were also optimal in plants grown under the highest irradiance. Stomatal resistance, transpiration rate and Fv/Fm values decreased with the incremental light irradiance. Activities of the antioxidant enzymes superoxide dismutase, catalase and glutathione peroxidase were higher in the plants treated with 70 and 100 µmol m−2 s−1 PPFD. Accumulation of 55 kDa, 40 and 22 kDa DHN was observed in all light treatments. These results depict that lower PPFD (35 µmol m−2 s−1) was suitable for acclimatization of Dieffenbachia plants. High PPFD (>70 µmol m−2 s−1) induced accumulation of antioxidants and accumulation of DHN in the plants which reveals enhanced stress levels

    Genome sequencing and population genomic analyses provide insights into the adaptive landscape of silver birch.

    Get PDF
    Silver birch (Betula pendula) is a pioneer boreal tree that can be induced to flower within 1 year. Its rapid life cycle, small (440-Mb) genome, and advanced germplasm resources make birch an attractive model for forest biotechnology. We assembled and chromosomally anchored the nuclear genome of an inbred B. pendula individual. Gene duplicates from the paleohexaploid event were enriched for transcriptional regulation, whereas tandem duplicates were overrepresented by environmental responses. Population resequencing of 80 individuals showed effective population size crashes at major points of climatic upheaval. Selective sweeps were enriched among polyploid duplicates encoding key developmental and physiological triggering functions, suggesting that local adaptation has tuned the timing of and cross-talk between fundamental plant processes. Variation around the tightly-linked light response genes PHYC and FRS10 correlated with latitude and longitude and temperature, and with precipitation for PHYC. Similar associations characterized the growth-promoting cytokinin response regulator ARR1, and the wood development genes KAK and MED5A

    Radioaktiivisten astioiden ja välineiden puhdistus

    No full text
    corecore