A circuit modeling technique for the ISO 7637-3 capacitive coupling clamp test

In this paper, we propose a transmission-line modeling technique for the ISO 7637-3 capacitive coupling clamp (CCC) test. Besides modeling the test bench, special attention is devoted to the CCC itself, for which an equivalent circuit is constructed based on the concept of surface transfer impedance and surface transfer admittance. The overall model is validated by means of measurements using a nonlinear circuit as device-under-test, as such demonstrating the appositeness to mimick the CCC test in simulations during the design phase

Harmonic balance surrogate-based immunity modeling of a nonlinear analog circuit

A novel harmonic balance surrogate-based technique to create fast and accurate behavioral models predicting, in the early design stage, the performance of nonlinear analog devices during immunity tests is presented. The obtained immunity model hides the real netlist, reduces the simulation time, and avoids expensive and time-consuming measurements after tape-out, while still providing high accuracy. The model can easily be integrated into a circuit simulator together with additional subcircuits, e.g., board and package models, as such allowing to efficiently reproduce complete immunity test setups during the early design stage and without disclosing any intellectual property. The novel method is validated by means of application to an industrial case study, being an automotive voltage regulator, clearly showing the technique's capabilities and practical advantages

Brexit, Rights and Devolution

This report records the final meeting and roundtable of the Brexit and Rights Engagement Network (BREN) on Friday 7 December 2018 at the Bingham Centre for the Rule of Law in London. Attendees at the roundtable included network members, fellow academics, representatives of the UK Government, the Northern Ireland Human Rights Commission, law firms, civil society, and NGOs. This roundtable followed two successful roundtables held at Edinburgh and Belfast earlier in the year. It combined the topics of both and brought all available network members together The purpose of the roundtable was to ignite debate amongst legal scholars and policy makers, and others working in a rights and devolution environment relating to interpretation, adjudication and enforcement of rights in the lead up to, and following “Brexit Day,” (29 March 2019). This report is split into four sections mirroring the programme of the day. Part A considers the adjudication of EU rights, but also their enforcement under the 2018 Act and the Withdrawal Agreement. Part B is dedicated to the same broad theme but focuses on ‘options for the postBrexit future’. Part C deals with possible development and opportunities as far as rights and devolution are concerned; and Part D shows how Brexit affects the devolution settlement. Most of the contributions to this and the other roundtables have been published as blog posts on the Europa Institute’s Europeanfutures blog. They can be found here: http://www.europeanfutures.ed.ac.uk/topics/bren Furthermore, there are audio recordings of the roundtable available, which can be found here: www.europa.ed.ac.uk/bren

Impact of Transplantation Timing on Renal Graft Survival Outcomes and Perioperative Complications

Nighttime organ transplantation aims to decrease cold ischemia duration, yet conflicting data exists on its impact on graft function and perioperative complications. This multicenter TRANSPLANT’AFUF study including 2,854 patients, transplanted between 1 January 2011, and 31 December 2022, investigated nighttime kidney transplantation’s impact (8:00 p.m.–8:00 a.m.) versus daytime (8:00 a.m.–8:00 p.m.) on surgical complications and graft survival. Overall, 2043 patients (71.6%) underwent daytime graft, while 811 (28.4%) underwent nighttime graft. No impact was observed of timing of graft surgery on graft survival with a median survival of 98 months and 132 months for daytime and nightime grafting, respectively (p = 0.1749). Moreover, no impact was observed on early surgical complications (Clavien I-II = 20.95% for DG and 20.10% for NG; Clavien III-IV-V = 15.42% for DG and 12.94% for NG; p = 0.0889) and late complications (>30 days) (Clavien I-II = 6.80% for DG and 5.67% for NG; Clavien III-IV-V = 12.78% for DG and 12.82% for NG; p = 0.2444). Noteworthy, we found a significant increase in Maastricht 3 donors’ rates in nighttime transplantation (5.53% DG vs. 21.45% NG; p < 0.0001). In conclusion, nighttime kidney transplantation did not impact early/late surgical complications nor graft survival

Functional Analysis of an Acid Adaptive DNA Adenine Methyltransferase from Helicobacter pylori 26695

HP0593 DNA-(N6-adenine)-methyltransferase (HP0593 MTase) is a member of a Type III restriction-modification system in Helicobacter pylori strain 26695. HP0593 MTase has been cloned, overexpressed and purified heterologously in Escherichia coli. The recognition sequence of the purified MTase was determined as 5′-GCAG-3′and the site of methylation was found to be adenine. The activity of HP0593 MTase was found to be optimal at pH 5.5. This is a unique property in context of natural adaptation of H. pylori in its acidic niche. Dot-blot assay using antibodies that react specifically with DNA containing m6A modification confirmed that HP0593 MTase is an adenine-specific MTase. HP0593 MTase occurred as both monomer and dimer in solution as determined by gel-filtration chromatography and chemical-crosslinking studies. The nonlinear dependence of methylation activity on enzyme concentration indicated that more than one molecule of enzyme was required for its activity. Analysis of initial velocity with AdoMet as a substrate showed that two molecules of AdoMet bind to HP0593 MTase, which is the first example in case of Type III MTases. Interestingly, metal ion cofactors such as Co2+, Mn2+, and also Mg2+ stimulated the HP0593 MTase activity. Preincubation and isotope partitioning analyses clearly indicated that HP0593 MTase-DNA complex is catalytically competent, and suggested that DNA binds to the MTase first followed by AdoMet. HP0593 MTase shows a distributive mechanism of methylation on DNA having more than one recognition site. Considering the occurrence of GCAG sequence in the potential promoter regions of physiologically important genes in H. pylori, our results provide impetus for exploring the role of this DNA MTase in the cellular processes of H. pylori