Off-axis retrieval of orbital angular momentum of light stored in cold atoms

We report on the storage of orbital angu- lar momentum (OAM) of light of a Laguerre-Gaussian mode in an ensemble of cold cesium atoms and its re- trieval along an axis different from the incident light beam. We employed a time-delayed four-wave mixing configuration to demonstrate that at small angle (2o), after storage, the retrieved beam carries the same OAM as the one encoded in the input beam. A calculation based on mode decomposition of the retrieved beam over the Laguerre-Gaussian basis is in agreement with the experimental observations done at small angle values. However, the calculation shows that the OAM retrieving would get lost at larger angles, reducing the fidelity of such storing-retrieving process. In addition, we have also observed that by applying an external magnetic field to the atomic ensemble the retrieved OAM presents Larmor oscillations, demonstrating the possibility of its manipulation and off-axis retrieval.Comment: 9 pages, 4 figure

Bose-Einstein condensation in dark power-law laser traps

We investigate theoretically an original route to achieve Bose-Einstein condensation using dark power-law laser traps. We propose to create such traps with two crossing blue-detuned Laguerre-Gaussian optical beams. Controlling their azimuthal order $\ell$ allows for the exploration of a multitude of power-law trapping situations in one, two and three dimensions, ranging from the usual harmonic trap to an almost square-well potential, in which a quasi-homogeneous Bose gas can be formed. The usual cigar-shaped and disk-shaped Bose-Einstein condensates obtained in a 1D or 2D harmonic trap take the generic form of a "finger" or of a "hockey puck" in such Laguerre-Gaussian traps. In addition, for a fixed atom number, higher transition temperatures are obtained in such configurations when compared with a harmonic trap of same volume. This effect, which results in a substantial acceleration of the condensation dynamics, requires a better but still reasonable focusing of the Laguerre-Gaussian beams

Diversidad genética de cepas españolas de Xanthomonas arboricola pv. pruni agente causal de la mancha bacteriana de los frutales de hueso y del almendro

PublishedTrabajo financiado por el Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, proyecto RTA2011-00140-d03-0

Polymorphisms of Pyrimidine Pathway Enzymes Encoding Genes and HLA-B*40∶01 Carriage in Stavudine-Associated Lipodystrophy in HIV-Infected Patients

Altres ajuts: Fundación para la Investigación y Prevención del SIDA en España (FIPSE 36610, 36572/06); Red de Investigación en SIDA (RIS RD12/0017/0005, RD12/0017/0014).To assess in a cohort of Caucasian patients exposed to stavudine (d4T) the association of polymorphisms in pyrimidine pathway enzymes and HLA-B*40∶01 carriage with HIV/Highly active antiretroviral therapy (HAART)-associated lipodystrophy syndrome (HALS). Three-hundred and thirty-six patients, 187 with HALS and 149 without HALS, and 72 uninfected subjects were recruited. The diagnosis of HALS was performed following the criteria of the Lipodystrophy Severity Grading Scale. Polymorphisms in the thymidylate synthase (TS) and methylene-tetrahydrofolate reductase (MTHFR) genes were determined by direct sequencing, HLA-B genotyping by PCR-SSOr Luminex Technology, and intracellular levels of stavudine triphosphate (d4T-TP) by a LC-MS/MS assay method. HALS was associated with the presence of a low expression TS genotype polymorphism (64.7% vs. 42.9%, OR = 2.43; 95%CI: 1.53-3.88, P<0.0001). MTHFR gene polymorphisms and HLA-B*40∶01 carriage were not associated with HALS or d4T-TP intracellular levels. Low and high expression TS polymorphisms had different d4T-TP intracellular levels (25.60 vs. 13.60 fmol/10 6 cells, P<0.0001). Independent factors associated with HALS were(OR [95%CI]: (a) Combined TS and MTHFR genotypes (p = 0.006, reference category (ref.): 'A+A'; OR for 'A+B' vs. ref.: 1.39 [0.69-2.80]; OR for 'B+A' vs. ref.: 2.16 [1.22-3.83]; OR for 'B+B' vs. ref.: 3.13, 95%CI: 1.54-6.35), (b) maximum viral load ≥5 log10 (OR: 2.55, 95%CI: 1.56-4.14, P = 0.001), (c) use of EFV (1.10 [1.00-1.21], P = 0.008, per year of use). HALS is associated with combined low-expression TS and MTHFR associated with high activity polymorphisms but not with HLA-B*40∶01 carriage in Caucasian patients with long-term exposure to stavudine

Genomics and transcriptomics of Xanthomonas campestris species challenge the concept of core type III effectome

The bacterial species Xanthomonas campestris infects a wide range of Brassicaceae. Specific pathovars of this species cause black rot (pv. campestris), bacterial blight of stock (pv. incanae) or bacterial leaf spot (pv. raphani). In this study, we extended the genomic coverage of the species by sequencing and annotating the genomes of strains from pathovar incanae (CFBP 1606R and CFBP 2527R), pathovar raphani (CFBP 5828R) and a pathovar formerly named barbareae (CFBP 5825R). While comparative analyses identified a large core ORFeome at the species level, the core type III effectome was limited to only three putative type III effectors (XopP, XopF1 and XopAL1). In Xanthomonas, these effector proteins are injected inside the plant cells by the type III secretion system and contribute collectively to virulence. A deep and strand-specific RNA sequencing strategy was adopted in order to experimentally refine genome annotation for strain CFBP 5828R. This approach also allowed the experimental definition of novel ORFs and non-coding RNA transcripts. Using a constitutively active allele of hrpG, a master regulator of the type III secretion system, a HrpG-dependent regulon of 141 genes co-regulated with the type III secretion system was identified. Importantly, all these genes but seven are positively regulated by HrpG and 56 of those encode components of the Hrp type III secretion system and putative effector proteins. This dataset is an important resource to mine for novel type III effector proteins as well as for bacterial genes which could contribute to pathogenicity of X. campestris