Ectopic positioning of the cell division plane is associated with single amino acid substitutions in the FtsZ-recruiting SsgB in Streptomyces

Microbial BiotechnologySolid state NMR/Biophysical Organic ChemistryMedical Biochemistr

Comprehensive Analysis of Binding Sites in Tubulin

Tubulin plays essential roles in vital cellular activities and is the target of a wide range of proteins and ligands. Here, using a combined computational and crystallographic fragment screening approach, we addressed the question of how many binding sites exist in tubulin. We identified 27 distinct sites, of which 11 have not been described previously, and analyzed their relationship to known tubulin\u2013protein and tubulin\u2013ligand interactions. We further observed an intricate pocket communication network and identified 56 chemically diverse fragments that bound to 10 distinct tubulin sites. Our results offer a unique structural basis for the development of novel small molecules for use as tubulin modulators in basic research applications or as drugs. Furthermore, our method lays down a framework that may help to discover new pockets in other pharmaceutically important targets and characterize them in terms of chemical tractability and allosteric modulation

PETrA: A Software-Based Tool for Estimating the Energy Profile of Android Applications

Energy efficiency is a vital characteristic of any mobile application, and indeed is becoming an important factor for user satisfaction. For this reason, in recent years several approaches and tools for measuring the energy consumption of mobile devices have been proposed. Hardware-based solutions are highly precise, but at the same time they require costly hardware toolkits. Model-based techniques require a possibly difficult calibration of the parameters needed to correctly create a model on a specific hardware device. Finally, software-based solutions are easier to use, but they are possibly less precise than hardware-based solution. In this demo, we present PETrA, a novel software-based tool for measuring the energy consumption of Android apps. With respect to other tools, PETrA is compatible with all the smartphones with Android 5.0 or higher, not requiring any device specific energy profile. We also provide evidence that our tool is able to perform similarly to hardware-based solutions.Software Engineerin

Software-Based Energy Profiling of Android Apps: Simple, Efficient and Reliable?

Modeling the power profile of mobile applications is a crucial activity to identify the causes behind energy leaks. To this aim, researchers have proposed hardware-based tools as well as model-based and software-based techniques to approximate the actual energy profile. However, all these solutions present their own advantages and disadvantages. Hardware-based tools are highly precise, but at the same time their use is bound to the acquisition of costly hardware components. Model-based tools require the calibration of parameters needed to correctly create a model on a specific hardware device. Software-based approaches do not need any hardware components, but they rely on battery measurements and, thus, they are hardware-assisted. These tools are cheaper and easier to use than hardware-based tools, but they are believed to be less precise. In this paper, we take a deeper look at the pros and cons of software-based solutions investigating to what extent their measurements depart from hardware-based solutions. To this aim, we propose a softwarebased tool named PETRA that we compare with the hardwarebased MONSOON toolkit on 54 Android apps. The results show that PETRA performs similarly to MONSOON despite not using any sophisticated hardware components. In fact, in all the apps the mean relative error with respect to MONSOON is lower than 0:05. Moreover, for 95% of the analyzed methods the estimation error is within 5% of the actual values measured using the hardware-based toolkit

Software-Based Energy Profiling of Android Apps: Simple, Efficient and Reliable?

Modeling the power profile of mobile applications is a crucial activity to identify the causes behind energy leaks. To this aim, researchers have proposed hardware-based tools as well as model-based and software-based techniques to approximate the actual energy profile. However, all these solutions present their own advantages and disadvantages. Hardware-based tools are highly precise, but at the same time their use is bound to the acquisition of costly hardware components. Model-based tools require the calibration of parameters needed to correctly create a model on a specific hardware device. Software-based approaches do not need any hardware components, but they rely on battery measurements and, thus, they are hardware-assisted. These tools are cheaper and easier to use than hardware-based tools, but they are believed to be less precise. In this paper, we take a deeper look at the pros and cons of software-based solutions investigating to what extent their measurements depart from hardware-based solutions. To this aim, we propose a softwarebased tool named PETRA that we compare with the hardwarebased MONSOON toolkit on 54 Android apps. The results show that PETRA performs similarly to MONSOON despite not using any sophisticated hardware components. In fact, in all the apps the mean relative error with respect to MONSOON is lower than 0:05. Moreover, for 95% of the analyzed methods the estimation error is within 5% of the actual values measured using the hardware-based toolkit.Software Engineerin

Data from: Oxidative stress is related to both melanin- and carotenoid-based ornaments in the common yellowthroat

Male ornaments are hypothesized to signal the ability of males to produce an effective immune response without extensive oxidative stress and damage to DNA. We examined this hypothesis in male common yellowthroats (Geothlypis trichas), which have two ornaments, a black (eumelanin-based) facial mask and a yellow (carotenoid-based) bib. In our study population, only the black mask is sexually selected. As predicted by the oxidative stress hypothesis, males with larger black masks were more resistant to oxidative stress, as measured by an in vitro assay of the resistance of erythrocytes to haemolysis by free radicals. Furthermore, males with larger masks also tended to have lower levels of glutathione, which was predicted because glutathione inhibits eumelanin production. In contrast, mask size was not related to absolute levels of oxidative stress measured in the plasma. Although the yellow bib is not under sexual selection in our population, males with larger bibs and feathers with greater carotenoid chroma had lower levels of oxidative stress. The oxidative stress hypothesis was first proposed for carotenoid-based ornaments. However, our results suggest that, even in the same individuals, carotenoid and eumelanin-based plumage ornaments may both signal the ability of males to resist or manage oxidative stress