The selectivity, voltage-dependence and acid sensitivity of the tandem pore potassium channel TASK-1 : contributions of the pore domains

We have investigated the contribution to ionic selectivity of residues in the selectivity filter and pore helices of the P1 and P2 domains in the acid sensitive potassium channel TASK-1. We used site directed mutagenesis and electrophysiological studies, assisted by structural models built through computational methods. We have measured selectivity in channels expressed in Xenopus oocytes, using voltage clamp to measure shifts in reversal potential and current amplitudes when Rb+ or Na+ replaced extracellular K+. Both P1 and P2 contribute to selectivity, and most mutations, including mutation of residues in the triplets GYG and GFG in P1 and P2, made channels nonselective. We interpret the effects of these—and of other mutations—in terms of the way the pore is likely to be stabilised structurally. We show also that residues in the outer pore mouth contribute to selectivity in TASK-1. Mutations resulting in loss of selectivity (e.g. I94S, G95A) were associated with slowing of the response of channels to depolarisation. More important physiologically, pH sensitivity is also lost or altered by such mutations. Mutations that retained selectivity (e.g. I94L, I94V) also retained their response to acidification. It is likely that responses both to voltage and pH changes involve gating at the selectivity filter

Observational properties of ultra-diffuse galaxies in low-density environments: field UDGs are predominantly blue and starforming

While we have learnt much about ultradiffuse galaxies (UDGs) in groups and clusters, relatively little is known about them in less dense environments. More isolated UDGs are important for our understanding of UDG formation scenarios because they form via secular mechanisms, allowing us to determine the relative importance of environmentally driven formation in groups and clusters. We have used the public Kilo-Degree Survey together with the Hyper Suprime-Cam Subaru Strategic Program to constrain the abundance and properties of UDGs in the field, targeting sources with low surface brightness (24.0 ≤ μ ¯ e,r μ¯e,r ≤ 26.5) and large apparent sizes (3.0 arcsec ≤ r ¯ e,r r¯e,r ≤ 8.0 arcsec). Accounting for several sources of interlopers in our selection based on canonical scaling relations, and using an empirical UDG model based on measurements from the literature, we show that a scenario in which cluster-like red-sequence UDGs occupy a significant number of field galaxies is unlikely, with most field UDGs being significantly bluer and showing signs of localized star formation. An immediate conclusion is that UDGs are much more efficiently quenched in high-density environments. We estimate an upper limit on the total field abundance of UDGs of 8 ± 3 × 10−3 cMpc−3 within our selection range. We also compare the total field abundance of UDGs to a measurement of the abundance of H i-rich UDGs from the literature, suggesting that they occupy at least one-fifth of the overall UDG population. The mass formation efficiency of UDGs implied by this upper limit is similar to what is measured in groups and clusters

The relationship between institutional marketing practice and international student choice

Mestrado em Ensino Superior (Erasmus Mundus)A presente tese discute o relacionamento entre o marketing institucional e a escolha do estudante internacional. A tese baseia-se num estudo de caso da Universidade de Monash, Austrália, e foram analisadas tanto a parte institucional como a parte internacional. Os resultados da investigação mostram que o marketing institucional pode ter uma relação com a escolha do estudante internacional e que as actividades de marketing podem, de alguma maneira, desempenhar um papel positivo no recrutamento de estudantes internacionais. Por fim, os resultados da investigação mostram que o marketing internacional pode ser uma forma útil de fornecer informação aos potenciais estudantes internacionais e ajudá-lo a tomar decisões. Dadas as amostras limitadas utilizadas no estudo, os resultados não podem ser generalizados. Assim, há a necessidade de estudar mais instituições de ensino superior. Investigação futura também pode averiguar se as actividades de marketing influenciam directamente o aumento do recrutamento de estudantes internacionais. ABSTRACT: This thesis discusses the relationship between institutional marketing and international student choice. The thesis is based on a case study of Monash University, Australia and both the institutional side and international side were analyzed. The research findings show that institutional marketing may have a relationship with international student choice and marketing activities in some extent play a role in positively influencing the international enrollment. Last but not least, the research findings show that institutional marketing can be a useful way to provide information to prospective international students and help them to make decisions. Due to the limited samples, the findings of the case study may not be generalized. Therefore, there is a need for more higher education institutions to be studied. Future research may also study whether the marketing activities have a direct influence on the increase of international student enrolment

MscS-like mechanosensitive channels in plants and microbes

The challenge of osmotic stress is something all living organisms must face as a result of environmental dynamics. Over the past three decades, innovative research and cooperation across disciplines have irrefutably established that cells utilize mechanically gated ion channels to release osmolytes and prevent cell lysis during hypoosmotic stress. Early electrophysiological analysis of the inner membrane of Escherichia coli identified the presence of three distinct mechanosensitive activities. The subsequent discoveries of the genes responsible for two of these activities, the mechanosensitive channels of large (MscL) and small (MscS) conductance, led to the identification of two diverse families of mechanosensitive channels. The latter of these two families, the MscS family, consists of members from bacteria, archaea, fungi, and plants. Genetic and electrophysiological analysis of these family members has provided insight into how organisms use mechanosensitive channels for osmotic regulation in response to changing environmental and developmental circumstances. Furthermore, determining the crystal structure of E. coli MscS and several homologues in several conformational states has contributed to our understanding of the gating mechanisms of these channels. Here we summarize our current knowledge of MscS homologues from all three domains of life and address their structure, proposed physiological functions, electrophysiological behaviors, and topological diversity

Preparing for low surface brightness science with the Vera C. Rubin Observatory:Characterization of tidal features from mock images

Tidal features in the outskirts of galaxies yield unique information about their past interactions and are a key prediction of the hierarchical structure formation paradigm. The Vera C. Rubin Observatory is poised to deliver deep observations for potentially millions of objects with visible tidal features, but the inference of galaxy interaction histories from such features is not straightforward. Utilizing automated techniques and human visual classification in conjunction with realistic mock images produced using the NewHorizon cosmological simulation, we investigate the nature, frequency, and visibility of tidal features and debris across a range of environments and stellar masses. In our simulated sample, around 80 per cent of the flux in the tidal features around Milky Way or greater mass galaxies is detected at the 10-yr depth of the Legacy Survey of Space and Time (30-31 mag arcsec-2), falling to 60 per cent assuming a shallower final depth of 29.5 mag arcsec-2. The fraction of total flux found in tidal features increases towards higher masses, rising to 10 per cent for the most massive objects in our sample (M* ∼1011.5 M⊙). When observed at sufficient depth, such objects frequently exhibit many distinct tidal features with complex shapes. The interpretation and characterization of such features varies significantly with image depth and object orientation, introducing significant biases in their classification. Assuming the data reduction pipeline is properly optimized, we expect the Rubin Observatory to be capable of recovering much of the flux found in the outskirts of Milky Way mass galaxies, even at intermediate redshifts (z < 0.2)

Identification of Intracellular and Plasma Membrane Calcium Channel Homologues in Pathogenic Parasites

Ca2+ channels regulate many crucial processes within cells and their abnormal activity can be damaging to cell survival, suggesting that they might represent attractive therapeutic targets in pathogenic organisms. Parasitic diseases such as malaria, leishmaniasis, trypanosomiasis and schistosomiasis are responsible for millions of deaths each year worldwide. The genomes of many pathogenic parasites have recently been sequenced, opening the way for rational design of targeted therapies. We analyzed genomes of pathogenic protozoan parasites as well as the genome of Schistosoma mansoni, and show the existence within them of genes encoding homologues of mammalian intracellular Ca2+ release channels: inositol 1,4,5-trisphosphate receptors (IP3Rs), ryanodine receptors (RyRs), two-pore Ca2+ channels (TPCs) and intracellular transient receptor potential (Trp) channels. The genomes of Trypanosoma, Leishmania and S. mansoni parasites encode IP3R/RyR and Trp channel homologues, and that of S. mansoni additionally encodes a TPC homologue. In contrast, apicomplexan parasites lack genes encoding IP3R/RyR homologues and possess only genes encoding TPC and Trp channel homologues (Toxoplasma gondii) or Trp channel homologues alone. The genomes of parasites also encode homologues of mammalian Ca2+ influx channels, including voltage-gated Ca2+ channels and plasma membrane Trp channels. The genome of S. mansoni also encodes Orai Ca2+ channel and STIM Ca2+ sensor homologues, suggesting that store-operated Ca2+ entry may occur in this parasite. Many anti-parasitic agents alter parasite Ca2+ homeostasis and some are known modulators of mammalian Ca2+ channels, suggesting that parasite Ca2+ channel homologues might be the targets of some current anti-parasitic drugs. Differences between human and parasite Ca2+ channels suggest that pathogen-specific targeting of these channels may be an attractive therapeutic prospect

Preparing for low surface brightness science with the Vera C. Rubin Observatory: characterisation of tidal features from mock images

Tidal features in the outskirts of galaxies yield unique information about their past interactions and are a key prediction of the hierarchical structure formation paradigm. The Vera C. Rubin Observatory is poised to deliver deep observations for potentially of millions of objects with visible tidal features, but the inference of galaxy interaction histories from such features is not straightforward. Utilising automated techniques and human visual classification in conjunction with realistic mock images produced using the NEWHORIZON cosmological simulation, we investigate the nature, frequency and visibility of tidal features and debris across a range of environments and stellar masses. In our simulated sample, around 80 per cent of the flux in the tidal features around Milky Way or greater mass galaxies is detected at the 10-year depth of the Legacy Survey of Space and Time (30-31 mag / sq. arcsec), falling to 60 per cent assuming a shallower final depth of 29.5 mag / sq. arcsec. The fraction of total flux found in tidal features increases towards higher masses, rising to 10 per cent for the most massive objects in our sample (M*~10^{11.5} Msun). When observed at sufficient depth, such objects frequently exhibit many distinct tidal features with complex shapes. The interpretation and characterisation of such features varies significantly with image depth and object orientation, introducing significant biases in their classification. Assuming the data reduction pipeline is properly optimised, we expect the Rubin Observatory to be capable of recovering much of the flux found in the outskirts of Milky Way mass galaxies, even at intermediate redshifts (z<0.2)

The stellar mass – physical effective radius relation for dwarf galaxies in low-density environments

ABSTRACT The scaling relation between stellar mass (M*) and physical effective radius (re) has been well studied using wide spectroscopic surveys. However, these surveys suffer from severe surface brightness incompleteness in the dwarf galaxy regime, where the relation is poorly constrained. In this study, I use a Bayesian empirical model to constrain the power-law exponent β of the M*–re relation for late-type dwarfs ($10^{7} \le$M*/M⊙$\le 10^{9}$) using a sample of 188 isolated low surface brightness (LSB) galaxies, accounting for observational incompleteness. Surprisingly, the best-fitting model (β = 0.40 ± 0.07) indicates that the relation is significantly steeper than would be expected from extrapolating canonical models into the dwarf galaxy regime. Nevertheless, the best fitting M*–re relation closely follows the distribution of known dwarf galaxies. These results indicate that extrapolated canonical models overpredict the number of large dwarf (i.e. LSB) galaxies, including ultra-diffuse galaxies (UDGs), explaining why they are overproduced by some semi-analytic models. The best-fitting model also constrains the power-law exponent of the physical size distribution of UDGs to $n\mathrm{[dex^{-1}]}\propto r_{\mathrm{ e}}^{3.54\pm 0.33}$, consistent to within 1σ of the corresponding value in cluster environments and with the theoretical scenario in which UDGs occupy the high-spin tail of the normal dwarf galaxy population.</jats:p

Observational properties of ultra-diffuse galaxies in low-density environments: field UDGs are predominantly blue and star forming

Abstract While we have learnt much about ultradiffuse galaxies (UDGs) in groups and clusters, relatively little is known about them in less dense environments. More isolated UDGs are important for our understanding of UDG formation scenarios because they form via secular mechanisms, allowing us to determine the relative importance of environmentally driven formation in groups and clusters. We have used the public Kilo-Degree Survey together with the Hyper Suprime-Cam Subaru Strategic Program to constrain the abundance and properties of UDGs in the field, targeting sources with low surface brightness (24.0 ≤ $\bar{\mu }_{\mathrm{ e},r}$ ≤ 26.5) and large apparent sizes (3.0 arcsec ≤ $\bar{r}_{\mathrm{ e},r}$ ≤ 8.0 arcsec). Accounting for several sources of interlopers in our selection based on canonical scaling relations, and using an empirical UDG model based on measurements from the literature, we show that a scenario in which cluster-like red-sequence UDGs occupy a significant number of field galaxies is unlikely, with most field UDGs being significantly bluer and showing signs of localized star formation. An immediate conclusion is that UDGs are much more efficiently quenched in high-density environments. We estimate an upper limit on the total field abundance of UDGs of 8 ± 3 × 10−3 cMpc−3 within our selection range. We also compare the total field abundance of UDGs to a measurement of the abundance of H i-rich UDGs from the literature, suggesting that they occupy at least one-fifth of the overall UDG population. The mass formation efficiency of UDGs implied by this upper limit is similar to what is measured in groups and clusters.</jats:p