Effect of radial base-plate welds on ULCF capacity of unanchored tank connections

The base of large steel liquid storage tanks can uplift during severe earthquakes, causing large inelastic rotations at the connection between the tank shell and tank base. While recent experimental studies indicate significantly higher connection rotation capacity than what is specified in the current Eurocode standard (set at 0.2 rad), additional radial base-plate welds (present in some tank connection details due to fabrication methods) have never been considered in tests. This study experimentally investigates the effects of these radial base-plate welds on the fatigue capacity of tank shell-to-base connections during uplift. Twelve tank shell-to-base connection specimens taken from existing tanks throughout Switzerland are tested at rotation ranges greater than the current Eurocode limit (eight specimens with radial welds and four specimens without radial welds). Testing indicates that tank base-plate sections containing radial welds govern the shell-to-base rotation capacity during uplift. The rotation capacity of connections containing radial welds was nearly 30% lower (on average) than equivalent connections without radial welds. This reduced capacity is directly related to the reduced base-plate ductility created by the radial weld heat affected zone. All connection capacities were far greater than the current Eurocode limit. (C) 2014 Elsevier Ltd. All rights reserved

Finite Element Analysis for Fatigue Damage Reduction in Metallic Riveted Bridges Using Pre-Stressed CFRP Plates

Many old riveted steel bridges remain operational and require retrofit to accommodate ever increasing demands. Complicating retrofit efforts, riveted steel bridges are often considered historical structures where structural modifications that affect the original construction are to be avoided. The presence of rivets along with preservation requirements often prevent the use of traditional retrofit methods, such as bonding of fiber reinforced composites, or the addition of supplementary steel elements. In this paper, an un-bonded post-tensioning retrofit method is numerically investigated using an existing railway riveted bridge geometry in Switzerland. The finite element (FE) model consists of a global dynamic model for the whole bridge and a more refined sub-model for a riveted joint. The FE model results include dynamic effects from axle loads and are compared with field measurements. Pre-stressed un-bonded carbon fiber reinforced plastic (CFRP) plates will be considered for the strengthening elements. Fatigue critical regions of the bridge are identified, and the effects of the un-bonded post-tensioning method with different prestress levels on fatigue susceptibility are explored. With an applied 40% CFRP pre-stress, fatigue damage reductions of more than 87% and 85% are achieved at the longitudinal-to-cross beam connections and cross-beam bottom flanges respectively

Self-renewing resident arterial macrophages arise from embryonic CX3CR1+ precursors and circulating monocytes immediately after birth

Resident macrophages densely populate the normal arterial wall, yet their origins and the mechanisms that sustain them are poorly understood. Here we use gene-expression profiling to show that arterial macrophages constitute a distinct population among macrophages. Using multiple fate-mapping approaches, we show that arterial macrophages arise embryonically from CX3CR1+ precursors and postnatally from bone marrow–derived monocytes that colonize the tissue immediately after birth. In adulthood, proliferation (rather than monocyte recruitment) sustains arterial macrophages in the steady state and after severe depletion following sepsis. After infection, arterial macrophages return rapidly to functional homeostasis. Finally, survival of resident arterial macrophages depends on a CX3CR1-CX3CL1 axis within the vascular niche

26th Annual Computational Neuroscience Meeting (CNS*2017): Part 3 - Meeting Abstracts - Antwerp, Belgium. 15–20 July 2017

This work was produced as part of the activities of FAPESP Research,\ud Disseminations and Innovation Center for Neuromathematics (grant\ud 2013/07699-0, S. Paulo Research Foundation). NLK is supported by a\ud FAPESP postdoctoral fellowship (grant 2016/03855-5). ACR is partially\ud supported by a CNPq fellowship (grant 306251/2014-0)

Retrospective evaluation of whole exome and genome mutation calls in 746 cancer samples

Funder: NCI U24CA211006Abstract: The Cancer Genome Atlas (TCGA) and International Cancer Genome Consortium (ICGC) curated consensus somatic mutation calls using whole exome sequencing (WES) and whole genome sequencing (WGS), respectively. Here, as part of the ICGC/TCGA Pan-Cancer Analysis of Whole Genomes (PCAWG) Consortium, which aggregated whole genome sequencing data from 2,658 cancers across 38 tumour types, we compare WES and WGS side-by-side from 746 TCGA samples, finding that ~80% of mutations overlap in covered exonic regions. We estimate that low variant allele fraction (VAF < 15%) and clonal heterogeneity contribute up to 68% of private WGS mutations and 71% of private WES mutations. We observe that ~30% of private WGS mutations trace to mutations identified by a single variant caller in WES consensus efforts. WGS captures both ~50% more variation in exonic regions and un-observed mutations in loci with variable GC-content. Together, our analysis highlights technological divergences between two reproducible somatic variant detection efforts

Ultra Low-Cycle Fatigue Behavior Comparison between Additively Manufactured and Rolled 17-4 PH (AISI 630) Stainless Steels

This study investigates the mechanical behavior of additively manufactured (AM) 17-4 PH (AISI 630) stainless steels and compares their behavior to traditionally produced wrought counterparts. The goal of this study is to understand the key parameters influencing AM 17-4 PH steel fatigue life under ULCF conditions and to develop simple predictive models for fatigue-life estimation in AM 17-4 steel components. In this study, both AM and traditionally produced (wrought) material samples are fatigue tested under fully reversed (R = −1) strain controlled (2–4% strain) loading and characterized using micro-hardness, x-ray diffraction, and fractography methods. Results indicate decreased fatigue life for AM specimens as compared to wrought 17-4 PH specimens due to fabrication porosity and un-melted particle defect regions which provide a mechanism for internal fracture initiation. Heat treatment processes performed in this work, to both the AM and wrought specimens, had no observable effect on ULCF behavior. Result comparisons with an existing fatigue prediction model (the Coffin–Manson universal slopes equation) demonstrated consistent over-prediction of fatigue life at applied strain amplitudes greater than 3%, likely due to inherent AM fabrication defects. An alternative empirical ULCF capacity equation is proposed herein to aid future fatigue estimations in AM 17-4 PH stainless steel components

Analytical and Experimental Investigation into Pre-Stressed Carbon Fiber Reinforced Polymer (CFRP) Fatigue Retrofits for Steel Waterway Lock-Gate Structures

Lock gates are an important part of the transportation infrastructure within the United States (US). Unfortunately, many existing lock gates have reached or exceeded their initial design lives and require frequent repairs to remain in service. Unscheduled repairs often increase as gates age, having a local economic impact on freight transport, which can create economic ripples throughout the nation. Metal fatigue is a key cause of unscheduled service interruptions, degrading lock gate components over time. Additionally, because lock gates are submerged during operation, crack detection prior to component failure can be difficult, and repair costs can be high. This paper presents an analytical and experimental investigation into fatigue damage within common lock gate geometries, as well as fatigue mitigation strategies with a focus on extending gate service lives. Detailed finite element analyses are combined with fatigue and fracture mechanics theories to predict critical fatigue regions within common gate details and develop retrofit strategies for mitigating fatigue cracking. Full-scale experimental fatigue testing of a critical lock gate component is conducted to provide a baseline for the evaluation of retrofit strategies. Retrofit strategies and issues in using carbon fiber reinforced polymer (CFRP) plates having optimized pre-stress levels are discussed