The importance of microbiota and terrestrial inflows in controlling seston C:N:P:O:Si:Ca:Mn:Mg:Fe:K:Na:Cl:S:Cu:Zn stoichiometry of a deep coastal fjord

Comprehensive fjord-systems represent major extensions of the coastline and are therefore important transfer zones of materials from land to ocean. Despite increased terrestrial inflows to fjords due to climate changes, we know little about the effects on the ecosystem, especially biogeochemical cycling. We present novel data on spatiotemporal variations of seston multielement stoichiometry in the Sognefjord, the second longest (204 km) and deepest (1308 m) fjord in the world, relative to environmental conditions and microbiota. Concentration of major elements was highest in the upper brackish layer whereas trace metals and minor elements were highest close to the bottom. Seasonally varying microbiota was an important part of the seston in surface waters. None of the seston C:N:P (molar) annual means at specific depths corresponded to the Redfield ratio (106:16:1). At 5 m, annual means of N/P and C/N were 8.4 and 6.5, respectively, while at depth (50–1220 m) N/P were on scale 3 times higher (21–31) and C/N 3 times lower (1.6–2.6), suggesting alternative N-sequestration mechanisms. Overall, correlations between C-Ca and C-S indicate a strong influence from calcite (CaCO3) and organosulfur producing microorganisms, while correlations between particulate Si and Mg–K–Ca–O at depth are consistent with clay and sinking diatom frustules. Mn concentrations increased strongly towards the bottom, likely from resuspension of MnO2 rich sediments and clay particles. Based on seston concentrations, we arrived at the following stoichiometric relationship: C55N16P1Si3.6Ca3.4O16Fe0.74Mn0.51Zn0.33S0.21Cu0.08Cl1.7Na0.68Mg0.71K0.37, although rarely measured, such information is a prerequisite for evaluating environmental impact on coastal ecosystems, biogeochemical cycling, pollution risk analysis and monitoring guidelines.publishedVersio

Comparing EPA production and fatty acid profiles of three Phaeodactylum tricornutum strains under western Norwegian climate conditions

Microalgae could provide a sustainable alternative to fish oil as a source for the omega-3 polyunsaturated fatty acids eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). However, growing microalgae on a large-scale is still more cost-intensive than fish oil production, and outdoor productivities vary greatly with reactor type, geographic location, climate conditions and microalgae species or even strains. The diatom Phaeodactylum tricornutum has been intensively investigated for its potential in large-scale production, due to its robustness and comparatively high growth rates and EPA content. Yet, most research have been performed in southern countries and with a single commercial P. tricornutum strain, while information about productivities at higher latitudes and of local strains is scarce. We examined the potential of the climate conditions in Bergen, western Norway for outdoor cultivation of P. tricornutum in flat panel photobioreactors and cultivated three different strains simultaneously, one commercial strain from Spain (Fito) and two local isolates (M28 and B58), to assess and compare their biomass and EPA productivities, and fatty acid (FA) profiles. The three strains possessed similar biomass productivities (average volumetric productivities of 0.20, 0.18, and 0.21 g L− 1 d− 1), that were lower compared to productivities reported from southern latitudes. However, EPA productivities differed between the strains (average volumetric productivities of 9.8, 5.7 and 6.9 mg L− 1 d− 1), due to differing EPA contents (average of 4.4, 3.2 and 3.1% of dry weight), and were comparable to results from Italy. The EPA content of strain Fito of 4.4% is higher than earlier reported for P. tricornutum (2.6–3.1%) and was only apparent under outdoor conditions. A principal component analysis (PCA) of the relative FA composition revealed strain-specific profiles. However, including data from laboratory experiments, revealed more significant differences between outdoor and laboratory-grown cultures than between the strains, and higher EPA contents in outdoor grown cultures.publishedVersio

Marine Benthic Diatoms Contain Compounds Able to Induce Leukemia Cell Death and Modulate Blood Platelet Activity

In spite of the high abundance and species diversity of diatoms, only a few bioactive compounds from them have been described. The present study reveals a high number of mammalian cell death inducing substances in biofilm-associated diatoms sampled from the intertidal zone. Extracts from the genera Melosira, Amphora, Phaeodactylum and Nitzschia were all found to induce leukemia cell death, with either classical apoptotic or autophagic features. Several extracts also contained inhibitors of thrombin-induced blood platelet activation. Some of this activity was caused by a high content of adenosine in the diatoms, ranging from 0.07 to 0.31 μg/mg dry weight. However, most of the bioactivity was adenosine deaminase-resistant. An adenosine deaminase-resistant active fraction from one of the extracts was partially purified and shown to induce apoptosis with a distinct phenotype. The results show that benthic diatoms typically found in the intertidal zone may represent a richer source of interesting bioactive compounds than hitherto recognized

Bioprospecting North Atlantic microalgae with fast growth and high polyunsaturated fatty acid (PUFA) content for microalgae-based technologies

Microalgae are considered to be an important and sustainable alternative to fish oil as a source for the polyunsaturated fatty acids (PUFAs) eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). Due to their health benefits, there is an increasing interest in the commercial application of these fatty acids (FA) to health and dietary products, and to aquaculture feeds. However, FA from microalgae are still expensive to produce compared to fish or plant oils. With only a few microalgal strains being cultivated on a large scale for commercial PUFA production, prospecting for new, robust and fast-growing strains with increased PUFA content is essential in order to reduce production costs. Microalgae from northern high latitudes, exposed to cold temperatures, may be especially promising candidates as previous studies have shown increasing unsaturation of FA in response to decreasing growth temperatures in different microalgae, most likely to maintain membrane fluidity and function. We have designed a screening pipeline, targeting a focused search and selection for marine microalgal strains from extreme North Atlantic locations with high robustness and biomass production, and increased levels of EPA and DHA. The pipeline includes a rational sampling plan, isolation and cultivation of clonal strains, followed by a batch growth experiment designed to obtain information on robustness, growth characteristics, and the FA content of selected isolates during both nutrient replete exponential cultivation and nutrient limited stationary cultivation. A number of clonal cultures (N = 149) have been established, and twenty of these strains have been screened for growth and FA content and composition. Among those strains, three showed growth rates ≥ 0.7 d− 1 at temperatures of 15 °C or below, and high amounts of EPA (> 3% DW), suggesting their potential as candidates for large scale production

Specific metabolites in a Phaeodactylum tricornutum strain Isolated from Western Norwegian fjord water

We have searched for special characteristics in growth, protein expression, fatty acids and volatile organic compounds (VOCs) in a local Phaeodactylum tricornutum Bohlin strain (Bergen Marine Biobank), by comparing it with a common accession strain (CCAP). Differences in growth and expressed proteins were detected between the BMB strain and the CCAP strain, and the BMB strain reached the highest cell densities under the given growth conditions. Fatty acid (FA) analyses showed highest relative eicosapentaenoic acid (EPA) levels in the exponential phase (25.73% and 28.31%), and highest levels of palmitoleic acid (16:1 n-7) in the stationary phase (46.36% and 43.66%) in the BMB and CCAP strain, respectively. The most striking finding of the VOCs analyses was the relatively high levels of ectocarpene, 6-((1E)-butenyl)-1,4-cycloheptadiene, hormosirene, and desmarestene and structurally related compounds, which were exclusively detected in the BMB strain. Many of the VOCs detected in the CCAP and, in particular, in the BMB strain have been reported as antimicrobial agents. We suggest that the array of pheromones and antimicrobial substances could be part of an allelopathic strategy of the BMB strain, dominated by oval cells, thus reflecting the benthic life stage of this morphological form. These findings show the potential for bioactive metabolites in the BMB strain

The importance of microbiota and terrestrial inflows in controlling seston C:N:P:O:Si:Ca:Mn:Mg:Fe:K:Na:Cl:S:Cu:Zn stoichiometry of a deep coastal fjord

Comprehensive fjord-systems represent major extensions of the coastline and are therefore important transfer zones of materials from land to ocean. Despite increased terrestrial inflows to fjords due to climate changes, we know little about the effects on the ecosystem, especially biogeochemical cycling. We present novel data on spatiotemporal variations of seston multielement stoichiometry in the Sognefjord, the second longest (204 km) and deepest (1308 m) fjord in the world, relative to environmental conditions and microbiota. Concentration of major elements was highest in the upper brackish layer whereas trace metals and minor elements were highest close to the bottom. Seasonally varying microbiota was an important part of the seston in surface waters. None of the seston C:N:P (molar) annual means at specific depths corresponded to the Redfield ratio (106:16:1). At 5 m, annual means of N/P and C/N were 8.4 and 6.5, respectively, while at depth (50–1220 m) N/P were on scale 3 times higher (21–31) and C/N 3 times lower (1.6–2.6), suggesting alternative N-sequestration mechanisms. Overall, correlations between C-Ca and C-S indicate a strong influence from calcite (CaCO3) and organosulfur producing microorganisms, while correlations between particulate Si and Mg–K–Ca–O at depth are consistent with clay and sinking diatom frustules. Mn concentrations increased strongly towards the bottom, likely from resuspension of MnO2 rich sediments and clay particles. Based on seston concentrations, we arrived at the following stoichiometric relationship: C55N16P1Si3.6Ca3.4O16Fe0.74Mn0.51Zn0.33S0.21Cu0.08Cl1.7Na0.68Mg0.71K0.37, although rarely measured, such information is a prerequisite for evaluating environmental impact on coastal ecosystems, biogeochemical cycling, pollution risk analysis and monitoring guidelines.publishedVersio

Comparing EPA production and fatty acid profiles of three Phaeodactylum tricornutum strains under western Norwegian climate conditions

Microalgae could provide a sustainable alternative to fish oil as a source for the omega-3 polyunsaturated fatty acids eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). However, growing microalgae on a large-scale is still more cost-intensive than fish oil production, and outdoor productivities vary greatly with reactor type, geographic location, climate conditions and microalgae species or even strains. The diatom Phaeodactylum tricornutum has been intensively investigated for its potential in large-scale production, due to its robustness and comparatively high growth rates and EPA content. Yet, most research have been performed in southern countries and with a single commercial P. tricornutum strain, while information about productivities at higher latitudes and of local strains is scarce. We examined the potential of the climate conditions in Bergen, western Norway for outdoor cultivation of P. tricornutum in flat panel photobioreactors and cultivated three different strains simultaneously, one commercial strain from Spain (Fito) and two local isolates (M28 and B58), to assess and compare their biomass and EPA productivities, and fatty acid (FA) profiles. The three strains possessed similar biomass productivities (average volumetric productivities of 0.20, 0.18, and 0.21 g L− 1 d− 1), that were lower compared to productivities reported from southern latitudes. However, EPA productivities differed between the strains (average volumetric productivities of 9.8, 5.7 and 6.9 mg L− 1 d− 1), due to differing EPA contents (average of 4.4, 3.2 and 3.1% of dry weight), and were comparable to results from Italy. The EPA content of strain Fito of 4.4% is higher than earlier reported for P. tricornutum (2.6–3.1%) and was only apparent under outdoor conditions. A principal component analysis (PCA) of the relative FA composition revealed strain-specific profiles. However, including data from laboratory experiments, revealed more significant differences between outdoor and laboratory-grown cultures than between the strains, and higher EPA contents in outdoor grown cultures