118 research outputs found
Synthesis and characterization of a pyrochlore solid solution in the Na2OâBi2O3âTiO2 system
The compositional limits of a previously reported (J. Am. Ceram. Soc., 61, 5â8. (1978)) but relatively unstudied sodiumâbismuth titanate pyrochlore solid solution are revised and their electrical properties presented. The pyrochlore solid solution we report forms via a different mechanism to that originally reported and occurs in a different location within the Na2OâBi2O3âTiO2 ternary system. In both cases, relatively large amounts of vacancies are required on the Aâsites and on the oxygen sites, similar to that reported for undoped âBi2Ti2O7â pyrochlore. In contrast to âBi2Ti2O7â, this ternary pyrochlore solid solution can be prepared and ceramics sintered using conventional solidâstate methods; however, the processing requires several challenges to be overcome to obtain dense ceramics. This cubic pyrochlore series has low electrical conductivity (and does not exhibit any evidence of oxideâion conduction) and exhibits relaxor ferroelectric behavior with a broad permittivity maximum of ~100 near room temperature. Variable temperature neutron diffraction data do not provide any conclusive evidence for a phase transition in the pyrochlore solid solution between ~4 and 873 K
Berry size variability in Vitis vinifera L.
In order to study the mechanisms of berry growth in Vitis vinifera L. we analysed cell division and pericarp enlargement of 6 genotypes showing high variability in berry size (range: 49-90 %. Both, cell number and cell volume were involved in the small-sized berries of the flb mutant producing wild-type-like berries. The differences of berry size observed in other small-sized clones were only due to cell expansion. The data suggest that the variability of berry size in V. vinifera cultivars predominantly results from modification of cell enlargement. Conversely, in wild-type-like berries, the variability of berry size could result from both, cell division and cell enlargement.
MuSCA: A multi-scale source-sink carbon allocation model to explore carbon allocation in plants. An application to static apple tree structures
Background and aims: Carbon allocation in plants is usually represented at a topological scale, specific to each model. This makes the results obtained with different models, and the impact of their scales of representation, difficult to compare. In this study, we developed a multi-scale carbon allocation model (MuSCA) that allows the use of different, user-defined, topological scales of a plant, and assessment of the impact of each spatial scale on simulated results and computation time. Methods: Model multi-scale consistency and behaviour were tested on three realistic apple tree structures. Carbon allocation was computed at five scales, spanning from the metamer (the finest scale, used as a reference) up to first-order branches, and for different values of a sap friction coefficient. Fruit dry mass increments were compared across spatial scales and with field data. Key Results: The model was able to represent effects of competition for carbon assimilates on fruit growth. Intermediate friction parameter values provided results that best fitted field data. Fruit growth simulated at the metamer scale differed of ~1 % in respect to results obtained at growth unit scale and up to 60 % in respect to first order branch and fruiting unit scales. Generally, the coarser the spatial scale the more predicted fruit growth diverged from the reference. Coherence in fruit growth across scales was also differentially impacted, depending on the tree structure considered. Decreasing the topological resolution reduced computation time by up to four orders of magnitude. Conclusions: MuSCA revealed that the topological scale has a major influence on the simulation of carbon allocation. This suggests that the scale should be a factor that is carefully evaluated when using a carbon allocation model, or when comparing results produced by different models. Finally, with MuSCA, trade-off between computation time and prediction accuracy can be evaluated by changing topological scales
Cellular localisation of VvRops and VvRabA5e, small GTPases developmentally regulated in grape berries
VvRops, in particular VvRop9, and VvRabA5e are small GTPases which are developmentally regulated in grape berries. In an attempt to help elucidate the role of these proteins during fruit development and ripening, we investigated their localisation in the fruit by immunocytofluorescence. These proteins were observed at a perinuclear location, at cell periphery and around vesicles. In particular VvRops were found to be located in the nucleus and likely on the plasma membrane. VvRop9 and VvRabA5e cDNAs were introduced separately into S. cerevisiae mutants with RHO1 and YPT31/YPT32 defective genes respectively. Neither cDNAs could complement these temperature-sensitive mutants, suggesting that the functions of the VvRop9 and VvRabA5e genes in grapevine likely differ from the functions of RHO1 and YPT31/YPT32 genes in yeast.
A Multi-Scale Model to explore Carbon Allocation in Plants
International audienceUnderstanding and simulating carbon allocation in plants is necessary to distribute carbohydrates among growing and competing organs and to predict plant growth and structure development in relation to climatic conditions. In this context several carbon allocation models have been developed but no clear consensus exists on (i) the most appropriate topological scale (organ, metamer, compartment...) to represent this process on complex plant structures, (ii) the importance of distances between organs in carbon transport, (iii) the priorities in carbon allocation among plant parts, that can depend on growth stages. Multi-scale tree graph (MTG) is a formalism allowing the representation of geometry and topology of a tree structure at different scales. In this study, several models were implemented to compute carbon allocation at user-defined spatial scales by using the MTG formalism. This allows multiple scales (e.g. metamer, growing unit, branch) to be combined during the computation of carbon allocation (e.g. allocation first within leafy shoots at metamer scale and then between growing units). The model describes carbon transport, taking into account the distances between sources and sinks, the strength of the sinks and the available carbohydrates, following the equations of the SIMWAL and QualiTree models. Simulations on simplified branching structures, represented at different scales, showed how the scales chosen to represent the system influence the results of predicted carbon allocation. This modelling approach will be first applied to apple tree to analyze the impact of the scale of representation (branch, growth unit, metamer, and inflorescence) on the predicted fruit growth variability which, in turn, will be compared with field observations. The present work is available through the OpenAlea platform and provides existing Functional Structural Plant Models with a new generic model to simulate carbon allocation in plants depending on user-defined biological hypotheses, such as the choice of the scale of representation or the effect of distance
The GCC repeat length in the 5'UTR of MRP1 gene is polymorphic: a functional characterization of its relevance for cystic fibrosis
BACKGROUND: Among the members of the ATP binding cassette transporter superfamily, MRPs share the closest homology with the CFTR protein, which is defective in CF disease. MRP1 has been proposed as a potential modifier gene and/or as novel target for pharmacotherapy of CF to explain the clinical benefits observed in some CF patients treated with the macrolide AZM. The 5'UTR of the MRP1 gene contains a GCC triplet repeat that could represent a polymorphic site and affect the activity of the promoter. METHODS: The MRP1 5' flanking region was amplified by PCR from 36 CF patients and 100 non-CF subjects and the number of GCC triplets of each allele was determined by sequence and electrophoretic analysis. We performed gene reporter studies in CF airway epithelial cells 16HBE14o-AS3, in basal conditions and in the presence of AZM. RESULTS: We found that the GCC repeat is polymorphic, ranging from 7 to 14 triplets either in CF or in non-CF subjects. Our data are preliminary and have to be confirmed on a larger population of CF subjects. The transcriptional activity of the proximal MRP1 5' regulatory region revealed no statistically significant correlations between the number of repeats and treatment with AZM. CONCLUSION: We identified a novel polymorphism in the 5'UTR of MRP1 gene that provides multiple alleles in a gene relevant for multidrug resistance as well as for CF, determining that this region is transcriptionally active and that this activity does not appear to be influenced by AZM treatment
Use of the time constant related parameter fmax to calculate the activation energy of bulk conduction in ferroelectrics
The activation energy associated with bulk electrical conduction in functional materials is an important quantity which is often determined by impedance spectroscopy using an Arrhenius-type equation. This is achieved by linear fitting of bulk conductivity obtained from complex (Z*) impedance plots versus T-1 which gives an activation energy Ea(Ï) or by linear fitting of the characteristic frequency fmax obtained from the large Debye peak in Mââ-logf spectroscopic plots against T-1 which gives an activation energy Ea(fmax). We report an analysis of Ea(Ï) and Ea(fmax) values for some typical non-ferroelectric and ferroelectric materials and employ numerical simulations to investigate combinations of different conductivity-temperature and permittivity-temperature profiles on the logfmax â T-1 relationship and Ea(fmax). Results show the logfmax â T-1 relationship and Ea(fmax) are strongly dependent on the permittivity-temperature profile and the temperature range measured relative to Tm (temperature of the permittivity maximum). Ferroelectric materials with a sharp permittivity peak can result in non-linear logfmax â T-1 plots in the vicinity of Tm. In cases where data are obtained either well above or below Tm, linear logfmax â T-1 plots can be obtained but overestimate or underestimate the activation energy for conduction, respectively. It is therefore not recommended to use Ea(fmax) to obtain the activation energy for bulk conduction in ferroelectric materials, instead Ea(Ï) should be used
Exploring carbon allocation with a multi-scale model: the case of apple
UMR AGAP - Ă©quipe AFEF - Architecture et fonctionnement des espĂšces fruitiĂšresUnderstanding the allocation of carbohydrates among organs is necessary to predict plant growth in relation to climatic conditions and agronomic practices. Despite the large number of studies on the subject of carbon allocation, no clear consensus exists on (i) the most appropriate topological scale (organ, metamer, compartment...) to represent this process on complex plant structures, and (ii) the importance of distances between organs in carbon transport. In this study, we implemented a generic source-sink based carbon allocation model, following the equation of the SIMWAL model, that takes into account the distances between sources and sinks, the sink strength and the availability of carbohydrates from photosynthesis. Our model makes use of multi-scale tree graph (MTG) to represent geometry and topology of a tree structure at different scales. Starting from the description of a plant at a given scale (e.g. metamer and growing unit scales), we defined additional grouping criteria (fruiting branches and main axis) that were used to represent the plant structure, and the process of carbon allocation at different spatial resolutions. Generic functions to determine the biomass and carbon demand of the individual organs described in an MTG were implemented and calibrated for apple trees (Fuji variety) by means of age and organ type dependent allometric equations and maximum potential Relative Growth Rate curves (RGR) obtained in a field experiment. Photosynthesis for individual leaves of the input MTG was estimated by means of a radiative model (RATP). The model was then applied to architectural mock-ups in the MTG format produced by the MappleT model, representing trees with high and low fruit loads. Simulations on simplified plant structures qualitatively showed the influence of the scale of representation and of the distance parameter on the predicted carbon allocation. In order to test assumptions regarding the effect of distance, the source-sink behavior and the suitability of the alternative scales of representation for predicting carbon allocation, the variability and spatial distribution of the simulated RGR were compared to field observations. Finally, a benchmarking was performed to compare the computational efficiency of the model when running at different scales. The presented multiscale model provides a framework to re-interpret the plant topology in order to test the influence of some assumptions at the basis of the carbon allocation process, such as branch autonomy or the effect of distance. It is also a mean to investigate the trade-offs between the detail at which a plant is described, and the accuracy and computational efficiency in predicting carbon allocation. The present work was developed on the OpenAlea platform, and will provide existing Functional Structural Plant Models with a new generic model to simulate carbon allocation in plants
Early bronchopulmonary involvement in Crohn disease: a case report
BACKGROUND: Bronchopulmonary manifestations of Crohn disease have been rarely described in children, including both subclinical pulmonary involvement and severe lung disease. CASE PRESENTATION: A 6.5-year-old girl is described with early recurrent bronchopulmonary symptoms both at presentation and in the quiescent phase of Crohn disease. Pulmonary function tests (lung volumes and flows, bronchial reactivity and carbon monoxide diffusing capacity) were normal. Bronchoalveolar cytology showed increased (30%) lymphocyte counts and bronchial biopsy revealed thickening of basal membrane and active chronic inflammation. CONCLUSIONS: Clinical and histological findings in our young patient suggest involvement of both distal and central airways in an early phase of lung disease. The pathogenesis of Crohn disease-associated lung disorders is discussed with reference to the available literature. A low threshold for pulmonary evaluation seems to be advisable in all children with CD
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