Comprehensive metabolite profiling of Plantaginis Semen using ultra high performance liquid chromatography with electrospray ionization quadrupole time-of-flight tandem mass spectrometry coupled with elevated energy technique

Plantaginis Semen is commonly used in traditional medicine to treat edema, hypertension, and diabetes. The commercially available Plantaginis Semen in China mainly comes from three species. To clarify the chemical composition and distinct different species of Plantaginis Semen, we established a metabolite profiling method based on ultra high performance liquid chromatography with electrospray ionization quadrupole time-of-flight tandem mass spectrometry coupled with elevated energy technique. A total of 108 compounds, including phenylethanoid glycosides, flavonoids, guanidine derivatives, terpenoids, organic acids, and fatty acids, were identified from Plantago asiatica L., P. depressa Willd., and P. major L. Results showed significant differences in chemical components among the three species, particularly flavonoids. This study is the first to provide a comprehensive chemical profile of Plantaginis Semen, which could be involved into the quality control, medication guide, and developing new drug of Plantago seeds

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Not AvailableIn molecular biology, we most often handle with bacmid when working with baculovirus expression system. The cloning involves a two-step process and the recombinant bacmids are screened by bluewhite lac operon system, white colonies are thought to be positive for transposition. But in our study with PCR based approach, we found that not all the white colonies are recombinants. Hence, it is necessary to be cautious while screening for recombinant bacmids and may be it is good too screen by two-way approach, by blue-white lac operon system followed by PCR approach using one bacmid specific and one gene specific primers.Not Availabl

Ortho-Metallated triphenylphosphine chalcogenide complexes of platinum and palladium: synthesis and catalytic activity

Treatment of [PtI2(COD)] (COD = 1,5-cyclooctadiene) with 2-LiC6H4P(S)Ph2 gives the complex cis-[Pt{κ2-2-C6H4P(S)Ph2} 2] (1) containing a pair of ortho-metallated triphenylphosphine sulfide rings. The selenium counterpart, [Pt{κ2-2-C 6H4P(Se)Ph2}2] (2), which exists as cis- and trans-isomers in solution, and the palladium analogues, cis-[Pd{κ2-2-C6H4P(X)Ph2} 2] [X = S (3), Se (4)], are obtained by transmetallation of [MCl 2(COD)] with the organotin reagent 2-Me3SnC 6H4P(X)Ph2 in a 1:2 mol ratio. The reaction of [PdCl2(COD)] with 2-Me3SnC6H 4P(X)Ph2 in a 1:1 mol ratio, and the reaction of 3 with palladium(ii) acetate, give dinuclear, anion-bridged complexes [Pd 2(μ-Cl)2{κ2-2-C6H 4P(X)Ph2}2] [X = S (5), Se (6)] and [Pd 2(μ-OAc)2{κ2-2-C6H 4P(S)Ph2}2] (8), respectively. Complexes 5 and 8 could not be made directly from triphenylphosphine sulfide by standard ortho-palladation procedures. The bridging framework in complexes 5 and 6 is cleaved by tertiary phosphines to give mononuclear derivatives [PdCl{κ2-2-C6H4P(X)Ph2} (PR3)] [X = S, R = Ph (11); X = Se, R = Ph (13); X = Se, R = 4-tolyl (15)]. The selenium-containing compounds 13 and 15 decompose slowly in solution giving dinuclear complexes [PdCl(μ2-Se-κ2-P,Se-2- SeC6H4PPh2)PdCl(μ-2-C6H 4PPh2)(PR3)] [R = Ph (14), 4-tolyl (16)]. The structure of complex 16 establishes that the bridging 2-C6H 4PPh2 group is generated by reduction of the phosphine selenide unit, not by ortho-metallation of the coordinated triphenylphosphine. The chloro-bridges of 5 and 6 are also cleaved by acetylacetonate (acac) and deprotonated Schiff bases forming mononuclear species [Pd{κ2-2- C6H4P(X)Ph2}L2] [L2 = acac, X = S (9), Se (10); L2 = 2-OC6H4CHNC 6H4-4-R, X = S, R = OMe (17), NO2 (18); X = Se, R = OMe (19), NO2 (20)]

ortho -Metallated triphenylphosphine chalcogenide complexes of platinum and palladium: synthesis and catalytic activity

Treatment of [PtI2(COD)] (COD = 1,5-cyclooctadiene) with 2-LiC6H4P(S)Ph2 gives the complex cis-[Pt{κ2-2-C6H4P(S)Ph2} 2] (1) containing a pair of ortho-metallated triphenylphosphine sulfide rings. The selenium counterpart, [Pt{κ2-2-C 6H4P(Se)Ph2}2] (2)