Design of Wireless Sensor Nodes for Structural Health Monitoring applications

Enabling low-cost distributed monitoring, wireless sensor networks represents an interesting solution for the implementation of structural health monitoring systems. This work deals with the design of wireless sensor networks for health monitoring of civil structures, specifically focusing on node design in relation to the requirements of different structural monitoring application classes. Design problems are analysed with specific reference to a large-scale experimental setup (the long-term structural monitoring of the Basilica S. Maria di Collemaggio, L’Aquila, Italy). Main limitations emerged are highlighted, and adopted solution strategies are outlined, both in the case of commercial sensing platform and of full custom solutions

Competing symmetries and broken bonds in superconducting vortex-antivortex molecular crystals

Hall probe microscopy has been used to image vortex-antivortex molecules induced in superconducting Pb films by the stray fields from square arrays of magnetic dots. We have directly observed spontaneous vortex-antivortex pairs and studied how they interact with added free (anti)fluxons in an applied magnetic field. We observe a variety of phenomena arising from competing symmetries which either drive added antivortices to join antivortex shells around dots or stabilize the translationally symmetric antivortex lattice between the dots. Added vortices annihilate antivortex shells, leading first to a stable “nulling state” with no free fluxons and then, at high densities, to vortex shells around the dots stabilized by the asymmetric antipinning potential. Our experimental findings are in good agreement with Ginzburg-Landau calculations

Erratum to: 23 November 1980 Irpinia–Basilicata earthquake (Southern Italy): towards a full knowledge of the seismic effects

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An integrated vibration-image procedure for damage identification in steel trusses

The paper deals with structural damage identification in steel trusses. Classical procedure based on dynamic measurements able to detect flexibility changes are complemented with data predicting cracks and their evaluating by image processing. The procedure proposes first a damage index, the Stiffness Reduction Factor (SRF), evaluated on the basis of the error between the predictive truss model and the experimental modal model. Then, a nonlinear FEM model is used to determine fatigue cracks in the truss nodes which are compared with the observed ones determined by image processing. A real case study, the Quisi bridge located in Spain, is used to show the potentiality of the procedure

Neuronal and psychological underpinnings of pathological gambling

Like in the case of drugs, gambling hijacks reward circuits in a brain which is not prepared to receive such intense stimulation. Dopamine is normally released in response to reward and uncertainty in order to allow animals to stay alive in their environment – where rewards are relatively unpredictable. In this case, behavior is regulated by environmental feedbacks, leading animals to persevere or to give up. In contrast, drugs provide a direct, intense pharmacological stimulation of the dopamine system that operates independently of environmental feedbacks, and hence causes “motivational runaways”. With respect to gambling, the confined environment experienced by gamblers favors the emergence of excitatory conditioned cues, so that positive feedbacks take over negative feedbacks. Although drugs and gambling may act differently, their abnormal activation of reward circuitry generates an underestimation of negative consequences and promotes the development of addictive/compulsive behavior. In Parkinson’s and Huntington’s disease, dopamine-related therapies may disrupt these feedbacks on dopamine signalling, potentially leading to various addictions, including pathological gambling. The goal of this Research Topic is to further our understanding of the neurobiological mechanisms underlying the development of pathological gambling. This eBook contains a cross-disciplinary collection of research and review articles, ranging in scope from animal behavioral models to human imaging studies

A fragment-based virtual screening approach to identify e-cadherin lingands

Cadherins are calcium-dependent cell-cell adhesion proteins which are overexpressed in several solid tumors [1]. They contain an extracellular region consisting of five immunoglobulin-like domains that extend from the cell surface. Recent crystal structures have shown that classical cadherins dimerize through a \u2018strand-swap\u2019 trans-adhesive interface involving the N-terminal EC1 domains of two cadherins on adjacent cells [2, 3]. Despite a growing interest in the field, the rational design of small ligands targeting cadherins is still in a very early stage. Recently, our group set up a docking protocol (Glide v 5.7) to rationally design peptidomimetic ligands mimicking the N- and E-cadherin adhesive homodimer interface. Accordingly, the first mimics based on the tetrapeptide sequence Asp1-Trp2-Val3-Ile4 (DWVI) of the N-terminal adhesion arm were achieved and proved to inhibit the adhesion of epithelial ovarian cancer cells with millimolar potency [4]. Herein, a fragment-based virtual screening approach was applied to identify novel chemical entries targeting the DWVI binding site. Commercially available Maybridge and Life chemicals collections were used. The most promising fragments identified by the docking calculations were purchased and their binding to E-cadherin was evaluated by means of STD (Saturation Transfer Difference) NMR experiments. Acknowledgements: We thank MIUR (PRIN 2015 project 20157WW5EH) for financial support. ____ [1] G. Berx, F. van Roy, Cold Spring Harbor Perspectives in Biology 2009, 1, a003129. [2] D. Leckband, S. Sivasankar, Curr. Opin. Cell Biol. 2012, 24, 620-627. [3] J. Vendome, K. Felsovalyi, H. Song, Z. Yang, X. Jin, J. Brasch, O. J. Harrison, G. Ahlsen, F. Bahna, A. Kaczynska, P. S. Katsamba, D. Edmond, W. L. Hubbell, L. Shapiro, B. Honig, PNAS 2014, 111, E4175-E4184. [4] F. Doro, C. Colombo, C. Alberti, D. Arosio, L. Belvisi, C. Casagrande, R. Fanelli, L. Manzoni, E. Parisini, U. Piarulli, E. Luison, M. Figini, A. Tomassetti, M. Civera, Org. Biomol. Chem. 2015, 13, 2570-2573

NMR interaction studies of Neu5Ac-α-(2,6)-Gal-β-(1-4)-GlcNAc with influenza-virus hemagglutinin expressed in transfected human cells

The emergence of escape-mutants of influenza hemagglutinin (HA) following vaccination compels the yearly re-formulation of flu vaccines. Since binding the sialic acid receptor remains in all cases essential for infection, small-molecule inhibitors of HA binding to sialic acid could be interesting therapeutic complements or alternatives to immuno-prophylaxis in the control of flu epidemics. In this work, we made use of NMR spectroscopy to study the interaction between a derivative of sialic acid (the Neu5Ac-\u3b1-(2,6)-Gal-\u3b2-(1-4)-GlcNAc trisaccharide) and HAs (H1 and H5) from human and avian strains of influenza virus, directly expressed on the surface of stable transfected 293 T human cells. The HAs were shown to retain their native trimeric conformation and binding properties. Exploiting the magnetization transfer between the proteins and the ligand, we obtained evidence of the binding event and mapped the (non-identical) sugar epitopes recognized by the two HA species. The rapid and reliable method for screening sialic acid-related HA ligands we have developed could yield useful information for an efficient drug design

Acceleration of hemiacetal cleavage through hydrogen bonding : a new synthetic catalyst with balanced conformational flexibility and preorganization

Hemiacetal cleavage catalyst 1 was designed, synthesized, and shown to be effective in promoting glycolaldehyde dimer dissociation and tetramethylglucose mutarotation

A method for characterizing the stability of light sources

We describe a method for measuring small fluctuations in the intensity of a laser source with a resolution of 10⁻⁴. The current signal generated by a PIN diode is passed to a front-end electronics that discriminates the AC from the DC components, which are physically separated and propagated along circuit paths with different gains. The gain long the AC signal path is set one order of magnitude larger than that along the DC signal path in such a way to optimize the measurement dynamic range. We then derive the relative fluctuation signal by normalizing the input-referred AC signal component to its input-referred DC counterpart. In this way the fluctuation of the optical signal waveform relative to the mean power of the laser is obtained. A "Noise-Scattering-Pattern method" and a "Signal-Power-Spectrum method" are then used to analyze the intensity fluctuations from three different solid-state lasers. This is a powerful tool for the characterization of the intensity stability of lasers. Applications are discussed

Insulin-stimulated phosphorylation of endothelial nitric oxide synthase at serine-615 contributes to nitric oxide synthesis

Insulin stimulates endothelial NO (nitric oxide) synthesis via PKB (protein kinase B)/Akt-mediated phosphorylation and activation of eNOS (endothelial NO synthase) at Ser-1177. In previous studies, we have demonstrated that stimulation of eNOS phosphorylation at Ser-1177 may be required, yet is not sufficient for insulin-stimulated NO synthesis. We therefore investigated the role of phosphorylation of eNOS at alternative sites to Ser-1177 as candidate parallel mechanisms contributing to insulin-stimulated NO synthesis. Stimulation of human aortic endothelial cells with insulin rapidly stimulated phosphorylation of both Ser-615 and Ser-1177 on eNOS, whereas phosphorylation of Ser-114, Thr-495 and Ser-633 was unaffected. Insulin-stimulated Ser-615 phosphorylation was abrogated by incubation with the PI3K (phosphoinositide 3-kinase) inhibitor wortmannin, infection with adenoviruses expressing a dominant-negative mutant PKB/Akt or pre-incubation with TNFα (tumour necrosis factor α), but was unaffected by high culture glucose concentrations. Mutation of Ser-615 to alanine reduced insulin-stimulated NO synthesis, whereas mutation of Ser-615 to aspartic acid increased NO production by NOS in which Ser-1177 had been mutated to an aspartic acid residue. We propose that the rapid PKB-mediated stimulation of phosphorylation of Ser-615 contributes to insulin-stimulated NO synthesis