159 research outputs found
Understanding the reproductive behavior and population condition of the sandbar shark (Carcharhinus plumbeus) in the western North Atlantic: A molecular approach to conservation and management
The sandbar shark, Carcharhinus plumbeus, has a discontinuous cosmopolitan distribution and is exploited throughout much of its range. In the western North Atlantic, it constitutes the majority of the directed commercial fishery. The stock has declined greatly since the fisheries\u27 inception and has not shown signs of recovery despite the implementation of management practices. Like many highly vagile marine species, it is difficult to obtain information about the sandbar shark through direct observation. Therefore, the goal of this dissertation is to use a molecular approach to examine aspects of behavior and reproduction, providing information useful in conservation and management. to this end, I examine the prevalence of genetic polyandry in the western North Atlantic and estimate effective population size and effective number of breeders for the Delaware Bay and Eastern Shore of Virginia nursery grounds. In addition, I look at patterns of philopatry and reproductive periodicity, while on a worldwide scale, assessing both historical and contemporary gene flow. Paternity analysis using microsatellite markers reveals that most females are mate with multiple males during one reproductive period. Despite the high prevalence of genetic polyandry, no direct benefits are detected. The data, however, suggest that males benefit by excluding other males from mating, intimating strong intrasexual competition. The effective number of breeders per nursery ground, estimated using the linkage disequilibrium method, is fairly consistent across years. Comparisons with census size estimates made for Delaware Bay reveal that the two measurements are tightly coupled. The ratio of effective size to census size is 0.45 or higher. This suggests that monitoring of effective population size may be a useful methodology for tracking abundance, and that exploitation may have a direct negative impact on the level of genetic variance. The results suggest that females may stray between nursery grounds found in Delaware Bay, the Eastern Shore lagoons and Chesapeake Bay, as phi st values are nonsignificant and kin groups are detected between as well as within samples. However, true kin groups can not be distinguished from erroneous kin groups because sample size is too small and the loci employed do not have enough power. Even so, the results suggest that female reproductive periodicity in this species needs to be reevaluated. Different patterns of historical dispersal and contemporary gene flow are observed when markers with different modes of inheritance are used to evaluate historical phylogeography. The results suggest that, although females show regional phylopatry, pulses of female dispersal during the Pleistocene may have created the species\u27 current distribution. This dynamic may have been mediated by the changing distribution of nursery habitat caused by the rise and fall of sea level associated with climate change rather than by fluctuating temperature. This idea is supported by the results, which suggest that male mediated gene flow persists long after female gene flow has stopped
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These arenβt the loci youβre looking for: Principles of effective SNP filtering for molecular ecologists
Sequencing reducedβrepresentation libraries of restriction siteβassociated DNA (RADseq) to identify single nucleotide polymorphisms (SNPs) is quickly becoming a standard methodology for molecular ecologists. Because of the scale of RADseq data sets, putative loci cannot be assessed individually, making the process of filtering noise and correctly identifying biologically meaningful signal more difficult. Artefacts introduced during library preparation and/or bioinformatic processing of SNP data can create patterns that are incorrectly interpreted as indicative of population structure or natural selection. Therefore, it is crucial to carefully consider types of errors that may be introduced during laboratory work and data processing, and how to minimize, detect and remove these errors. Here, we discuss issues inherent to RADseq methodologies that can result in artefacts during library preparation and locus reconstruction resulting in erroneous SNP calls and, ultimately, genotyping error. Further, we describe steps that can be implemented to create a rigorously filtered data set consisting of markers accurately representing independent loci and compare the effect of different combinations of filters on four RAD data sets. At last, we stress the importance of publishing raw sequence data along with final filtered data sets in addition to detailed documentation of filtering steps and quality control measures
Kin-Aggregations Explain Chaotic Genetic Patchiness, a Commonly Observed Genetic Pattern, in a Marine Fish
The phenomenon of chaotic genetic patchiness is a pattern commonly seen in marine organisms, particularly those with demersal adults and pelagic larvae. This pattern is usually associated with sweepstakes recruitment and variable reproductive success. Here we investigate the biological underpinnings of this pattern in a species of marine goby Coryphopterus personatus. We find that populations of this species show tell-tale signs of chaotic genetic patchiness including: small, but significant, differences in genetic structure over short distances; a non-equilibrium or βchaoticβ pattern of differentiation among locations in space; and within locus, within population deviations from the expectations of Hardy-Weinberg equilibrium (HWE). We show that despite having a pelagic larval stage, and a wide distribution across Caribbean coral reefs, this species forms groups of highly related individuals at small spatial scales (metres). These spatially clustered family groups cause the observed deviations from HWE and local population differentiation, a finding that is rarely demonstrated, but could be more common than previously thought
Centerscope
Centerscope, formerly Scope, was published by the Boston University Medical Center "to communicate the concern of the Medical Center for the development and maintenance of improved health care in contemporary society.
Phase 2 study of combination SPI-1620 with docetaxel as second-line advanced biliary tract cancer treatment
Placental syncytiotrophoblast constitutes a major barrier to vertical transmission of Listeria monocytogenes.
Listeria monocytogenes is an important cause of maternal-fetal infections and serves as a model organism to study these important but poorly understood events. L. monocytogenes can infect non-phagocytic cells by two means: direct invasion and cell-to-cell spread. The relative contribution of each method to placental infection is controversial, as is the anatomical site of invasion. Here, we report for the first time the use of first trimester placental organ cultures to quantitatively analyze L. monocytogenes infection of the human placenta. Contrary to previous reports, we found that the syncytiotrophoblast, which constitutes most of the placental surface and is bathed in maternal blood, was highly resistant to L. monocytogenes infection by either internalin-mediated invasion or cell-to-cell spread. Instead, extravillous cytotrophoblasts-which anchor the placenta in the decidua (uterine lining) and abundantly express E-cadherin-served as the primary portal of entry for L. monocytogenes from both extracellular and intracellular compartments. Subsequent bacterial dissemination to the villous stroma, where fetal capillaries are found, was hampered by further cellular and histological barriers. Our study suggests the placenta has evolved multiple mechanisms to resist pathogen infection, especially from maternal blood. These findings provide a novel explanation why almost all placental pathogens have intracellular life cycles: they may need maternal cells to reach the decidua and infect the placenta
Vulnerability and resilience of living marine resources to the Deepwater Horizon oil spill : an overview
Funding for the project was primarily provided by the Gulf of Mexico Research Initiative through several of its research centers.The 2010 Deepwater Horizon (DWH) oil well blowout in the Gulf of Mexico (GoM) was the largest and perhaps most consequential accidental marine oil spill in global history. This paper provides an overview of a Research Topic consisting of four additional papers that: (1) assemble time series data for ecosystem components in regions impacted by the spill, and (2) interpret temporal changes related to the vulnerability of species and ecosystems to DWH and the ensuing resilience to perturbation. Time series abundance data for many taxa pre-date DWH, often by decades, thus allowing an assessment of population- and community-level impacts. We divided the north central GoM into four interconnected βeco-typesβ: the coastal/nearshore, continental shelf, open-ocean pelagic and deep benthic. Key taxa in each eco-type were evaluated for their vulnerability to the circumstances of the DWH spill based on population overlap with oil, susceptibility to oil contamination, and other factors, as well their imputed resilience to population-level impacts, based on life history metrics, ecology and post-spill trajectories. Each taxon was scored as low, medium, or high for 13 vulnerability attributes and 11 resilience attributes to produce overall vulnerability and resilience scores, which themselves were also categorical (i.e., low, medium, or high). The resulting taxon-specific V-R scores provide important guidance on key species to consider and monitor in the event of future spills similar to DWH. Similar analyses may also guide resource allocation to collect baseline data on highly vulnerable taxa or those with low resilience potential in other ecosystems. For some species, even a decade of observation has been insufficient to document recovery given chronic, long-term exposure to DWH oil remaining in all eco-types and because of impacts to the reproductive output of long-lived species. Due to the ongoing threats of deep-water blowouts, continued surveillance of populations affected by DWH is warranted to document long-term recovery or change in system state. The level of population monitoring in the open-ocean and deep benthic eco-types has historically been low and is inconsistent with the continued migration of the oil industry to the ultra-deep (β₯1,500 m) where the majority of leasing, exploration, and production now occurs.Publisher PDFPeer reviewe
Evidence of population-level impacts and resiliency for Gulf of Mexico shelf taxa following the Deepwater Horizon oil spill
The goal of this paper was to review the evidence of population-level impacts of the Deepwater Horizon Oil Spill (DWH) on Gulf of Mexico (GOM) continental shelf taxa, as well as evidence of resiliency following the DWH. There is considerable environmental and biological evidence that GOM shelf taxa were exposed to and suffered direct and indirect impacts of the DWH. Numerous assessments, from mesocosm studies to analysis of biopsied tissue or tissue samples from necropsied animals, revealed a constellation of physiological effects related to DWH impacts on GOM biota, some of which clearly or likely resulted in mortality. While the estimated concentrations of hydrocarbons in shelf waters and sediments were orders of magnitude lower than measured in inshore or deep GOM environments, the level of mortality observed or predicted was substantial for many shelf taxa. In some cases, such as for zooplankton, community shifts following the spill were ephemeral, likely reflecting high rates of population turnover and productivity. In other taxa, such as GOM reef fishes, impacts of the spill are confounded with other stressors, such as fishing mortality or the appearance and rapid population growth of invasive lionfish (Pterois spp.). In yet others, such as cetaceans, modeling efforts to predict population-level effects of the DWH made conservative assumptions given the speciesβ protected status, which post-DWH population assessments either failed to detect or population increases were estimated. A persistent theme that emerged was the lack of precise population-level data or assessments prior to the DWH for many taxa, but even when data or assessments did exist, examining evidence of population resiliency was confounded by other stressors impacting GOM biota. Unless efforts are made to increase the resolution of the data or precision of population assessments, difficulties will likely remain in estimating the scale of population-level effects or resiliency in the case of future large-scale environmental catastrophes
Dynamic Imaging of the Effector Immune Response to Listeria Infection In Vivo
Host defense against the intracellular pathogen Listeria monocytogenes (Lm) requires innate and adaptive immunity. Here, we directly imaged immune cell dynamics at Lm foci established by dendritic cells in the subcapsular red pulp (scDC) using intravital microscopy. Blood borne Lm rapidly associated with scDC. Myelomonocytic cells (MMC) swarmed around non-motile scDC forming foci from which blood flow was excluded. The depletion of scDC after foci were established resulted in a 10-fold reduction in viable Lm, while graded depletion of MMC resulted in 30β1000 fold increase in viable Lm in foci with enhanced blood flow. Effector CD8+ [CD8 superscript +] T cells at sites of infection displayed a two-tiered reduction in motility with antigen independent and antigen dependent components, including stable interactions with infected and non-infected scDC. Thus, swarming MMC contribute to control of Lm prior to development of T cell immunity by direct killing and sequestration from blood flow, while scDC appear to promote Lm survival while preferentially interacting with CD8+ [CD8 superscript +] T cells in effector sites.National Institutes of Health (U.S.) (Grant P01AI-071195
The Role of Anorexia in Resistance and Tolerance to Infections in Drosophila
Infections initiate a signaling loop in which sick animals become anorexic, and the resulting change in diet alters the body's ability to fight infections in good and bad ways
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