Aplicación de compuestos GRAS para el control de la pudrición blanda en frutos de Jaca (Artocarpus heterophyllus L.) causado por Rhizopus stolonifer

Jackfruit is affected by various pathogens in the post-harvest stage, among which is Rhizopus stolonifer, the causal agent of soft rot. To control this pathogen, fungicides are used which damage the environment and affect human health. This situation encourages the search for safe alternatives, among which is chitosan, which has fungicidal characteristics and controls the ripening of various fruits. Also, potassium sorbate is a compound that has traditionally been used to preserve food. In this study, chitosan (Chi) and potassium sorbate (PS) was applied to inhibit the development of R. stolonifer. Determining the mycelial growth, spore germination, sporulation, the severity of the disease, as well as the activity of enzymes involved in the defense of the fruit such as peroxidase (POD) and polyphenoloxidase (PPO). Obtaining a 100% reduction in mycelial growth and spore germination with the 1% Chi-1.0% PS combination. Furthermore, soft rot is not evident when the same treatment was applied to jackfruit, inducing the activity of POD and PPO. The application of chitosan combined with potassium sorbate may be a promising alternative against soft rot in jackfruit.El fruto de yaca es afectado por diversos patógenos en la etapa de postcosecha entre los que se encuentra el hongo Rhizopus stolonifer agente causal de la pudrición blanda. Para el control de este patógeno se utilizan fungicidas que dañan el medio ambiente y afectan la salud humana. Esta situación llevaa buscar alternativas seguras entre las que se encuentra el quitosano, con características fungicidas y control dela maduración en diversos frutos. Asimismo, el sorbato de potasio es un compuesto que se ha utilizado tradicionalmente para conservar alimentos. En este estudio se aplicó quitosano (Chi, por sus siglas en inglés) y sorbato de potasio (PS, por sus siglas en inglés) para inhibir el desarrollo de R. stolonifer. Se determinó el crecimiento micelial, germinación de esporas, esporulación, severidad de la enfermedad, así como la actividad de las enzimas involucradas en la defensa del fruto como la peroxidasa (POD) y la polifenoloxidasa (PPO). El resultado es una reducción al 100% del crecimiento micelial y la germinación de esporas con la combinación 1% Chi-1.0%PS, tampoco se manifestó unapudrición blanda cuando se aplicó el mismo tratamiento en la yaca, con la inducción en la actividad de POD y PPO. La aplicación de quitosano combinado con sorbato de potasio puede ser una alternativa prometedora contra la pudrición blanda en frutos de yaca

Sequencing of Non-model Plants for Understanding the Physiological Responses in Plants

From a genomic point of view, plants are complex organisms. Plants adapt to the environment, by developing different physiological and genetic properties, changing their genomic and expression profiles of adaptive factors, as exemplified by polyploidy studies. These characteristics along with the presence of duplicated genes/genomes make sequencing with early low-throughput DNA sequencing technologies in plants a challenging task. With the development of new technologies for molecular analysis, including transcriptome, proteome or microarray profiling, a new perspective in the genomic analysis was open, making possible to programs in species without genomic maps. The opportunity to extend molecular studies from laboratory model scale toward naturally occurring plant populations made it possible to precisely answer the longstanding important ecological and evolutionary questions. Some plant species have unique properties that could help to understand their adaptability to environment, crop production, pest protection or other biological processes. Molecular studies on non-model plants, including algae, mosses, ferns and plants with very specific characteristics are ongoing

Vigilancia e instituciones en México. La agenda pendiente de la privacidad y la protección de datos personales.

Este libro reúne discusiones que vinculan los proyectos de seguridad ciudadana con la agenda internacional de protección de datos personales y privacidad, enfatizando las características y repercusiones que la debilidad institucional supone. De alguna manera, un interés común entre los autores es la contextualización de las principales líneas de debate internacional en torno a la privacidad y a la protección de datos personales y con base en este contexto general, se realiza un ejercicio que pretende esquematizar los ejes principales de la discusión en nuestro país, con la finalidad de ubicar su especificidad en diversos ámbitos y dimensiones.Sin duda, la agenda sobre el tema se encuentra abierta y esta obra busca contribuir a la discusión por medio de distintos casos en los que se analiza la problemática de la privacidad desde diferentes ópticas y por medio de diferentes actores y casos relevantes. Como habrá notado el lector, la privacidad representa no sólo una polémica en el ámbito académico, ya que también nos orienta a replantear la narrativa en torno a la seguridad nacional, el combate al crimen organizado, la atención humanitaria de los migrantes, la eficiencia de la política social e incluso la atención en situaciones de emergencia como desastres naturales. De ahí que este libro exponga distintos enfoques del tema, con ángulos contrastantes en algunos casos, complementarios en otros. Sirvan estas investigaciones para establecer parámetros de una agenda social en construcción: la privacidad en México.Universidad Autónoma del Estado de Méxic

Mortality from gastrointestinal congenital anomalies at 264 hospitals in 74 low-income, middle-income, and high-income countries: a multicentre, international, prospective cohort study

Summary Background Congenital anomalies are the fifth leading cause of mortality in children younger than 5 years globally. Many gastrointestinal congenital anomalies are fatal without timely access to neonatal surgical care, but few studies have been done on these conditions in low-income and middle-income countries (LMICs). We compared outcomes of the seven most common gastrointestinal congenital anomalies in low-income, middle-income, and high-income countries globally, and identified factors associated with mortality. Methods We did a multicentre, international prospective cohort study of patients younger than 16 years, presenting to hospital for the first time with oesophageal atresia, congenital diaphragmatic hernia, intestinal atresia, gastroschisis, exomphalos, anorectal malformation, and Hirschsprung’s disease. Recruitment was of consecutive patients for a minimum of 1 month between October, 2018, and April, 2019. We collected data on patient demographics, clinical status, interventions, and outcomes using the REDCap platform. Patients were followed up for 30 days after primary intervention, or 30 days after admission if they did not receive an intervention. The primary outcome was all-cause, in-hospital mortality for all conditions combined and each condition individually, stratified by country income status. We did a complete case analysis. Findings We included 3849 patients with 3975 study conditions (560 with oesophageal atresia, 448 with congenital diaphragmatic hernia, 681 with intestinal atresia, 453 with gastroschisis, 325 with exomphalos, 991 with anorectal malformation, and 517 with Hirschsprung’s disease) from 264 hospitals (89 in high-income countries, 166 in middleincome countries, and nine in low-income countries) in 74 countries. Of the 3849 patients, 2231 (58·0%) were male. Median gestational age at birth was 38 weeks (IQR 36–39) and median bodyweight at presentation was 2·8 kg (2·3–3·3). Mortality among all patients was 37 (39·8%) of 93 in low-income countries, 583 (20·4%) of 2860 in middle-income countries, and 50 (5·6%) of 896 in high-income countries (p<0·0001 between all country income groups). Gastroschisis had the greatest difference in mortality between country income strata (nine [90·0%] of ten in lowincome countries, 97 [31·9%] of 304 in middle-income countries, and two [1·4%] of 139 in high-income countries; p≤0·0001 between all country income groups). Factors significantly associated with higher mortality for all patients combined included country income status (low-income vs high-income countries, risk ratio 2·78 [95% CI 1·88–4·11], p<0·0001; middle-income vs high-income countries, 2·11 [1·59–2·79], p<0·0001), sepsis at presentation (1·20 [1·04–1·40], p=0·016), higher American Society of Anesthesiologists (ASA) score at primary intervention (ASA 4–5 vs ASA 1–2, 1·82 [1·40–2·35], p<0·0001; ASA 3 vs ASA 1–2, 1·58, [1·30–1·92], p<0·0001]), surgical safety checklist not used (1·39 [1·02–1·90], p=0·035), and ventilation or parenteral nutrition unavailable when needed (ventilation 1·96, [1·41–2·71], p=0·0001; parenteral nutrition 1·35, [1·05–1·74], p=0·018). Administration of parenteral nutrition (0·61, [0·47–0·79], p=0·0002) and use of a peripherally inserted central catheter (0·65 [0·50–0·86], p=0·0024) or percutaneous central line (0·69 [0·48–1·00], p=0·049) were associated with lower mortality. Interpretation Unacceptable differences in mortality exist for gastrointestinal congenital anomalies between lowincome, middle-income, and high-income countries. Improving access to quality neonatal surgical care in LMICs will be vital to achieve Sustainable Development Goal 3.2 of ending preventable deaths in neonates and children younger than 5 years by 2030

Postharvest evaluation of Goldfinger banana (FHIA-01) at different storage temperatures followed by an acclimation time

Introduction. Banana fruit is one of the major staple crops grown in the humid tropical areas of Mexico. In spite of the adequate preharvest performance of Goldfinger banana (FHIA-01) in the field, little information about its postharvest behavior has been generated. We decided to study the ripening behavior and quality of fruit during controlled storage and acclimation time. Materials and methods. Banana fruit of the hybrid Musa acuminata X balbisiana ’FHIA-01 Goldfinger’ were stored at 11, 13, 15 and 20 °C for 21 days and then transferred to 25 ± 2°C for acclimation. Fruit sampling under controlled temperatures was performed after 1, 3 and 5 days and the samples were analyzed for CO2 and ethylene production, weight loss, acidity, pH, firmness, and total soluble solids (TSS). Results and discussion. The physiological response and quality of cv. FHIA-01 were affected by the temperature and period of storage and acclimation. During the storage period at 20 °C banana fruit showed total loss of firmness, high TSS (23 °Brix) and full color development (yellow) after 15 days. Fruit at 11 °C and then transferred to 25 °C had delayed maturity with alteration of the ripening process. Conclusion. In general, the optimum storage temperatures for this banana cultivar were 13 °C and 15 °C for 7 to 14 days

Manejo postcosecha aguacate: Situación actual del manejo poscosecha y de enfermedades fungosas del aguacate ‘Hass’ para exportación en Michoacán

Mexico is the world’s leading producer and exporter of avocado ‘Hass’. Due to its nutritional characteristics in human health, the avocado ‘Hass’ has gained great popularity around the world. The objective of this review was to know the current state of avocado harvest and post-harvest management, as well as to review the infection process of the fungi Colletotrichumspp. and Lasiodiplodia theobromae, which cause the diseases called anthracnose and pedicle rot, respectively. These pathologies manifest themselves during postharvest maturation and storage. Currently, the main means of control of these microorganisms is a complex of the fungicides azoxystrobin + fludioxonil, the application of which is allowed only for exports to the United States of America. In Mexico, only the state of Michoacán can export avocado to the United States of America, but it seeks to increase exports to other international markets and integrate other producing regions, so it is necessary to look for alternatives to control postharvest diseases. Through the knowledge of the intrinsic protection mechanisms of the fruit, the bases could be laid to develop and evaluate new control models.México es el principal productor y exportador mundial de aguacate ‘Hass’. Por sus características nutricionales en la salud humana, el aguacate ‘Hass’ ha ganado gran popularidad alrededor del mundo. El objetivo de esta revisión fue conocer el estado actual del manejo de la cosecha y poscosecha del aguacate, así como, revisar el proceso de infección de los hongos Colletotrichum spp. y Lasiodiplodia theobromae, causantes de las enfermedades llamadas antracnosis y pudrición peduncular, respectivamente. Estas patologías se manifiestan durante la maduración poscosecha y almacenamiento. Actualmente, el principal medio de control de estos microorganismos es un complejo de los fungicidas azoxystrobin + fludioxonil, cuya aplicación se permite sólo para exportaciones a Estados Unidos de América. En México, sólo el estado de Michoacán puede exportar aguacate a los Estados Unidos de América, pero se busca aumentar las exportaciones hacia otros mercados internacionales e integrar otras regiones productoras, por lo que es necesario buscar alternativas de control de enfermedades en poscosecha. Mediante el conocimiento de los mecanismos intrínsecos de protección del fruto se podrían sentar las bases para desarrollar y evaluar nuevos modelos de control

Evaluación in vitro del quitosano y aceites esenciales para el control de dos especies patógenas de Colletotrichum aisladas de aguacate (Persea americana Mill)

The antifungal activity of chitosan, essential oils (cinnamon and eucalyptus) and their combination were evaluated against two pathogens Colletotrichum acutatum and Colletotrichum gloeosporioides isolated from avocado (Persea americana Mill). Chitosan was used at different concentrations (0.1, 0.5 and 1.0%). The concentrations of essential oils evaluated were 0.1, 0.5 and 2.0% (eucalyptus and cinnamon). The effect of the combination of chitosan with the essential oils was evaluated using the most effective concentrations of chitosan (0.1 and 0.5%) and the essential oil with the highest inhibitory effect (2%).The treatments of chitosan and essential oils were partially effective against fungal development by inhibiting mycelial growth in a range of 17 to 21% and 18 to 50%, respectively. The mycelia growth of both strains was totally reduced by the application of chitosan (0.1%) with cinnamon essential oil (2%), however; the combination of chitosan with eucalyptus essential oil was not effective. To our knowledge this research is the first that reports chitosan resistant strains of Colletotrichum isolated from avocado fruits.La actividad antifúngica del quitosano, aceites esenciales (canela y eucalipto) y su combinación fueron evaluadas contra dos patógenos Colletotrichum acutatum y Colletotrichum gloeosporioides aislados de aguacate (Persea americana Mill). Se utilizó quitosano a diferentes concentraciones (0.1, 0.5 y 1.0%). Las concentraciones de aceites esenciales evaluadas fueron 0.1, 0.5 y 2.0% (eucalipto y canela). El efecto de la combinación del quitosano con los aceites esenciales se evaluó utilizando las concentraciones más eficaces del quitosano (0.1 y 0.5%) y el aceite esencial de eucalipto y canela con el mayor efecto inhibitorio (2%). Los tratamientos de quitosano y aceites esenciales cada uno por separado fueron parcialmente efectivos para evitar el desarrollo de las cepas evaluadas, inhibiendo el crecimiento micelial en un rango de 17 a 21% y 18 a 50% respectivamente. Por lo contrario, el crecimiento micelial de ambas cepas fue totalmente reducido mediante la aplicación combinada del quitosano (0.1%) con aceite esencial de canela (2%), sin embargo; la combinación del quitosano con aceite esencial de eucalipto no fue efectiva. Hasta donde sabemos, esta investigación es la primera que informa sobre cepas de Colletotrichum resistentes al quitosano aisladas de frutos de aguacate

Non-Chemical Treatments for the Pre- and Post-Harvest Elicitation of Defense Mechanisms in the Fungi–Avocado Pathosystem

The greatest challenge for the avocado (Persea americana Miller) industry is to maintain the quality of the fruit to meet consumer requirements. Anthracnose is considered the most important disease in this industry, and it is caused by different species of the genus Colletotrichum, although other pathogens can be equally important. The defense mechanisms that fruit naturally uses can be triggered in response to the attack of pathogenic microorganisms and also by the application of exogenous elicitors in the form of GRAS compounds. The elicitors are recognized by receptors called PRRs, which are proteins located on the avocado fruit cell surface that have high affinity and specificity for PAMPs, MAMPs, and DAMPs. The activation of defense-signaling pathways depends on ethylene, salicylic, and jasmonic acids, and it occurs hours or days after PTI activation. These defense mechanisms aim to drive the pathogen to death. The application of essential oils, antagonists, volatile compounds, chitosan and silicon has been documented in vitro and on avocado fruit, showing some of them to have elicitor and fungicidal effects that are reflected in the postharvest quality of the fruit and a lower incidence of diseases. The main focus of these studies has been on anthracnose diseases. This review presents the most relevant advances in the use of natural compounds with antifungal and elicitor effects in plant tissues