347 research outputs found
Molecular structure and free energy landscape for electron transport in the decahaem cytochrome MtrF
Density-Matrix Renormalization-Group Analysis of Quantum Critical Points: I. Quantum Spin Chains
We present a simple method, combining the density-matrix
renormalization-group (DMRG) algorithm with finite-size scaling, which permits
the study of critical behavior in quantum spin chains. Spin moments and
dimerization are induced by boundary conditions at the chain ends and these
exhibit power-law decay at critical points. Results are presented for the
spin-1/2 Heisenberg antiferromagnet; an analytic calculation shows that
logarithmic corrections to scaling can sometimes be avoided. We also examine
the spin-1 chain at the critical point separating the Haldane gap and dimerized
phases. Exponents for the dimer-dimer and the spin-spin correlation functions
are consistent with results obtained from bosonization.Comment: 21 pages, 12 figures, new results and added references, to appear in
PR
Magnetization profiles and NMR spectra of doped Haldane chains at finite temperatures
Open segments of S=1 antiferromagnetic spin chains are studied at finite
temperatures and fields using continuous time Quantum Monte Carlo techniques.
By calculating the resulting magnetization profiles for a large range of chain
lengths with fixed field and temperature we reconstruct the experimentally
measured NMR spectrum of impurity doped YBaNiMgO. For
temperatures above the gap the calculated NMR spectra are in excellent
agreement with the experimental results, confirming the existence of
excitations at the end of open S=1 chain segments. At temperatures below the
gap, neglecting inter chain couplings, we still find well defined peaks in the
calculated NMR spectra corresponding to the chain end excitations. At
low temperatures, inter chain couplings could be important, resulting in a more
complicated phase.Comment: 7 pages, 5 figures, minor correction
Chain-Boundary Excitations in the Haldane Phase of 1D Systems
The chain-boundary excitations occurring in the Haldane phaseof
antiferromagnetic spin chains are investigated. The bilinear-biquadratic
hamiltonian is used to study these excitations as a function of the strength of
the biquadratic term, , between . At the AKLT point,
, we show explicitly that these excitations are localized at the
boundaries of the chain on a length scale equal to the correlation length
, and that the on-site magnetization for the first site is
. Applying the density matrixrenormalization group we show that
the chain-boundaryexcitations remain localized at the boundaries for
. As the two critical points are approached the
size of the objects diverges and their amplitude vanishes.Comment: 4 Pages, 4 eps figures. Uses RevTeX 3.0. Submitted to PR
In Situ Monitoring of Intracellular Glucose and Glutamine in CHO Cell Culture
The development of processes to produce biopharmaceuticals industrially is still largely empirical and relies on optimizing both medium formulation and cell line in a product-specific manner. Current small-scale (well plate-based) process development methods cannot provide sufficient sample volume for analysis, to obtain information on nutrient utilization which can be problematic when processes are scaled to industrial fermenters. We envision a platform where essential metabolites can be monitored non-invasively and in real time in an ultra-low volume assay in order to provide additional information on cellular metabolism in high throughput screens. Towards this end, we have developed a model system of Chinese Hamster Ovary cells stably expressing protein-based biosensors for glucose and glutamine. Herein, we demonstrate that these can accurately reflect changing intracellular metabolite concentrations in vivo during batch and fed-batch culture of CHO cells. The ability to monitor intracellular depletion of essential nutrients in high throughput will allow rapid development of improved bioprocesses
Liquid PTVA: a faster and cheaper alternative for generating multi-copy clones in Pichia pastoris
Youth transitions as ‘wiki-transitions’ in youth policies platforms
The version of a journal article that has been accepted for publication in a journal.
This is an Accepted Manuscript of an article published by Taylor & Francis in European Societies on 22/11/2019 available online: http://www.tandfonline.com/10.1080/14616696.2019.1690158.In recent years, a number of youth-focused online platforms have emerged which, in different ways, seek to support young people across Europe in building pathways to independent adulthood. In this article, we draw on data from Edgeryders, a recent youth policy research project, to reflect on the extent to which online discussion platforms are useful instruments for understanding the challenges youth face in their transitions to independent adulthood across Europe. Noting the collaborative emphasis articulated by both the project designers and participants, we ask how we might make sense of the data – and the meanings conveyed by that data – produced by online projects. We propose the notion of ‘wiki-transitions’ as a means of theorising young people’s use of online space to support their transitions to adulthood
Имитация распределенной обработки информации в вычислительных системах и локальных вычислительных сетях
Предложено использовать для анализа вариантов организации распределенной обработки информации в вычислительных системах и локальных вычислительных сетях вероятностный граф реализации вычислительного процесса с явными связями типа вероятностных сетевых графиков.Запропоновано використовувати для аналізу варіантів організації розподіленої обробки інформації в обчислювальних системах і в локальних обчислювальних мережах імовірнісний граф реалізації обчислювального процесу з явними зв’язками типу імовірнісних сіткових графіків.It іs оffered to use for analyzing variants of organization of distributed information processing in computing systems and local computing networks a probabilistic graph for realizing a computing process with evident relationships of the type probabilistic network diagrams
The Anticancer Plant Triterpenoid, Avicin D, Regulates Glucocorticoid Receptor Signaling: Implications for Cellular Metabolism
Avicins, a family of apoptotic triterpene electrophiles, are known to regulate cellular metabolism and energy homeostasis, by targeting the mitochondria. Having evolved from “ancient hopanoids,” avicins bear a structural resemblance with glucocorticoids (GCs), which are the endogenous regulators of metabolism and energy balance. These structural and functional similarities prompted us to compare the mode of action of avicin D with dexamethasone (Dex), a prototypical GC. Using cold competition assay, we show that Avicin D competes with Dex for binding to the GC receptor (GR), leading to its nuclear translocation. In contrast to Dex, avicin-induced nuclear translocation of GR does not result in transcriptional activation of GC-dependent genes. Instead we observe a decrease in the expression of GC-dependent metabolic proteins such as PEPCK and FASN. However, like Dex, avicin D treatment does induce a transrepressive effect on the pro-inflammatory transcription factor NF-κB. While avicin's ability to inhibit NF-κB and its downstream targets appear to be GR-dependent, its pro-apoptotic effects were independent of GR expression. Using various deletion mutants of GR, we demonstrate the requirement of both the DNA and ligand binding domains of GR in mediating avicin D's transrepressive effects. Modeling of avicin-GR interaction revealed that avicin molecule binds only to the antagonist confirmation of GR. These findings suggest that avicin D has properties of being a selective GR modulator that separates transactivation from transrepression. Since the gene-activating properties of GR are mainly linked to its metabolic effects, and the negative interference with the activity of transcription factors to its anti-inflammatory and immune suppressive effects, the identification of such a dissociated GR ligand could have great potential for therapeutic use
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