Alternative mechanism for bacteriophage adsorption to the motile bacterium Caulobacter crescentus

2D and 3D cryo-electron microscopy, together with adsorption kinetics assays of ϕCb13 and ϕCbK phage-infected Caulobacter crescentus, provides insight into the mechanisms of infection. ϕCb13 and ϕCbK actively interact with the flagellum and subsequently attach to receptors on the cell pole. We present evidence that the first interaction of the phage with the bacterial flagellum takes place through a filament on the phage head. This contact with the flagellum facilitates concentration of phage particles around the receptor (i.e., the pilus portals) on the bacterial cell surface, thereby increasing the likelihood of infection. Phage head filaments have not been well characterized and their function is described here. Phage head filaments may systematically underlie the initial interactions of phages with their hosts in other systems and possibly represent a widespread mechanism of efficient phage propagation

I. Flux and color variations of the quadruply imaged quasar HE 0435-1223

aims: We present VRi photometric observations of the quadruply imaged quasar HE 0435-1223, carried out with the Danish 1.54m telescope at the La Silla Observatory. Our aim was to monitor and study the magnitudes and colors of each lensed component as a function of time. methods: We monitored the object during two seasons (2008 and 2009) in the VRi spectral bands, and reduced the data with two independent techniques: difference imaging and PSF (Point Spread Function) fitting.results: Between these two seasons, our results show an evident decrease in flux by ~0.2-0.4 magnitudes of the four lensed components in the three filters. We also found a significant increase (~0.05-0.015) in their V-R and R-i color indices. conclusions: These flux and color variations are very likely caused by intrinsic variations of the quasar between the observed epochs. Microlensing effects probably also affect the brightest "A" lensed component.Comment: 10 pages, 8 figure

Reverberation Mapping Measurements of Black Hole Masses in Six Local Seyfert Galaxies

We present the final results from a high sampling rate, multi-month, spectrophotometric reverberation mapping campaign undertaken to obtain either new or improved Hbeta reverberation lag measurements for several relatively low-luminosity AGNs. We have reliably measured thetime delay between variations in the continuum and Hbeta emission line in six local Seyfert 1 galaxies. These measurements are used to calculate the mass of the supermassive black hole at the center of each of these AGNs. We place our results in context to the most current calibration of the broad-line region (BLR) R-L relationship, where our results remove outliers and reduce the scatter at the low-luminosity end of this relationship. We also present velocity-resolved Hbeta time delay measurements for our complete sample, though the clearest velocity-resolved kinematic signatures have already been published.Comment: 52 pages (AASTeX: 29 pages of text, 8 tables, 7 figures), accepted for publication in the Astrophysical Journa

Microlens OGLE-2005-BLG-169 Implies Cool Neptune-Like Planets are Common

We detect a Neptune mass-ratio (q~8e-5) planetary companion to the lens star in the extremely high-magnification (A~800) microlensing event OGLE-2005-BLG-169. If the parent is a main-sequence star, it has mass M~0.5 M_sun implying a planet mass of ~13 M_earth and projected separation of ~2.7 AU. When intensely monitored over their peak, high-magnification events similar to OGLE-2005-BLG-169 have nearly complete sensitivity to Neptune mass-ratio planets with projected separations of 0.6 to 1.6 Einstein radii, corresponding to 1.6--4.3 AU in the present case. Only two other such events were monitored well enough to detect Neptunes, and so this detection by itself suggests that Neptune mass-ratio planets are common. Moreover, another Neptune was recently discovered at a similar distance from its parent star in a low-magnification event, which are more common but are individually much less sensitive to planets. Combining the two detections yields 90% upper and lower frequency limits f=0.37^{+0.30}_{-0.21} over just 0.4 decades of planet-star separation. In particular, f>16% at 90% confidence. The parent star hosts no Jupiter-mass companions with projected separations within a factor 5 of that of the detected planet. The lens-source relative proper motion is \mu~7--10 mas/yr, implying that if the lens is sufficiently bright, I<23.8, it will be detectable by HST by 3 years after peak. This would permit a more precise estimate of the lens mass and distance, and so the mass and projected separation of the planet. Analogs of OGLE-2005-BLG-169Lb orbiting nearby stars would be difficult to detect by other methods of planet detection, including radial velocities, transits, or astrometry.Comment: Submitted to ApJ Letters, 9 text pages + 4 figures + 1 tabl

Revealing the Structure of an Accretion Disk Through Energy Dependent X-ray Microlensing

We present results from monitoring observations of the gravitationally lensed quasar RX J1131-1231 performed with the Chandra X-ray Observatory. The X-ray observations were planned with relatively long exposures that allowed a search for energy-dependent microlensing in the soft (0.2-2 keV) and hard (2-10 keV) light curves of the images of RX J1131-1231. We detect significant microlensing in the X-ray light-curves of images A and D, and energy-dependent microlensing of image D. The magnification of the soft band appears to be larger than that in the hard band by a factor of ~ 1.3 when image D becomes more magnified. This can be explained by the difference between a compact, softer-spectrum corona that is producing a more extended, harder spectrum reflection component off the disk. This is supported by the evolution of the fluorescent iron line in image D over three consecutive time-averaged phases of the light curve. In the first period, a Fe line at E = 6.36(-0.16,+0.13) keV is detected (at > 99% confidence). In the second period, two Fe lines are detected, one at E = 5.47(-0.08,+0.06) keV (detected at > 99% confidence) and another at E = 6.02(-0.07,+0.09) keV (marginally detected at > 90% confidence), and in the third period, a broadened Fe line at 6.42(-0.15,+0.19) keV is detected (at > 99% confidence). This evolution of the Fe line profile during the microlensing event is consistent with the line distortion expected when a caustic passes over the inner disk where the shape of the fluorescent Fe line is distorted by General Relativistic and Doppler effects.Comment: 20 pages, includes 10 figures, submitted to Ap

A Revised Broad-Line Region Radius and Black Hole Mass for the Narrow-Line Seyfert 1 NGC 4051

We present the first results from a high sampling rate, multi-month reverberation mapping campaign undertaken primarily at MDM Observatory with supporting observations from telescopes around the world. The primary goal of this campaign was to obtain either new or improved Hbeta reverberation lag measurements for several relatively low luminosity AGNs. We feature results for NGC 4051 here because, until now, this object has been a significant outlier from AGN scaling relationships, e.g., it was previously a ~2-3sigma outlier on the relationship between the broad-line region (BLR) radius and the optical continuum luminosity - the R_BLR-L relationship. Our new measurements of the lag time between variations in the continuum and Hbeta emission line made from spectroscopic monitoring of NGC 4051 lead to a measured BLR radius of R_BLR = 1.87 (+0.54 -0.50) light days and black hole mass of M_BH = 1.73 (+0.55 -0.52) x 10^6 M_sun. This radius is consistent with that expected from the R_BLR-L relationship, based on the present luminosity of NGC 4051 and the most current calibration of the relation by Bentz et al. (2009a). We also present a preliminary look at velocity-resolved Hbeta light curves and time delay measurements, although we are unable to reconstruct an unambiguous velocity-resolved reverberation signal.Comment: 38 pages, 7 figures, accepted for publication in ApJ, changes from v1 reflect suggestions from anonymous refere

Selenoprotein P Influences Colitis-Induced Tumorigenesis by Mediating Stemness and Oxidative Damage.

Patients with inflammatory bowel disease are at increased risk for colon cancer due to augmented oxidative stress. These patients also have compromised antioxidant defenses as the result of nutritional deficiencies. The micronutrient selenium is essential for selenoprotein production and is transported from the liver to target tissues via selenoprotein P (SEPP1). Target tissues also produce SEPP1, which is thought to possess an endogenous antioxidant function. Here, we have shown that mice with Sepp1 haploinsufficiency or mutations that disrupt either the selenium transport or the enzymatic domain of SEPP1 exhibit increased colitis-associated carcinogenesis as the result of increased genomic instability and promotion of a protumorigenic microenvironment. Reduced SEPP1 function markedly increased M2-polarized macrophages, indicating a role for SEPP1 in macrophage polarization and immune function. Furthermore, compared with partial loss, complete loss of SEPP1 substantially reduced tumor burden, in part due to increased apoptosis. Using intestinal organoid cultures, we found that, compared with those from WT animals, Sepp1-null cultures display increased stem cell characteristics that are coupled with increased ROS production, DNA damage, proliferation, decreased cell survival, and modulation of WNT signaling in response to H2O2-mediated oxidative stress. Together, these data demonstrate that SEPP1 influences inflammatory tumorigenesis by affecting genomic stability, the inflammatory microenvironment, and epithelial stem cell functions

Detection of Extrasolar Planets by Gravitational Microlensing

Gravitational microlensing provides a unique window on the properties and prevalence of extrasolar planetary systems because of its ability to find low-mass planets at separations of a few AU. The early evidence from microlensing indicates that the most common type of exoplanet yet detected are the so-called "super-Earth" planets of ~10 Earth-masses at a separation of a few AU from their host stars. The detection of two such planets indicates that roughly one third of stars have such planets in the separation range 1.5-4 AU, which is about an order of magnitude larger than the prevalence of gas-giant planets at these separations. We review the basic physics of the microlensing method, and show why this method allows the detection of Earth-mass planets at separations of 2-3 AU with ground-based observations. We explore the conditions that allow the detection of the planetary host stars and allow measurement of planetary orbital parameters. Finally, we show that a low-cost, space-based microlensing survey can provide a comprehensive statistical census of extrasolar planetary systems with sensitivity down to 0.1 Earth-masses at separations ranging from 0.5 AU to infinity.Comment: 43 pages. Very similar to chapter 3 of Exoplanets: Detection, Formation, Properties, Habitability, John Mason, ed. Springer (April 3, 2008

Do Binucleate Cardiomyocytes Have A Role in Myocardial Repair? Insights Using Isolated Rodent Myocytes and Cell Culture

Neonatal and adult cardiomyocytes were isolated from rat hearts. Some of the adult myocytes were cultured to allow for cell dedifferentiation, a phenomenon thought to mimic cell changes that occur in stressed myocardium, with myocytes regressing to a fetal pattern of metabolism and stellate neonatal shape

The Race Between Stars and Quasars in Reionizing Cosmic Hydrogen

The cosmological background of ionizing radiation has been dominated by quasars once the Universe aged by ~2 billion years. At earlier times (redshifts z>3), the observed abundance of bright quasars declined sharply, implying that cosmic hydrogen was reionized by stars instead. Here, we explain the physical origin of the transition between the dominance of stars and quasars as a generic feature of structure formation in the concordance LCDM cosmology. At early times, the fraction of baryons in galaxies grows faster than the maximum (Eddington-limited) growth rate possible for quasars. As a result, quasars were not able to catch up with the rapid early growth of stellar mass in their host galaxies.Comment: 5 pages, 1 figure, Accepted for publication in JCA