Das NLRP3-Inflammasom in experimenteller und humaner Temporallappenepilepsie

Ziel dieser Arbeit ist es, den Zusammenhang zwischen der Aktivierung des NLRP3-Signalwegs und der Epileptogenese zu untersuchen. Die mRNA-Expression von NLRP3 und assoziierten Signalmolekülen im Hippocampus wurde in zwei post-Status-epilepticus (SE)-Mausmodellen für pharmakoresistente Temporallappenepilepsie (TLE) – dem systemischen Pilocarpin-Modell und dem suprahippocampalen Kainsäure (KA)-Modell – longitudinal analysiert. Zusätzlich wurden immunhistochemische Untersuchungen durchgeführt, um die Migration und Aktivierung von Mikroglia und Astrozyten zu erfassen. An Hippocampus-Biopsiegewebe von Patienten mit Hippocampussklerose (HS; n=78) und Patienten mit läsionsassoziierter chronischer TLE (n=34) wurden Genexpressionsanalysen der NLRP3-Signalkaskade durchgeführt. Die humanen Genexpressionsanalysen zeigten, dass die Expression NLRP3-assoziierter Moleküle im Gehirngewebe von Patienten mit HS oder läsionsassoziierter TLE weitgehend unabhängig vom Ausmaß des neuronalen Zellverlusts und der strukturellen Reorganisation des Hippocampus ist. In beiden murinen Epilepsiemodellen waren alle Schlüsselkomponenten des NLRP3-Signalwegs auf mRNA-Ebene signifikant erhöht. Der zeitliche Verlauf der proinflammatorischen mRNA-Expressionsänderungen war jedoch modellabhängig unterschiedlich. Im Pilocarpin-Modell führte SE zu einer Zunahme NLRP3-assoziierter Signalmoleküle vor allem in der frühen Epileptogenese bis zehn Tage nach SE-Induktion. Im Gegensatz dazu erreichten die Tiere im KA-Modell die höchsten mRNA-Werte in der chronischen Phase. Die Migration und Aktivierung von Mikroglia und Astrozyten folgte einem ähnlichen, modellabhängigen Zeitverlauf. Diese Studie untersucht die NLRP3-assoziierte Neuroinflammation über einen langen Zeitraum nach induziertem SE und zeigt eine starke Aktivierung des NLRP3-Signalwegs in den beiden gängigen post-SE-Modellen der TLE. Trotz der Annahme, dass die Pathogenese der TLE in beiden Modellen ähnlich ist, wurden deutliche Unterschiede im zeitlichen Verlauf der Anfallsentwicklung, des neuronalen Zelltods und der molekularen Reorganisation festgestellt

Cardiolipin, α-d-glucopyranosyl, and l-lysylcardiolipin from Gram-positive bacteria: FAB MS, monofilm and X-ray powder diffraction studies

AbstractCardiolipin preparations from Streptococcus B, Listeria welshimeri, Staphylococcus aureus, and a glucosyl and lysyl derivative of cardiolipin were analysed for fatty acid composition and fatty acid combinations. Three different fatty acid patterns are described and up to 17 molecular species were identified in Streptococcus B lipids by high resolution FAB MS. The physicochemical properties of these lipids were characterised in the sodium salt form by monofilm experiments and X-ray powder diffraction. All lipids formed stable monofilms. The minimal space requirement of unsubstituted cardiolipin was dictated by the fatty acid pattern. Substitution with l-lysine led to a decrease of the molecular area, substitution with d-glucopyranosyl to an increase. On self assembly at 100% relative humidity, all preparations adopted lamellar structures. They showed a high degree of order, in spite of the heterogeneous fatty acid compositions and numerous fatty acid combinations. The repeat distances in lamellar fluid phase varied between 4.99 and 5.52 nm, the bilayer thickness between 3.70 and 4.46 nm. Surprising were the low values of sorbed water per molecule of the glucosyl and lysyl derivatives which were 58 and 60%, compared with those of the respective cardiolipin. When Na+ was replaced as counterion by Ba2+, the bilayer structure was retained, but the lipids were in the lamellar gel phase and the fatty acids were tilted between 32 and 53° away from the bilayer normal. Wide angle X-ray diffraction studies and electron density profiles are also reported. Particular properties of glucosyl cardiolipin are discussed

Growth behavior of probiotic microorganisms on levan- and inulin-based fructans

Modulation of the gut microbiota by prebiotics is an effective tool to improve host health. Here, the prebiotic properties of inulin- and levan-based fructans were investigated using microorganisms obtained from strain collections and isolated from probiotic pharmaceuticals and yogurts. Utilizing the recently characterized endo-levanase LevB2286, levan-fructooligosaccharides (L-FOS) were produced and applied for dedicated growth studies. Real-time growth experiments in 48-well format revealed that 8 out of 17 strains isolated from probiotic products or yogurts responded to prebiotic treatment. Lactobacillus paracasei strains, several bifidobacteria, and a Saccharomyces cerevisiae isolate metabolized supplied fructans efficiently. Overall inulin-FOS were consumed more rapidly than L-FOS. However, this effect may be attributed to the lower average degree of polymerization and the presence of GFn-FOS in the I-FOS preparation used. Growth experiments with fractionated L-FOS provided valuable insights into the influence of the degree of polymerization on fermentability by probiotic bacteria

RAB5A and TRAPPC6B are novel targets for Shiga toxin 2a inactivation in kidney epithelial cells

The cardinal virulence factor of human-pathogenic enterohaemorrhagic Escherichia coli (EHEC) is Shiga toxin (Stx), which causes severe extraintestinal complications including kidney failure by damaging renal endothelial cells. In EHEC pathogenesis, the disturbance of the kidney epithelium by Stx becomes increasingly recognised, but how this exactly occurs is unknown. To explore this molecularly, we investigated the Stx receptor content and transcriptomic profile of two human renal epithelial cell lines: highly Stx-sensitive ACHN cells and largely Stx-insensitive Caki-2 cells. Though both lines exhibited the Stx receptor globotriaosylceramide, RNAseq revealed strikingly different transcriptomic responses to an Stx challenge. Using RNAi to silence factors involved in ACHN cells’ Stx response, the greatest protection occurred when silencing RAB5A and TRAPPC6B, two host factors that we newly link to Stx trafficking. Silencing these factors alongside YKT6 fully prevented the cytotoxic Stx effect. Overall, our approach reveals novel subcellular targets for potential therapies against Stx-mediated kidney failure.publishedVersio

Physico-chemical characteristics and primary structure of an affinity-purified α-D-galactose-specific, jacalin-related lectin from the latex of mulberry (Morus indica)

An α-D-galactose specific lectin belonging to the family of jacalin-related lectins (JRL) has been purified by affinity chromatography on cross-linked guar-gum. Mass spectrometric data revealed that the protein harbors two chains like all the members of galactose-specific jacalin-related lectins (gJRL). De novo sequencing of proteolytic peptides demonstrated that the heavier chain consists of 133 amino acids and the lighter chain comprises of 21 or 24 amino acids. The heavier chain contains one N-glycosylation site (Asn47) occupied with either pauci-mannose type [GlcNAc2(Fuc)Man3(Xyl)] or complex type [GlcNAc2(Fuc)Man3(Xyl)GlcNAc(Fuc)Gal] N-glycans. Circular dichroism spectroscopy indicated that the secondary structure of the lectin is predominantly made up of β-sheets, and differential scanning calorimetry revealed a thermal denaturation temperature of 77.6 °C. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) cell viability assays on MCF-7 and MDCK cells showed that the lectin is highly cytotoxic towards both cell lines when dosed at micromolar concentrations, suggesting that it may play a role in the defense mechanism of the plant

A New Mint1 Isoform, but Not the Conventional Mint1, Interacts with the Small GTPase Rab6

Small GTPases of the Rab family are important regulators of a large variety of different cellular functions such as membrane organization and vesicle trafficking. They have been shown to play a role in several human diseases. One prominent member, Rab6, is thought to be involved in the development of Alzheimer’s Disease, the most prevalent mental disorder worldwide. Previous studies have shown that Rab6 impairs the processing of the amyloid precursor protein (APP), which is cleaved to β-amyloid in brains of patients suffering from Alzheimer’s Disease. Additionally, all three members of the Mint adaptor family are implied to participate in the amyloidogenic pathway. Here, we report the identification of a new Mint1 isoform in a yeast two-hybrid screening, Mint1 826, which lacks an eleven amino acid (aa) sequence in the conserved C-terminal region. Mint1 826, but not the conventional Mint1, interacts with Rab6 via the PTB domain. This interaction is nucleotide-dependent, Rab6-specific and influences the subcellular localization of Mint1 826. We were able to detect and sequence a corresponding proteolytic peptide derived from cellular Mint1 826 by mass spectrometry proving the absence of aa 495–505 and could show that the deletion does not influence the ability of this adaptor protein to interact with APP. Taking into account that APP interacts and co-localizes with Mint1 826 and is transported in Rab6 positive vesicles, our data suggest that Mint1 826 bridges APP to the small GTPase at distinct cellular sorting points, establishing Mint1 826 as an important player in regulation of APP trafficking and processing

Valid Presumption of Shiga Toxin-Mediated Damage of Developing Erythrocytes in EHEC-Associated Hemolytic Uremic Syndrome

The global emergence of clinical diseases caused by enterohemorrhagic Escherichia coli (EHEC) is an issue of great concern. EHEC release Shiga toxins (Stxs) as their key virulence factors, and investigations on the cell-damaging mechanisms toward target cells are inevitable for the development of novel mitigation strategies. Stx-mediated hemolytic uremic syndrome (HUS), characterized by the triad of microangiopathic hemolytic anemia, thrombocytopenia, and acute renal injury, is the most severe outcome of an EHEC infection. Hemolytic anemia during HUS is defined as the loss of erythrocytes by mechanical disruption when passing through narrowed microvessels. The formation of thrombi in the microvasculature is considered an indirect effect of Stx-mediated injury mainly of the renal microvascular endothelial cells, resulting in obstructions of vessels. In this review, we summarize and discuss recent data providing evidence that HUS-associated hemolytic anemia may arise not only from intravascular rupture of erythrocytes, but also from the extravascular impairment of erythropoiesis, the development of red blood cells in the bone marrow, via direct Stx-mediated damage of maturing erythrocytes, leading to “non-hemolytic” anemia

Enterohemorrhagic <i>Escherichia coli</i> and a Fresh View on Shiga Toxin-Binding Glycosphingolipids of Primary Human Kidney and Colon Epithelial Cells and Their Toxin Susceptibility

Enterohemorrhagic Escherichia coli (EHEC) are the human pathogenic subset of Shiga toxin (Stx)-producing E. coli (STEC). EHEC are responsible for severe colon infections associated with life-threatening extraintestinal complications such as the hemolytic-uremic syndrome (HUS) and neurological disturbances. Endothelial cells in various human organs are renowned targets of Stx, whereas the role of epithelial cells of colon and kidneys in the infection process has been and is still a matter of debate. This review shortly addresses the clinical impact of EHEC infections, novel aspects of vesicular package of Stx in the intestine and the blood stream as well as Stx-mediated extraintestinal complications and therapeutic options. Here follows a compilation of the Stx-binding glycosphingolipids (GSLs), globotriaosylceramide (Gb3Cer) and globotetraosylceramide (Gb4Cer) and their various lipoforms present in primary human kidney and colon epithelial cells and their distribution in lipid raft-analog membrane preparations. The last issues are the high and extremely low susceptibility of primary renal and colonic epithelial cells, respectively, suggesting a large resilience of the intestinal epithelium against the human-pathogenic Stx1a- and Stx2a-subtypes due to the low content of the high-affinity Stx-receptor Gb3Cer in colon epithelial cells. The review closes with a brief outlook on future challenges of Stx research