Research and Write at the Library

This is a poster presented at the Southeastern Writing Center Conference in February 2019. The poster describes an event, the Research and Write workshop, that is a collaboration between the writing center and the library at Coastal Carolina University

Herbicide-Resistant Weed Seeds Contaminate Grain Sown in the Western Australian Grainbelt

Preventing the introduction of weeds into the farming system through sowing of clean seeds is an essential component of weed management. The weed seed contamination of cleaned grain and herbicide resistance levels of the recovered weed seeds were examined in a study conducted across 74 farms in the Western Australian grainbelt. Most farmers grew and conserved their own crop seed. The majority of cleaned samples had some level of seed contamination from 11 foreign weed and volunteer crop species, with an average of 62 seeds 10 kg−1 grain, substantially higher than the 28 seeds 10 kg−1 grain expected by farmers. The most common weed contaminants across all samples were rigid ryegrass, wild radish, brome, and wild oat.When categorized by crop type, rigid ryegrass was the most frequent contaminant of cereal crops (barley and wheat), however wild radish was the most frequent contaminant of lupin crops. Uncleaned crop seed samples had almost 25 times more contamination than cleaned crop seed. Herbicide resistance was highly prevalent within rigid ryegrass populations recovered from cleaned grain except for glyphosate, which controlled all populations tested. Some resistance was also found in wild radish and wild oat populations; however, brome was susceptible to fluazifop. This study has shown that farmers are unknowingly introducing weed seeds into their farming systems during crop seeding, many of which have herbicide resistance

JWST Pathfinder Telescope Integration

The James Webb Space Telescope (JWST) is a 6.5m, segmented, IR telescope that will explore the first light of the universe after the big bang. In 2014, a major risk reduction effort related to the Alignment, Integration, and Test (AI&T) of the segmented telescope was completed. The Pathfinder telescope includes two Primary Mirror Segment Assemblies (PMSA's) and the Secondary Mirror Assembly (SMA) onto a flight-like composite telescope backplane. This pathfinder allowed the JWST team to assess the alignment process and to better understand the various error sources that need to be accommodated in the flight build. The successful completion of the Pathfinder Telescope provides a final integration roadmap for the flight operations that will start in August 2015

Characterization of basal and lipopolysaccharide-induced microRNA expression in equine peripheral blood mononuclear cells using Next-Generation Sequencing

The innate immune response to lipopolysaccharide contributes substantially to the morbidity and mortality of gram-negative sepsis. Horses and humans share an exquisite sensitivity to lipopolysaccharide and thus the horse may provide valuable comparative insights into this aspect of the inflammatory response. MicroRNAs, small non-coding RNA molecules acting as post-transcriptional regulators of gene expression, have key roles in toll-like receptor signaling regulation but have not been studied in this context in horses. The central hypothesis of this study was that lipopolysaccharide induces differential microRNA expression in equine peripheral blood mononuclear cells in a manner comparable to humans. Illumina Next Generation Sequencing was used to characterize the basal microRNA transcriptome in isolated peripheral blood mononuclear cells from healthy adult horses, and to evaluate LPS-induced changes in microRNA expression in cells cultured for up to four hours. Selected expression changes were validated using quantitative reverse-transcriptase PCR. Only miR-155 was significantly upregulated by LPS, changing in parallel with supernatant tumor necrosis factor-α concentration. Eight additional microRNAs, including miR-146a and miR-146b, showed significant expression change with time in culture without a clear LPS effect. Target predictions indicated a number of potential immunity-associated targets for miR-155 in the horse, including SOCS1, TAB2 and elements of the PI3K signaling pathway, suggesting that it is likely to influence the acute inflammatory response to LPS. Gene alignment showed extensive conservation of the miR-155 precursor gene and associated promoter regions between horses and humans. The basal and LPS-stimulated microRNA expression pattern characterized here were similar to those described in human leukocytes. As well as providing a resource for further research into the roles of microRNAs in immune responses in horses, this will facilitate inter-species comparative study of the role of microRNAs in the inflammatory cascade during endotoxemia and sepsis

Prevalence of Latent Equid Herpesvirus Type 1 in Submandibular Lymph Nodes of Horses in Virginia

Equine Herpesvirus type 1 (EHV-1) typically causes mild respiratory disease, but it can also cause late-term abortion, neonatal foal death and neurologic disease. Once a horse is infected, the virus concentrates to local lymphoid tissue, where it becomes latent. The virus can be reactivated during times of stress, which can lead to the initiation of devastating outbreaks. Understanding the carriage rate of latent EHV-1 in different geographic regions is essential for managing the disease. The objective of the current study was to estimate the prevalence of latent EHV-1 and compare the frequency of each variant in the submandibular lymph nodes of horses in Virginia. Sixty-three submandibular lymph nodes were collected post-partem from horses submitted to regional labs for necropsy, and qPCR was performed. All samples were negative for the gB gene of EHV-1. The results demonstrated a low apparent prevalence of latent EHV-1 DNA in submandibular lymph nodes in this population of horses in Virginia. Despite this, the mainstay for outbreak prevention and mitigation continues to focus on minimizing risks and using appropriate and diligent biosecurity