Relatório de Estágio da Prática de Ensino Supervisionada

Deste trabalho consta a prática desenvolvida no estágio realizado de acordo com o regulamento da Prática de Ensino Supervisionada (PES) do Mestrado em Ensino do 1º e 2º Ciclo que confere habilitação profissional para a docência no 1º Ciclo e nas disciplinas de Matemática, Língua Portuguesa, Ciências da Natureza, História e Geografia de Portugal do 2º Ciclo do Ensino Básico. O objetivo deste relatório é refletir o percurso de formação, a atitude crítica e reflexiva em relação aos desafios, processos e desempenhos do quotidiano profissional experienciado, sendo evidenciadas as dificuldades sentidas e as estratégias utilizadas para ultrapassar os obstáculos. Será, também, apresentada uma investigação sobre a inclusão que designamos de “As Atitudes dos Pares Face à Inclusão de Crianças com Necessidades Educativas Especiais”. A inclusão de crianças com Necessidades Educativas Especiais (NEE) nas nossas escolas é um processo que, para além de educativo e pedagógico, se pretende que seja social e emocional, isto é, que conduza a um clima educativo de sucesso para todos. Os pares são neste processo um elemento chave para a criação de um ambiente inclusivo de sucesso, que une a parte educativa à parte do desenvolvimento afetivo e social. Por este motivo, este trabalho tem como principal objetivo conhecer o modo como a criança com NEE é aceite pelos seus pares numa escola inclusiva do 1º Ciclo de Sátão. O instrumento escolhido para a recolha de dados foi o Inquérito por Questionário, com questões abertas e fechadas, que foram respondidas por escrito. A nossa amostra, contou com 54 alunos, pertencentes a quatro turmas onde estavam incluídas crianças com NEE. A análise dos dados permitiu-nos deduzir que os pares aceitam a inclusão das crianças com NEE, no entanto, esta aceitação não é consensual em todas as áreas indispensáveis a uma inclusão de sucesso. Pese embora a idade das crianças envolvidas, acreditamos que este trabalho abre portas à reflexão em torno da necessidade de se fomentar, desde muito cedo na vida das crianças, valores como a amizade e a igualdade

Riscos da manipulação de citotóxicos

Os medicamentos citotóxicos têm como função a inibição do crescimento e/ou processos vitais das células tumorais contudo, estes não são selectivos, não atingindo apenas as células afectadas. Assim, e acrescido ao facto de sabermos que estes são também carcinogénicos, mutagénicos e teratogénicos para quem os manipula, desenvolvemos este estudo com o objectivo de percebermos o risco que estes correm. Para tal, realizamos um estudo descritivo e transversal, onde o instrumento de recolha de dados foi um inquérito, reunindo assim, uma amostra de 35 profissionais de três hospitais diferentes. Os dados obtidos permitem-nos concluir que todos os manipuladores de citotóxicos presentes na amostra, conhecem os efeitos adversos advindos da manipulação, o que significa que todos têm a percepção do risco que correm. Com este estudo, pretende-se alertar os profissionais de saúde que contactam directamente com esta realidade para a execução de boas práticas, a fim de reduzir o risco a que estão expostos

Changes in lignin content of Hypericum perforatum cells after Colletotrichum gloeosporioides elicitation

Fundação para a Ciência e a Tecnologia (FCT) - POCTI/AGR/40283/200

An Insight into the Release Kinetics

The authors acknowledge the financial support from Fundação para a Ciência e a Tecnologia (FCT-MEC), Portugal, through the dedicated project (PTDC/EDM-EDM/30828/2017) (BeLive) Publisher Copyright: © 2023 by the authors.This work explores the unique features of magnetic-responsive hydrogels to obtain liposomal hydrogel delivery platforms capable of precise magnetically modulated drug release based on the mechanical responses of these hydrogels when exposed to an external magnetic field. Magnetic-responsive liposomal hydrogel delivery systems were prepared by encapsulation of 1,2-dipalmitoyl-sn-glycero-3-phosphocoline (DPPC) multilayered vesicles (MLVs) loaded with ferulic acid (FA), i.e., DPPC:FA liposomes, into gelatin hydrogel membranes containing dispersed iron oxide nanoparticles (MNPs), i.e., magnetic-responsive gelatin. The FA release mechanisms and kinetics from magnetic-responsive liposomal gelatin were studied and compared with those obtained with conventional drug delivery systems, e.g., free liposomal suspensions and hydrogel matrices, to access the effect of liposome entrapment and magnetic field on FA delivery. FA release from liposomal gelatin membranes was well described by the Korsmeyer–Peppas model, indicating that FA release occurred under a controlled diffusional regime, with or without magnetic stimulation. DPPC:FA liposomal gelatin systems provided smoother controlled FA release, relative to that obtained with the liposome suspensions and with the hydrogel platforms, suggesting the promising application of liposomal hydrogel systems in longer-term therapeutics. The magnetic field, with low intensity (0.08 T), was found to stimulate the FA release from magnetic-responsive liposomal gelatin systems, increasing the release rates while shifting the FA release to a quasi-Fickian mechanism. The magnetic-responsive liposomal hydrogels developed in this work offer the possibility to magnetically activate drug release from these liposomal platforms based on a non-thermal related delivery strategy, paving the way for the development of novel and more efficient applications of MLVs and liposomal delivery systems in biomedicine.publishersversionpublishe

Induction of phenolic compounds in Hypericum perforatum L. cells by colletotrichum gloeosporioides elicitation

Changes in phenolic metabolism after elicitation with Colletotrichum gloeosporioides (CG) has been studied in Hypericum perforatum L. (HP) cell suspension cultures. Soluble phenolics were analysed by HPLC–DAD and HPLC–DAD–MS/MS. HP cultures elicited with the CG elicitor showed a significant increase in xanthone accumulation. Xanthone accumulation increased twelve fold when the cells were primed with methyl-jasmonate (MeJ) or salicylic acid (SA), before elicitation. HP cultures exposed only to MeJ produced a set of flavonoids, the flavones which represent a substantial part (approx. 40%) of the total flavonoids accumulated in these cells. The possible importance of xanthones as a component of defence mechanism of HP against biotic stress is discussed.Fundação para a Ciência e a Tecnologia (FCT

The Action of Polyphenols in Diabetes Mellitus and Alzheimer's Disease: A Common Agent for Overlapping Pathologies

Diabetes Mellitus (DM) and Alzheimer's disease (AD) are two prevalent diseases in modern societies, which are caused mainly by current lifestyle, aging and genetic alterations. It has already been demonstrated that these two diseases are associated, since individuals suffering from DM are prone to develop AD. Conversely, it is also known that individuals with AD are more susceptible to DM, namely type 2 diabetes (T2DM). Therefore, these two pathologies, although completely different in terms of symptomatology, end up sharing several mechanisms at the molecular level, with the most obvious being the increase of oxidative stress and inflammation. Polyphenols are natural compounds widely spread in fruits and vegetables whose dietary intake has been considered inversely proportional to the incidence of DM and AD. So, it is believed that this group of phytochemicals may have preventive and therapeutic potential, not only by reducing the risk and delaying the development of these pathologies, but also by improving brain's metabolic profile and cognitive function. The aim of this review is to understand the extent to which DM and AD are related pathologies, the degree of similarity and the relationship between them, to detail the molecular mechanisms by which polyphenols may exert a protective effect, such as antioxidant and anti-inflammatory effects, and highlight possible advantages of their use as common preventive and therapeutic alternatives.info:eu-repo/semantics/publishedVersio

Let there be light...

“Let there be light...” is a project in the scope of the International Year of Light 2015 (IYL 2015) targeted at children of the first school grade. Basically, it comprises nine recreational - scientific workshops that last for approximately 90 minutes. Each workshop is dedicated to a different topic, namely: bioluminescence, mineral observation, the rainbow, light-shadow contrast, battery production, just to name a few, and is designed, in differ- ent approaches, by a distinct team of scientists familiar to the scientific area focused. The activity starts with the dramatized storytelling of a children’s story related to the scientific subject and performed by the team of the public library expert in this area. This moment takes place in an almost magical environment opening the door to the science topic light-related that would be focused later on. In the third part of the workshop, the children are invited to produce plastic works (e.g. drawings, constructions and models) inspired in what they have learned, and that are to be collected in a public exhibition held at the same institution at the end of the project. In the present work, besides the description of the experience, you can find the critical analysis of the activity and the evaluation of the action by all the actors involved (project team and children/teachers that attended the workshops).info:eu-repo/semantics/publishedVersio

Freeform 3D printing using a continuous viscoelastic supporting matrix

Embedded bio-printing has fostered significant advances toward the fabrication of soft complex tissue-like constructs, by providing a physical support that allows the freeform shape maintenance within the prescribed spatial arrangement, even under gravity force. Current supporting materials still present major drawbacks for up-scaling embedded 3D bio-printing technology towards tissue-like constructs with clinically relevant dimensions. Herein, we report a a cost-effective and widely available supporting material for embedded bio-printing consisting on a continuous pseudo-plastic matrix of xanthan-gum (XG). This natural polisaccharide exhibits peculiar rheological properties that have enabled the rapid generation of complex volumetric 3D constructs with out of plane features. The freedom of design within the three orthogonal axes through the independent and controlled bio-printing process opens new opportunities to produce on demand large arbitrary shapes for personalized medicine. Additionally, we have demonstrated the versatile functionality of XG as a photocurable gel reservoir to engineer perfused cell-laden hydrogel constructs, addressing other practical biomedical applications such as in vitro models and organ-on-chip platforms.publishe

Single-Objective Front Optimization: Application to RF Circuit Design

This paper proposes a new algorithm which promotes well distributed non-dominated fronts in the parameters space when a single- objective function is optimized. This algorithm is based on ǫ-dominance concept and maxmin sorting scheme. Besides that, the paper also presents the results of the algorithm when it is used in the automated synthesis of optimum performance CMOS radiofrequency and microwave binary-weighted differential switched capacitor arrays (RFDSCAs). The genetic synthesis tool optimizes a fitness function which is based on the performance parameter of the RFDSCAs. To validate the proposed design methodology, a CMOS RFDSCA is synthesized, using a 0.25 μm BiCMOS technology.N/

Thought on Food: A Systematic Review of Current Approaches and Challenges for Food Intake Detection

Nowadays, individuals have very stressful lifestyles, affecting their nutritional habits. In the early stages of life, teenagers begin to exhibit bad habits and inadequate nutrition. Likewise, other people with dementia, Alzheimer’s disease, or other conditions may not take food or medicine regularly. Therefore, the ability to monitor could be beneficial for them and for the doctors that can analyze the patterns of eating habits and their correlation with overall health. Many sensors help accurately detect food intake episodes, including electrogastrography, cameras, microphones, and inertial sensors. Accurate detection may provide better control to enable healthy nutrition habits. This paper presents a systematic review of the use of technology for food intake detection, focusing on the different sensors and methodologies used. The search was performed with a Natural Language Processing (NLP) framework that helps screen irrelevant studies while following the PRISMA methodology. It automatically searched and filtered the research studies in different databases, including PubMed, Springer, ACM, IEEE Xplore, MDPI, and Elsevier. Then, the manual analysis selected 30 papers based on the results of the framework for further analysis, which support the interest in using sensors for food intake detection and nutrition assessment. The mainly used sensors are cameras, inertial, and acoustic sensors that handle the recognition of food intake episodes with artificial intelligence techniques. This research identifies the most used sensors and data processing methodologies to detect food intake.info:eu-repo/semantics/publishedVersio