Comunidades de besouros de serapilheira e a sucess?o natural em uma floresta estacional semidec?dua. MG.

Nosso estudo investigou as respostas das comunidades de besouros de serapilheira ao processo de sucess?o florestal e varia??o temporal. O estudo foi conduzido na Esta??o Ecol?gica Tripu?, de julho (2001) a janeiro (2002). Avaliamos riqueza, abund?ncia, composi??o de esp?cies e flutua??o populacional dos grupos mais representativos no estudo. Foram coletados 3513 indiv?duos de 176 morfoesp?cies. Houve diferen?a de riqueza, abund?ncia e composi??o em rela??o ao est?gio sucessional e ?poca da coleta: floresta avan?ada nos meses de maior precipita??o apresentou riqueza e abund?ncia maiores, al?m de composi??o diferenciada. A flutua??o populacional nos grupos representativos pode ser explicada pela precipita??o. Conclu?mos que sucess?o e precipita??o afetam a comunidade de besouros e ? necess?rio conhecimento biol?gico do grupo estudado para coleta de dados confi?veis.Our study investigated the response of litter-dwelling beetle communities to forest successional stage and to temporal variation. The study was conducted in Esta??o Ecol?gica Tripu?, from July (2001) to January (2002). Here we evaluate richness, abundance, species composition and population fluctuation. We collected 3513 individuals of 176 morphospecies. We detected differences of richness, abundance and composition. Forests in the advanced stages of succession during the months with the highest rainfall, have higher richness and abundance. These forest also have own composition. Population fluctuation can be explained by rainfall. We conclude that both forest sucession and rainfall influence the beetle community. Biological knowledge of the group under study is necessary for confidence in data collection

Levantamento soro-epidemiológico de artrite-encefalite caprina em rebanhos caprinos no semiárido Baiano.

Seroepidemiological survey of caprine arthritis encephalitis in goat flocks of semi-arid region in Bahia State, Brazil. Abstract - In order to determine the serological prevalence of CAE in the Microregion of Juazeiro, Bahia, 693 serum samples of goat were collected from 46 farms from different locations in eight counties that make up this region (Campo Alegre de Lourdes, Casa Nova, Curaçá, Juazeiro, Pilão Arcado, Remanso, Sento Sé and Sobradinho). The samples were tested by agar gel immunodifusion (AGID) and the seroprevalence obtained was 0.87% (6/693). The low prevalence obtained was due to production features. The predominant system is the extensive one, with a presence of native, crossbred and mongrel animals, aiming at the production of meat and skin, with low productivity and technification.IX Congresso Brasileiro Buiatria, outubro de 2011, Goiânia, Goiás, Brasil

Lactate and base deficit are predictors of mortality in critically ill patients with cancer

Cancer patients frequently require admission to intensive care unit. However, there are a few data regarding predictive factors for mortality in this group of patients. The aim of this study was to evaluate whether arterial lactate or standard base deficit on admission and after 24 hours can predict mortality for patients with cancer.Journal ArticleSCOPUS: ar.jinfo:eu-repo/semantics/publishe

Inquérito soro-epidemiológico de Brucella ovis em rebanhos ovinos no semiárido Baiano.

SEROEPIDEMIOLOGICAL SURVEY FOR Brucella ovis INFECTION IN SHEEP FLOCKS OF SEMI-ARID REGION IN BAHIA STATE, BRAZIL. ABSTRACT - In order to analyze the occurrence of antibodies to Brucella ovis in sheep of properties located in the microregion of Juazeiro, Bahia State, Brazil, a survey was conducted in sheep herds in the eight cities that make up the microregion (Juazeiro, Casa Nova, Remanso, Campo Alegre de Lourdes, Pilão Arcado, Sento Sé, Sobradinho and Curaçá). The agar gel immunodifusion test (AGID) was used to examine 694 serum samples of 58 herds. Antibodies to B. ovis were found in 5 (0.72%) of the investigated animals and five properties (8.62%) showed positive animals. It is believed the low number of positive sheep found in this survey is related to the production systems features. The microregion of Juazeiro is characterized by a semi-arid climate and the predominant system is the extensive one, with a presence of native, crossbred and mongrel animals, aiming at the production of meat and skin, with low productivity and technification.IX Congresso Brasileiro Buiatria, outubro 2011, Goiânia, GO, Brasil

Avaliação do emprego de cartões FTA para o transporte do DNA de Pasteurella multocida e pesquisa de genes associados à virulência em cepas isoladas de cólera aviária nos Estados Unidos

Fowl Cholera (FC) is a disease caused by Pasteurella multocida. The severity of this disease is partly caused by virulence factors. Genes encoding fimbriae, capsule, sialidases and proteins for iron metabolism may be related to P. multocida’s ability to infect the host. Besides to examining DNA for the presence of virulence genes, DNA is essential for the diagnostic and FTA cards are an alternative for genetic material transport. The study aims to evaluate the viability of P. multocida DNA transport using the cards and to detect 14 virulence genes in 27 strains isolated from FC cases in the United States by multiplex-PCR. No growth was observed in any of the FTA cards, which was essential to assess the security. Furthermore, DNA detection was possible in 100% of the samples, independent of the storage period (7 to 35 days) and temperature (4°C and 37°C). ptfA, exbd-tonB, hgbA, nanB, oma87, hyaD-hyaC, sodC, hgbB, sodA, nanH and pfhA genes were detected in more than 80% of the samples. FTA cards have proven to be a viable and safe tool for DNA transport of P. multocida. A majority of genes showed a high frequency, which was similar to strains isolated from FC cases.Cólera aviária (CA) é uma doença causada pela bactéria Pasteurella multocida e a severidade dos casos é em parte justificada por fatores de virulência. Genes codificando fímbrias, cápsulas, sialidases, dismutases e proteínas do metabolismo férrico podem ser relacionados à capacidade do agente em infectar o hospedeiro. Além da obtenção do DNA para pesquisa de genes de virulência, o material genético é fundamental para o diagnóstico, e os cartões FTA seriam uma alternativa no transporte de microrganismos. Os objetivos da presente pesquisa foram avaliar a viabilidade do transporte de DNA de P. multocida através dos cartões e detectar 14 genes de virulência em 27 cepas isoladas de CA nos Estados Unidos, por meio de multiplex-PCR. Nenhuma das amostras para análise microbiológica da segurança dos cartões apresentou crescimento. Foi possível a detecção do DNA em 100% das amostras, independentemente do tempo de estocagem (sete a 35 dias) e das temperaturas (4°C e 37°C) avaliadas. Genes ptfA, exbd-tonB, hgbA, nanB, oma87, hyaD-hyaC, sodC, hgbB, sodA, nanH e pfhA foram detectados em mais de 80% das amostras. Os cartões FTA demonstraram ser uma ferramenta viável e segura para o transporte do DNA de P. multocida. A maioria dos genes apresentou uma alta frequência, compatível com isolados de CA

Avaliação do fluxo de seiva em cultivares de soja em três níveis de disponibilidade hídriva no solo.

bitstream/item/71829/1/ID-30960.pd

Demethylation of the Coding Region Triggers the Activation of the Human Testis-Specific PDHA2 Gene in Somatic Tissues

Human PDHA2 is a testis-specific gene that codes for the E1α subunit of Pyruvate Dehydrogenase Complex (PDC), a crucial enzyme system in cell energy metabolism. Since activation of the PDHA2 gene in somatic cells could be a new therapeutic approach for PDC deficiency, we aimed to identify the regulatory mechanisms underlying the human PDHA2 gene expression. Functional deletion studies revealed that the −122 to −6 promoter region is indispensable for basal expression of this TATA-less promoter, and suggested a role of an epigenetic program in the control of PDHA2 gene expression. Indeed, treatment of SH-SY5Y cells with the hypomethylating agent 5-Aza-2′-deoxycytidine (DAC) promoted the reactivation of the PDHA2 gene, by inducing the recruitment of the RNA polymerase II to the proximal promoter region and the consequent increase in PDHA2 mRNA levels. Bisulfite sequencing analysis revealed that DAC treatment induced a significant demethylation of the CpG island II (nucleotides +197 to +460) in PDHA2 coding region, while the promoter region remained highly methylated. Taken together with our previous results that show an in vivo correlation between PDHA2 expression and the demethylation of the CpG island II in testis germ cells, the present results show that internal methylation of the PDHA2 gene plays a part in its repression in somatic cells. In conclusion, our data support the novel finding that methylation of the PDHA2 coding region can inhibit gene transcription. This represents a key mechanism for absence of PDHA2 expression in somatic cells and a target for PDC therapy

Prevalência sorológica da artrite-encefalite caprina em rebanhos caprinos do município de Juazeiro, Bahia, Brasil.

Serologic prevalence of caprine arthritis-encephalitis (cae) in the city of Juazeiro, Bahia, Brazil. This study aims to verify serologic prevalence of caprine arthritis-encephalitis (CAE) in the city of Juazeiro, Bahia, by carrying out an agar gel immunodiffusion proof test (AGID). Furthermore, it attempts to characterize the region's breeding systems. To this end, 150 samples of goat blood serum were analyzed from 10 farms at different localities in Juazeiro. The health information management was recorded in the questionnaire itself. Extensive farming systems and mixed breeds are predominant in the region and animals are raised mainly for meat. The farms also show low productivity and technification. Several diseases were observed, such as caseous lymphadenitis, keratoconjunctivitis, and diarrhea ectoparasitic infestations. Seropositivity was not observed in caprine arthritis-encephalitis, which increases the need for further epidemiological surveys in the region, especially when importing animals for breeding improvement.Edição das palestras e resumos de trabalho apresentados durante o VIII CONGRESSO BRASILEIRO DE BUIATRIA, realizado em Belo Horizonte, outubro de 2009

Study of scattered radiation during fluoroscopy in hip surgery

Objetivo: Medir a intensidade da dose de radiação espalhada em diferentes posições simulando uma intervenção cirúrgica no quadril. Materiais e Métodos: Simulou-se uma intervenção cirúrgica no quadril com apoio da fluoroscopia para estudar a distribuição da radiação espalhada no bloco operatório. Para simular o paciente foi utilizado um simulador antropomórfico de corpo inteiro e para medir a radiação utilizou-se um detector específico para medir raios X. Realizaram-se incidências com um equipamento de raios X tipo arco em C móvel, em modo de escopia contínua, com a ampola a 0° (configuração 1) e a 90° (configuração 2). Os parâmetros operacionais utilizados (voltagem, corrente, tempo de exposição) foram determinados por meio de um estudo estatístico resultante da observação de cirurgias ortopédicas de quadril. Resultados: Em todas as medições observaram-se exposições mais elevadas na configuração 2. Nas medições em função da altura, observaram-se os valores máximos da taxa de dose de 1,167 (± 0,023) µSv/s e 2,278 (± 0,023) µSv/s nas configurações 1 e 2, respectivamente, correspondendo à altura do tórax dos profissionais. No estudo em torno do paciente os valores máximos registraramse na posição ocupada pelo médico cirurgião. Conclusão: Concluiu-se que a exposição à radiação dos profissionais é baixa, podendo ainda ser reduzida mediante o uso de equipamentos de proteção individualObjective: To measure the scattered radiation dose at different positions simulating hip surgery. Materials and Methods: We simulated fluoroscopy-assisted hip surgery in order to study the distribution of scattered radiation in the operating room. To simulate the patient, we used a anthropomorphic whole-body phantom, and we used an X-ray-specific detector to quantify the radiation. Radiographs were obtained with a mobile C-arm X-ray system in continuous scan mode, with the tube at 0° (configuration 1) or 90° (configuration 2). The operating parameters employed (voltage, current, and exposure time) were determined by a statistical analysis based on the observation of orthopedic surgical procedures involving the hip. Results: For all measurements, higher exposures were observed in configuration 2. In the measurements obtained as a function of height, the maximum dose rates observed were 1.167 (± 0.023) µSv/s and 2.278 (± 0.023) µSv/s in configurations 1 and 2, respectively, corresponding to the chest level of health care professionals within the operating room. Proximal to the patient, the maximum values were recorded in the position occupied by the surgeon. Conclusion: We can conclude that, in the scenario under study, health care professionals workers are exposed to low levels of radiation, and that those levels can be reduced through the use of personal protective equipmen