478 research outputs found
Growth, carcass and meat quality of Casertana, Italian Large White and Duroc x (Landrace x Italian Large White) pigs reared outdoors
To compare growth, skeletal development,
carcass traits and meat quality of different
genotypes, 10 Casertana (CT), 10 Italian Large
White (LW) and 10 Duroc x (Landrace x Italian
Large White) (DU) crosses, barrows of 90 days
of age, were allotted to the same outdoor rearing
and feeding conditions. Live weight was
recorded and average daily gain (ADG) was
calculated. At slaughter (330-day-old) dressing
and lean percentages were determined; backfat
thickness and loin eye depth were measured.
Carcasses were dissected into commercial
cuts. Water holding capacity, pH and colour
(45 min and 24 h post-mortem) were measured.
Longissimus lumborum muscle samples
were collected for cholesterol, \u3b1-tochopherol
and intramuscular collagen (IMC) analyses.
CT compared to DU and LW had the lowest
growth rate and skeletal development.
Casertana showed higher backfat thickness,
lower lean cut/fatty cut ratio and less lean
meat (P<0.05). Loin eye depth differed among
genotypes with LW>DU>CT (P<0.05). CT
showed higher red colour of the meat than DU
and LW (P<0.05). CT compared to LW had the
highest hydroxylysylpiridinoline (HLP)
crosslink concentration and HLP/IMC ratio,
and a lower IMC amount (P<0.05). Casertana
pigs produced meat that could be tougher than
that from the improved breed, but more acceptable
from the technological point of view.
At eleven months of age bone weight, length
and diameter were clearly genetic type-related;
differently, the bone maturit
Acromion Clavicular Joint Reconstruction with LARS Ligament in Acute Dislocation
Background: The acromion clavicular joint dislocations are common injuries of the shoulder. The severity is dependent upon the degree of ligamentous injury. Surgical treatment is typically performed in higher grade acromioclavicular separation with several static and dynamic operative procedures with or without primary ligament replacement.
Methods: 47 patients with acute Rockwood type III, IV, and V injuries were treated surgically with LARS reconstruction. The success of technique was evaluated by radiographic outcomes for each patient at every follow-up visit (one,three, 12 months), while to assess pain reduction and clinical evaluation Visual Analogue scale score (VAS) and Constant-Murley score (CMA) was performed, respectively. An One Way Analysis of Variance (Kruskal-Wallis test), a multiple comparison Turket test, or a t-test (Mann-Whitney Rank Sum Test) were used when required.
Results: Follow-up radiographs revealed maintenance of anatomical reduction in 41 patients, and no bone erosions has been identified. In short-term joint functional recovery has been observed. Indeed, after 12 months pain on the VAS-scale in all groups decreased significantly (P < 0.05), and the CMS revealed a significant overall improvement (P < 0.05).
Conclusion: These data demonstrate that the use of the LARS allows to provide stability to the joint and especially to ensure its natural elasticity, relieving pain and improving joint function already one month post-surgery.
Level of evidence: II
A New Technique for Finding Needles in Haystacks: A Geometric Approach to Distinguishing Between a New Source and Random Fluctuations
We propose a new test statistic based on a score process for determining the
statistical significance of a putative signal that may be a small perturbation
to a noisy experimental background. We derive the reference distribution for
this score test statistic; it has an elegant geometrical interpretation as well
as broad applicability. We illustrate the technique in the context of a model
problem from high-energy particle physics. Monte Carlo experimental results
confirm that the score test results in a significantly improved rate of signal
detection.Comment: 5 pages, 4 figure
DPP9 is a novel component of the N-end rule pathway targeting the tyrosine kinase Syk.
The aminopeptidase DPP9 removes dipeptides from N-termini of substrates having a proline or alanine in second position. Although linked to several pathways including cell survival and metabolism, the molecular mechanisms underlying these outcomes are poorly understood. We identified a novel interaction of DPP9 with Filamin A, which recruits DPP9 to Syk, a central kinase in B-cell signalling. Syk signalling can be terminated by degradation, requiring the ubiquitin E3 ligase Cbl. We show that DPP9 cleaves Syk to produce a neo N-terminus with serine in position 1. Pulse-chases combined with mutagenesis studies reveal that Ser1 strongly influences Syk stability. Furthermore, DPP9 silencing reduces Cbl interaction with Syk, suggesting that DPP9 processing is a prerequisite for Syk ubiquitination. Consistently, DPP9 inhibition stabilizes Syk, thereby modulating Syk signalling. Taken together, we demonstrate DPP9 as a negative regulator of Syk and conclude that DPP9 is a novel integral aminopeptidase of the N-end rule pathway
UV/Optical Emission Accompanying Gamma-ray Burst
We discuss the possible simultaneously UV/optical emission accompanying
Gamma-ray bursts (GRBs). We show that as long as the intrinsic spectrum of GRB
can extend to 10 GeV or higher, there is a large amount of relativistic
pairs generated due to the annihilation of the soft rays with
the very energetic photons, which dominates over the electrons/positrons
associated with the fireball, no matter the fireball is highly magnetized or
not (For the highly magnetized fireball, the magnetic field is ordered, the
high linear polarization of the multi-wavelength emission is expected). We find
that these pairs can power an UV flash with
magnitude, and the corresponding optical emission can be up to magnitude. Such bright UV emission can be detected by
the upcoming satellite Swift, planned for launch in early 2004. The behavior of
the optical-UV spectrum () differs significantly from
that of the reverse shock emission (, ), which is a signature of the emission accompanying with GRB. The
mild optical emission can be detected with the ROTSE-IIIa telescope system, if
the response to the GRB alert is fast enough.Comment: 5 pages, no figures. MNRAS in pres
Fifteen shades of grey: combined analysis of genome-wide SNP data in steppe and mediterranean grey cattle sheds new light on the molecular basis of coat color
Coat color is among the most distinctive phenotypes in cattle. Worldwide, several breeds share peculiar coat color features such as the presence of a fawn pigmentation of the calf at birth, turning over time to grey, and sexual dichromatism. The aim of this study was to search for polymorphisms under differential selection by contrasting grey cattle breeds displaying the above phenotype with non-grey cattle breeds, and to identify the underlying genes. Using medium-density SNP array genotype data, a multi-cohort FST-outlier approach was adopted for a total of 60 pair-wise comparisons of the 15 grey with 4 non-grey cattle breeds (Angus, Limousin, Charolais, and Holstein), with the latter selected as representative of solid and piebald phenotypes, respectively. Overall, more than 50 candidate genes were detected; almost all were either directly or indirectly involved in pigmentation, and some of them were already known for their role in phenotypes related with hair graying in mammals. Notably, 17 relevant genes, including SDR16C5, MOS, SDCBP, and NSMAF, were located in a signal on BTA14 convergently observed in all the four considered scenarios. Overall, the key stages of pigmentation (melanocyte development, melanogenesis, and pigment trafficking/transfer) were all represented among the pleiotropic functions of the candidate genes, suggesting the complex nature of the grey phenotype in cattle
Numerical study of anharmonic vibrational decay in amorphous and paracrystalline silicon
The anharmonic decay rates of atomic vibrations in amorphous silicon (a-Si)
and paracrystalline silicon (p-Si), containing small crystalline grains
embedded in a disordered matrix, are calculated using realistic structural
models. The models are 1000-atom four-coordinated networks relaxed to a local
minimum of the Stillinger-Weber interatomic potential. The vibrational decay
rates are calculated numerically by perturbation theory, taking into account
cubic anharmonicity as the perturbation. The vibrational lifetimes for a-Si are
found to be on picosecond time scales, in agreement with the previous
perturbative and classical molecular dynamics calculations on a 216-atom model.
The calculated decay rates for p-Si are similar to those of a-Si. No modes in
p-Si reside entirely on the crystalline cluster, decoupled from the amorphous
matrix. The localized modes with the largest (up to 59%) weight on the cluster
decay primarily to two diffusons. The numerical results are discussed in
relation to a recent suggestion by van der Voort et al. [Phys. Rev. B {\bf 62},
8072 (2000)] that long vibrational relaxation inferred experimentally may be
due to possible crystalline nanostructures in some types of a-Si.Comment: 9 two-column pages, 13 figure
The nature of the short wavelength excitations in vitreous silica: X-Rays Brillouin scattering study
The dynamical structure factor (S(Q,E)) of vitreous silica has been measured
by Inelastic X-ray Scattering varying the exchanged wavevector (Q) at fixed
exchanged energy (E) - an experimental procedure that, contrary to the usual
one at constant Q, provides spectra with much better identified inelastic
features. This allows the first direct evidence of Brillouin peaks in the
S(Q,E) of SiO_2 at energies above the Boson Peak (BP) energy, a finding that
excludes the possibility that the BP marks the transition from propagating to
localised dynamics in glasses.Comment: 4 pages, 3 Postscript figures. To appear in Physical Review Letter
The very early afterglow powered by the ultra-relativistic mildly magnetized outflows
In the Poynting Flux dominated outflow (the initial ratio of the
electromagnetic energy flux to the particle energy flux ) model
for Gamma-ray bursts, nearly half of the internally dissipated magnetic energy
is converted into the prompt ray energy emission and the rest is
converted into the kinetic energy of the outflow. Consequently, at the end of
the ray burst, decreases significantly ( or
even smaller). We numerically investigate the very early reverse shock emission
powered by such mildly magnetized outflows interacting with medium--uniform
interstellar medium (ISM) or stellar wind (WIND). We show that for
and typical parameters of Gamma-ray bursts, both the
ISM-ejecta interaction and the WIND-ejecta interaction can power very strong
optical emission ( magnitude or even brighter).
Similar to the very early afterglow powered by the non-magnetized ejecta
interacting with the external medium, the main difference between the
ISM-ejecta interaction case and the WIND-ejecta interaction case is that,
before the reverse shock crosses the ejecta, the R-band emission flux increases
rapidly for the former, but for the latter it increases only slightly. (The
abstract has been shortened). We suggest that the linear polarization detection
of the early multi-wavelength afterglow is highly needed to see whether the
outflows powering GRBs are magnetized or not.Comment: 8 pages, 3 figures, A&A in pres
BOVITA: a first overview on genome-wide genetic diversity of Italian autochthonous cattle breeds
Analysis of genomic data is increasingly becoming part of the livestock industry and is an invaluable resource for effective management of breeding programs in small populations. The recent availability of genome-wide SNP panels allows providing background information concerning genome structure in domestic animals, opening new perspectives to livestock genetics. BOVITA was established to join local efforts and resources for the genomic characterization of Italian local cattle breeds. Despite the growing diffusion of some cosmopolite specialized breeds, several autochthonous breeds are still bred in Italy. The main aim of the BOVITA is to investigate the genomic structure of Italian local cattle breeds, to provide information on their genetic status that will be useful for the management of the genetic variability, as a contribution to biodiversity conservation and prioritization actions.
A total of about 800 animals (20-32 per breed) belonging to thirty Italian cattle breeds (Agerolese, Bar\ue0-Pustertaler, Burlina, Cabannina, Calvana, Chianina, Cinisara, Garfagnina, Italian Brown, Italian Holstein, Italian Simmental, Marchigiana, Maremmana, Modenese, Modicana, Mucca Pisana, Pezzata Rossa d\u2019Oropa, Piedmontese, Pinzgau, Podolica, Pontremolese, Pustertaler, Reggiana, Rendena, Romagnola, Rossa Siciliana, Sarda, Sardo-Bruna, Sardo-Modicana and Ottonese-Varzese) and two cosmopolitan breeds (Charolaise and Limousine) genotyped with the Illumina BovineSNP50 v2 BeadChip array were collected for the analysis. The genotypes of several breeds were detected in the frame of the project, whereas for some breeds these data are derived by previous studies. The dataset will be analyzed to: study several aspects of population genetic diversity, multi-dimensional scaling plot, population structure, linkage disequilibrium, and runs of homozygosity. In addition, comparative analysis of conserved haplotypes will be conducted to identify genomic segments under selection pressure. Such information also provides important insights into the mechanisms of evolution and is useful for the annotation of significant functional genomics regions. Data analysis will also be useful to select SNPs suitable for parentage test and breed genetic traceability. The analysis of the data will pinpoint the genetic distinctiveness of Italian breeds. Moreover, the obtained results contribute to a better characterization of history and genetic structure of Italian cattle breeds
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