L’utilizzo di un eptapeptide marcato con fluoresceina per l'identificazione della displasia colica, in pazienti affetti da rettocolite ulcerosa: studio pilota

Aim Targeted molecular probes have been used to detect sporadic colonic dysplasia during confocal laser endomicroscopy (CLE) with promising results. This is a feasibility pilot study aiming to assess the potential role of CLE combined with a fluorescent-labeled peptide to stain and detect dysplasia associated with Ulcerative Colitis. Method A phage-derived heptapeptide with predicted high binding affinity for dysplastic tissue, was synthesized and labeled with fluorescein. Eleven lesions with suspected dysplasia at endoscopy were excised from nine patients with long-standing ulcerative colitis. Specimens were sprayed with the peptide and examined by CLE. The CLE images were then compared to the corresponding histological sections. Results At definitive histology, 4 lesions were diagnosed as inflammatory polyps, 6 as dysplastic lesions and one as invasive cancer. In inflammatory polyps, the fluorescence signal came from peri-cryptal spaces and crypt lumen due to passive accumulation of the peptide in these areas. Dysplasia was associated with active binding of the peptide to dysplastic colonocytes. Conclusion Ex vivo staining of ulcerative colitis-associated dysplasia using a fluorescent labeled molecular probe and CLE is feasible. In vivo studies on larger populations are required to evaluate the safety and the effective contribution of molecular probes in cancer surveillance of ulcerative colitis

Recent kinematics of the tectonic plates surrounding the Red Sea and Gulf of Aden

The Red Sea and Gulf of Aden represent two young basins that formed between Africa and Arabia since the early Oligocene, floored by oceanic crust or by transitional and thinned continental crust. While in the easternmost Gulf of Aden the rift–drift transition can be dated chron C6 (~20.1 Ma), here we show that in the Red Sea the first pulse of sea floor spreading occurred during chron C3n.2n (~4.6 Ma) around ~17.1°N (present–day coordinates) and propagated southwards from this location, separating the Danakil microplate from Arabia. It is also shown that sea floor spreading between Arabia and Nubia started later, around chron 2A (~2.58 Ma), and propagated northwards. At present, there is no magnetic evidence for the existence of a linear spreading center in the northern Red Sea at latitudes higher than ~24°N and in the southern Red Sea below ~14.8°N. The present–day plate kinematics of this region can be described with high accuracy by a network of five interacting plates (Nubia, Arabia, Somalia, Sinai, and Danakil) and six triple junctions. For times older than anomaly 2A (~2.58 Ma) and up to anomaly 3, the absence of marine magnetic anomalies between Arabia and Nubia prevents a rigorous kinematic description of the five–plates system. However, there is strong evidence that the unique changes in plate motions during the last five Myrs were a dramatic slowdown at chron C2 (~1.77 Ma) in the spreading or extension rates along the ridge and rift axes, thereby a good representation of the real plate motions can be obtained anyway by backward extension of the oldest Arabia – Nubia and Arabia – Danakil stage rotations determined on the basis of marine magnetic anomalies, respectively C2 – C2A and C2A – C3. The proposed kinematic reconstructions are accompanied by a geodynamic explanation for the genesis of large continent–continent fracture zones at the rift–drift transition and by an analysis of the strain associated with plate motions in Afar, northeastern Egypt, and Sinai

The opening of the Tyrrhenian basin and the Apennine chain formation in the kinematic context of Africa - Europe collision

EGU2020: Sharing Geoscience Online, 4-8 may 2020The Africa Europe collision, which produces the formation of the Alpine arc, in the Mediterranean area is accompanied by passive subduction processes, resulting from the sinking of the remnant Alpine Tethys and the Ionian lithosphere, and from the fragmentation of the Adriatic plate. In this complex deformation, back-arc basins (Alboran, Balearic, Tyrrhenian and Hellenic) and circum - Mediterranean mountain ranges are formed. In this work we focus our attention on the opening of the Tyrrhenian basin and the contemporary formation of the Apennine chain. In order to describe the evolution of the geodynamic processes that guided the formation of the Tyrrhenian basin and the Apennine chain we used the plate kinematics technique. Through careful observation of the regional structures we have divided the area of the Apennine Chain and the Tyrrhenian basin into polygons (crustal blocks or microplates) distinguished on the basis of the direction of the Tyrrhenian extension. The boundary between the polygons has been placed coinciding with the large structures that characterize the Tyrrhenian-Apennine area. The rotation poles of the individual polygons, in the frame of reference of the Sardo-Corso block, are based on the Tyrrhenian extension directions that characterize them. The velocity ratio between the polygons was determined by the slip vector of the structure (plate boundary) that separates them. To determine the rotation time of the polygons we used the stratigraphic records of the syn-rift sequences, while the rotation angle of the polygons is obtained comparing the crustal balance with the speed ratios. Finally, the kinematic framework obtained, included in the global rotation model, allowed us to reconstruct the tectonic evolution of the central Mediterranean during the opening of the Tyrrhenian basin

PV reconfiguration systems: A technical and economic study

Dynamical electrical array reconfiguration strategies for grid-connected PV systems have been proposed as solution to improve energy production due to the mismatch effect of PV plants during partial shading conditions. Strategies are based on the use of dynamic connections between PV panels given by the employment of switches that allow for each panel the series, parallel or exclusion connections, physically changing the electrical connections between the related PV modules, consequentially modifying the layout of the plant. Usually the cost of the dynamic matrix is not taken into account. This novel work evaluates the economic advantages obtained by the use of reconfiguration strategies in PV systems, by taking into consideration the price of energy due to incentives in different European and non-European countries and correlates it with the employment of two types of reconfigurators, with different internal structures. For each of the incentives proposed by the different Countries, the main strength and weakness points of the possible investment are highlighted and critically analyzed. From this analysis, it can be stated that the adoption of reconfiguration systems, in certain cases, can be a very convenient solution

Contributed Review: Review of thermal methods for space charge measurement

The space charge accumulation phenomenon has garnered great interest over the last two decades because of the increased use of direct current in high voltage electrical systems. In this context, a significant relevance has been achieved by the thermal methods, used for solid dielectrics. This paper presents a review of this non-destructive measurement system used for the measurement of space charge. The thermal pulse method, the thermal step method, and the laser intensity modulation method are described. For each configuration, the principle of operation, the thicknesses analyzed, and the spatial resolution are described, reporting also the main related application

A New Southern North Atlantic Isochron Map: Insights Into the Drift of the Iberian Plate Since the Late Cretaceous

This paper presents a new southern North Atlantic plate model from Late Cretaceous to present, with the aim of constraining the kinematics of the Iberian plate during the last 83.5 Myr. This model is presented along with a detailed isochron map generated through the analysis of 3 aeromagnetic tracks and ~400 ship tracks from the National Centers for Environmental Information database. We present a new technique to obtain well‐constrained estimates of the Iberia‐North America plate motions from magnetic anomalies, overcoming the scarcity of large‐offset fracture zones and transform faults. We build an integrated kinematic model for NW Africa, Morocco, Iberia, Europe, and North America, which shows that the deformation is partitioned between Pyrenees and Betic‐Rif orogenic domain during the Late Cretaceous‐Oligocene time interval. In the Eastern Betics domain, the calculated amount of NW Africa‐Iberia convergence is ~80 km between 83.5 and 34 Ma, followed by ~150 km since the Oligocene. The motion of Iberia relative to Europe in the Central Pyrenees is characterized by overall NE directed transpressional motion during the Campanian and the Paleocene, followed by NW directed transpressional movement until the Lutetian and overall NNW directed convergence from Bartonian to Chattian. This motion occurs along the axis of the Bay of Biscay from the Santonian–Campanian boundary to the middle Priabonian, subsequently jumping to King's Trough at Anomaly 17 (36.62 Ma)

The Longevity of Fruit Trees in Basilicata (Southern Italy): Implications for Agricultural Biodiversity Conservation

In the Mediterranean basin, agriculture and other forms of human land use have shaped the environment since ancient times. Intensive and extensive agricultural systems managed with a few cultured plant populations of improved varieties are a widespread reality in many Mediterranean countries. Despite this, historical cultural landscapes still exist in interior and less intensively managed rural areas. There, ancient fruit tree varieties have survived modern cultivation systems, preserving a unique genetic heritage. In this study, we mapped and characterized 106 living fruit trees of ancient varieties in the Basilicata region of southern Italy. Tree ages were determined through tree ring measurements and radiocarbon analyses. We uncovered some of the oldest scientifically dated fruit trees in the world. The oldest fruit species were olive (max age 680 +/- 57 years), mulberry (647 +/- 66 years), chestnut (636 +/- 66 years), and pear (467 +/- 89 years). These patriarchs hold a unique genetic resource; their preservation and genetic maintenance through agamic propagation are now promoted by the Lucan Agency for the Development and Innovation in Agriculture (ALSIA). Each tree also represents a hub for biodiversity conservation in agrarian ecosystems: their large architecture and time persistence guarantee ecological niches and micro-habitats suitable for flora and fauna species of conservation significance

Detection of colonic dysplasia in patients with ulcerative colitis using a targeted fluorescent peptide and confocal laser endomicroscopy: A pilot study

Targeted molecular probes have been used to detect sporadic colonic dysplasia during confocal laser endomicroscopy (CLE) with promising results. This is a feasibility pilot study aiming to assess the potential role of CLE combined with a fluorescent-labeled peptide to stain and detect dysplasia associated with Ulcerative Colitis