Decomposition of variance in terms of conditional means

We test against two different sets of data an apparently new approach to the analysis of the variance of a numerical variable which depends on qualitative characters. We suggest that this approach be used to complement other existing techniques to study the interdependence of the variables involved. According to our method the variance is expressed as a sum of orthogonal components, obtained as differences of conditional means, with respect to the qualitative characters. The resulting expression for the variance depends on the ordering in which the characters are considered. We suggest an algorithm which leads to an ordering which is deemed natural. The first set of data concerns the score achieved by a population of students, on an entrance examination, based on a multiple choice test with 30 questions. In this case the qualitative characters are dyadic and correspond to correct or incorrect answer to each question. The second set of data concerns the delay in obtaining the degree for a population of graduates of Italian universities. The variance in this case is analyzed with respect to a set of seven specific qualitative characters of the population studied (gender, previous education, working condition, parent's educational level, field of study, etc.)Comment: 3 figure

A FACIAL ANIMATION FRAMEWORK WITH EMOTIVE/EXPRESSIVE CAPABILITIES

LUCIA is an MPEG-4 facial animation system developed at ISTC-CNR.. It works on standard Facial Animation Parameters and speaks with the Italian version of FESTIVAL TTS. To achieve an emotive/expressive talking head LUCIA was build from real human data physically extracted by ELITE optotracking movement analyzer. LUCIA can copy a real human by reproducing the movements of passive markers positioned on his face and recorded by the ELITE device or can be driven by an emotional XML tagged input text, thus realizing a true audio/visual emotive/expressive synthesis. Synchronization between visual and audio data is very important in order to create the correct WAV and FAP files needed for the animation. LUCIA\u27s voice is based on the ISTC Italian version of FESTIVAL-MBROLA packages, modified by means of an appropriate APML/VSML tagged language. LUCIA is available in two different versions: an open source framework and the "work in progress" WebG

A 3d talking head for mobile devices based on unofficial ios webgl support

In this paper we present the implementation of a WebGL Talking Head for iOS mobile devices (Apple iPhone and iPad). It works on standard MPEG-4 Facial Animation Parameters (FAPs) and speaks with the Italian version of FESTIVAL TTS. It is totally based on true real human data. The 3D kinematics information are used to create lips articulatory model and to drive directly the talking face, generating human facial movements. In the last year we developed the WebGL version of the avatar. WebGL, which is 3D graphic for the web, is currently supported in the major web browsers for desktop computers. No official support has been given for mobile device main platforms yet, although the Firefox beta version enables it on android phones. Starting from iOS 5 WebGL is enabled only for the advertisement library class (which is intended for placing ad-banners in applications). We have been able to use this feature to visualize and animate our WebGL talking head

LUCIA: An open source 3D expressive avatar for multimodal h.m.i.

LUCIA is an MPEG-4 facial animation system developed at ISTC-CNR . It works on standard Facial Animation Parameters and speaks with the Italian version of FESTIVAL TTS. To achieve an emotive/expressive talking head LUCIA was build from real human data physically extracted by ELITE optotracking movement analyzer. LUCIA can copy a real human by reproducing the movements of passive markers positioned on his face and recorded by the ELITE device or can be driven by an emotional XML tagged input text, thus realizing a true audio/visual emotive/expressive synthesis. Synchronization between visual and audio data is very important in order to create the correct WAV and FAP files needed for the animation. LUCIA\u27s voice is based on the ISTC Italian version of FESTIVAL-MBROLA packages, modified by means of an appropriate APML/VSML tagged language. LUCIA is available in two dif-ferent versions: an open source framework and the "work in progress" WebGL

Fish Response to Multiple Anthropogenic Stressors in Mediterranean Coastal Lagoons: A Comparative Study of the Role of Different Management Strategies

Transitional waters are among the most productive ecosystems of the world and their biotic communities show high diversity and complex mechanisms of self-regulation that provide valuable ecosystem services and societal goods and benefits. In this work a comparison of the fish assemblages of three non-tidal Mediterranean coastal lagoons is carried out in order to evaluate the impacts of alternative management strategies. The anthropogenic pressures acting on the lagoons were quantified by means of categorical indicators, while the characteristics of the fish assemblages were summarized in multi-metric indices (MMIs). Two MMIs were developed using data collected with a beach seine net and with fyke nets, following an empirical approach that selects, from a pool of 73 metrics, the combination that maximizes the MMI/pressure relationship. The two MMIs include four metrics each, most of which are based on feeding mode functional guilds and habitat use functional guilds, and they are sensitive to anthropogenic pressures. The human activities directly or indirectly affecting water quality are the ones that most influence the fish assemblage, while the presence of artisanal fisheries, a typical and relevant resource use in these lagoons, seems to play a beneficial role. Lagoon fisheries management relies on the maintenance of infrastructures that guarantee the hydraulic functioning of the lagoon, thus ensuring exchanges with the adjacent coastal sea, and therefore indirectly contributing to the habitat quality

Sweeter and stronger: Enhancing sweetness and stability of the single chain monellin MNEI through molecular design

Sweet proteins are a family of proteins with no structure or sequence homology, able to elicit a sweet sensation in humans through their interaction with the dimeric T1R2-T1R3 sweet receptor. In particular, monellin and its single chain derivative (MNEI) are among the sweetest proteins known to men. Starting from a careful analysis of the surface electrostatic potentials, we have designed new mutants of MNEI with enhanced sweetness. Then, we have included in the most promising variant the stabilising mutation E23Q, obtaining a construct with enhanced performances, which combines extreme sweetness to high, pH-independent, thermal stability. The resulting mutant, with a sweetness threshold of only 0.28 mg/L (25 nM) is the strongest sweetener known to date. All the new proteins have been produced and purified and the structures of the most powerful mutants have been solved by X-ray crystallography. Docking studies have then confirmed the rationale of their interaction with the human sweet receptor, hinting at a previously unpredicted role of plasticity in said interactio

Mutation of aspartate 238 in FAD synthase isoform 6 increases the specific activity by weakening the FAD binding

FAD synthase (FADS, or FMN:ATP adenylyl transferase) coded by the FLAD1 gene is the last enzyme in the pathway of FAD synthesis. The mitochondrial isoform 1 and the cytosolic isoform 2 are characterized by the following two domains: the C-terminal PAPS domain (FADSy) performing FAD synthesis and pyrophosphorolysis; the N-terminal molybdopterin-binding domain (FADHy) performing a Co++ /K+-dependent FAD hydrolysis. Mutations in FLAD1 gene are responsible for riboflavin responsive and non-responsive multiple acyl-CoA dehydrogenases and combined respiratory chain deficiency. In patients harboring frameshift mutations, a shorter isoform (hFADS6) containing the sole FADSy domain is produced representing an emergency protein. With the aim to ameliorate its function we planned to obtain an engineered more efficient hFADS6. Thus, the D238A mutant, resembling the D181A FMNAT “supermutant” of C. glabrata, was overproduced and purified. Kinetic analysis of this enzyme highlighted a general increase of Km, while the kcat was two-fold higher than that of WT. The data suggest that the FAD synthesis rate can be increased. Additional modifications could be performed to further improve the synthesis of FAD. These results correlate with previous data produced in our laboratory, and point towards the following proposals (i) FAD release is the rate limiting step of the catalytic cycle and (ii) ATP and FMN binding sites are synergistically connected

A Super Stable Mutant of the Plant Protein Monellin Endowed with Enhanced Sweetness

Sweet proteins are a class of proteins with the ability to elicit a sweet sensation in humans upon interaction with sweet taste receptor T1R2/T1R3. Single-chain Monellin, MNEI, is among the sweetest proteins known and it could replace sugar in many food and beverage recipes. Nonetheless, its use is limited by low stability and high aggregation propensity at neutral pH. To solve this inconvenience, we designed a new construct of MNEI, dubbed Mut9, which led to gains in both sweetness and stability. Mut9 showed an extraordinary stability in acidic and neutral environments, where we observed a melting temperature over 20 C higher than that of MNEI. In addition, Mut9 resulted twice as sweet than MNEI. Both proteins were extensively characterized by biophysical and sensory analyses. Notably, Mut9 preserved its structure and function even after 10 min boiling, with the greatest differences being observed at pH 6.8, where it remained folded and sweet, whereas MNEI lost its structure and function. Finally, we performed a 6-month shelf-life assessment, and the data confirmed the greater stability of the new construct in a wide range of conditions. These data prove that Mut9 has an even greater potential for food and beverage applications than MNEI