Food safety

Illness induced by unsafe food is a problem of great public health significance. This study relates exclusively to the occurrence of chemical agents which will result in food unsafe for human consumption since the matter of food safety is of paramount importance in the mission and operation of the manned spacecraft program of the National Aeronautics and Space Administration

Cleavage furrow formation and ingression during animal cytokinesis: a microtubule legacy

Cytokinesis ensures the proper partitioning of the nuclear and cytoplasmic contents into independent daughter cells at the end of cell division. Although the metazoan mitotic spindle has been implicated in the placement and advancement of the cleavage furrow, the molecules responsible for these processes have remained elusive. Recent studies have provided insights into the role of different microtubule structures and associated proteins in cleavage furrow positioning and ingression together with the signalling events that regulate the dynamics of the equatorial cell cortex during cytokinesis. We try to unify these findings into a general model of cytokinesis in which both astral and central spindle microtubules have the ability to induce furrowing. We further propose that the evolutionarily conserved centralspindlin complex serves as a master controller of cell cleavage in Drosophila by promoting both furrow formation and ingression. The same mechanism might be conserved in other organisms

Mutations in sticky lead to defective organization of the contractile ring during cytokinesis and are enhanced by Rho and suppressed by Rac

The contractile ring is a highly dynamic structure, but how this dynamism is accomplished remains unclear. Here, we report the identification and analysis of a novel Drosophila gene, sticky (sti), essential for cytokinesis in all fly proliferating tissues. sti encodes the Drosophila orthologue of the mammalian Citron kinase. RNA interference–mediated silencing of sti in cultured cells causes them to become multinucleate. Components of the contractile ring and central spindle are recruited normally in such STICKY-depleted cells that nevertheless display asymmetric furrowing and aberrant blebbing. Together with an unusual distribution of F-actin and Anillin, these phenotypes are consistent with defective organization of the contractile ring. sti shows opposite genetic interactions with Rho and Rac genes suggesting that these GTPases antagonistically regulate STICKY functions. Similar genetic evidence indicates that RacGAP50C inhibits Rac during cytokinesis. We discuss that antagonism between Rho and Rac pathways may control contractile ring dynamics during cytokinesis

RacGAP50C is sufficient to signal cleavage furrow formation during cytokinesis

Several studies indicate that spindle microtubules determine the position of the cleavage plane at the end of cell division, but their exact role in triggering the formation and ingression of the cleavage furrow is still unclear. Here we show that in Drosophila depletion of either the GAP (GTPase-activating protein) or the kinesin-like subunit of the evolutionary conserved centralspindlin complex prevents furrowing without affecting the association of astral microtubules with the cell cortex. Moreover, time-lapse imaging indicates that astral microtubules serve to deliver the centralspindlin complex to the equatorial cortex just before furrow formation. However, when the GAP-signaling component was mislocalized around the entire cortex using a membrane-tethering motif, this caused ectopic furrowing even in the absence of its motor partner. Thus, the GAP component of centralspindlin is both necessary and sufficient for furrow formation and ingression and astral microtubules provide a route for its delivery to the cleavage site

Development of hemostatic materials made of electrochemically oxidized bacterial cellulose

Book of Abstracts of CEB Annual Meeting 2017[Excerpt] Cellulose is the most abundant polysaccharide in nature, being the main constituent of plant cell walls. It can undergo structural modification by oxidative methods, making it absorbable when implanted in the organism, contrarily to what happens with the non-oxidized cellulose. This way it can be used as raw material in medical devices, such as absorbable hemostatic materials and as a barrier to prevent postoperative adhesions. Cellulose can also be produced by bacteria (mainly from the species Glucanocetobacter xylinus), being this way known as bacterial cellulose (BC). With the increasing use of the hemostatic materials based on oxidized cellulose in surgical procedures, there has also been an increase in the number of case studies that describe post-operative complications associated with the use of these materials. BC has improved characteristics and unique properties compared with polysaccharide derived from plants, namely a higher biocompatibility. It has therefore been the subject of increased research over the past years allowing its application in various fields, especially in biomedical applications [1]. This project aims to develop a hemostatic material to reduce post-operative complications, based on the oxidized BC, using electrochemical oxidation methods [2]. These methods are based on the stable nitroxyl radicals commercially available. [...]info:eu-repo/semantics/publishedVersio

Three-dimensional radiative transfer models of clumpy tori in Seyfert galaxies

Tori of Active Galactic Nuclei are made up of a mixture of hot and cold gas, as well as dust. In order to protect the dust grains from destruction by the hot gas as well as by the energetic radiation of the accretion disk, the dust is often assumed to be distributed in clouds. In our new 3D model of AGN dust tori, the torus is modelled as a wedge-shaped disk in which dusty clouds are randomly distributed, by taking the dust density distribution of the corresponding continuous model into account. We especially concentrate on the differences between clumpy and continuous models in terms of the temperature distributions, the surface brightness distributions and interferometric visibilities, as well as spectral energy distributions. To this end, we employ radiative transfer calculations with the help of the 3D Monte Carlo code MC3D. In a second step, interferometric visibilities are calculated from the simulated surface brightness distributions, which can be directly compared to observations with the MIDI instrument. The radial temperature distributions of clumpy models possess significantly enhanced scatter compared to the continuous cases. Even at large distances, clouds can be heated directly by the central accretion disk. The existence of the silicate 10 micron-feature in absorption or in emission depends sensitively on the distribution, the size and optical depth of clouds in the innermost part of the torus, due to shadowing effects of clouds there. This explains failure and success of previous modelling efforts of clumpy tori. After adapting the parameters of our clumpy standard model to the circumstances of the Seyfert 2 Circinus galaxy, it can qualitatively explain recent mid-infrared interferometric observations performed with MIDI, as well as high resolution spectral data.Comment: 15 pages, 23 figures, accepted by A&

The Infrared Continuum of Active Galaxies

We discuss the different physical processes contributing to the infrared continuum of AGN, assuming that both photoionization from the active center and shocks ionize and heat the gas and dust contained in an ensemble of clouds surrounding the nucleus. Radiation transfer of primary and secondary radiation throughout a cloud is calculated consistently with collisional processes due to the shock. We consider that the observed continuum corresponds to reprocessed radiation from both dust and gas in the clouds. The model is applied to the continuum of Seyfert galaxies from which best estimate of the nuclear, stellar subtracted, emission is available. The results show that radiation-dominated high velocity clouds are more numerous in Seyfert 1-1.5 whereas shock-dominated low velocity clouds are dominant in Seyfert type 2 in full agreement with the unified model for AGN. In type 2 objects, radiation is partly suppressed by a central dusty medium with a high dust-to-gas ratio. A grid of models is used to provide a phenomenological analysis of the observed infrared spectral energy distribution.Comment: 14 pages, 10 figures. in press in MNRA