Annual (2023) taxonomic update of RNA-directed RNA polymerase-encoding negative-sense RNA viruses (realm Riboviria: kingdom Orthornavirae: phylum Negarnaviricota)

55 Pág.In April 2023, following the annual International Committee on Taxonomy of Viruses (ICTV) ratification vote on newly proposed taxa, the phylum Negarnaviricota was amended and emended. The phylum was expanded by one new family, 14 new genera, and 140 new species. Two genera and 538 species were renamed. One species was moved, and four were abolished. This article presents the updated taxonomy of Negarnaviricota as now accepted by the ICTV.This work was supported in part through the Laulima Government Solutions, LLC, prime contract with the U.S. National Institute of Allergy and Infec tious Diseases (NIAID) under Contract No. HHSN272201800013C. J.H.K. performed this work as an employee of Tunnell Government Services (TGS), a subcontractor of Laulima Government Solutions, LLC, under Contract No. HHSN272201800013C. U.J.B. was supported by the Division of Intramural Resarch, NIAID. This work was also funded in part by Contract No. HSHQDC15-C-00064 awarded by DHS S and T for the management and operation of The National Biodefense Analysis and Countermeasures Centre, a federally funded research and development centre operated by the Battelle National Biodefense Institute (V.W.); and NIH contract HHSN272201000040I/HHSN27200004/D04 and grant R24AI120942 (N.V., R.B.T.). S.S. acknowl edges support from the Mississippi Agricultural and Forestry Experiment Station (MAFES), USDA-ARS project 58-6066-9-033 and the National Institute of Food and Agriculture, U.S. Department of Agriculture, Hatch Project, under Accession Number 1021494. The funders had no role in the design of the study; in the collection, analysis, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results. The views and conclusions contained in this document are those of the authors and should not be interpreted as necessarily representing the official policies, either expressed or implied, of the U.S. Department of the Army, the U.S. Department of Defence, the U.S. Department of Health and Human Services, including the Centres for Disease Control and Prevention, the U.S. Department of Homeland Security (DHS) Science and Technology Directorate (S and T), or of the institutions and companies affiliated with the authors. In no event shall any of these entities have any responsibility or liability for any use, misuse, inability to use, or reliance upon the information contained herein. The U.S. departments do not endorse any products or commercial services mentioned in this publication. The U.S. Government retains and the publisher, by accepting the article for publication, acknowledges that the U.S.Government retains a non-exclusive, paid up, irrevocable, world-wide license to publish or reproduce the published form of this manuscript, or allow others to do so, for U.S. Government purposes.Peer reviewe

Building a 3D Printer: Motors and Controls

3D printers require a reliable and robust control system to provide the proper quality of printed parts. The goal of this project was to design and implement the electronic and software controls for the large scale 3D printer. This system must be able to run all three print heads as well as color mixing in the central diamond print head. The resulting design for the control system utilized a RUMBA control board running with Repetier-Firmware as the printer firmware. A separate system was designed for the heat beds, which used an Arduino Uno to control a set of relays to maintain the set temperature to the heat beds

Implementing stakeholder engagement to explore alternative models of consent: An example from the PREP-IT trials

Introduction: Cluster randomized crossover trials are often faced with a dilemma when selecting an optimal model of consent, as the traditional model of obtaining informed consent from participant's before initiating any trial related activities may not be suitable. We describe our experience of engaging patient advisors to identify an optimal model of consent for the PREP-IT trials. This paper also examines surrogate measures of success for the selected model of consent. Methods: The PREP-IT program consists of two multi-center cluster randomized crossover trials that engaged patient advisors to determine an optimal model of consent. Patient advisors and stakeholders met regularly and reached consensus on decisions related to the trial design including the model for consent. Patient advisors provided valuable insight on how key decisions on trial design and conduct would be received by participants and the impact these decisions will have. Results: Patient advisors, together with stakeholders, reviewed the pros and cons and the requirements for the traditional model of consent, deferred consent, and waiver of consent. Collectively, they agreed upon a deferred consent model, in which patients may be approached for consent after their fracture surgery and prior to data collection. The consent rate in PREP-IT is 80.7%, and 0.67% of participants have withdrawn consent for participation. Discussion: Involvement of patient advisors in the development of an optimal model of consent has been successful. Engagement of patient advisors is recommended for other large trials where the traditional model of consent may not be optimal

Annual (2023) taxonomic update of RNA-directed RNA polymerase-encoding negative-sense RNA viruses (realm Riboviria: kingdom Orthornavirae: phylum Negarnaviricota)

Laulima Government Solutions, LLC, prime contract with the U.S. National Institute of Allergy and Infectious Diseases (NIAID) under Contract No. HHSN272201800013C. J.H.K. performed this work as an employee of Tunnell Government Services (TGS), a subcontractor of Laulima Government Solutions, LLC, under Contract No. HHSN272201800013C. U.J.B. was supported by the Division of Intramural Resarch, NIAID. This work was also funded in part by Contract No. HSHQDC15-C-00064 awarded by DHS S and T for the management and operation of The National Biodefense Analysis and Countermeasures Centre, a federally funded research and development centre operated by the Battelle National Biodefense Institute (V.W.); and NIH contract HHSN272201000040I/HHSN27200004/D04 and grant R24AI120942 (N.V., R.B.T.). S.S. acknowledges support from the Mississippi Agricultural and Forestry Experiment Station (MAFES), USDA-ARS project 58-6066-9-033 and the National Institute of Food and Agriculture, U.S. Department of Agriculture, Hatch Project, under Accession Number 1 021 494.National Institutes of Health. National Institute of Allergy and Infectious Disease. Division of Clinical Research. Integrated Research Facility at Fort Detrick. Frederick, MD, USA.Hokkaido Research Organization. Agricultural Research Department. Ornamental Plants and Vegetables Research Center. Takikawa, Hokkaido, Japan.United States Department of Agriculture. Agricultural Research Service. US Horticultural Research Laboratory. Fort Pierce, FL, USA.D.I. Ivanovsky Institute of Virology of N.F. Gamaleya National Center on Epidemiology and Microbiology of Ministry of Health of Russian Federation. Moscow, Russia.University of Ljubljana. Faculty of Medicine. Institute of Microbiology and Immunology. Ljubljana, Slovenia.Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria - Campus de Montegancedo. Departamento de Biotecnología-Biología Vegetal. Centro de Biotecnología y Genómica de Plantas. Pozuelo de Alarcón / Universidad Politécnica de Madrid. Escuela Técnica Superior de Ingeniería Agronómica, Alimentaria y de Biosistemas. Madrid, Spain.University of Georgia. Insitute of Bioinformatics. Department of Infectious Diseases, Department of Epidemiology and Biostatistics. Center for Ecology of Infectious Diseases. Athens, GA, USA.Greifswald-Insel Riems. Institute of Novel and Emerging Infectious Diseases. Friedrich-Loeffler-Institut. Greifswald, Germany.Mississippi State University. Department of Biological Sciences. Mississippi State, MS, USA.ICAR-Indian Agricultural Research Institute. Division of Plant Pathology. New Delhi, India.Friedrich-Loeffler-Institut. Institute of Diagnostic Virology. Greifswald-Insel Riems, Germany.Instituto Nacional de Tecnología Agropecuaria-Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatologia y Modelizacion Agricola. Córdoba, Argentina.Centers for Disease Control and Prevention. Viral Special Pathogens Branch. Division of High-Consequence Pathogens and Pathology. Atlanta, GA, USA.Philipps-University Marburg. Institute of Virology. Marburg, Germany.Colorado State University. Department of Microbiology, Immunology and Pathology. Fort Collins, CO, USA.Australian Centre for Disease Preparedness, Geelong. Commonwealth Scientific and Industrial Research Organisation. Australia.Agroscope. Virology-Phytoplasmology Laboratory. Nyon, Switzerland.University of New Mexico Health Sciences Center. Albuquerque, NM, USA.Columbia University. Mailman School of Public Health. Center for Infection and Immunity, and Department of Epidemiology. New York, USA.French Agency for Food, Environmental and Occupational Heath Safety. Laboratory of Ploufragan-Plouzané-Niort. Ploufragan, France.National Institutes of Health. National Institute of Allergy and Infectious Diseases. Laboratory of Infectious Diseases. RNA Viruses Section. Bethesda, MD, USA.University of California. Department of Molecular Biology and Biochemistry. Irvine, CA, USA.The University of Texas Medical Branch at Galveston. Galveston, TX, USA.University of the Free State. National Health Laboratory Service and Division of Virology. Division of Virology. Bloemfontein, South Africa.Faculty of Life Sciences, Humboldt-Universität zu Berlin. Division Phytomedicine. Berlin, Germany.Colorado State University. Fort Collins, CO, USA.Southwest University. Citrus Research Institute. National Citrus Engineering and Technology Research Center. Beibei, Chongqing, PR China.Instituto de Salud Carlos III. National Microbiology Center. Respiratory Virus and Influenza Unit. Madrid, Spain.Albert Einstein College of Medicine. Department of Microbiology and Immunology. Bronx, NY, USA.Unite des Virus Emergents -Aix-Marseille Univ-IRD 190-Inserm 1207. Marseille, France.ICAR-Indian Agricultural Statistics Research Institute. Centre for Agricultural Bioinformatics. New Delhi, India.The New Zealand Institute for Plant and Food Research Limited. Auckland, New Zealand.National Institutes of Health. National Institute of Allergy and Infectious Diseases. Integrated Research Facility at Fort Detrick. Frederick, MD, USA.Universidad de La Plata. Facultad de Ciencias Agrarias y Forestales. CIDEFI. La Plata, Argentina.The Scripps Research Institute. Department of Immunology and Microbiology IMM-6. La Jolla, CA, USA.The University of Texas Medical Branch at Galveston. Department of Microbiology and Immunology. World Reference Center for Emerging Viruses and Arboviruses. Galveston, TX, USA.Wageningen Bioveterinary Research. Department of Virology. Lelystad, Netherlands.Instituto de Patología Vegetal. 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School of Life Sciences. Coventry, UK.Hacettepe University. Faculty of Medicine. Department of Medical Microbiology. Virology Unit. Ankara, Turkey.Museum Support Center. Smithsonian Institution. Walter Reed Biosystematics Unit. Suitland, MD, USA / Walter Reed Army Institute of Research. One Health Branch. Silver Spring, MD, USA / Smithsonian Institution-National Museum of Natural History. Department of Entomology. Washington, DC, USA / China National Rice Research Institute. Hangzhou, PR China.University of Cambridge. Department of Pathology. Cambridge, UK.Animal and Plant Health Agency. eybridge, Surrey, UK.World Health Organization. Geneva, Switzerland.Embrapa Cassava and Fruits. Cruz das Almas, BA, Brazil.Martin Luther University Halle-Wittenberg. Institute of Biochemistry and Biotechnology. Halle/Saale, Germany.Instituto de Biotecnología y Biología Molecular. Facultad de Ciencias Exactas. La Plata, Argentina.Icahn School of Medicine at Mount Sinai. New York, NY, USA.Brandenburg State Office of Rural Development. Agriculture and Land Consolidation. Frankfurt, Germany.Humboldt-Universität Zu Berlin. Thaer-Institute of Agricultural and Horticultural Sciences. Division Phytomedicine. Berlin, Germany.Ministry of Education. Jilin University. College of Veterinary Medicine. Key Laboratory of Zoonoses Research. State Key Laboratory for Zoonotic Diseases. Changchun, PR China / Georgetown University. School of Medicine. Division of Biomedical Graduate Research Organization. Department of Microbiology and Immunology. Washington, DC, USA.Institute of Vertebrate Biology of the Czech Academy of Sciences. Brno, CzechiaBoston University. National Emerging Infectious Diseases Laboratories. Chobanian and Avedisian School of Medicine. Department of Virology, Immunology and Microbiology. Boston, MA, USA.Friedrich-Loeffler-Institut. Institute of Novel and Emerging Infectious Diseases. Greifswald-Insel Riems, Germany.Bernhard-Nocht Institute for Tropical Medicine. WHO Collaborating Centre for Arboviruses and Hemorrhagic Fever Reference and Research. Department of Virology. Hamburg, Germany.United States Department of Agriculture. Agricultural Research Service / United States Naval Academy. Floral and Nursery Plants Research Unit. Beltsville, MD, USA.Hosei University. Department of Clinical Plant Science. Koganei, Tokyo, Japan.Kochi Agricultural Research Center. Nankoku, Kochi, Japan.University of Helsinki. Department of Virology. Medicum, Helsinki, Finland.University of Zurich. Institute of Veterinary Pathology, Vetsuisse Faculty. Zurich, Switzerland.Auckland University of Technology. The School of Science. Auckland, New Zealand.Yamagata University. Faculty of Medicine. Department of Infectious Diseases. Yamagata, Japan / Osaka Metropolitan University. Graduate School of Veterinary Science / Osaka Metropolitan University. International Research Center for Infectious Diseases. Izumisano, Osaka, Japan.Centers for Disease Control and Prevention. Fort Collins, CO, USA.Murdoch University. School of Veterinary Medicine. Murdoch, WA, Australia.Kobe University. Graduate School of Agricultural Science. Kobe, Hyogo, Japan.Huazhong Agricultural University. State Key Laboratory of Agricultural Microbiology. Wuhan, Hubei Province, PR China.International Rice Research Institute. College. Los Baños, Laguna, Philippines.Charité-Universitätsmedizin Berlin. Freie Universität Berlin. Corporate Member. Humboldt-Universität zu Berlin. Berlin Institute of Health. Institute of Virology. Berlin, Germany.Slovak Academy of Sciences. Biomedical Research Center. Institute of Virology. Bratislava, Slovakia.Linköping University. Department of Biomedical and Clinical Sciences. Linköping, Sweden.Okayama University. Institute of Plant Science and Resources. Kurashiki, Japan.National Institutes of Health. National Library of Medicine. 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School of Biological Sciences. Auckland, New Zealand.KU Leuven. Rega Institute. Zoonotic Infectious Diseases unit. Leuven, Belgium.FIND - The Global Alliance for Diagnostics. Geneva, Switzerland.Pontificia Universidad Católica de Valparaíso-Campus Curauma. Instituto de Biología-Laboratorio de Genética Molecular. Valparaíso, Chile.United States Department of Agriculture. Agricultural Research Service. Toledo, OH, USA.Folkhalsomyndigheten. Stockholm, Sweden.Yamagata University. Department of Agriculture. Tsuruoka, Japan.Washington State University. Irrigated Agricultural Research and Extension Center. Department of Plant Pathology. Prosser, WA, USA.Utsunomiya University. Utsunomiya, Japan.Universitat Politècnica de València-Consejo Superior de Investigaciones Científicas. Instituto de Biología Molecular y Celular de Plantas. Valencia, Spain.Utsunomiya University. School of Agriculture. Utsunomiya, Japan.Novosibirsk State University. Novosibirsk Oblast, Russia.University of Wisconsin-Madison. Department of Pathobiological Sciences. Influenza Research Institute. Madison, USA.University of Veterinary Medicine Vienna. Institute of Virology. Vienna, Austria.Mohammed Bin Rashid University of Medicine and Health Sciences. College of Medicine. Dubai, United Arab Emirates.Ministério da Saúde. Secretaria de Vigilância em Saúde e Ambiente. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Oklahoma State University. Institute for Biosecurity and Microbial Forensics. Stillwater. Oklahoma, USA.Icahn School of Medicine at Mount Sinai. Department of Microbiology. New York, NY, USA.Universitat Politècnica de Valencia - Consejo Superior de Investigaciones Cientificas. Instituto de Biología Molecular y Celular de Plantas. Valencia, Spain.Aristotle University of Thessaloniki. Department of Microbiology, Medical School. National Reference Centre for Arboviruses and Haemorrhagic Fever viruses. Thessaloniki, Greece.Robert Koch Institute. Genome Competence Center. Berlin, Germany / Cornell University. College of Veterinary Medicine. Baker Institute for Animal Health. Ithaca, NY, USA.Ministério da Saúde. Secretaria de Vigilância em Saúde e Ambiente. Instituto Evandro Chagas. Ananindeua, PA, Brasil.National Institute for Communicable Diseases of the National Health Laboratory Service. Center for Emerging Zoonotic and Parasitic Diseases. Sandringham-Johannesburg, Gauteng, South Africa.University of Georgia. College of Veterinary Medicine. Department of Population Health. Athens, GA, USA.Georgia State University Institute for Biomedical Sciences. Center for Translational Antiviral Research. Atlanta, GA, USA.International AIDS Vaccine Initiative. Vaccine Design and Development Laboratory. Brooklyn, NY, USA.Auckland University of Technology. The School of Science / The New Zealand Institute for Plant and Food Research Limited. 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Raleigh, NC, USA.Chinese Academy of Sciences. Wuhan Institute of Virology. Key Laboratory of Special Pathogens and Biosafety. Wuhan, PR China.Agricultural University of Athens. School of Agricultural Production, Infrastructure and Environment. Department of Crop Science. Plant Pathology Laboratory. Votanikos, Athens, Greece.Yokohama Plant Protection Station. Yokohama, Kanagawa, Japan.Zhejiang University. Institute of Insect Sciences. Hangzhou, PR China.Universidade Federal de Viçosa. Dep. de Fitopatologia/BIOAGRO. Viçosa, MG, Brazil.Center for Disease Control and Prevention of Xinjiang Military Command Area. Xinjiang, PR China.Guangxi Academy of Specialty Crops. Guangxi, PR China / Fudan University. School of Life Sciences and Human Phenome Institute. Shanghai, PR China.University of Chinese Academy of Sciences. Beijing, PR China.Pharmaq Analytiq. Bergen, Norway.Francis Crick Institute. Worldwide Influenza Centre. London, UK.Navarro, BeatrizIn April 2023, following the annual International Committee on Taxonomy of Viruses (ICTV) ratification vote on newly proposed taxa, the phylum Negarnaviricota was amended and emended. The phylum was expanded by one new family, 14 new genera, and 140 new species. Two genera and 538 species were renamed. One species was moved, and four were abolished. This article presents the updated taxonomy of Negarnaviricota as now accepted by the ICTV