Der Film als Medium von Vergangenheit und Gegenwart

Im theoretischen Teil dieser Arbeit geht es darum, wie Geschichte, hier im Besonderen die des Zweiten Weltkrieges, im Film dargestellt werden kann und darf und darum, ob das Medium für eine ernst zu nehmende Auseinandersetzung mit der Vergangenheit überhaupt geeignet Ist. Besonders der Spielfilm bietet hier Vorteile, die dem Text und damit den Geschichtsbüchern unzugänglich sind, was es notwendig macht, ihn auch im wissenschaftlichen Diskurs ernst zu nehmen. Die Darstellung des Zweiten Weltkrieges im Spielfilm ist nicht gleichbleibend, sonder verändert sich. Da die dargestellte Geschichte vergangen und unabänderlich ist, können diese Entwicklungen in der Darstellungsweise nur von der Filmproduktionsgesellschaft herrühren. Im analytischen Teil meiner Arbeit stelle ich daher fest, dass Filme, welche den Zweiten Weltkrieg darstellen immer auch einen Mehrgehalt aufweisen. Durch ihre Art der Darstellung trifft die Produktionsgesellschaft des Filmes eine Aussage nicht nur zur dargestellten Geschichte, sondern auch über sich selbst. Durch die Untersuchung verschiedener Gesellschaftskontexte sowie daraus entstandener Filme belege ich in dieser Arbeit, dass im populären Spielfilm niemals nur eine Geschichte, in diesem Fall die des Zweiten Weltkrieges, erzählt wird, sondern immer auch die Geschichte der Produktionsgesellschaft in den Film miteinfließt. Als geografischen und zeitlichen Kontext habe ich mir hierfür deutsche sowie amerikanische Produktionen von den 1950ern bis 2009 ausgewählt. Wird die Geschichte hier in beiden Nationen gleich erzählt oder gibt es Unterschiede und wie wirkt sich der zunehmende Abstand zu den geschichtlichen Ereignissen in den unterschiedlichen Kontexten aus? Um Veränderungen und Unterschiede genau feststellen zu können war es nötig die Analyse nicht auf die Filme im Allgemeinen sondern auf ein gleichbleibendes Element innerhalb dieser Filme zu konzentrieren. Meine Wahl fiel hierbei auf die Figur des deutschen Nazis, welche in vielen Spielfilmen über den Zweiten Weltkrieg eine zentrale Rolle spielt. Die genaue Analyse eines in allen Beispielen vorhandenen Filmmotivs, sowie die Auseinandersetzung mit den unterschiedlichen Produktionskontexten erlauben mir festzustellen, inwieweit sich aktuelle Themen und Ansichten der Produktionsgesellschaft in diesem Motiv wiederfinden lassen und in welchem Maße sich die Darstellung des Motivs von Kontext zu Kontext unterscheidet und im Laufe der Zeit verändert

A Machine Learning-Based Raman Spectroscopic Assay for the Identification of Burkholderia mallei and Related Species

Burkholderia (B.) mallei, the causative agent of glanders, and B. pseudomallei, the causative agent of melioidosis in humans and animals, are genetically closely related. The high infectious potential of both organisms, their serological cross-reactivity, and similar clinical symptoms in human and animals make the differentiation from each other and other Burkholderia species challenging. The increased resistance against many antibiotics implies the need for fast and robust identification methods. The use of Raman microspectroscopy in microbial diagnostic has the potential for rapid and reliable identification. Single bacterial cells are directly probed and a broad range of phenotypic information is recorded, which is subsequently analyzed by machine learning methods. Burkholderia were handled under biosafety level 1 (BSL 1) conditions after heat inactivation. The clusters of the spectral phenotypes and the diagnostic relevance of the Burkholderia spp. were considered for an advanced hierarchical machine learning approach. The strain panel for training involved 12 B. mallei, 13 B. pseudomallei and 11 other Burkholderia spp. type strains. The combination of top- and sub-level classifier identified the mallei-complex with high sensitivities (>95%). The reliable identification of unknown B. mallei and B. pseudomallei strains highlighted the robustness of the machine learning-based Raman spectroscopic assay

Simulation of astronomical aspects of Middle Neolithic circular ditch systems

Introduction The oldest Middle European monuments (approx. 4850/4750 – 4550/4500 BC) were presented in the Lower Austrian County Exhibition at Heldenberg in 2005 (Daim & Neubauer, 2005). The Middle Neolithic circular ditch systems, or Kreisgrabenanlagen (KGA), are known all over Middle Europe. These wooden monuments form a first pan-European phenomenon, crossing the cultural borders defined by the archaeological material record. One third of the approx. 135 monuments known so far are found in..

How much rain is too much for a GPR survey? Results of the Borre Monitoring Project

Soil moisture variation is complex and depends on a range of factors, which complicates the formulation of recommendations for GPR surveys. Low amounts of soil moisture produced GPR data of higher quality. However, precipitation rates as well as chronological sequence of precipitation/thawing processes and the GPR survey are of importance. Winter months can offer favorable conditions for GPR surveys if temperatures remain negative over a prolonged time period, allowing for frost to build in the ground. Results of the Borre Monitoring Project (BMP) are valid only for sites with similar settings as Borre; the monitoring approach, however, can be transferred to larger regions with more representative sites

Der Studien- und Berufserfolg von (angehenden) Lehrkräften in Österreich im Längsschnitt – Ausgewählte Befunde aus dem ersten Studienjahr

Es wird eine Längsschnittstudie vorgestellt, in der zwei Kohorten von Studierenden vom Aufnahmeverfahren beginnend über den Verlauf des Studiums bis in den Beruf begleitet werden. Über jeweils fünf Jahre hinweg sollen Studienerfolg und Praxiserfahrungen erhoben und in Beziehung zu den Ergebnissen des Aufnahmeverfahrens gesetzt werden. In diesem Beitrag werden neben der Konzeption der Studie Ergebnisse einer Nacherhebung im ersten Semester präsentiert (Nt2 = 781, Nt2a = 241), die darauf hinweisen, dass von den erhobenen Merkmalen vor allem die Sprachkompetenz und bestimmte Persönlichkeitsmerkmale prädiktiv für die Studienleistungen und die Zufriedenheit mit der Studien- und Berufswahl sind

Swiss general internal medicine board examination: quantitative effects of publicly available and unavailable questions on question difficulty and test performance.

BACKGROUND Formerly, a substantial number of the 120 multiple-choice questions of the Swiss Society of General Internal Medicine (SSGIM) board examination were derived from publicly available MKSAP questions (Medical Knowledge Self-Assessment Program®). The possibility to memorise publicly available questions may unduly influence the candidates' examination performance. Therefore, the examination board raised concerns that the examination did not meet the objective of evaluating the application of knowledge. The society decided to develop new, "Helvetic" questions to improve the examination. The aim of the present study was to quantitatively assess the degree of difficulty of the Helvetic questions (HQ) compared with publicly available and unavailable MKSAP questions and to investigate whether the degree of difficulty of MKSAP questions changed over time as their status changed from publicly available to unavailable. METHODS The November 2019 examination consisted of 40 Helvetic questions, 40 publicly available questions from MKSAP edition 17 (MKSAP-17) and 40 questions from MKSAP-15/16, which were no longer publicly available at the time of the examination. An one factorial univariate analysis of variance (ANOVA) examined question difficulty (lower values mean higher difficulty) between these three question sets. A repeated ANOVA compared the difficulty of MKSAP-15/16 questions in the November 2019 examination with the difficulty of the exact same questions from former examinations, when these questions belonged to the publicly available MKSAP edition. The publicly available MKSAP-17 and the publicly unavailable Helvetic questions served as control. RESULTS The analysis of the November 2019 exam showed a significant difference in average item difficulty between Helvetic and MKSAP-17 questions (71% vs 86%, p <0.001) and between MKSAP-15/16 and MKSAP-17 questions (70% vs 86%, p <0.001). There was no significant difference in item difficulty between Helvetic and MKSAP-15/16 questions (71% vs 70%, p = 0.993). The repeated measures ANOVA on question use and the three question categories showed a significant interaction (p <0.001, partial eta-squared = 0.422). The change in the availability of MKSAP-15/16 questions had a strong effect on difficulty. Questions became on average 21.9% more difficult when they were no longer publicly available. In contrast, the difficulty of the MKSAP-17 and Helvetic questions did not change significantly across administrations. DISCUSSION This study provides the quantitative evidence that the public availability of questions has a decisive influence on question difficulty and thus on SSGIM board examination performance. Reducing the number of publicly available questions in the examination by introducing confidential, high-quality Helvetic questions contributes to the validity of the board examination by addressing higher order cognitive skills and making rote-learning strategies less effective

Transcriptional Profiling of the Dose Response: A More Powerful Approach for Characterizing Drug Activities

The dose response curve is the gold standard for measuring the effect of a drug treatment, but is rarely used in genomic scale transcriptional profiling due to perceived obstacles of cost and analysis. One barrier to examining transcriptional dose responses is that existing methods for microarray data analysis can identify patterns, but provide no quantitative pharmacological information. We developed analytical methods that identify transcripts responsive to dose, calculate classical pharmacological parameters such as the EC50, and enable an in-depth analysis of coordinated dose-dependent treatment effects. The approach was applied to a transcriptional profiling study that evaluated four kinase inhibitors (imatinib, nilotinib, dasatinib and PD0325901) across a six-logarithm dose range, using 12 arrays per compound. The transcript responses proved a powerful means to characterize and compare the compounds: the distribution of EC50 values for the transcriptome was linked to specific targets, dose-dependent effects on cellular processes were identified using automated pathway analysis, and a connection was seen between EC50s in standard cellular assays and transcriptional EC50s. Our approach greatly enriches the information that can be obtained from standard transcriptional profiling technology. Moreover, these methods are automated, robust to non-optimized assays, and could be applied to other sources of quantitative data

Expression of the embryonic stem cell marker SOX2 in early-stage breast carcinoma

<p>Abstract</p> <p>Background</p> <p>The SRY-related HMG-box family of transcription factors member <it>SOX2 </it>has been mainly studied in embryonic stem cells as well as early foregut and neural development. More recently, SOX2 was shown to participate in reprogramming of adult somatic cells to a pluripotent stem cell state and implicated in tumorigenesis in various organs. In breast cancer, SOX2 expression was reported as a feature of basal-like tumors. In this study, we assessed SOX2 expression in 95 primary tumors of postmenopausal breast cancer patients.</p> <p>Methods</p> <p>Samples from 95 patients diagnosed and treated at the University of Tuebingen Institute of Pathology and Women's Hospital were analyzed by immunohistochemistry for SOX2 expression in the primary tumor samples and in corresponding lymph node metastasis, where present. Furthermore, SOX2 amplification status was assessed by FISH in representative samples. In addition, eighteen fresh frozen samples were analyzed for <it>SOX2</it>, <it>NANOG </it>and <it>OCT4 </it>gene expression by real-time PCR.</p> <p>Results</p> <p>SOX2 expression was detected in 28% of invasive breast carcinoma as well as in 44% of ductal carcinoma in situ (DCIS) lesions. A score of SOX2 expression (score 0 to 3) was defined in order to distinguish SOX2 negative (score 0) from SOX2 positive samples (score 1-3) and among latter the subgroup of SOX2 high expressors (score 3 > 50% positive cells). Overall, the incidence of SOX2 expression (score 1-3) was higher than previously reported in a cohort of lymph node negative patients (28% versus 16.7%). SOX2 expression was detected across different breast cancer subtypes and did not correlate with tumor grading. However, high SOX2 expression (score 3) was associated with larger tumor size (p = 0.047) and positive lymph node status (0.018). Corresponding metastatic lymph nodes showed higher SOX2 expression and were significantly more often SOX2 positive than primary tumors (p = 0.0432).</p> <p>Conclusions</p> <p>In this report, we show that the embryonic stem cell factor SOX2 is expressed in a variety of early stage postmenopausal breast carcinomas and metastatic lymph nodes. Our data suggest that SOX2 plays an early role in breast carcinogenesis and high expression may promote metastatic potential. Further studies are needed to explore whether SOX2 can predict metastatic potential at an early tumor stage.</p