361 research outputs found
Clogging by sieving in microchannels: Application to the detection of contaminants in colloidal suspensions
We report on a microfluidic method that allows measurement of a small
concentration of large contaminants in suspensions of solid micrometer-scale
particles. To perform the measurement, we flow the colloidal suspension through
a series of constrictions, i.e. a microchannel of varying cross-section. We
show and quantify the role of large contaminants in the formation of clogs at a
constriction and the growth of the resulting filter cake. By measuring the time
interval between two clogging events in an array of parallel microchannels, we
are able to estimate the concentration of contaminants whose size is selected
by the geometry of the microfluidic device. This technique for characterizing
colloidal suspensions offers a versatile and rapid tool to explore the role of
contaminants on the properties of the suspensions
Influence of the initial chemical conditions on the rational design of silica particles
The influence of the water content in the initial composition on the size of silica particles produced using the Stöber process is well known. We have shown that there are three morphological regimes defined by compositional boundaries. At low water levels (below stoichiometric ratio of water:tetraethoxysilane), very high surface area and aggregated structures are formed; at high water content (>40 wt%) similar structures are also seen. Between these two boundary conditions, discrete particles are formed whose size are dictated by the water content. Within the compositional regime that enables the classical Stöber silica, the structural evolution shows a more rapid attainment of final particle size than the rate of formation of silica supporting the monomer addition hypothesis. The clearer understanding of the role of the initial composition on the output of this synthesis method will be of considerable use for the establishment of reliable reproducible silica production for future industrial adoption
Development of ISB 1442, a CD38 and CD47 bispecific biparatopic antibody innate cell modulator for the treatment of multiple myeloma
Antibody engineering can tailor the design and activities of therapeutic antibodies for better efficiency or other advantageous clinical properties. Here we report the development of ISB 1442, a fully human bispecific antibody designed to re-establish synthetic immunity in CD38+ hematological malignancies. ISB 1442 consists of two anti-CD38 arms targeting two distinct epitopes that preferentially drive binding to tumor cells and enable avidity-induced blocking of proximal CD47 receptors on the same cell while preventing on-target off-tumor binding on healthy cells. The Fc portion of ISB 1442 is engineered to enhance complement dependent cytotoxicity, antibody dependent cell cytotoxicity and antibody dependent cell phagocytosis. ISB 1442 thus represents a CD47-BsAb combining biparatopic targeting of a tumor associated antigen with engineered enhancement of antibody effector function to overcome potential resistance mechanisms that hamper treatment of myeloma with monospecific anti-CD38 antibodies. ISB 1442 is currently in a Phase I clinical trial in relapsed refractory multiple myeloma
"Nanohybrids" based on pH-responsive hydrogels and inorganic nanoparticles for drug delivery and sensor applications.
Allyl-PEG capped inorganic NPs, including magnetic iron oxide (IONPs), fluorescent CdSe/ZnS quantum dots (QDs), and metallic gold (AuNPs of 5 and 10 nm) both individually and in combination, were covalently attached to pH-responsive poly(2-vinylpyridine-co-divinylbenzene) nanogels via a facile and robust one-step surfactant-free emulsion polymerization procedure. Control of the NPs associated to the nanogels was achieved by the late injection of the NPs to the polymerization solution at a stage when just polymeric radicals were present. Remarkably, by varying the total amount of NPs injected, the swelling behavior could be affected. Furthermore, the magnetic response as well as the optical features of the nanogels containing either IONPs or QDs could be modified. In addition, a radical quenching in case of gold nanoparticles was observed, thus affecting the final nanogel geometry
Clinical profiling of specific diagnostic subgroups of women with chronic pelvic pain
Introduction: Chronic pelvic pain (CPP) is a common condition affecting up to 26.6% of women, with many suffering for several years before diagnosis and/or treatment. Its clinical presentation is varied and there are frequently comorbid conditions both within and outside the pelvis. We aim to explore whether specific subgroups of women with CPP report different clinical symptoms and differing impact of pain on their quality of life (QoL).
Methods: The study is part of the Translational Research in Pelvic Pain (TRiPP) project which is a cross-sectional observational cohort study. The study includes 769 female participants of reproductive age who completed an extensive set of questions derived from standardised WERF EPHect questionnaires. Within this population we defined a control group (reporting no pelvic pain, no bladder pain syndrome, and no endometriosis diagnosis, Nâ=â230) and four pain groups: endometriosis-associated pain (EAP, Nâ=â237), interstitial cystitis/bladder pain syndrome (BPS, Nâ=â72), comorbid endometriosis-associated pain and BPS (EABP, Nâ=â120), and pelvic pain only (PP, Nâ=â127).
Results: Clinical profiles of women with CPP (13â50 years old) show variability of clinical symptoms. The EAP and EABP groups scored higher than the PP group (pâpâpâpâpâpâpâ
Discussion: Our results demonstrate the negative impact that chronic pain has on CPP patients' QoL and reveal an increased negative impact of pain on the comorbid EABP group. Furthermore, it demonstrates the importance of dyspareunia in women with CPP. Overall, our results demonstrate the need for further exploration of interventions targeting QoL more broadly and suggest that novel approaches to classifying women with CPP are needed
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A mucosal vaccine against Chlamydia trachomatis generates two waves of protective memory T cells
Genital Chlamydia trachomatis (Ct) infection induces protective immunity that depends on interferon-Îł producing CD4 T-cells. By contrast, mucosal exposure to ultraviolet light (UV)-inactivated Ct (UV-Ct) generated regulatory T-cells that exacerbated subsequent Ct infection. We show that mucosal immunization with UV-Ct complexed with charge-switching synthetic adjuvant particles (cSAP) elicited long-lived protection in conventional and humanized mice. UV-Ct-cSAP targeted immunogenic uterine CD11b+CD103â dendritic cells (DCs), whereas UV-Ct accumulated in tolerogenic CD11bâCD103+ DCs. Regardless of vaccination route, UV-Ct-cSAP induced systemic memory T-cells, but only mucosal vaccination induced effector T-cells that rapidly seeded uterine mucosa with resident memory T-cells (TRM). Optimal Ct clearance required both TRM seeding and subsequent infection-induced recruitment of circulating memory T-cells. Thus, UV-Ct-cSAP vaccination generated two synergistic memory T-cell subsets with distinct migratory properties
Exploring the anomaly in the interaction cross section and matter radius of 23O
New measurements of the interaction cross sections of 22,23O at 900A MeV
performed at the GSI, Darmstadt are reported that address the unsolved puzzle
of the large cross section previously observed for 23O. The matter radii for
these oxygen isotopes extracted through a Glauber model analysis are in good
agreement with the new predictions of the ab initio coupled-cluster theory
reported here. They are consistent with a 22O+neutron description of 23O as
well.Comment: 4 pages, 3 figure
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