Recombinant 60-kda Heat Shock Protein From Paracoccidioides Brasiliensis: Is It A Good Antigen For Serological Diagnosis Of Paracoccidioidomycosis?

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Paracoccidioides brasiliensis and P. lutzii are fungi that cause paracoccidioidomycosis (PCM), the most prevalent systemic mycosis in South America. For serological diagnosis, although 43-kDa glycoprotein (gp43) is regarded as highly specific for PCM, the occurrence of false negative reactions in sera from patients infected with P. lutzii suggests that preparation with only one antigen is not recommended. Heat shock proteins are feasible alternatives as a second antigen because they are often highly immunogenic. In this study, we evaluated the usefulness of recombinant 60-kDa heat shock protein from P. brasiliensis (rPbHsp60) for the serological diagnosis of PCM. Using western blotting assay, we observed that 77.3% of the sera from PCM patients were positive to rPbHsp60, with 90.9% positivity to recombinant gp43 (rgp43). More importantly, sera from healthy subjects had 27% positivity to rPbHsp60 and none to rgp43. When rPbHsp60 was used in ELISA, we did not observe significant differences between the reactions with sera from PCM patients and healthy subjects, while the difference was clearly evident when the antigen was rgp43. Furthermore, rPbHsp60 was recognized by sera from patients with histoplasmosis, aspergillosis, sporotrichosis or tuberculosis in an ELISA test. These results show that rPbHsp60 is not a good antigen for PCM diagnosis.504Sao Paulo Research Foundation (FAPESP) [2013/12278-3]FAPESP [2012/07924-0, 2009/03235-3, 2012/08552-0]Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP

proposal of a methodology for achieving a leed o m certification in historic buildings

Abstract Nowadays resources are running out quickly, it's necessary to consider how the construction industry influences the environment using different materials and sources during all the building's life cycle. For this reason, in every transformation phases it's necessary to consider concepts as sustainability and green buildings. These are diffused from hundreds kind of green assessment tools, developed to measure sustainability goals in building sector and to compare the project with possible best practices or other green buildings. In this background, the rating system LEED (Leadership in Energy and Environmental Design) aims to examine and classify buildings according to energetic and environmental requirements. The particular LEED O+M (Building Operations and Maintenance) is developed for existing buildings undergoing improvement work or little to no construction and is based on the operative and management aspects. The certification process results, at a first analysis, hard to follow due to the complexity of internal parameters and the documentation required. The paper consists in a methodology and in an univocal work program of LEED O+M, trying to obtain the minimum requested certification score with optimization of the technical resources and documents. This methodology has application in a case study of historic building: the Ca' Rezzonico Museum, in the center of Venice

Cost-Optimal measures for renovation of existing school buildings towards nZEB

Abstract The energy policies of the European Union (EU) encourage the member states to convert building stock into nearly Zero-Energy Buildings (nZEB) and national public authorities to adopt exemplary actions. Directive 2010/31/EU (EPBD recast) introduces the concept of nZEB as a building that has a very high energy performance and its energy need is covered to a very significant extent by energy from renewable sources (RES). Moreover the Directive refers to the cost-optimal methodology for fixing building energy requirements. This paper presents the results of the application of the cost-optimal methodology in a couple of existing school buildings located in the North East of Italy. The analysed buildings are a primary and a secondary schools that differ in construction period, in compactness ratio, in buildings envelope materials and systems. Several combinations of retrofit measures have been applied in order to derive cost-effective efficient solutions for retrofitting according to the methodology proposed by the project Annex56 "Cost Effective Energy & CO2 Emissions Optimization in Building Renovation". The cost-optimal level has been identified for each building and the best performing solutions have been selected considering a financial analysis and the application of "Conto Termico 2.0" government incentives. The results show the suitability of the proposed methodology to assess cost-optimality and energy efficiency in school building refurbishment. Moreover, this study shows different possibility providing the most cost-effective balance between costs and energy saving

Retrofit of an Historical Building toward NZEB

Abstract The European Directive on Energy Efficiency in Buildings (Directive 2010/31/EU) has introduced the need to transform buildings to nearly zero energy (NZEB) by 2020. Existing buildings represent the major part of the building stock and an interesting challenge is to transfer it toward NZEB. Energy retrofit is even more significant in Italy, where existing buildings stock (mainly residential) is also historic, so it's subject to environmental constraints or architectural-artistic value, and it's influenced by specific regulations and methods of intervention for refurbishment. In this case, the challenge becomes even more important and concerns both the building shell and the systems: retrofitting introduces not originally present in the complex; retrofit is not covered in the maintenance, since it represents an upgrade, an adaptation of the building, specifically in relation to energy efficiency, but also, by extension, other functions / features pertaining to the environment and sustainability. A case study of a radical refurbishment of an historical building is Ca' S. Orsola in Treviso. It is ruled by the Historical and Architectural Veneto Regional Authority. The building has been transformed into a prestigious residential complex by a major renovation that was aimed primarily seismic and energy upgrading. The energy and environmental performance of building have been analyzed by numerical simulation and experimental measurement in the EBC IEA Annex 56 [1] context with the aim to verify that intervention strategies respect to the reduction of energy consumption, the minimization of CO 2 emissions and maximizing the use of sources of renewable energy

Statistical pairwise interaction model of stock market

Financial markets are a classical example of complex systems as they comprise many interacting stocks. As such, we can obtain a surprisingly good description of their structure by making the rough simplification of binary daily returns. Spin glass models have been applied and gave some valuable results but at the price of restrictive assumptions on the market dynamics or others are agent-based models with rules designed in order to recover some empirical behaviours. Here we show that the pairwise model is actually a statistically consistent model with observed first and second moments of the stocks orientation without making such restrictive assumptions. This is done with an approach based only on empirical data of price returns. Our data analysis of six major indices suggests that the actual interaction structure may be thought as an Ising model on a complex network with interaction strengths scaling as the inverse of the system size. This has potentially important implications since many properties of such a model are already known and some techniques of the spin glass theory can be straightforwardly applied. Typical behaviours, as multiple equilibria or metastable states, different characteristic time scales, spatial patterns, order-disorder, could find an explanation in this picture.Comment: 11 pages, 8 figure

Hepatitis E Virus Antibodies in Patients with Chronic Liver Disease

In the United States, the seroprevalence rate for hepatitis E virus (HEV) is ≈20%. This study examined HEV seroprevalence in persons with and without chronic liver disease. Our data indicate that HEV seropositivity is high in patients with chronic liver disease and that HEV seroprevalence increases significantly with age

Correlations between climate network and relief data

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