Association between T2-related co-morbidities and effectiveness of biologics in severe asthma

Acknowledgments The authors thank Mr. Joash Tan (BSc, Hons), of the Observational and Pragmatic Research Institute (OPRI), and Ms Andrea Lim (BSc, Hons) of the Observational Pragmatic Research Institute (OPRI) for their editorial and formatting assistance that supported the development of this publication. Funding statement: This study was conducted by the Observational and Pragmatic Research Institute (OPRI) Pte Ltd and was partially funded by Optimum Patient Care Global and AstraZeneca Ltd. AstraZeneca UK LimitedPeer reviewe

La investigación formativa en ciencias empresariales: .Experiencias de investigación formativa POLIPIF

El material propuesto en el libro resume gran parte de la calidad de los trabajos presentados y la evolución en el desarrollo de las capacidades de los estudiantes en un contexto actual, complejo y retador, que refleja la realidad de las organizaciones actuales sobre escenarios estratégicos y manejo de situaciones complejas, para que, cuando lleguen a ser empleados o emprendedores, sepan afrontar cada paso hacia el cambio

A high-throughput and high peak capacity narrow-bore parallel segmented flow column strategy for the liquid chromatography-tandem mass spectrometry analysis of organic contaminants in water

This study highlights the development of a high peak capacity, high-throughput (HTP) approach for a target list of 62 organic contaminants in an environmental river water matrix on a HPLC conventional 400 bar system. Key separation metrics were evaluated: (a) peak capacity, (b) total analysis time and (c) mobile phase consumption. An average peak width of 0.10 min with a total analysis time (inclusive of the column wash and re-equilibration) of 10 minutes revealed the increased productivity and performance of the parallel segmented flow (PSF) column technology HPLC-MS strategy, comparable to UHPLC peak widths and analysis times, achieved at a significantly lower backpressure (<400 bar). The operation of the PSF in a narrow-bore scale format (internal diameter of 2.1 mm) resulted in a conservative HPLC scale total mobile phase consumption of 15 mL for the column separation; only 5.5 mL of this volume was exposed to the ion source per injection. Mobile phase consumption was higher compared to that of UHPLC, but on the other hand it achieved higher peak capacity. Three representative compounds (atrazine, diclofenac and fluazuron) with differing retention and ionisation properties were studied in detail in terms of detection sensitivity. The majority of the ion ratios for the standards in the river water matrix were within ±30% of the average ion ratios. The largest ion suppression occurred for atrazine (-25% matrix effect), a pesticide notorious for poor ionization and poor peak shape issues. The lowest response transitions at 1 μg L-1 for the extracted ions in the river water matrix of atrazine, diclofenac and fluazuron had signal to noise ratios ≥3, with the exception of diclofenac where 5 μg L-1 was the lowest calibration level. The peak area's calibration curve slope and standard deviation in the detector's response determination of limit of quantification (LOQ) were between 5.2 and 30.4 μg L-1

A parallel segmented flow column interface for microflow HPLC-MS separations for environmental organic contaminant analyses

Interfacing a microflow velocity < 100 μL/min analyses between the separation technique and MS detection for environmental organic contaminant analyses have shown potential to decrease the matrix effect. However performing such volumetric flow rates typically involves the upgrade of equipment. A plug and play study to demonstrate the potential of the Parallel Segmented Flow (PSF) column technology without any changes to the separation or detection programmed settings can facilitate microflow separations on a HPLC 400 bar hyphenated MS system. Here, we present the PSF column that interfaced 54 μL/min with electrospray tandem mass spectrometry (MS/MS) detection. Advantages of this alternative plug and play PSF microflow interfacing strategy include robust HPLC separation % RSD retention times ≤ 0.38% and mobile phase consumption comparable to UHPLC consumption rates of 9 mL per analysis. The calculated matrix effect for microflow PSF was representative of reduced ion suppression/enhancement (<±20%), with the exception of atrazine in river water which experienced medium suppression (<±30%). The %ME is not yet competitive with capillary scale columns. The instrument detection limits (IDLs) calculated takes into account the variability (%RSD) and amount reaching the detector. The PSF had lower IDLs (3.09–7.66 pg) compared to the conventional column (1.53–31.61 pg), with the exception of atrazine's peak height calculation. This communication focused on the microflow PSF operation strictly on conventional HPLC tandem MS system, without hardware upgrades, and applicability for robust organic contaminant separations in environmental matrices (soil and river water)

The parallel segmented flow column as an alternative front-end LC strategy for trace analyses

The parallel segmented flow (PSF) chromatography column was applied for the first time to trace residue analyses with mass spectral detection. Three problematic pesticides, notorious for poor peak shape and ionization issues were selected to illustrate the advantages and disadvantages of utilizing the PSF column as an alternative front-end separation interface for LC-MS approaches. Assessment of performance for each pesticide was based on a spiked lemon matrix prepared via a QuEChERS (quick, easy, cheap, rugged and safe) sample preparation approach. Comparisons were made between assays conducted using a conventional column and a PSF column (operated with a 40% segmentation ratio), each packed with identical stationary phases and of the same column format. Both columns were connected to the MS detector using the exact same connective tubing and electrospray (ESI) conditions. The primary advantage of the PSF column was that it enabled an increase in throughput by at least 2.5 times compared to the conventional HPLC column. At the same time, the volumetric flow to the MS was exactly the same for both the PSF column and the conventional column. Effectively there was no difference in the sensitivity provided by peak areas between the PSF and conventional columns: peak area LOQ values were comparable, 4–9 μg/kg for the PSF and 4–8 μg/kg for the conventional column. This is a vital requirement for food control methods to secure absence at default maximum residue limit (MRL) of 10 μg/kg (10 ppb) level. Since the efficiency of the PSF was superior to that of the conventional column, the peak width of eluting components was reduced by as much as 70% in the former compared to the peak widths on the latter. Hence, the PSF column provides greater peak capacity and this will enable faster analysis of complex samples

Transcatheter Tricuspid Valve Replacement: Illustrative Case Reports and Review of State-of-Art

Tricuspid regurgitation (TR) is one of the most common heart valve diseases, associated a with poor prognosis since significant TR is associated with an increased mortality risk compared to no TR or mild regurgitation. Surgery is the standard treatment for TR, although it is associated with high morbidity, mortality, and prolonged hospitalization, particularly in tricuspid reoperation after left-sided surgery. Thus, several innovative percutaneous transcatheter approaches for repair and replacement of the tricuspid valve have gathered significant momentum and have undergone extensive clinical development in recent years, with favorable clinical outcomes in terms of mortality and rehospitalization during the first year of follow-up. We present three clinical cases of transcatheter tricuspid valve replacement in an orthotopic position with two different innovative systems along with a review of the state-of-the-art of this emergent topic

Transcatheter Tricuspid Valve Replacement: Illustrative Case Reports and Review of State-of-Art

Tricuspid regurgitation (TR) is one of the most common heart valve diseases, associated a with poor prognosis since significant TR is associated with an increased mortality risk compared to no TR or mild regurgitation. Surgery is the standard treatment for TR, although it is associated with high morbidity, mortality, and prolonged hospitalization, particularly in tricuspid reoperation after left-sided surgery. Thus, several innovative percutaneous transcatheter approaches for repair and replacement of the tricuspid valve have gathered significant momentum and have undergone extensive clinical development in recent years, with favorable clinical outcomes in terms of mortality and rehospitalization during the first year of follow-up. We present three clinical cases of transcatheter tricuspid valve replacement in an orthotopic position with two different innovative systems along with a review of the state-of-the-art of this emergent topic

Investigación, desarrollo tecnológico e innovación en ingeniería y administración

El objetivo del libro es divulgar resultados de investigación producto de los proyectos de Investigación, desarrollo e innovación en las áreas de ingenierías y administración, ejecutados en las instituciones que hacen parte de la Red de Conocimiento en el sector de la Ingeniería–RECSI e instituciones aliadas nacionales e internacionales, y que han sido presentados en el marco del Encuentro Internacional en ciencias aplicadas e ingenierías EISI, entre los años 2018 y 2022. La obra consolida el trabajo de la red y de sus instituciones aliadas, en la generación y apropiación social del conocimiento en diferentes regiones tanto nacionales como internacionales. Se presenta en tres secciones; “Gestión, desarrollo y competitividad”, “Ingenierías y administración” y “Formación en ingenierías”.Creación del SENA versus archivos conservados -- En “Rescate y organización de los fondos documentales acumulados”: Testimonio de las Tablas de Valoración Documental TVD -- Primer soporte tecnológico para conservación documental y apoyo a eliminación del soporte papel: microfilmación -- Incidencia de la Ley General de Archivos, junio de 2000 en la gestión documental y administración de archivos del SENA -- La identificación archivística como línea de acción institucional - Experiencia en la elaboración de tablas de retención documental -- Incidencia de la Ley del Archivista en el Servicio Nacional de Aprendizaje SENA -- Segunda versión de instrumento archivístico: tabla de retención documental TRD -- Primer instrumento archivístico en el registro único de series y subseries documentales de la nación -- ON BASE - Primer Sistema de gestión documental SGD -- Plan de Mejoramiento Archivístico: Un reto para superar -- Diagnósticos Archivísticos: Base para la Planeación Estratégica de la Entidad -- Instrumentos Archivísticos: Herramientas claves para el desarrollo documental -- Adquisición e implementación del sistema de gestión de documentos electrónicos de archivo SGDEA - Para la conformación del archivo electrónico SENA - AESna243 página