A step forward in tropical anthracology: understanding woodland vegetation and wood uses in ancient Sri Lanka based on charcoal records from Mantai, Kirinda and Kantharodai

The aim of this study is to present the anthracological results from three archaeological sites located in the North, North West and South East of Sri Lanka. The study is based on the observation and analysis of 1689 charcoal fragments using for support the reference collection of South Indian wood at the Institute of Archaeology ( UCL), Inside Wood (2004-onwards) and several wood anatomy atlases. Mantai (200 BCE-850 CE), an urban site, has yielded 25 taxa with significant presence of cf. Cocos nucifera among other taxa. Kantharodai (400-170- BCE), an urban site, has yielded 19 taxa from arid zones (Fabaceae, Rubiaceae), mangroves (Rhizophoraceae) and dune zones (cf. Cocos nucifera). Kirinda (500–900 CE), a fishing settlement, has yielded 24 taxa including Fabaceae (Dalbergia, Acacia) and Rubiaceae, belonging to dry deciduous forest and open savannas. This collective data set allows for the identification of discernible patterns related to the use of ecological interfaces between the forest and the open plains, used and actively managed by humans, and the possibility to identify if this changed with an increase in maritime trade and/or changes in agriculture over time. This study provides evidence of the differences in the vegetation present as well as use of wood fuel and other specific uses of wood for each site examined. It also sheds new light on tropical anthracology regarding quantification and accuracy in taxa identification

Histidine switch controlling pH-dependent protein folding and DNA binding in a transcription factor at the core of synthetic network devices

© 2016 The Royal Society of Chemistry. Therapeutic strategies have been reported that depend on synthetic network devices in which a urate-sensing transcriptional regulator detects pathological levels of urate and triggers production or release of urate oxidase. The transcription factor involved, HucR, is a member of the multiple antibiotic resistance (MarR) protein family. We show that protonation of stacked histidine residues at the pivot point of long helices that form the scaffold of the dimer interface leads to reversible formation of a molten globule state and significantly attenuated DNA binding at physiological temperatures. We also show that binding of urate to symmetrical sites in each protein lobe is communicated via the dimer interface. This is the first demonstration of regulation of a MarR family transcription factor by pH-dependent interconversion between a molten globule and a compact folded state. Our data further suggest that HucR may be utilized in synthetic devices that depend on detection of pH changes

Diffusion and viscosity in a supercooled polydisperse system

We have carried out extensive molecular dynamics simulations of a supercooled polydisperse Lennard-Jones liquid with large variations in temperature at a fixed pressure. The particles in the system are considered to be polydisperse both in size and mass. The temperature dependence of the dynamical properties such as the viscosity ($\eta$) and the self-diffusion coefficients ($D_i$) of different size particles is studied. Both viscosity and diffusion coefficients show super-Arrhenius temperature dependence and fit well to the well-known Vogel-Fulcher-Tammann (VFT) equation. Within the temperature range investigated, the value of the Angell's fragility parameter (D $\approx 1.4$) classifies the present system into a strongly fragile liquid. The critical temperature for diffusion ($T_o^{D_i}$) increases with the size of the particles. The critical temperature for viscosity ($T_o^{\eta}$) is larger than that for the diffusion and a sizeable deviations appear for the smaller size particles implying a decoupling of translational diffusion from viscosity in deeply supercooled liquid. Indeed, the diffusion shows markedly non-Stokesian behavior at low temperatures where a highly nonlinear dependence on size is observed. An inspection of the trajectories of the particles shows that at low temperatures the motions of both the smallest and largest size particles are discontinuous (jump-type). However, the crossover from continuous Brownian to large length hopping motion takes place at shorter time scales for the smaller size particles.Comment: Revtex4, 7 pages, 8 figure

Kinetic Heterogeneities in a Highly Supercooled Liquid

We study a highly supercooled two-dimensional fluid mixture via molecular dynamics simulation. We follow bond breakage events among particle pairs, which occur on the scale of the $\alpha$ relaxation time $\tau_{\alpha}$. Large scale heterogeneities analogous to the critical fluctuations in Ising systems are found in the spatial distribution of bonds which are broken in a time interval with a width of order $0.05\tau_{\alpha}$. The structure factor of the broken bond density is well approximated by the Ornstein-Zernike form. The correlation length is of order $100 \sigma_1$ at the lowest temperature studied, $\sigma_1$ being the particle size. The weakly bonded regions thus identified evolve in time with strong spatial correlations.Comment: 3 pages, 6 figure

Deep 1.1 mm-wavelength imaging of the GOODS-South field by AzTEC/ASTE -- II. Redshift distribution and nature of the submillimetre galaxy population

We report the results of the counterpart identification and a detailed analysis of the physical properties of the 48 sources discovered in our deep 1.1mm wavelength imaging survey of the GOODS-South field using the AzTEC instrument on the Atacama Submillimeter Telescope Experiment (ASTE). One or more robust or tentative counterpart candidate is found for 27 and 14 AzTEC sources, respectively, by employing deep radio continuum, Spitzer MIPS & IRAC, and LABOCA 870 micron data. Five of the sources (10%) have two robust counterparts each, supporting the idea that these galaxies are strongly clustered and/or heavily confused. Photometric redshifts and star formation rates (SFRs) are derived by analyzing UV-to-optical and IR-to-radio SEDs. The median redshift of z~2.6 is similar to other earlier estimates, but we show that 80% of the AzTEC-GOODS sources are at z>2, with a significant high redshift tail (20% at z>3.3). Rest-frame UV and optical properties of AzTEC sources are extremely diverse, spanning 10 magnitude in the i- and K-band photometry with median values of i=25.3 and K=22.6 and a broad range of red colour (i-K=0-6). These AzTEC sources are some of the most luminous galaxies in the rest-frame optical bands at z>2, with inferred stellar masses of (1-30) x 10^{10} solar masses and UV-derived star formation rates of SFR(UV) > 10-1000 solar masses per year. The IR-derived SFR, 200-2000 solar masses per year, is independent of redshift or stellar mass. The resulting specific star formation rates, SSFR = 1-100 per Gyr, are 10-100 times higher than similar mass galaxies at z=0, and they extend the previously observed rapid rise in the SSFR with redshift to z=2-5. These galaxies have a SFR high enough to have built up their entire stellar mass within their Hubble time. We find only marginal evidence for an AGN contribution to the near-IR and mid-IR SEDs. (abridged)Comment: 31 pages including 14 figures, accepted for publication in the MNRAS. A higher quality Figure 1 is also included as Figure1.jp

Random Walks on a Fluctuating Lattice: A Renormalization Group Approach Applied in One Dimension

We study the problem of a random walk on a lattice in which bonds connecting nearest neighbor sites open and close randomly in time, a situation often encountered in fluctuating media. We present a simple renormalization group technique to solve for the effective diffusive behavior at long times. For one-dimensional lattices we obtain better quantitative agreement with simulation data than earlier effective medium results. Our technique works in principle in any dimension, although the amount of computation required rises with dimensionality of the lattice.Comment: PostScript file including 2 figures, total 15 pages, 8 other figures obtainable by mail from D.L. Stei

Constraining the optical emission from the double pulsar system J0737-3039

We present the first optical observations of the unique system J0737-3039 (composed of two pulsars, hereafter PSR-A and PSR-B). Ultra-deep optical observations, performed with the High Resolution Camera of the Advanced Camera for Surveys on board the Hubble Space Telescope could not detect any optical emission from the system down to m_F435W=27.0 and m_F606W=28.3. The estimated optical flux limits are used to constrain the three-component (two thermal and one non-thermal) model recently proposed to reproduce the XMM-Newton X-ray spectrum. They suggest the presence of a break at low energies in the non-thermal power law component of PSR-A and are compatible with the expected black-body emission from the PSR-B surface. The corresponding efficiency of the optical emission from PSR-A's magnetosphere would be comparable to that of other Myr-old pulsars, thus suggesting that this parameter may not dramatically evolve over a time-scale of a few Myr.Comment: 16 pages, 3 figures, ApJ accepte

Protein-coding and non-coding gene expression analysis in differentiating human keratinocytes using a three-dimensional epidermal equivalent

The epidermal compartment is complex and organized into several strata composed of keratinocytes (KCs), including basal, spinous, granular, and corniWed layers. The continuous process of self-renewal and barrier formation is dependent on a homeostatic balance achieved amongst KCs involving proliferation, diVerentiation, and cell death. To determine genes responsible for initiating and maintaining a corniWed epidermis, organotypic cultures comprised entirely of stratiWed KCs creating epidermal equivalents (EE) were raised from a submerged state to an air/liquid (A/L) interface. Compared to the array proWle of submerged cultures containing KCs predominantly in a proliferative (relatively undiVerentiated) state, EEs raised to an A/L interface displayed a remarkably consistent and distinct proWle of mRNAs. Cultures lifted to an A/L interface triggered the induction of gene groups that regulate proliferation, diVerentiation, and cell death. Next, diVerentially expressed microRNAs (miRNAs) and long noncoding (lncRNA) RNAs were identiWed in EEs. Several diVerentially expressed miRNAs were validated by qRT-PCR and Northern blots. miRNAs 203, 205 and Let-7b were up-regulated at early time points (6, 18 and 24 h) but downregulated by 120 h. To study the lncRNA regulation in EEs, we proWled lncRNA expression by microarray and validated the results by qRT-PCR. Although the diVerential expression of several lncRNAs is suggestive of a role in epidermal diVerentiation, their biological functions remain to be elucidated. The current studies lay the foundation for relevant model systems to address such fundamentally important biological aspects of epidermal structure and function in normal and diseased human skin

Normalization factors for magnetic relaxation of small particle systems in non-zero magnetic field

We critically discuss relaxation experiments in magnetic systems that can be characterized in terms of an energy barrier distribution, showing that proper normalization of the relaxation data is needed whenever curves corresponding to different temperatures are to be compared. We show how these normalization factors can be obtained from experimental data by using the $T \ln(t/\tau_0)$ scaling method without making any assumptions about the nature of the energy barrier distribution. The validity of the procedure is tested using a ferrofluid of Fe_3O_4 particles.Comment: 5 pages, 6 eps figures added in April 22, to be published in Phys. Rev. B 55 (1 April 1997