The coagulation system helps control infection caused by the ciliate parasite Philasterides dicentrarchi in the turbot Scophthalmus maximus (L.)

This is the accepted manuscript of the following article: Blanco-Abad, V., Noia, M., Valle, A., Fontenla, F., Folgueira, I., & De Felipe, A. et al. (2018). The coagulation system helps control infection caused by the ciliate parasite Philasterides dicentrarchi in the turbot Scophthalmus maximus (L.). Developmental & Comparative Immunology, 87, 147-156. doi: 10.1016/j.dci.2018.06.001Many studies have shown that coagulation systems play an important role in the defence against pathogens in invertebrates and vertebrates. In vertebrates, particularly in mammals, it has been established that the coagulation system participates in the entrapment of pathogens and activation of the early immune response. However, functional studies investigating the importance of the fish coagulation system in host defence against pathogens are scarce. In the present study, injection of turbot (Scopthalamus maximus) with the pathogenic ciliate Philasterides dicentrarchi led to the formation of macroscopic intraperitoneal clots in the fish. The clots contained abundant, immobilized ciliates, many of which were lysed. We demonstrated that the plasma clots immobilize and kill the ciliates in vitro. To test the importance of plasma clotting in ciliate killing, we inhibited the process by adding a tetrapeptide known to inhibit fibrinogen/thrombin clotting in mammals. Plasma tended to kill P. dicentrarchi slightly faster when clotting was inhibited by the tetrapeptide, although the total mortality of ciliates was similar. We also found that kaolin, a particulate activator of the intrinsic pathway in mammals, accelerates plasma clotting in turbot. In addition, PMA-stimulated neutrophils, living ciliates and several ciliate components such as cilia, proteases and DNA also displayed procoagulant activity in vitro. Injection of fish with the ciliates generated the massive release of neutrophils to the peritoneal cavity, with formation of large aggregates in those fish with live ciliates in the peritoneum. We observed, by SEM, numerous fibrin-like fibres in the peritoneal exudate, many of which were associated with peritoneal leukocytes and ciliates. Expression of the CD18/CD11b gene, an integrin associated with cell adhesion and the induction of fibrin formation, was upregulated in the peritoneal leukocytes. In conclusion, the findings of the present study show that P. dicentrarchi induces the formation of plasma clots and that the fish coagulation system may play an important role in immobilizing and killing this parasiteThis work was financially supported by the European Union’s Horizon 2020 research and innovation programme under grant agreement No. 634429 (PARAFISHCONTROL), by the Ministerio de Economía y Competitividad (Spain) under grant agreement AGL2014-57125-R and by grant GPC2014/069 from the Xunta de Galicia (Spain)S

Epidemia de Gripe A en Buenos Aires en 2009 y la comunicación de la prevención colectiva

Objetivo: Analizar longitudinalmente la construcción de la participación social en la prevención de la gripe A en 2009, evaluando sincrónicamente discursos sobre prevención y evolución de la epidemia en clase media urbana de Buenos Aires. Métodos: Triangulación cuali-cuantitativa: 1) Evolución de enfermedad tipo Influenza en un Hospital de Comunidad que atiende clase media porteña; 2) Evolución de casos a nivel país; 3) Representación de la prevención en medios gráficos nacionales. Resultados: El brote epidémico en clase media porteña precedió al total país, período en el que el porcentaje en tapa de notas sobre gripe A era 3,9%. Las medidas que previenen colectivamente la difusión viral (higiene de manos, superficies y contención de estornudos) se comunicaron posteriormente al pico epidémico. Conclusiones: La comunicación contribuyó a construir tardíamente la participación social. Esto evidencia la necesidad de promover la participación temprana de la sociedad en la prevención colectiva frente a riesgo epidémicos