Tailoring the partitioning of proteins using ionic liquids as adjuvants in polymer-polymer aqueous biphasic systems

Aqueous biphasic systems (ABS) are promising for proteins purification; however, when dealing with samples comprising several proteins, the selectivity towards a target protein is difficult to achieve. In this work, the addition of ionic liquids (ILs) as adjuvants (5 and 10 wt%) in ABS composed of polyethyleneglycol (PEG) and Dextran was investigated to tailor proteins’ partitioning between the coexisting phases. The liquid-liquid phase diagrams were determined at 298 K, followed by partition studies of three proteins (bovine serum albumin (BSA), immunoglobulin G (IgG), and cytochrome C (Cyt C)). Partition coefficients of IgG and BSA indicate the preference of both proteins to the dextran-rich phase, whereas Cyt C has no preferential partitioning between the phases. The addition of chloride-based ILs as adjuvants allows to increase or decrease the partition coefficients, thus tailoring the proteins partitioning between the phases. BSA partitioning essentially depends on the IL content in each phase, whereas Cyt C and IgG partitioning is ruled by the ILs chemical structure and established interactions. Molecular docking was carried out to address the ILs effect on the proteins partitioning, supporting experimental observations, while identifying the specific interactions occurring. The partition of each protein in polymer-salt ABS with ILs as adjuvants was determined, demonstrating the higher tailoring ability of polymer-polymer ABS when adding ILs. Finally, the partition of each protein in presence of the remaining ones was determined, as well as the selectivity of the studied systems to separate each pair of proteins, paving the way for their use in liquid-liquid chromatography.publishe

Influência da profundidade de semeio na germinação de sementes de mulungu (Erythrina vellutina Wild - Leguminosae).

O objetivo desse trabalho foi analisar a profundidade na germinação, avaliando-se o número médio de dias requeridos para a primeira emergência, a porcentagem de germinação (G) e o Índice de Velocidade de Emergência (IVE)

Influência da posição de semeio na germinação de sementes de mulungu (Erythrina vellutina Wild.).

O objetivo desse trabalho foi analisar os efeitos da posição de sementes na sua germinação, visando estabelecer recomendação técnica para a produção de mudas desta espécie

Metabolic syndrome in patients with chronic hepatitis C virus genotype 1 infection who do not have obesity or type 2 diabetes

OBJECTIVE: The individual components of metabolic syndrome may be independent predictors of mortality in patients with liver disease. We aimed to evaluate the prevalence of metabolic syndrome and its related components in hepatitis C virus-infected patients who are not obese and do not have type 2 diabetes. METHODS: This cross-sectional study included 125 patients infected with hepatitis C virus genotype 1. Metabolic syndrome was defined according to the International Diabetes Federation. Anthropometric data were measured according to standardized procedures. Bioimpedance analysis was performed on all patients. RESULTS: Metabolic syndrome was diagnosed in 21.6&#37; of patients. Of the subjects with metabolic syndrome, 59.3&#37; had hypertension, 77.8&#37; had insulin resistance, 85.2&#37; were overweight, 48.1&#37; had a high waist circumference, 85.2&#37; had an increased body fat percentage, and 92.3&#37; had an elevated waist:hip ratio. In the bivariate analysis, female sex (OR 2.58; 95&#37; CI: 1.09-6.25), elevated gamma-glutamyl transferase (&#947;GT) (OR 2.63; 95&#37; CI: 1.04-7.29), elevated fasting glucose (OR 8.05; 95&#37; CI: 3.17-21.32), low HDL cholesterol (OR 2.80; 95&#37; CI: 1.07-7.16), hypertriglyceridemia (OR 7.91; 95&#37; CI: 2.88-22.71), elevated waist circumference (OR 10.33; 95&#37; CI: 3.72-30.67), overweight (OR 11.33; 95&#37; CI: 3.97-41.07), and increased body fat percentage (OR 8.34; 95&#37; CI: 2.94-30.08) were independent determinants of metabolic syndrome. Using the final multivariate regression model, similar results were observed for abdominal fat (OR 9.98; 95&#37; CI: 2.63-44.41) and total body fat percentage (OR 8.73; 95&#37; CI: 2.33-42.34). However, metabolic syndrome risk was also high for those with blood glucose >5.55 mmol/L or HDL cholesterol <0.9 mmol/L (OR 16.69; 95&#37; CI: 4.64-76.35; OR 7.23; 95&#37; CI: 1.86-32.63, respectively). CONCLUSION: Metabolic syndrome is highly prevalent among hepatitis C virus-infected patients without type 2 diabetes or obesity. Metabolic syndrome was significantly associated with hypertension, insulin resistance, increased abdominal fat, and overweight

Agricultura familiar e agroindústria canavieira: impasses sociais.

A Política Agroenergética para produção de biocombustíveis é apresentada sob os pressupostos do desenvolvimento sustentável. O álcool, produzido a partir da cana-de-açúcar, aparece como a alternativa mais viável, o que estimula a expansão e redistribuição espacial da produção canavieira. O estado de Goiás está entre as principais rotas de ampliação da cana. O objetivo do artigo é compreender o debate das questões sociais no plano dos discursos oficiais em torno do Plano Nacional de Agroenergia, bem como as contradições sociais associadas à expansão da monocultura canavieira em regiões de predomínio da agricultura familiar. O desenvolvimento do tema baseou-se num estudo de caso, realizado no município de Itapuranga (GO), valendo-se de uma abordagem qualitativa e da combinação das técnicas de pesquisa bibliográfica, observação e, principalmente, entrevistas. Constatou-se que a expansão do cultivo da cana-de-açúcar contribuiu para transformar o contexto social de Itapuranga e provocar alterações consideráveis na dinâmica produtiva e organizacional dos agricultores familiares

Peso ao nascer em bubalinos no Estado de Rondônia.

O presente estudo objetivou avaliar o peso ao nascer de bezerros bubalinos de diferentes composições genéticas e determinar os possíveis efeitos de meio e de herança que causam variações sobre ele. O peso ao nascer médio ajustado foi de 34,25 ± 5,02. O sexo do bezerro ocasionou variações significativas (P0,05) sobre esta característica. A herdabilidade para o peso ao nascer de bubalinos mestiços, estimada no presente estudo, foi considerada alta, apresentando valor de 0,60 ± 0,12.bitstream/CPAF-RO-2010/13268/1/bpd54-bubalinos.pd

Intervalo de partos em rebanho bubalino no Estado de Rondônia.

O presente estudo objetivou avaliar o intervalo de partos de búfalas, em diferentes composições genéticas Murrah x Mediterrâneo e determinar os possíveis efeitos de ambiente que causam variações sobre elas. A média para o intervalo de partos foi de 451±139 dias. A variável grupo contemporâneo da búfala, em função do ano e época de ocorrência do parto precedente, as covariáveis peso da búfala pós-parto anterior e idade da búfala ao parto anterior, linear e quadrática, bem como o efeito aleatório de búfala, exerceram influência (P0,05) sobre o intervalo de partos.bitstream/CPAF-RO-2010/13265/1/bpd53-bubalinos-.pd

East Asia Today

East Asia attracts growing interest in the West. Th e region is the world’s hotbed of economic growth, led by burgeoning China aided by more advanced Asian economies investing heavily in manufacturing and trading networks involving China. Western entrepreneurs clamor to join the China wave

Hippocampal Astrocytes in Migrating and Wintering Semipalmated Sandpiper

Seasonal migratory birds return to the same breeding and wintering grounds year after year, and migratory long-distance shorebirds are good examples of this. These tasks require learning and long-term spatial memory abilities that are integrated into a navigational system for repeatedly locating breeding, wintering, and stopover sites. Previous investigations focused on the neurobiological basis of hippocampal plasticity and numerical estimates of hippocampal neurogenesis in birds but only a few studies investigated potential contributions of glial cells to hippocampal-dependent tasks related to migration. Here we hypothesized that the astrocytes of migrating and wintering birds may exhibit significant morphological and numerical differences connected to the long-distance flight. We used as a model the semipalmated sandpipe