The Nup84 complex coordinates the DNA damage response to warrant genome integrity

DNA lesions interfere with cellular processes such as transcription and replication and need to be adequately resolved to warrant genome integrity. Beyond their primary role in molecule transport, nuclear pore complexes (NPCs) function in other processes such as transcription, nuclear organization and DNA double strand break (DSB) repair. Here we found that the removal of UV-induced DNA lesions by nucleotide excision repair (NER) is compromised in the absence of the Nup84 nuclear pore component. Importantly, nup84Δ cells show an exacerbated sensitivity to UV in early S phase and delayed replication fork progression, suggesting that unrepaired spontaneous DNA lesions persist during S phase. In addition, nup84Δ cells are defective in the repair of replication-born DSBs by sister chromatid recombination (SCR) and rely on post-replicative repair functions for normal proliferation, indicating dysfunctions in the cellular pathways that enable replication on damaged DNA templates. Altogether, our data reveal a central role of the NPC in the DNA damage response to facilitate replication progression through damaged DNA templates by promoting efficient NER and SCR and preventing chromosomal rearrangements.European Research Council ERC2014 AdG669898 TARLOOPMinisterio de Economía y Competitividad BFU2016-75058-PJunta de Andalucía PA12-BIO123

Transparency Policies in European Public Broadcasters: Sustainability, Digitalisation and Fact-Checking

Over the last few years, European public broadcasters have promoted the concept of public service media as one of their main values. To this end, transparency policies have been implemented as a mechanism of corporate projection by strengthening their role as an essential service. The objective of this article is to ascertain the existence of this type of policies among European public broadcasters. To this end, a nominal group was made with 24 experts who were surveyed, thus generating new indicators of transparency and accountability strategies around sustainability and digitalization. The contents of the websites of RTVE (Spain), RTP (Portugal), France TV (France), RAI (Italy), BBC (UK), RTÉ (Ireland), ZDF (Germany), VRT (Belgium), and SVT (Sweden) were also analyzed, paying attention to such indicators and strategies. The main results include the identification of differences on the basis of the ideal models described by Hallin and Mancini; a commitment to credibility (fact-checking) to the detriment of diversity of opinions; and a connection between the political system and the media system, which, preliminarily, determines the level of transparency of these public entitiesThe results of this research are part of the work of the “Equipo de Investigaciones Políticas” (USC). It is also part of the project “Nuevos valores, gobernanza, financiación y servicios audiovisuales públicos para la sociedad de Internet: contrastes europeos y españoles” (2019–2021) financed by the Spanish Ministry of Innovation and Universities (RTI2018-096065-B-I00)S

Efecto inhibitorio de chara braunii Gmelin en el crecimiento poblacional de microcystis aeruginosa

Esta investigación tuvo como objetivo analizar los efectos inhibitorios de Chara Braunii sobre el crecimiento poblacional de Microcystis aeruginosa. Para el logro de los objetivos específicos, 1) se realizaron colectas e identificación taxonómica de las especies, utilizando claves de identificación. Posteriormente, el aislamiento y cultivo de Microcystis aeruginosa utilizando 6 biorreactores y medio BG11 en dosificación de 2000 mL, dando como resultado que las condiciones de crecimiento ideales fueron temperatura promedio de 25 ± 3°C, PAR de 0,8086 µmol•s⁻¹•m⁻², fotoperiodo de 14:10, pH de 7,10 y agitación diaria. Con los datos de densidad, se aplicó el modelo Lokta-Volterra, los resultados indicaron que las estaciones de primavera y verano, se registró exclusión competitiva de Microcystis aeruginosa siendo Chara Braunii la de mayor competitividad espacial. 2) Por HPLC se determinaron los ácidos grasos de Chara Braunii, siendo los ácidos oleico, linoléico, palmítico y esteárico los de mayor porcentaje. 3) Se realizaron extractos de la macroalga, que fueron aplicadas al cultivo de la cianobacteria en dosificaciones de 3,5, 15 y 20 mL, calculando diariamente la concentración celular y rango inhibitorio. Se aplicó el análisis de varianza y comparación de medias por el método Fisher con lo cual se determinó que hubo una reducción de la concentración celular al 5to día de experimentación y una mayor inhibición de Microcystis aeruginosa aplicando la concentración de 20 mL del extracto, infiriendo que los compuestos alelopáticos de Chara Braunii podrían ser los ácidos grasos presentes en la planta. Se concluye que a mayor concentración del extracto de la macroalga, esta inhibe a Microcystis aeruginosa, siendo posible su aplicación en lagos u embalses que presenten floraciones algales de este tipo.CONACYT - Consejo Nacional de Ciencia y TecnologíaPROCIENCI

Excess of Yra1 RNA-Binding Factor Causes Transcription-Dependent Genome Instability, Replication Impairment and Telomere Shortening

Yra1 is an essential nuclear factor of the evolutionarily conserved family of hnRNP-like export factors that when overexpressed impairs mRNA export and cell growth. To investigate further the relevance of proper Yra1 stoichiometry in the cell, we overexpressed Yra1 by transforming yeast cells with YRA1 intron-less constructs and analyzed its effect on gene expression and genome integrity. We found that YRA1 overexpression induces DNA damage and leads to a transcription-associated hyperrecombination phenotype that is mediated by RNA:DNA hybrids. In addition, it confers a genome-wide replication retardation as seen by reduced BrdU incorporation and accumulation of the Rrm3 helicase. In addition, YRA1 overexpression causes a cell senescence-like phenotype and telomere shortening. ChIP-chip analysis shows that overexpressed Yra1 is loaded to transcribed chromatin along the genome and to Y’ telomeric regions, where Rrm3 is also accumulated, suggesting an impairment of telomere replication. Our work not only demonstrates that a proper stoichiometry of the Yra1 mRNA binding and export factor is required to maintain genome integrity and telomere homeostasis, but suggests that the cellular imbalance between transcribed RNA and specific RNA-binding factors may become a major cause of genome instability mediated by co-transcriptional replication impairment.España Ministerio de Ciencia e Innovación BFU2010-16372, BFU2013-42918Andalucía, Junta de Andalucía BIO102, CVI4567 and BIO123

A Follow-Up Study of Cognitive Development in Low Risk Preterm Children

[Abstract] The results of a longitudinal study on the cognitive development of one group of full-term and three groups of low risk preterm children with different gestational ages (GA) are presented. The 181 participants were divided into four GA groups of similar size. The aims were: 1) To check if there are differences in cognitive development (measured through the Batelle scale) among the GA groups. 2) To establish the predictive factors of cognitive development at 22 and 60 months of age, taking into account biomedical, environmental and individual factors. The results of the repeated measures ANOVA performed at 22 and 60 months of age indicated that the cognitive trajectories of the four GA groups were similar. Linear regression analyses showed that the effect of the different predictors changed in relation to the time of measurement of cognitive development. Biological factors and the quality of home environment had a moderate effect on the cognitive development at 22 months of age. Cognitive results obtained at 22 months of age, and, to a lesser extent, working memory had the greatest effect on cognitive development at 60 months. GA does not predict cognitive development. Preterm children do not show cognitive delay if they are healthy.This research was funded by the Ministerio de Ciencia e Innovación of the Spanish Government, grants number PSI2008-03905, PSI2011-23210 and PSI2015-66697-R to the first autho

Con Xosé Luís Méndez Ferrín en Vigo

Na mañá do 31 de decembro de 2010 reunímonos en Vigo con Xosé Luís Méndez Ferrín para falar sobre poesía. Ao longo da conversa tivemos ocasión de coñecer o seu parecer sobre a función que a poesía desempeña hoxe en día na sociedade, de percorrer a súa propia traxectoria poética, de comentar os seus gustos en canto lector e a súa visión do panorama da creación actual

A Follow-Up Study of Cognitive Development in Low Risk Preterm Children

The results of a longitudinal study on the cognitive development of one group of full-term and three groups of low risk preterm children with different gestational ages (GA) are presented. The 181 participants were divided into four GA groups of similar size. The aims were: 1) To check if there are differences in cognitive development (measured through the Batelle scale) among the GA groups. 2) To establish the predictive factors of cognitive development at 22 and 60 months of age, taking into account biomedical, environmental and individual factors. The results of the repeated measures ANOVA performed at 22 and 60 months of age indicated that the cognitive trajectories of the four GA groups were similar. Linear regression analyses showed that the effect of the different predictors changed in relation to the time of measurement of cognitive development. Biological factors and the quality of home environment had a moderate effect on the cognitive development at 22 months of age. Cognitive results obtained at 22 months of age, and, to a lesser extent, working memory had the greatest effect on cognitive development at 60 months. GA does not predict cognitive development. Preterm children do not show cognitive delay if they are healthyThis research was funded by the Ministerio de Ciencia e Innovación of the Spanish Government, grants number PSI2008-03905, PSI2011-23210 and PSI2015-66697-R to the first authorS

Studies of the Microbiological and Physico-Chemical Composition of Goat ́s Milk from North-Western Spain

P. 39-44Twenty-seven samples of raw goat’s milk were collected from four farms in the North-West of Spain with the aim of analysing counts of various mesophilic bacteria and of determining the physical and chemical composition during different seasons of the year. Overall mean values for mesophilic aerobic micro-organisms (Standard Plate Counts, SPCs) were above 5.6 log cfu/ml, very similar to the counts on M17 agar (presumptive Lactococcus, 5.6 log cfu/ml) and higher than the counts on MSE agar (presumptive Leuconostoc, 4.6 log cfu/ml) and on MRS agar (presumptive Lactobacillus, 4.4 log cfu/ml). Counts were generally higher in winter than in spring and summer, although no statistically significant differences were found. The microbiological quality of raw goat’s milk produced in the farms investigated in the North-West of Spain is only partially in accordance with European Union standards. Hence, hygiene practices should be improved so as to decrease the standard plate counts on some of the farms checked. The physical and chemical composition tests also showed higher values for fat, protein and dry extract during the winter season. The average fat content (5.5 g/100 ml) and dry extract (14.7 g/100 ml) were higher than the values noted in other studies, making this of interest for cheese makingS

Myiasis in the neotropical amphibian hypsiboas caingua (anura: hylidae) by megaselia scalaris (diptera: phoridae)

Host-parasite interactions between dipterans and anurans in the Neotropical region are still poorly known;we report here the first case of myiasis in the anuran Hypsiboas caingua (Hylidae). Three infecting larvae completedmetamorphosis in laboratory conditions. The emerging three adult flies were morphologically identified as Megaseliascalaris (Phoridae). This is the first evidence of phorid flies parasitizing a South American wild anuranFil: López, Carlos Ariel. Ministerio de Salud. Instituto Nacional de Medicina Tropical; ArgentinaFil: Lavinscky Pereira, Thalles Platiny. Universidade de Sao Paulo; BrasilFil: Antúnez, Marcio Germán. Ministerio de Salud. Instituto Nacional de Medicina Tropical; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; ArgentinaFil: Peichoto, María Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Ministerio de Salud. Instituto Nacional de Medicina Tropical; Argentin