Comportamento de frangos de corte em sistemas de aquecimento.

bitstream/item/58329/1/CUsersPiazzonDocuments274.pd

Predictive models for the phase behaviour and solution properties of weak electrolytes: nitric, sulfuric and carbonic acid

The distribution of ionic species in electrolyte systems is important in many fields of science and engineering, ranging from the study of degradation mechanisms to the design of systems for electrochemical energy storage. Often, other phenomena closely related to the ionic speciation, such as ion pairing, clustering and hydrogen bonding, which are difficult to investigate experimentally, are also of interest. Here, we develop an accurate molecular approach, accounting for reactions as well as association and ion pairing, to deliver a predictive framework that helps validate experiment and guides future modelling of speciation phenomena of weak electrolytes. We extend the SAFT-VRE Mie equation of state [D. K. Eriksen et al., Mol. Phys., 2016, 114, 2724–2749] to study aqueous solutions of nitric, sulphuric and carbonic acid, considering complete and partially dissociated models. In order to incorporate the dissociation equilibria, correlations to experimental data for the relevant thermodynamic equilibrium constants of the dissociation reactions are taken from the literature and are imposed as a boundary condition in the calculations. The models for water, the hydronium ion, and carbon dioxide are treated as transferable and are taken from our previous work. Here we present new molecular models for nitric acid, and the nitrate, bisulfate, sulfate, and bicarbonate anions. The resulting framework is used to predict a range of phase behaviour and solution properties of the aqueous acids over wide ranges of concentration and temperature, including the degree of dissociation, as well as the activity coefficients of the ionic species, and the activity of water and osmotic coefficient, density, and vapour pressure of the solutions. The SAFT-VRE Mie models obtained in this manner provide a means of elucidating the mechanisms of association and ion pairing in the systems studied, complementing the experimental observations reported in the literature

Comparative analysis of the biosimilar and innovative G-CSF modulated pathways on umbilical cord blood–derived mononuclear cells

Biosimilars of granulocyte colony-stimulating factor (G-CSF) have been routinely introduced into clinical practice. However, not functional genomics characterization has been performed yet in comparison with the innovator G-CSF. This study aimed to evaluate the transcriptomic changes in an in vitro model of umbilical cord blood cells (UBC) exposed to G-CSF for the identification of their modulated pathways. Umbilical cord blood cells–derived mononuclear cells (MNCs) were treated with biosimilar and innovator G-CSF for further gene expression profiling analysis using a microarray-based platform. Comparative analysis of biosimilar and innovator G-CSF gene expression signatures allowed us to identify the most commonly modulated pathways by both drugs. In brief, we observed predominantly upmodulation of transcripts related to PI3K-Akt, NF-kappaB, and tumor necrosis factor (TNF) signaling pathways as well as transcripts related to negative regulation of apoptotic process among others. In addition, hematopoietic colony-forming cell assays corroborate the G-CSF phenotypic effects over UBC-derived MNCs. In conclusion, our study suggests that G-CSF impacts UBC-derived cells through the modulation of several signaling pathways associated with cell survival, migration, and proliferation. The concordance observed between biosimilar and innovator G-CSF emphasizes their similarity in regards to their specificity and biological responses.Fil: Ávila Portillo, Luz Mabel. Universidad Nacional de Colombia; Colombia. Stem Medicina Regenerativa/CryoHoldco; Colombia. Hospital Militar Central. Unidad de Investigación; ColombiaFil: Aristizabal, F.. Universidad Nacional de Colombia; ColombiaFil: Perdomo, S.. Universidad El Bosque. Facultad de Odontología; ColombiaFil: Riveros, A.. Stem Medicina Regenerativa/CryoHoldco; ColombiaFil: Ospino, B.. Stem Medicina Regenerativa/CryoHoldco; ColombiaFil: Avila, J. P.. Stem Medicina Regenerativa/CryoHoldco; ColombiaFil: Butti, M.. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Investigaciones Inmunológicas Básicas y Aplicadas; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; ArgentinaFil: Abba, Martín Carlos. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Investigaciones Inmunológicas Básicas y Aplicadas; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentin

Recurso humano de enfermería según grado de dependencia de los pacientes

Quality of care in hospitals mainly depends on an effective use of resources, especially the staff. In the case of nursing, it is important to establish the nurse-patient ratio in order to optimise the quality of care. Thus, nursing staff must be aware of the conditions of the patients in their care, in order to determine both how many members of staff are required and the preparation needed. A quantitative, descriptive and cross-sectional study was carried out. The sample consisted of 98 patients, selected through non-probabilistic sampling for convenience. The information was collected over a period of two months using 'Test Delta' which determined the degree of dependence. The statistical package SPSS V20.00 was used for the analysis. The study identified four levels of dependence: no assistance required (22.4%), minor assistance required (28.6%), moderate assistance required (22.4%) and substantial assistance required (26.5%). Once categorised, the nursing staff demand was calculated. It was estimated that thirteen nursing professionals are required to satisfy the demand for care. The data reported shows that the majority of staff should be assigned to patients in the 'substantial assistance required' category. These results lead to the conclusion that it is important to develop methodologies to efficiently determine and assign nursing staff, taking into account the level of dependency as this is closely linked with the best outcome in patients. La calidad de la atención dentro de las organizaciones hospitalarias depende principalmente de la buena utilización de los recursos, especialmente el recurso humano; para enfermería resulta importante determinar la razón enfermera – paciente, con el fin de optimizar la calidad del cuidado, por ello, el profesional debe conocer las condiciones de los pacientes a su cargo, de tal manera que pueda definir cuanto personal requiere y su preparación. Se utilizó un estudio cuantitativo, descriptivo y transversal. La muestra fue de 98 pacientes, seleccionados a través del muestreo no probabilístico por conveniencia, la información se recolectó durante dos meses, mediante el instrumento “Test Delta” que determinó el grado de dependencia. Para el análisis se utilizó el paquete estadístico SPSS V20.00. Se identificaron cuatro categorías de dependencia: 22,4% válidos, 28,6% asistido leve, 22,4% asistido moderado y 26,5% asistido severo. Una vez categorizados, se calculó el recurso humano de enfermería, se estimó una dotación de trece profesionales para satisfacer las demandas de cuidado, los datos reportados muestran que la mayor parte del personal debe ser asignado a los pacientes en la categoría asistidos severos. Esto permite concluir que es importante desarrollar metodologías para determinar y asignar de forma eficiente, el recurso humano de enfermería, teniendo en cuenta el grado de dependencia, dado que está relacionado con mejores resultados en los pacientes

Detección y cuantificación de virus dengue 2 en lisado celular y plasma de niños por qPCR en tiempo real usando un estuche comercial y el equipo EcoTM System-Illumina

Methods  for  Dengue  virus  (DENV)  diagnosis  in  endemic areas  are  greatly  needed.  One of  them  is  the  real-time polymerase  chain  reaction  (qPCR)  that  also  enables  to quantitate  the  viral  genome.  Kits of  qPCR  for  DENV  are expensive and restrict their use to a small number of qPCR devices, which limits the application of the technique. Here, we evaluated the performance of a commercial kit of qPCR to DENV-2 detection  on  a  locally  available  qPCR  device (EcoTM System, Illumina), not cited by the kit manufacturer.VERO-76 cells lysate and plasma from children, both with confirmed ongoing DENV-2 infection, were evaluated. As specificity control, cell lysates and plasma  from  children infected  with  DENV-1,  and  uninfected  lysate,  were  also included. The reactions were simultaneously evaluated in an Applied Bio systems  7300  device.  The standard  curve generated  by  EcoTM  was  robust  (R2=  0.99)  with  low variability in the replicates (<10%). The reaction efficiency was high (88.8%) and signal was only obtained in lysates and plasma infected  with  DENV-2.  There was  a  strong positive  correlation  (R2=  1.0,  P=  0.0028)  between  the number of copies of viral RNA in the samples detected by both  qPCR  devices.  Thus, the use  of  the  evaluate  kit  for detection of DENV-2 here tested can be extended to EcoTM. With  this  work,  technological  capacity  for  DENV  study  in an endemic zone is greatly strengthened.Métodos para diagnóstico de dengue virus (DENV) en zonas endémicas son altamente necesarios. Uno de ellos es la reacción en cadena de polimerasa en tiempo real (qPCR), método que permite además la cuantificación del genoma viral. Los estuches comerciales de qPCR para DENV son costosos y restringen su uso a un número pequeño de dispositivos de qPCR, limitando la aplicación de la técnica. Aquí se evaluó el desempeño de un estuche comercial de qPCR para la detección de DENV-2 en un dispositivo de qPCR (EcoTM System, Illumina) localmente disponible, no listado por el fabricante del estuche. Lisado de células VERO-76 y plasma de niños, ambos con infección confirmada por DENV-2, fueron evaluados. Como controles de especificidad, lisado celular y plasma de niños infectados con DENV-1, además de lisado no infectado, fueron también incluidos. Las reacciones fueron además evaluadas simultáneamente en un equipo Applied Biosystem 7300, uno de los recomendados por el fabricante del estuche. La curva estándar generada por el EcoTM fue robusta (R2= 0.99), con baja variabilidad en las réplicas (<10%). La eficiencia de la reacción fue buena (88.8%) y sólo hubo amplificación en los lisados y plasma de niños infectados con DENV-2. Hubo una fuerte correlación positiva (R2= 1.0, P=0.0028) entre el número de copias de ARN viral en las muestras detectadas por los dos dispositivos de qPCR usados. Así, el uso del estuche para detección de DENV-2 aquí probado puede extenderse al EcoTM de forma segura. Este trabajo fortalece la capacidad tecnológica para el estudio de DENV en un área endémica

HERQ-9 Is a New Multiplex PCR for Differentiation and Quantification of All Nine Human Herpesviruses

Infections with the nine human herpesviruses (HHVs) are globally prevalent and characterized by lifelong persistence. Reactivations can potentially manifest as life-threatening conditions for which the demonstration of viral DNA is essential. In the present study, we developed HERQ-9, a pan-HHV quantitative PCR designed in triplex reactions to differentiate and quantify each of the HHV-DNAs: (i) herpes simplex viruses 1 and 2 and varicella-zoster virus; (ii) Epstein-Barr virus, human cytomegalovirus, and Kaposi's sarcoma-associated herpesvirus; and (iii) HHV-6A, -6B, and -7. The method was validated with prequantified reference standards as well as with mucocutaneous swabs and cerebrospinal fluid, plasma, and tonsillar tissue samples. Our findings highlight the value of multiplexing in the diagnosis of many unsuspected, yet clinically relevant, herpesviruses. In addition, we report here frequent HHV-DNA co-occurrences in clinical samples, including some previously unknown. HERQ-9 exhibited high specificity and sensitivity (LOD95 of similar to 10 to similar to 17 copies/reaction), with a dynamic range of 10' to 10 6 copies/p.I. Moreover, it performed accurately in the coamplification of both high- and low-abundance targets in the same reaction. In conclusion, we demonstrated that HERQ-9 is suitable for the diagnosis of a plethora of herpesvirus-related diseases. Besides its significance to clinical management, the method is valuable for the assessment of hitherto-unexplored synergistic effects of herpesvirus coinfections. Furthermore, its high sensitivity enables studies on the human virome, often dealing with minute quantities of persisting HHVs. IMPORTANCE By adulthood, almost all humans become infected by at least one herpesvirus (HHV). The maladies inflicted by these microbes extend beyond the initial infection, as they remain inside our cells for life and can reactivate, causing severe diseases. The diagnosis of active infection by these ubiquitous pathogens includes the detection of DNA with sensitive and specific assays. We developed the first quantitative PCR assay (HERQ-9) designed to identify and quantify each of the nine human herpesviruses. The simultaneous detection of HHVs in the same sample is important since they may act together to induce life-threatening conditions. Moreover, the high sensitivity of our method is of extreme value for assessment of the effects of these viruses persisting in our body and their long-term consequences on our health.Peer reviewe

Software para el cálculo de la huella ambiental en la producción de cacao

El presente artículo contiene aspectos importantes en las diferentes etapas del cultivo de cacao, además, los componentes representativos, agentes involucrados e indicadores para el cálculo de la huella de carbono e hídrica, que mejora el sistema de producción de la comunidad de cacaocultores del Huila con el desarrollo de un Software el cual, se aborda con una metodología ágil a través del método SCRUM. En la etapa de análisis se toma las variables involucradas en la producción de cacao, y las normativas ambientales internacionales vigente

Third San Juan photoelectric astrolabe catalogue (CPASJ3)

Resulting from the cooperation between Beijing, San Juan and La Plata Astronomical Observatories, the photoelectric astrolabe Mark II(PAII) of the Beijing Astronomical Observatory was moved and installed at the San Juan Observatory, Argentina in January, 1992 for observations of stars in the southern hemisphere. Using the data observed with the instrument from Feb. 23 1992 to Mar. 11, 2000 over 2382 days, the Third San Juan photoelectric astrolabe catalogue has been compiled from double transits at both the eastern and western passages. There are 6762 stars in this catalogue, including 6156 Hipparcos stars (in which there are 69 radio stars), 8 FK5 stars, 47 SRS stars, 551 CAMC4 stars. The mean precisions are ±3.0 ms and ±0.053'' in right ascension and declination, respectively. The magnitudes of stars are from 1.0 to 11.5. The declinations are from -3° to -60°. The mean epoch is 1996.3. Systematic corrections of (CPASJ3-Hipp) are given.Facultad de Ciencias Astronómicas y Geofísica

Classical Ising model test for quantum circuits

We exploit a recently constructed mapping between quantum circuits and graphs in order to prove that circuits corresponding to certain planar graphs can be efficiently simulated classically. The proof uses an expression for the Ising model partition function in terms of quadratically signed weight enumerators (QWGTs), which are polynomials that arise naturally in an expansion of quantum circuits in terms of rotations involving Pauli matrices. We combine this expression with a known efficient classical algorithm for the Ising partition function of any planar graph in the absence of an external magnetic field, and the Robertson-Seymour theorem from graph theory. We give as an example a set of quantum circuits with a small number of non-nearest neighbor gates which admit an efficient classical simulation.Comment: 17 pages, 2 figures. v2: main result strengthened by removing oracular settin

Combined genetic approaches yield a 48% diagnostic rate in a large cohort of French hearing-impaired patients

International audienceHearing loss is the most common sensory disorder and because of its high genetic heterogeneity, implementation of Massively Parallel Sequencing (MPS) in diagnostic laboratories is greatly improving the possibilities of offering optimal care to patients. We present the results of a two-year period of molecular diagnosis that included 207 French families referred for non-syndromic hearing loss. Our multi-step strategy involved (i) DFNB1 locus analysis, (ii) MPS of 74 genes, and (iii) additional approaches including Copy Number Variations, in silico analyses, minigene studies coupled when appropriate with complete gene sequencing, and a specific assay for STRC. This comprehensive screening yielded an overall diagnostic rate of 48%, equally distributed between DFNB1 (24%) and the other genes (24%). Pathogenic genotypes were identified in 19 different genes, with a high prevalence of GJB2, STRC, MYO15A, OTOF, TMC1, MYO7A and USH2A. Involvement of an Usher gene was reported in 16% of the genotyped cohort. Four de novo variants were identified. This study highlights the need to develop several molecular approaches for efficient molecular diagnosis of hearing loss, as this is crucial for genetic counselling, audiological rehabilitation and the detection of syndromic forms