Computer-assisted radiographic calculation of spinal curvature in brachycephalic "screw-Tailed" dog breeds with congenital thoracic vertebral malformations: reliability and clinical evaluation

The objectives of this study were: To investigate computer-assisted digital radiographic measurement of Cobb angles in dogs with congenital thoracic vertebral malformations, to determine its intra- and inter-observer reliability and its association with the presence of neurological deficits. Medical records were reviewed (2009–2013) to identify brachycephalic screw-tailed dog breeds with radiographic studies of the thoracic vertebral column and with at least one vertebral malformation present. Twenty-eight dogs were included in the study. The end vertebrae were defined as the cranial end plate of the vertebra cranial to the malformed vertebra and the caudal end plate of the vertebra caudal to the malformed vertebra. Three observers performed the measurements twice. Intraclass correlation coefficients were used to calculate the intra- and inter-observer reliabilities. The intraclass correlation coefficient was excellent for all intra- and inter-observer measurements using this method. There was a significant difference in the kyphotic Cobb angle between dogs with and without associated neurological deficits. The majority of dogs with neurological deficits had a kyphotic Cobb angle higher than 35°. No significant difference in the scoliotic Cobb angle was observed. We concluded that the computer assisted digital radiographic measurement of the Cobb angle for kyphosis and scoliosis is a valid, reproducible and reliable method to quantify the degree of spinal curvature in brachycephalic screw-tailed dog breeds with congenital thoracic vertebral malformations

Development of learning objectives for neurology in a veterinary curriculum: part I: undergraduates

Background With an increasing caseload of veterinary neurology patients in first opinion practice, there is a requirement to establish relevant learning objectives for veterinary neurology encompassing knowledge, skills and attitudes for veterinary undergraduate students in Europe. With help of experts in veterinary neurology from the European College of Veterinary Neurology (ECVN) and the European Society of Veterinary Neurology (ESVN) a survey of veterinary neurologic learning objectives using a modified Delphi method was conducted. The first phase comprised the development of a draft job description and learning objectives by a working group established by the ECVN. In the second phase, a quantitative questionnaire (multiple choice, Likert scale and free text) covering 140 learning objectives and subdivided into 8 categories was sent to 341 ESVN and ECVN members and a return rate of 62% (n = 213/341) was achieved. Results Of these 140 learning objectives ECVN Diplomates and ESVN members considered 42 (30%) objectives as not necessary for standard clinical veterinary neurology training, 94 (67%) were graded to be learned at a beginner level and 4 (3%) at an advanced level. The following objectives were interpreted as the most important day one skills: interpret laboratory tests, perform a neurological examination and establish a neuroanatomical localization. In this survey the three most important diseases of the central nervous system included epilepsy, intervertebral disc disease and inflammatory diseases. The three most important diseases of the peripheral nervous system included polyradiculoneuritis, myasthenia gravis and toxic neuropathies. Conclusions The results of this study should help to reform the veterinary curriculum regarding neurology and may reduce the phenomenon of “Neurophobia”.</p

State–society relations in the ‘South African developmental state’: integrated development planning and public participation at the local level

Philosophiae Doctor - PhDIn various formulations, the idea of a developmental state has appeared in official discourse in South Africa since the advent of democratic government in 1994, albeit that its adoption as state policy has been slow, uneven and inconsistent with the original East Asian model. What has been a feature of developmental state thinking in South Africa is the fact that the concept has been so poorly articulated in policy that it has come to mean different things to different state actors and to the public. This has been aggravated by the fact that the idea of a strongly interventionist developmental state has run counter to the idea of a diminished state enunciated in various neo-liberal policies. Moreover, unlike the authoritarian and top-down East Asian model, the government envisages a South African developmental state which is infused with democratic content where citizens assist in the formulation of policy from below. In its emphasis on a bottom-up approach to policy formulation the South African model differs markedly from the conventional idea of a developmental state which is heavily reliant on a strong central bureaucracy to drive economic growth. In the South African model local government has been assigned a pivotal role in addressing persistent economic exclusion and uneven development. A central tenet of this approach is the need for local authorities to institutionalise participatory processes at grassroots level and devise effective structures and processes to facilitate citizen participation in local affairs. In the light of the above, this thesis sets out to examine the manner in which a system of developmental local government is being implemented in the City of Cape Town. Taking as a case study the township of Delft, the study looks at the systems and processes (and particularly the process of integrated development planning) set in place to advance citizen participation. It examines the extent to which the model is perceived to be achieving its goals from the perspective of political office bearers, officials from different spheres of government and residents. The research found that notwithstanding an enabling legislative and policy framework, there is little comprehension of, or interest, in the idea of developmental local government and municipal officials largely pay lip service to participatory processes which are carried out in a top-down fashion and which neither empower local residents nor enhance their welfare. It also concluded that developmental government, in its present form, is contributing little to the establishment of a national developmental state

Feline Spongiform Encephalopathy: A Study of the Clinical Presentation and Pathology

Feline Spongiform Encephalopathy (FSE) was first reported in 1990 by Wyatt et al., over three years after Bovine Spongiform Encephalopathy (BSE) was first identified. At present only limited published clinical and pathological descriptions of FSE are available. The aims of this thesis are therefore to review the current literature on FSE and describe the clinical and pathological features of the cases in this study. The nine naturally occurring cases of FSE included in this thesis were presented to Glasgow University Veterinary School during the period of June 1990 to April 1998. A set of clinical criteria was identified allowing a presumptive ante-mortal diagnosis of FSE. These included: progressive behavioural changes, hyperaesthesia, ataxia, hypersalivation and intermittent pupillary dilatation, although not all where present in every' case. No evidence of a breed or sex predilection, geographic clustering, reliable routine diagnostic test or successful treatment modality could be identified. The nature and distribution of the pathological changes are very similar in all cases in this series, with the significant changes confined to the central nervous system (CNS). The changes include vacuolation of grey matter neuropil, vacuolation of individual neurones, Wallerian-type degeneration and a reactive astrocytosis. Neuropil vacuolation is widespread, but certain regions (including the cerebellar granule cell layer, deep cerebral layers, thalamus, basal nuclei and septal nuclei) appear to be preferentially affected. Vacuolation of individual neurones is selectively present in the dorsal nucleus of the vagal nerve and raphe nuclei, and occasionally present in the hypoglossal nucleus, vestibular nuclei and reticular formation. Characteristic patterns of pathological PrP accumulation in the CNS are evident on PrP immunocytochemistry, using monoclonal antibody 3F4 raised against hamster PrP. Aspects of the spongiform changes and PrP immunocytochemistry in these cases bear strong similarities to new variant CJD (vCJD), although with some differences. These similarities are consistent with circumstantial evidence suggesting that both FSE and vCJD originated from ingestion of material contaminated with the infective agent responsible for BSE (Bruce et al. 1997; Collinge et al. 1996)

Leading change in the South African District Health Service

Purpose - The paper sets out to explore the leadership processes and dynamics of change management in a fragmented, and resource-poor, health service in an impoverished rural region in South Africa. Design/methodology/approach - The paper outlines an action research process aimed at assisting the stakeholders of two rural clinics to integrate psychiatric care into the Primary Health Care service that they offer their respective communities. This involved the transformation of existing practices through a form of praxis that involved learning from action and acting on learning. Findings - The findings of the paper relate to the role of leadership in the facilitation of transformational learning in team-based social action. Four areas of leadership responsibility are highlighted: the transformation of inappropriate mental models; the development of strategic resilience; the shifting of the locus of control of stakeholders to a more internal position; and the creation of a social environment in which intangible capital resources are generated and leveraged in the collective interest. Research limitations/implications - This paper is subject to the limitations of potential bias and distortion in action research. Although the "objective" evidence of the integration of psychiatric services at Pelsrus and Kwanomzamo clinics exists, the portrayal of the learning processes through which this was achieved could have been influenced unwittingly by the authors' own knowledge and other interests. Practical implications - The paper endorses the educational importance of work-based projects through which strong tacit leadership knowledge bases can be developed in health sector personnel

Incomplete ossification of the atlas in a dog: surgical stabilisation using a SOP plate

No abstract available

Browse : quantity and nutritive value of evergreen and deciduous tree species in semi-arid Southern African savannas.

Thesis (Ph.D.)-University of KwaZulu-Natal, Pietermaritzburg, 2012.Browse selection, intake, utilisation, palatability, quality and production are tightly linked and need to be considered together in trying to improve our understanding of browsing dynamics and the interactions between browsers and vegetation. Such an understanding is necessary in order to re-evaluate determinations of browser carrying capacities and evaluating actual and potential impacts of browsing animals on vegetation composition and diversity. Browser carrying capacity is determined by both the quantity and the nutritive value of forage. The measurement of browse quantity and nutritive value and the matching of browse supply to browser demand are central to sustainable utilisation and the monitoring of vegetation health. South African savannas are poorly studied with respect to tree canopy growth and browse production making it difficult to quantify the available browse biomass on which browsing capacity estimations are based, and consequently difficult to estimate levels of browsing that are sustainable. This study addressed these issues by investigating browse dynamics, broadly aiming to (1) explore factors affecting browse production, biomass and nutritive value; (2) develop models to assess and monitor these parameters across seasons and properties; (3) use the resultant models in improving our understanding of how to determine browser carrying capacities. More specifically, our study sought to examine the effects of plant physiognomy, forage nutritive value, canopy stratum, defoliation, temperature, rainfall and soil nutrient status on the browse production of evergreen (Carissa bispinosa, Euclea divinorum, Gymnosporia senegalensis), semi-deciduous (Spirostachys africana, Ziziphus mucronata) and deciduous (Acacia nilotica, Dichrostachys cinerea) savanna tree species from June 2003 – June 2005 in three sites along the northern Zululand coastline of KwaZulu-Natal. Available browse biomass, during the dry season, of four key savanna tree species (A. nilotica, E. divinorum, G. senegalensis, and S. africana) was estimated through the development of allometric regression equations. Non-linear regression was used to investigate the relation between the leaf dry mass (LDM) and canopy volume (CVol) of each of the four tree species. Exponential regression (y = a + brlnx) of the natural logarithm of CVol data provided the most accurate and precise description of the tree CVol – LDM relation. A study was undertaken to determine which factors may influence browse production in a southern African savanna. Regression tree models for the browse production identified that the dominant factors influencing browse production were CVol (m3), season, species and height to the lowest leaves of the tree canopy (HL) (m). The length of the growing season had a marked effect on the production potential of savanna tree species, suggesting that improved conditions for growth, i.e. greater rainfall, soil moisture content and improved soil nutrient availability result in a longer period of rapid sustained growth. Species was identified as an important contributing factor to differences in browse production rates, suggesting the need for the development of species or species group models. Mean annual browse production of evergreen trees was greater than that of deciduous and semi-deciduous trees. Mean quarterly (three monthly) browse production was highest, for all trees, during the wet season, with the greatest difference between wet and dry season production being observed in deciduous forms. Evergreen forms showed continuous growth over the whole study, with enhanced growth over the wet season. Deciduous forms, on the other hand, concentrated growth in spurts, when environmental conditions became favourable, with most production occurring during a short growing season. Browse nutritive value was found to be greatest during the wet season, when growth and photosynthesis are at their greatest. Further, browse nutritive value was greatest in deciduous species. Evergreen trees were found to have greater acid detergent fibre (ADF) concentrations than both the deciduous and semi-deciduous trees. By contrast, crude protein (CP) concentrations were greater in semi-deciduous and deciduous species than in evergreen species. The daily CP requirements for maintenance for an adult impala (45 kg) were met by all species over all three study areas and all seasons. Daily CP requirements for growth and lactation, however, were only ever met by deciduous and semi-deciduous species, though this result was not consistent over study areas and seasons. Predictive models for the production of browse on deciduous, semi-deciduous and evergreen trees in northern Zululand were developed using multivariate adaptive regression spline functions. The best predictors of growing season browse production in all three tree guilds (defined here as a group of trees having a characteristic mode of living) were primarily measurable tree dimensions, while the prevailing environmental conditions had little impact. Differences in the production, nutritive value and available browse biomass between the different tree forms and seasons have a profound effect on the determination of browser carrying capacities and need to be incorporated into any game or conservation management plan

Idiopathic head tremor in english bulldogs

Idiopathic head tremor (IHT) syndrome is a recognized but poorly characterized movement disorder in English bulldogs (EBs). The data analyzed were collected via a detailed online questionnaire and video recordings. Thirty-eight percent of the population demonstrated IHT. The first presentation was early in life. There was no sex or neutered status predisposition. The condition disappeared with time in 50% of the cases. The direction of the head movement was vertical or horizontal. The number of episodes per day and the duration of the episodes were greatly variable. The majority of episodes occurred at rest. Most of the episodes were unpredictable. And there was no alteration of the mental status for most dogs during the episodes. Stress has been reported as a suspected trigger factor. IHT in EBs can be considered an idiopathic paroxysmal movement disorder

The Chihuahua dog: A new animal model for neuronal ceroid lipofuscinosis CLN7 disease?

Neuronal ceroid lipofuscinoses (NCLs) are a group of incurable lysosomal storage disorders characterized by neurodegeneration and accumulation of lipopigments mainly within the neurons. We studied two littermate Chihuahua dogs presenting with progressive signs of blindness, ataxia, pacing, and cognitive impairment from 1 year of age. Because of worsening of clinical signs, both dogs were euthanized at about 2 years of age. Postmortem examination revealed marked accumulation of autofluorescent intracellular inclusions within the brain, characteristic of NCL. Whole-genome sequencing was performed on one of the affected dogs. After sequence alignment and variant calling against the canine reference genome, variants were identified in the coding region or splicing regions of four previously known NCL genes (CLN6, ARSG, CLN2 [=TPP1], and CLN7 [=MFSD8]). Subsequent segregation analysis within the family (two affected dogs, both parents, and three relatives) identified MFSD8:p.Phe282Leufs13*, which had previously been identified in one Chinese crested dog with no available ancestries, as the causal mutation. Because of the similarities of the clinical signs and histopathological changes with the human form of the disease, we propose that the Chihuahua dog could be a good animal model of CLN7 disease

Parallel Mapping and Simultaneous Sequencing Reveals Deletions in BCAN and FAM83H Associated with Discrete Inherited Disorders in a Domestic Dog Breed

The domestic dog (Canis familiaris) segregates more naturally-occurring diseases and phenotypic variation than any other species and has become established as an unparalled model with which to study the genetics of inherited traits. We used a genome-wide association study (GWAS) and targeted resequencing of DNA from just five dogs to simultaneously map and identify mutations for two distinct inherited disorders that both affect a single breed, the Cavalier King Charles Spaniel. We investigated episodic falling (EF), a paroxysmal exertion-induced dyskinesia, alongside the phenotypically distinct condition congenital keratoconjunctivitis sicca and ichthyosiform dermatosis (CKCSID), commonly known as dry eye curly coat syndrome. EF is characterised by episodes of exercise-induced muscular hypertonicity and abnormal posturing, usually occurring after exercise or periods of excitement. CKCSID is a congenital disorder that manifests as a rough coat present at birth, with keratoconjunctivitis sicca apparent on eyelid opening at 10–14 days, followed by hyperkeratinisation of footpads and distortion of nails that develops over the next few months. We undertook a GWAS with 31 EF cases, 23 CKCSID cases, and a common set of 38 controls and identified statistically associated signals for EF and CKCSID on chromosome 7 (Praw 1.9×10−14; Pgenome = 1.0×10−5) and chromosome 13 (Praw 1.2×10−17; Pgenome = 1.0×10−5), respectively. We resequenced both the EF and CKCSID disease-associated regions in just five dogs and identified a 15,724 bp deletion spanning three exons of BCAN associated with EF and a single base-pair exonic deletion in FAM83H associated with CKCSID. Neither BCAN or FAM83H have been associated with equivalent disease phenotypes in any other species, thus demonstrating the ability to use the domestic dog to study the genetic basis of more than one disease simultaneously in a single breed and to identify multiple novel candidate genes in parallel