Proteomic analysis of flowers at two developmental stages in Thermopsis turcica (Fabaceae)

Flower development is a complex physiological phenomenon that is affected by environmental stimuli and endogenous signals. Several molecular approaches have provided a comprehensive view of the physiological processes associated with flower development, but little is known about proteomic changes. Therefore, we investigated the proteomic alterations during flower development in endemic Thermopsis turcica (Vuralia turcica), an unusual legume species with 3–4 free carpels in a single flower. A comparative proteomic analysis to identify proteins involved in flower development was carried out in T. turcica. Alterations in proteomes of the flower buds and fully opened flowers were studied by two-dimensional gel electrophoresis. A total of 66 proteins were differentially expressed during flower development. Among them, 32 protein spots were identified by mass spectrometry. Functional annotation of these flower proteins revealed their involvement in transcription and protein metabolism, energy and carbohydrate metabolisms, plant defense, cell walls, photosynthesis, secondary metabolism, and amino acid metabolism. Moreover, most of these proteins were downregulated at the later stage of flower development. Proteins involved in protein metabolism, sugar metabolism, and stress defense are regulated during flower development, suggesting that they have possible roles in developmental regulation. These results have given new insights into the proteome alterations during flower development

Rectal duplications accompanying rectovestibular fistula: Report of two cases

Rectal duplication (RD) cysts are rare congenital anomalies that can be diagnosed with the presence of another opening in the perineum. They seldom accompany anorectal malformations (ARM). Two cases of RD accompanying ARM at opposite ends of the phenotypic spectrum, are described. A 3-month-old baby and a 2-year-old girl with ARM were scheduled for posterior sagittal anorectoplasty. The infant had an orifice at the anal dimple and the other had an orifice at the vestibulum posterior to the rectovestibular fistula. The infant presented with no other anomalies whereas the older one presented with an unusual coexistence of caudal duplication and caudal regression syndromes. Perioperatively both orifices were found to be related to retrorectal cysts, and were excised. Clinicians should always be alert when dealing with complex malformations. Because these malformations have variable anatomical and clinical presentations, they can represent a diagnostic and therapeutic challenge

Sol-Jel yöntemiyle borlanmış inconel alaşımının yüzey karakterizasyonu

Bu çalışmada, Inconel alaşımı sol-jel mettodu kullanılarak borlanmıştır. Borlama sonrası numuneler elektrik rezistanslı fırında 900○C'de 1 saat bekeltilerek sinterlemiştir. Numuneler kesitten kesilerek gerekli metolografik işlemlerden geçilirek numunelerin borür tabaka kalınlıkları Nikon MA100 marka optik mikroskop yardımıyla ölçülmüştür. Borlanmış numunelede oluşan fazların analizi Shimadzu XRD-6000 model X-ışınları cihazı yardımıyla tespit edilmiştir. Borlama işlemi sonucunda Inconel 625 alaşımında NiB, Ni2B, Ni3B, Ni4B3, MoB, CrB ve Cr2B fazları elde edilmiştir. Borlama sıcaklık ve süresine bağlı olarak 4.1-9.7 μm arasında borür tabakaları elde edilmiştir. Üç farklı bor bileşiğinde farklı mikro sertlik değerleri elde edilmiştir. Tinkal’de 1673 HV0.1, Sassolit’de 1997 HV0.1, B4C’de 2375 HV0.1, İşlemsiz Inconel 625 alaşımınında ise 541 HV0.1 sertlik değerleri elde edilmiştir.In this study, surface characterization of boronized inconel alloy was investigated by sol gel method. The samples were sintered at 900ºC for 1 hour in a furnace with electrical resistance. The boride samples were cut from the section and the boride layer thicknesses obtained after the required sanding and polishing processes were measured with the help of Nikon MA100 optic microscope. Borate phases were obtained by using Shimadzu XRD 6000 brand XRD device and micro hardness tests were carried out with Shimadzu HMV-2 brand tester. As a result of XRD analysis of boron Inconel samples, NiB, Ni2B, Ni3B, Ni4B3, MoB, CrB and Cr2B phases were obtained. Depending on boron temperature and time, boron layers were obtained on Inconel samples with thicknesses ranging from 4.1-9.7 μm. The micro hardness values of the boronized Inconel samples were 541 HV0.1 in the untreated sample while the hardness values of tinkal 1673 HV0.1, Sassolit 1997 HV0.1 and boron carbide 2375 HV0.1 were obtained

Impact of opioid-free analgesia on pain severity and patient satisfaction after discharge from surgery: multispecialty, prospective cohort study in 25 countries

Background: Balancing opioid stewardship and the need for adequate analgesia following discharge after surgery is challenging. This study aimed to compare the outcomes for patients discharged with opioid versus opioid-free analgesia after common surgical procedures.Methods: This international, multicentre, prospective cohort study collected data from patients undergoing common acute and elective general surgical, urological, gynaecological, and orthopaedic procedures. The primary outcomes were patient-reported time in severe pain measured on a numerical analogue scale from 0 to 100% and patient-reported satisfaction with pain relief during the first week following discharge. Data were collected by in-hospital chart review and patient telephone interview 1 week after discharge.Results: The study recruited 4273 patients from 144 centres in 25 countries; 1311 patients (30.7%) were prescribed opioid analgesia at discharge. Patients reported being in severe pain for 10 (i.q.r. 1-30)% of the first week after discharge and rated satisfaction with analgesia as 90 (i.q.r. 80-100) of 100. After adjustment for confounders, opioid analgesia on discharge was independently associated with increased pain severity (risk ratio 1.52, 95% c.i. 1.31 to 1.76; P < 0.001) and re-presentation to healthcare providers owing to side-effects of medication (OR 2.38, 95% c.i. 1.36 to 4.17; P = 0.004), but not with satisfaction with analgesia (beta coefficient 0.92, 95% c.i. -1.52 to 3.36; P = 0.468) compared with opioid-free analgesia. Although opioid prescribing varied greatly between high-income and low- and middle-income countries, patient-reported outcomes did not.Conclusion: Opioid analgesia prescription on surgical discharge is associated with a higher risk of re-presentation owing to side-effects of medication and increased patient-reported pain, but not with changes in patient-reported satisfaction. Opioid-free discharge analgesia should be adopted routinely

De-centring the securitization of asylum and migration in the European Union: Securitization, vulnerability and the role of Turkey

This article contributes to the debates on de-centring the analysis of migration governance in Europe by focusing on the potential role of external actors in the securitisation of asylum and migration in the European Union (EU). Although there has been a growing amount of literature on the securitisation of asylum and migration in the EU, the role possibly played by external actors in this securitisation process has not been considered to date. This article addresses this gap using the case of Turkey. Theoretically, it contributes to the development of the securitisation framework by de-centring the study of securitisation processes. It argues that, from the vantage point of an external actor, a securitisation process highlights the existence of a vulnerability to a specific phenomenon that is perceived to be threatening. An external actor can then decide to exploit this vulnerability for its own gain, notably by making threats that play on the fears of the other political actor. Empirically, the article demonstrates how the Turkish government has been able to exploit the vulnerability of European countries to migration flows, which had been highlighted by the social construction of asylum and migration as security issues. By repeatedly threatening to send more asylum-seekers and migrants Europe’s way, the Turkish authorities have managed to secure some significant financial and political benefits for themselves in the last few years

Sıçanlarda doksorubisin ile oluşturulmuş kardiyotoksisite üzerine melatonin ve timokinonun etkileri

Bu çalışmanın amacı doksorubisinle oluşturulan kalp hasarına karşı timokinonun olası kalbi koruyucu etkisini araştırmak ve bunu melatoninin nispeten daha iyi etkisiyle karşılaştırmaktı. Deneylerde 8 haftalık, ağırlıkları 230-350 gr olan 32 adet Wistar albino erkek sıçan kullanıldı. Denekler dört gruba ayrıldı: Kontrol, Doksorubisin (DXR), Doksorubisin+Melatonin (DXR+MEL) ve Doksorubisin+Timokinon (DXR+TQ). Yedi gün boyunca DXR+MEL grubuna intraperitoneal olarak her gün 10 mg/kg melatonin, DXR+TQ grubuna pelet yeme eklenmek suretiyle her gün 50 mg/kg timokinona verildi. Deneyin 5. gününde tüm tedavi gruplarına kuyruk veninden tek doz DXR (45 mg/kg) kontrol grubuna ise serum fizyolojik enjekte edildi. Deneyin 8. gününde anestezi altında EKG kayıtları alındı. Hayvanlar dekapite edildikten sonra biyokimyasal ve histolojik incelemeler için kalp dokuları çıkarıldı. Elektrokardiyografik, biyokimyasal ve histolojik farklılıkları karşılaştırmak için verilerin Kruskal Wallis, Mann Whitney U ve Fisher Freeman Halton Exact (sadece histolojik bulguların istatistiksel analizinde) testleri kullanıldı. Timokinon; DXR’ın uzattığı PR, QRS ve OTc sürelerini kısalttı oysaki melatonin sadece QTc süresini kısalttı. DXR’ın kalp dokusunda yol açtığı histopatolojik değişiklikler üzerinde melatoninin koruyucu etkisi vardı fakat timokinonun etkisi olmadı. DXR kalp dokusunda CK-MB, IL-1, IL-6, IL-18, MDA ve NO düzeylerini artırırken SOD’u azalttı. Melatonin CK-MB, IL-1, IL-6, IL-18, MDA, NO ve SOD düzeylerini düşürdü. Timokinon ise sadece CK-MB, IL-1 ve IL- 18’de azalmaya neden oldu. Sıçanlarda doksorubisinin kalpte neden olduğu hasar üzerinde melatoninin koruyucu bir etkisi vardır. Bu etki timokinonda zayıftır. Timokinonun olası dozla ilişkili etkileri ileri araştırmaları gerektirir. DXR kardiyotoksisitesi üzerinde her iki kimyasalın birlikte etkilerinin araştırılmasının da bu konuda faydalı olacağını düşünüyoruz

Investigation of neopteri̇n levels effectiveness i̇n diagnosis of the childhood appendi̇ci̇ti̇s

Akut apandisit çocukluk çağında en sık görülen acil cerrahi patolojidir. Negatif apendektomi ve perforasyon oranlarını azaltmak için yardımcı tanı yöntemleri geliştirilmektedir. Bu çalışmanın amacı aktive olmuş makrofajlardan salınan neopterinin (NPT) apandisitte tanısal değerliliğinin olup olmadığı araştırmaktır. Çalışmaya acil servis birime 12 saati geçen sağ alt kadran ağrısı ile başvuran akut apandisit ön tanısı ile çocuk cerrahisi servisine yatırılıp takip edilen 25'i kız toplam 76 olgu dâhil edildi. Servise yatışı esnasında beyaz küre (BK) ve C-reaktif protein (CRP) için serum örnekleri ve NPT ölçümü için idrar örnekleri alındı. Takipleri sonucunda karın ağrısı gerileyen grubun (Grup K) CRP, BK ve NPT değerleri ile akut apandisit grubu (Grup A) nun değerleri arasında farklılık olduğu, ancak bu farklılığın CRP ve NPT değerleri için istatistiksel olarak anlamlı olduğu (p0,05). Perfore apandisit (Grup P) grubu ve Grup A'nın BK, CRP ve NPT seviyeleri arasındaki farklılık değerlendirildiğinde, istatistiksel olarak aradaki farkın anlamlı olduğu gösterildi (p<0,001). İnflamatuar belirteçlerin duyarlılık ve özgüllükleri ise CRP'nin %82-%82, BK'nın %76-%52, NPT'nin ise %78-%61 olarak hesaplandı. Sonuç olarak akut ve perfore apandisit tanısında BK ve CRP etkili iken perforasyonun ayırıcı tanısında CRP'nin etkinliği daha yüksektir. Neopterin hem karın ağrılı olguların apandisitten ayırıcı tanısında hem de akut ve perfore olguların ayırıcı tanısında kullanılabilecek bir inflamatuar belirteçtir.Acute appendicitis, which still has high negative laparotomy and perforation ratios, is the most common surgical pathology in childhood with. In order to decrease these negative ratios many studies are conducting in order to develop a more effective diagnostic test. In this study it was aimed to evaluate the diagnostical value of the neopterin in the diagnosis of appendicitis. Seventy-seven patients referring to the emergency department with abdominal pain were included in this study. Their blood samples were obtained for WBC and C- reactive protein studies and urine samples were collected for neopterin analysis. The results of the Group K and Group A found to be different. These differences for CRP and neopterin found to be statistically significant, whereas the difference for WBC found to be insignificant. The result in Group P is found to be the highest for WBC, CRP and neopterin. And the difference between the Group P and the other groups found to be significant. The sensitivity and specificity of inflammatory markers were found to be 82%-82% CRP, 76%-52% WBC and 78%-61% neopterin. In conclusion, neopterin seems to be a promising agent in differential diagnosis of the appendicitis

Nightmare of lumbar disc surgery: iliac artery injury

WOS: 000235227000011PubMed: 16412838Intra-operative injuries of pelvic vessels are quite infrequent but serious complications of lumbar disc surgery. Injury may cause laceration of the vessel with acute life threatening retropentoneal haemorrhage, formation of pseudoaneurysms or arteriovenous fistulae in the late phase. It is important for surgeons to be aware of these potentially fatal complications. Authors reported a case of major vascular injury recognised intra-operatively during posterior lumbar discectomy and repaired immediately. (c) 2004 Elsevier B.V. All rights reserved

Alt ekstremite lenfödem hastalarında kinezyofobi, fiziksel performans ve denge arasındaki ilişki

Purpose: Lymphedema is an important situation that causes physical and psychological lifethreatening problems. This study researches kinesiophobia in lower extremity patients and scrutinizes the relationship between kinesiophobia, physical performance and balance. Methods: This cross-sectional controlled study included 40 individuals diagnosed with lower extremity lymphedema with a mean age of 42.58±10.30 years and 31 healthy individuals with a mean age of 40.65 ± 9.53 years. The evaluation of patients with unilateral lymphedema without cognitive and visual problems and those without orthopedic and neurological disorders that would prevent walking and those without heart disease and hypertension was carried out between November 2018 and March 2019. Static balance was evaluated with standing on One leg Balance Test, fear of movement was evaluated with Tampa Scale Kinesiophobia and functional levels were evaluated with Timed Up and Go test. Results. Static balance and physical performance of lower extremity lymphedema patients were different from healthy individuals (p<0.05). Static balance and physical performance were lower in these patients than in healthy individuals. Patients with lower extremity lymphedema had a severe fear of movement (37 and above). There was a significant moderate correlation between kinesiophobia and balance (r= -0.536 p= 0.001), kinesiophobia and physical performance (r= 0.522 p= 0.001) in lower extremity lymphedema patients. And this correlation was more in healthy individuals. Conclusions: High fear of movement and decreased physical performance and balance were found in patients with lower extremity lymphedema. Early diagnosis and treatment of complications due to lymphedema is important for the clinical results of this patient group.Amaç: Lenfödem, fiziksel ve psikolojik yaşamı tehdit eden sorunlara neden olan önemli bir durumdur. Bu çalışma, alt ekstremite hastalarında kinezyofobiyi araştırmakta ve kinezyofobi, fiziksel performans ve denge arasındaki ilişkiyi incelemektedir. Yöntem: Bu kesitsel kontrollü çalışmaya, yaş ortalaması 42,58±10,30 yıl olan alt ekstremite lenfödem tanısı konan 40 kişi ve yaş ortalaması 40,65 ± 9,53 yıl olan 31 sağlıklı birey dahil edildi. Tek taraflı lenfödemi bulunan ve bilişsel ve görsel sorunu olmayan, yürümeyi engelleyecek ortopedik ve nörolojik bozukluğu olmayan, kalp hastalığı ve hipertansiyonu olmayan hastaların değerlendirilmesi Kasım 2018-Mart 2019 tarihleri arasında yapıldı. Statik denge tek ayak üzerinde durma testi ile hareket korkusu Tampa Kinezyofobi Ölçeği ile, fonksiyonel düzeyleri ise Zamanlı Kalk Yürü Testi ile değerlendirildi. Sonuçlar: Alt ekstremite lenfödem hastalarının statik dengesi ve fiziksel performansı sağlıklı bireylerden farklıydı (p<0,05). Bu hastalarda statik denge ve fiziksel performans sağlıklı bireylere göre daha düşüktü. Alt ekstremite lenfödemli hastalarda şiddetli hareket korkusu vardı (37 ve üzeri). Alt ekstremite lenfödem hastalarında kinezyofobi ile denge (r= -0,536 p= 0,001), kinezyofobi ve fiziksel performans (r= 0,522 p= 0,001) arasında orta düzeyde anlamlı bir ilişki vardı. Bu korelasyon sağlıklı bireylerde daha fazlaydı. Tartışma: Alt ekstremite lenfödemli hastalarda yüksek hareket korkusu, fiziksel performans ve dengede azalma bulundu. Lenfödeme bağlı komplikasyonların erken teşhisi ve tedavisi bu hasta grubunun klinik sonuçları açısından önemlidir.No sponso

A Novel Control and Monitoring Interface Design for ROS Based Mobile Robots

In this study, an interface design was carried out in order to provide convenience to the user in the control and monitoring of the Robot Operating System (ROS) based autonomous mobile robot (AMR). Qt Designer and Python were used in the interface design. Thanks to the designed interface, autonomous and manual control of AMR was provided. Using the gmapping algorithm, the environment in the virtual world was mapped and transformed into a picture in .png format and visualized in the interface. The location information from the ROS was transferred to the said picture and the instant tracking of the AMR was done via the interface. It was shown which algorithm is used locally and globally at that moment. While in autonomous mode, the vehicle was provided to move to the previously recorded point. The total distance and time spent by the AMR while moving between two points were also calculated by the interface. The location (x, y, z) and orientations (x, y, z, w) of the previously recorded station were monitored from the stop list. On the other hand, the position (x, y, z) and orientation (x, y, z, w) information of the AMR could be followed as real time via the interface. In this way, when the AMR reaches the goal station, the time elapsed between two points, the transportation distance, settlement information such as the location and orientation of the vehicle can be tracked and be compared via the interface