The Investigation of Antidiabetic Effects of Leontice leontopetalum Extract on Human Pancreatic β Cell Lines (1.1B4) Treated with Streptozotocin

One of the alternative therapeutic methods is herbal medicine. Leontice leontopetalum belongs to Berberidaceae family. The aim of study was investigated the extract of LL on human pancreatic beta cell-treated with STZ. Materials and methods: The human pancreatic beta cell (1.1B4) line was used the current study. LL’s extracts (1, 10, 100, and 1000 ug/ml) were supplemented in media for twenty-four hours and/or after STZ treatment (10 and 20 mM). Cells survivals (MTT), cells proliferation were shown by using xCelligence. Insulin content and releasing were measured at 1.1, 8.4 and 16.7 mM glucose concentrations. Results: The result of MTT was shown that cell survival was decreased, based on dose-dependent. When looked at xCelligence results, cell proliferation in STZ groups and the lowest and highest concentrations of LL were attenuated in a dose-dependent manner. Also, cotreatments of LL and STZ were decreased as well. The result of insulin-releasing on glucose induction was shown that STZ concentration gave rise to reduce insulin content at low and high glucose levels. Also, co-treatment of LL and STZ attenuated insulin content based on dose. Conclusion: It was considered that LL treatment led to increased insulin realizing, resulting from decreasing insulin content in diabetic beta cells, but decrease cell survival

The prevalence of nutritional anemia in pregnancy in an east Anatolian province, Turkey

<p>Abstract</p> <p>Background</p> <p>Anemia is considered a severe public health problem by World Health Organization when anemia prevalence is equal to or greater than 40% in the population. The purpose of this study was to determine the anemia prevalence with the associated factors in pregnant women and to determine the serum iron, folate and B12 vitamin status in anaemic pregnants in Malatya province.</p> <p>Methods</p> <p>This is a cross-sectional survey. A multi-sage stratified probability-proportional-to-size cluster sampling methodology was used. A total of 823 pregnant women from sixty clusters were studied. Women were administered a questionnaire related with the subject and blood samples were drawn. Total blood count was performed within four hours and serum iron, folate and B12 vitamin were studied after storing sera at -20 C for six months.</p> <p>Results</p> <p>Anemia prevalence was 27.1% (Hb < 11.0 gr/dl). Having four or more living children (OR = 2.2), being at the third trimester (OR = 2.3) and having a low family income (OR = 1.6) were determined as the independent predictors of anemia in pregnancy. Anemia was also associated with soil eating (PICA) in the univariate analysis (p < 0.05). Of anaemic women, 50.0% had a transferrin saturation less than 10% indicating iron deficiency, 34.5% were deficient in B12 vitamin and 71.7% were deficient in folate. Most of the anemias were normocytic-normochromic (56.5%) indicating mixed anemia.</p> <p>Conclusions</p> <p>In Malatya, for pregnant women anemia was a moderate public health problem. Coexisting of iron, folate and B vitamin deficiencies was observed among anaemics. To continue anemia control strategies with reasonable care and diligence was recommended.</p

Robust estimation of bacterial cell count from optical density

Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals &lt;1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data

Nouveaux systèmes réducteurs utilisant des hydrosiloxanes comme substituts des hydrures de bore et d’aluminium : Application à la réduction de fonctions azotées et oxygénées

In recent years, industrial and academic researches have experienced unprecedented changes related tothe concept of sustainable development. The choice of raw material, reactions, processes, andcharacterization of final products are constraining the industrials to rethink their production methods.This change can be accomplished by “the conception of chemical products and processes that reduceor eliminate the use and synthesis of hazardous materials.” The research work described in this thesisis focused on the development of new reducing systems using hydrosiloxanes as substitutes foraluminum and boron hydrides.In this context, several reducing systems based on the use of 1,1,3,3-tetramethyldisiloxane (TMDS)associated with a metal complex have been developed in the laboratory. Depending on the metal,selective reductions of various organic functions were performed. Thus, iron (III) acetylacetonate andTMDS allowed the reduction of aromatic nitro compounds; palladium on charcoal was used for thesynthesis of alcoxysiloxanes from oxygenated rings, alcohols or carbonyl compounds; esters were alsoreduced into alcohols by the action of a molybdenum or a vanadium complex; reduction of carboxylicacids as well as the cylization of dicarboxylic acids have been carried out using indium tribromide andthe latter complex also proved efficiency for the reduction of phosphine oxides derivativesCes dernières années, les recherches industielles et académiques ont connu des bouleversements sansprécédents liés à la notion de Développement Durable. Le choix des matières premières, des réactions,des procédés, et la caractérisation du produit final obligent les industriels à repenser leur mode deproduction. Ce changement peut notamment s’effectuer par « la conception de produits et de procédéschimiques permettant de réduire ou d’éliminer l’utilisation et la synthèse de leurs substancesdangereuses. » Les travaux de recherche décrits dans cette thèse s’inscrivent dans ce contexte etconcernent la mise au point de nouveaux systèmes réducteurs utilisant des hydrosiloxanes commesubstituts des hydrures d’aluminium et de bore.Dans cet objectif, plusieurs systèmes réducteurs basés sur l’utilisation du 1,1,3,3-tétraméthyldisiloxane(TMDS) associé à un complexe métallique ont pu être développés au laboratoire. Selon la nature dumétal employé, les réductions sélectives de diverses fonctions organiques ont été effectuées. Ainsi, lefer (III) acétylacétonate associé au TMDS a permis la réduction de nitro aromatiques en anilinescorrespondantes ; le palladium sur charbon la synthèse d’alcoxysiloxanes à partir de cycles oxygénés,d’alcools ou de carbonyles ; les esters ont également été réduits en alcools par action d’un complexede molybdène ou de vanadium ; la réduction d’acides ainsi que la cyclisation de diacides ont pu êtreeffectuées à l’aide de tribromure d’indium et ce dernier complexe a également montré une bonneefficacité pour la réduction d’oxydes de phosphine

Nouveaux systèmes réducteurs utilisant des hydrosiloxanes comme substituts des hydrures de bore et d’aluminium : Application à la réduction de fonctions azotées et oxygénées

In recent years, industrial and academic researches have experienced unprecedented changes related tothe concept of sustainable development. The choice of raw material, reactions, processes, andcharacterization of final products are constraining the industrials to rethink their production methods.This change can be accomplished by “the conception of chemical products and processes that reduceor eliminate the use and synthesis of hazardous materials.” The research work described in this thesisis focused on the development of new reducing systems using hydrosiloxanes as substitutes foraluminum and boron hydrides.In this context, several reducing systems based on the use of 1,1,3,3-tetramethyldisiloxane (TMDS)associated with a metal complex have been developed in the laboratory. Depending on the metal,selective reductions of various organic functions were performed. Thus, iron (III) acetylacetonate andTMDS allowed the reduction of aromatic nitro compounds; palladium on charcoal was used for thesynthesis of alcoxysiloxanes from oxygenated rings, alcohols or carbonyl compounds; esters were alsoreduced into alcohols by the action of a molybdenum or a vanadium complex; reduction of carboxylicacids as well as the cylization of dicarboxylic acids have been carried out using indium tribromide andthe latter complex also proved efficiency for the reduction of phosphine oxides derivativesCes dernières années, les recherches industielles et académiques ont connu des bouleversements sansprécédents liés à la notion de Développement Durable. Le choix des matières premières, des réactions,des procédés, et la caractérisation du produit final obligent les industriels à repenser leur mode deproduction. Ce changement peut notamment s’effectuer par « la conception de produits et de procédéschimiques permettant de réduire ou d’éliminer l’utilisation et la synthèse de leurs substancesdangereuses. » Les travaux de recherche décrits dans cette thèse s’inscrivent dans ce contexte etconcernent la mise au point de nouveaux systèmes réducteurs utilisant des hydrosiloxanes commesubstituts des hydrures d’aluminium et de bore.Dans cet objectif, plusieurs systèmes réducteurs basés sur l’utilisation du 1,1,3,3-tétraméthyldisiloxane(TMDS) associé à un complexe métallique ont pu être développés au laboratoire. Selon la nature dumétal employé, les réductions sélectives de diverses fonctions organiques ont été effectuées. Ainsi, lefer (III) acétylacétonate associé au TMDS a permis la réduction de nitro aromatiques en anilinescorrespondantes ; le palladium sur charbon la synthèse d’alcoxysiloxanes à partir de cycles oxygénés,d’alcools ou de carbonyles ; les esters ont également été réduits en alcools par action d’un complexede molybdène ou de vanadium ; la réduction d’acides ainsi que la cyclisation de diacides ont pu êtreeffectuées à l’aide de tribromure d’indium et ce dernier complexe a également montré une bonneefficacité pour la réduction d’oxydes de phosphine

New reducing systems using hydrosiloxanes as substitutes of boron and aluminum hydrides : Application to the reduction of nitrogenous and oxygenated organic functions

Ces dernières années, les recherches industielles et académiques ont connu des bouleversements sansprécédents liés à la notion de Développement Durable. Le choix des matières premières, des réactions,des procédés, et la caractérisation du produit final obligent les industriels à repenser leur mode deproduction. Ce changement peut notamment s’effectuer par « la conception de produits et de procédéschimiques permettant de réduire ou d’éliminer l’utilisation et la synthèse de leurs substancesdangereuses. » Les travaux de recherche décrits dans cette thèse s’inscrivent dans ce contexte etconcernent la mise au point de nouveaux systèmes réducteurs utilisant des hydrosiloxanes commesubstituts des hydrures d’aluminium et de bore.Dans cet objectif, plusieurs systèmes réducteurs basés sur l’utilisation du 1,1,3,3-tétraméthyldisiloxane(TMDS) associé à un complexe métallique ont pu être développés au laboratoire. Selon la nature dumétal employé, les réductions sélectives de diverses fonctions organiques ont été effectuées. Ainsi, lefer (III) acétylacétonate associé au TMDS a permis la réduction de nitro aromatiques en anilinescorrespondantes ; le palladium sur charbon la synthèse d’alcoxysiloxanes à partir de cycles oxygénés,d’alcools ou de carbonyles ; les esters ont également été réduits en alcools par action d’un complexede molybdène ou de vanadium ; la réduction d’acides ainsi que la cyclisation de diacides ont pu êtreeffectuées à l’aide de tribromure d’indium et ce dernier complexe a également montré une bonneefficacité pour la réduction d’oxydes de phosphine.In recent years, industrial and academic researches have experienced unprecedented changes related tothe concept of sustainable development. The choice of raw material, reactions, processes, andcharacterization of final products are constraining the industrials to rethink their production methods.This change can be accomplished by “the conception of chemical products and processes that reduceor eliminate the use and synthesis of hazardous materials.” The research work described in this thesisis focused on the development of new reducing systems using hydrosiloxanes as substitutes foraluminum and boron hydrides.In this context, several reducing systems based on the use of 1,1,3,3-tetramethyldisiloxane (TMDS)associated with a metal complex have been developed in the laboratory. Depending on the metal,selective reductions of various organic functions were performed. Thus, iron (III) acetylacetonate andTMDS allowed the reduction of aromatic nitro compounds; palladium on charcoal was used for thesynthesis of alcoxysiloxanes from oxygenated rings, alcohols or carbonyl compounds; esters were alsoreduced into alcohols by the action of a molybdenum or a vanadium complex; reduction of carboxylicacids as well as the cylization of dicarboxylic acids have been carried out using indium tribromide andthe latter complex also proved efficiency for the reduction of phosphine oxides derivative

Effect Of Hıgh Fat Dıet Or Dıet Enrıched Wıth Monounsaturated Fatty Acıds On Dıabetıc Rats On Glycemıc Control And Lıpıd Profıle

Bu çalışma diyabetik wistar albino ratlarına uygulanan iki farklı test diyeti olan yüksek ve tekli doymamış yağ asitleriyle zenginleştirilmiş çok yüksek yağlı diyet ile kontrol diyetlerinin glisemik kontrol ve lipid profili üzerine etkilerini incelemek üzere yürütülmüştür. Trakya Üniversitesi Deney Hayvanları Birimi nde yürütülen bu çalışmaya 36 adet wistar albino erkek rat dahil edilmiştir. Bir grubu kontrol grubu (KG) olarak belirlenmiş ve diğer 3 gruba streptozotosin (35 mg/kg) ile diyabet oluşturulmuştur. Araştırmanın hazırlık aşaması, başlangıç ve sonunda ratlardan kan numuneleri alınmış ve vücut ağırlığı takibi yapılmıştır. Dört haftalık deney sonunda tekli doymamış yağ asitleriyle zenginleştirilmiş çok yüksek yağlı diyabet (ÇYYDG) grubunun diğer gruplara göre açlık plazma glukoz (APG) düzeyi ve vücut ağırlığında (VA) önemli derecede azalma saptanmıştır (p0,05), ÇYYDG nda azalma düzeyinin en yüksek olduğu dikkat çekicidir (p0,05). Çok yüksek yağlı diyabet grubunda APG ve VA (r=0,787, p=0,012) arasında güçlü, pozitif yönde anlamlı ilişki bulunmuştur. Yüksek yağlı diyet ile beslenen diyabetik ratlarda APG ve TG düzeyleri (r=0,889, p=0,019), APG ve VLDL-K (r=0,889, p=0,019) düzeyleri arasında pozitif yönde ve anlamlı çok güçlü bir ilişki bulunmuştur. Sonuç olarak, diyabetin tıbbi beslenme tedavisinde diyette tekli doymamış yağ asitlerinin ve posa düzeyinin arttırılması glisemik kontrol ve lipid profilinin iyileştirilmesinde olumlu etkiye sahiptir; ancak etkilerinin değerlendirilmesi için daha büyük örneklemli ve geniş kapsamlı çalışmalara ihtiyaç duyulmaktadır.This study was conducted to investigate the effects two different test diets (high fat and enriched with monounsaturated fatty acids high fat diet) on glycemic control and lipid profile on diabetic wistar albino rats. Thirty-six Wistar albino male rats were included in the study conducted at the Trakya University Experimental Animal Unit. Streptozotocin (35 mg / kg) used for diabetes in 3 groups and one was a control group. The blood samples were taken of rats at baseline and at the end were taken and followed body weight. At the end of the experiment (4 weeks), fasting plasma glucose (FPG) level and body weight (BW) of enriched with monounsaturated fatty acids high fat diet diabetic group (MUFAHFDG) were significantly decreased compared to the other groups (p 0,05), but the decrease level was the highest in MUFAHFDG (p 0,05). There was a significant positive correlation between FPG and BW (r=0,787, p=0,012) in MUFAHFDG. There was a significant positive correlation between FPG and TG (r=0,889, p=0,019), FPG and VLDL-C (r=0,889, p=0,019) in high fat fed diabetic rats group. As a result, diet enriched with monounsaturated fatty acids and increased fibre may be usefull to medical nutrition therapy on improving glycemic control and lipid profile in diabetes, but there is a need for larger sample and extensive studies for further evaluation of its effects

Assesment Of Nutrutıon Literacy And Diet Selffeffıcacy Belıefs In Adult Induvıduals Receıvıng Medical Nutrutıon Theraphy: Example of Isparta

Bu araştırma, Isparta Şehir Hastanesi Diyet Polikliniği’ne herhangi bir kronik hastalık nedeniyle tıbbi beslenme tedavisi almak için başvuran gönüllü bireylerin katılımıyla yürütülmüştür. Çalışmada tıbbi beslenme tedavisi alması gereken bireylerin beslenme okuryazarlığı ve diyet özetkinlik inançlarının değerlendirilmesi amaçlanmıştır. Tüm bireylere demografik özellikler, antropometrik ölçümler, yetişkinler için beslenme okuryazarlığı değerlendirme aracı ve diyet özetkinlik inançları ölçeğini içeren anket formu yüz yüze uygulanmıştır. Kadın ve erkek katılımcıların diyet özetkinlik inançları ölçeği toplam puanı sırasıyla 120,23±25,72 ve 118,58±20,87’dir. Diyet özetkinlik inançları ölçeği toplam puanı ile sadece kadın katılımcıların; yaş, eğitim durumu, BKİ, bel ve kalça çevreleri ile anlamlı bir ilişki saptanmıştır (p<0,05). Çalışmaya dâhil edilen bireylerin beslenme okuryazarlığı her iki cinsiyet için sınır düzeyindedir. Çalışmaya katılan kadın bireylerin beslenme okuryazarlık düzeyi arttıkça; yaş, vücut ağırlığı, olmak istediği vücut ağırlığı, BKİ ve kalça çevre düzeyleri azalmakta olup (p0,05). Kadın katılımcıların beslenme okuryazarlık puanı ile yaş, eğitim durumu, yaşadığı yer, vücut ağırlığı, olmak istediği vücut ağırlığı, BKİ, bel ve kalça çevreleri arasındaki ilişki anlamlı olup (p<0,05), erkek katılımcılarda; yaş, eğitim durumu ve yaşadığı yer arasında anlamlı bir ilişki bulunmuştur (p<0,05). Çalışmaya katılan bireylerin diyet özetkinlik inançları ölçeği ve yetişkinlerde beslenme okuryazarlığı değerlendirme aracının diğer toplam puan ve alt bölümleri arasında pozitif yönde bir ilişki saptanmıştır (p<0,05). Sonuç olarak bireylerin beslenme okuryazarlık düzeyleri yükseldikçe diyet özetkinlik inançları da yükselmekte ve böylece tıbbi beslenme tedavisini sürdürebileceklerine dair inançları da artmaktadır.This research was carried out with the participation of volunteer individuals who applied to Isparta City Hospital Diet Polyclinic for medical nutrition treatment due to any chronic disease. In this study, it was aimed to evaluate the nutritional literacy and diet selfefficacy beliefs of individuals who should receive medical nutrition therapy. A questionnaire including demographic characteristics, anthropometric measurements, nutritional literacy assessment tool for adults and diet self-efficacy beliefs scale was applied face to face. The total score of diet self-efficacy beliefs scale of female and male participants is 120,23±25,72 and 118,58±20,87 respectively. A significant correlation was found with the total score of diet self-efficacy beliefs scale only with the age, education level, BMI, waist and hip circumference of female participants (p<0,05). Nutrition literacy of the individuals included in the study is at the limit level for both sexes. As the nutritional literacy level of female individuals participating in the study increases; age, body weight, body weight she want to be, BMI and hip circumference levels decrease (p<0,05), but this effect was not observed in men (p>0,05). The relationship between the nutritional literacy score of women participants and their age, education, place of residence, body weight, body weight they want to be, BMI, waist and hip circumference is significant (p<0,05). A significant relation was found between age, education level and place of residence in male participants (p<0,05). The relationship between female participants nutritional literacy score and age, educational status, place of 73 residence, body weight, body weight they want to be, BMI, waist and hip circumference is significant (p<0.05). A significant relation was found between age, education level and place of residence in male participants (p<0.05). A positive correlation was found between the diet self-efficacy beliefs scale of the individuals participating in the study and other total scores and subsections of the nutritional literacy assessment tool in adults. As a result; as the nutritional literacy levels of individuals increase, their diet self-efficacy beliefs also increase, thus increasing their belief that they can continue medical nutrition therapy

Determination of Nutrition Knowledge Levels of Teachers Working in Edirne Center

Objective: This study was carried out to determine the nutritional knowledge levels of teachers working in Edirne Center. Methods: A total of 389 teachers, 272 female (69.9%) and 117 male (30.1%) volunteers working at schools affiliated to the Republic of Turkish Ministry of National Education in Edirne Center and agreeing to participate in the study. The research data were collected by face-to-face interview method using a questionnaire form. The data of the research were evaluated using the SPSS 19.0 package program. Findings: The average age of teachers participating in the study was 42.67 +/- 8.76 years. Teachers' nutritional knowledge score average was 4.61 +/- 1.36. There was no significant relationship between teachers' age, gender, marital status, education, branch of study and body mass index (BMI) and their nutritional knowledge scores (p> 0.05). There was no statistically significant relationship between teachers' access to nutritional information source, food label reading knowledge, emphasizing healthy nutrition in their lessons giving nutritional advice to their students and nutritional behavior affects students' behavior (p> 0.05). There was a negative relationship between age and nutritional knowledge score, and it was significant at 0.09 level ( r = -0.093. p = 0.067). The relationship between the nutritional knowledge score and body mass index (BMI) was not found to be significant (p> 0.05). Result: In the study, the nutritional knowledge levels of teachers did not reach the expected goals for each teacher. The implementation of nutrition education programs and the frequent evaluation of the results of teachers, who shape the future and are role models for students, will lead future generations to gain healthy eating habits.WOS:00070496930002