Revisiting the iron pools in cucumber roots: identification and localization

Iron may accumulate in various chemical forms during its uptake and assimilation in roots. The permanent and transient Fe microenvironments formed during these processes in cucumber which takes up Fe in a reduction based process (Strategy I), have been investigated. The identification of Fe microenvironments was carried out with 57Fe Mössbauer spectroscopy and immunoblotting, whereas reductive washing and high resolution microscopy was applied for the localization. In plants supplied with 57FeIII-citrate, a transient presence of Fe-carboxylates in removable forms and the accumulation of partly removable, amorphous hydrous ferric oxide/hydroxyde have been identified in the apoplast and on the root surface, respectively. The latter may at least partly be the consequence of bacterial activity at the root surface. Ferritin accumulation did not occur at optimal Fe supply. Under Fe deficiency, highly soluble ferrous hexaaqua complex is transiently formed along with the accumulation of Fe-carboxylates, likely Fe-citrate. As 57Fe-citrate is non-removable from the root samples of Fe deficient plants the major site of accumulation is suggested to be the root xylem. Reductive washing results in another ferrous microenvironment remaining in the root apoplast, the FeII-bipyridyl complex, which accounts for ~30% of the total Fe content of the root samples treated for 10 min and rinsed with CaSO4 solution. When 57FeIII-EDTA or 57FeIII-EDDHA was applied as Fe-source higher soluble ferrous Fe accumulation was accompanied by a lower total Fe content, confirming that chelates are more efficient in maintaining soluble Fe in the medium while less stable natural complexes as Fe-citrate may perform better in Fe accumulation

Change in Magnetic Anisotropy at the Surface and in the Bulk of FINEMET Induced by Swift Heavy Ion Irradiation

57 Fe transmission and conversion electron Mössbauer spectroscopy as well as XRD were used to study the effect of swift heavy ion irradiation on stress-annealed FINEMET samples with a composition of Fe73.5 Si13.5 Nb3 B9 Cu1. The XRD of the samples indicated changes neither in the crystal structure nor in the texture of irradiated ribbons as compared to those of non-irradiated ones. However, changes in the magnetic anisotropy both in the bulk as well as at the surface of the FINEMET alloy ribbons irradiated by 160 MeV132 Xe ions with a fluence of 1013 ion cm−2 were revealed via the decrease in relative areas of the second and fifth lines of the magnetic sextets in the corresponding Mössbauer spectra. The irradiation-induced change in the magnetic anisotropy in the bulk was found to be similar or somewhat higher than that at the surface. The results are discussed in terms of the defects produced by irradiation and corresponding changes in the orientation of spins depending on the direction of the stress generated around these defects. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.CZ-11/2007, MEB040806; Ministry of Education and Science of the Russian Federation, Minobrnauka: FEUZ-2020-0060; Hungarian Scientific Research Fund, OTKA: K100424, K115784, K115913, K43687, K68135; Joint Institute for Nuclear Research, JINR; Univerzita Palackého v Olomouci: CZ.02.1.01/0.0/0.0/17_049/0008408, IGA_PrF_2022_003, IGA_PrF_2022_013; Ural Federal University, UrFU: 04-5-1131-2017/2021; Nemzeti Kutatási Fejlesztési és Innovációs Hivatal, NKFIHFunding: The research was supported by grants from the Hungarian National Research, Development and Innovation Office (OTKA projects No K43687, K68135, K100424, K115913, K115784) and by the Czech-Hungarian Intergovernmental Fund, Grant No. CZ-11/2007 (MEB040806). M.I.O. was supported by the Ministry of Science and Higher Education of the Russian Federation, project No. FEUZ-2020-0060. Additionally, M.I.O. was supported in part by the Ural Federal University project within the Priority-2030 Program, funded from the Ministry of Science and Higher Education of the Russian Federation. This work was also supported by the project “Swift heavy ions in research of iron-bearing nanomaterials”, No. of theme 04-5-1131-2017/2021, solved in cooperation with the Czech Republic and the JINR (3 + 3 projects), and also by internal IGA grant of Palacký University (IGA_PrF_2022_003). The authors from Palacký University Olomouc want to thank the facilitators of project CZ.02.1.01/0.0/0.0/17_049/0008408 of the Ministry of Education, Youth & Sports of the Czech Republic for their support as well.Acknowledgments: We are grateful to Z. Klencsár (Centre for Energy Research, Budapest), M. Miglierini (Technical University, Bratislava), I. Dézsi (Wigner Research Centre for Physics, Budapest), S. Kubuki, and K. Nomura (Tokyo Metropolitan University, Tokyo) for their participation in discussions, and L. Krupa (Czech Technical University in Prague, Czech Republic and Joint Institute for Nuclear Research, Dubna) for his help with the organization of project cooperation. The support by grants from the Hungarian National Research, Development and Innovation Office and by the Czech-Hungarian Intergovernmental Fund, Grant No. CZ-11/2007 (MEB040806) are acknowledged. M.I.O. is grateful for support from the Ministry of Science and Higher Education of the Russian Federation and from the Ural Federal University project within the Priority-2030 Program. This work was also carried out within the Agreement of Cooperation between the Ural Federal University (Ekaterinburg) and the Eötvös Loránd University (Budapest) and within the Memorandum of Understanding between the Ural Federal University (Ekaterinburg) and the Palacký University (Olomouc). Authors acknowledge the support of the project “Swift heavy ions in research of iron-bearing nanomaterials”, No. of theme 04-5-1131-2017/2021, solved in cooperation with the Czech Republic and the JINR (3 + 3 projects). Authors from Palacký University Olomouc appreciate the internal IGA grant of Palacký University (IGA_PrF_2022_013) and thank the facilitators of the project CZ.02.1.01/0.0/0.0/17_049/0008408 of the Ministry of Education, Youth & Sports of the Czech Republic as well

Drilling wells by rotary hammer technology

Import 26/06/2013Tato práce je zaměřena na zřizování a konstrukční provedení vrtané studny pro jímání vody vhodnou technologií. Seznamujeme s potřebnými legislativními postupy potřebnými k samotnému hloubení studny. Dále navrhuje provedení vlastní konstrukce vrtu a zvolení vhodné vrtné technologie.This work is focused on the creation and design of the drilled wells for water abstraction appropriate technology. Getting familiar with the necessary legislative procedures necessary to the actual digging wells. Furthermore, we propose to design our own design and choosing suitable borehole drilling technology.Prezenční542 - Institut hornického inženýrství a bezpečnostivýborn

Imagine supported multimedial education

The goal of this bachelor work is to create a methodical manual for development environment Imagine. This manual deals with the use of its multimedia objects and tries to explain basic conceptions and technical informations, that relate to multimedia. For this porpuse is for better understanding and illustation made a set of projects implemented in Imagine

Design of drilled wells

Import 22/07/2015Tato diplomová práce je zaměřena na provedení konstrukce vrtané studny a možnosti technologického provedení v konkrétních geologických podmínkách. Sestavením posloupnosti úkonů před započetím a v průběhu vrtných prací se zaměřuji na provedení hydrogeologického vrtu s následným využitím jako zdroj vody pro celoroční provoz rodinného domu. Před samotnou realizací hydrogeologického vrtu HV-1, jsem se zabýval provedením konstrukce v geologických podmínkách a odvozením optimální volby technologického řešení vrtu se zřetelem na ochranu podzemních vod.This thesis focuses on the design and construction of drilled walls. Also different forms of technological design in specific geological conditions are discussed. Described is the sequence of activities before starting and during drilling work of hydrogeological well. Drill will subsequently be used as water source for year-round operation of the house. Certain conditions has to be considered prior HV-1 hydrogeological drilling. For example drill construction in actual geological conditions and optimal choose of technological solution. Also groundwater protections is key aspect of drilling works discussed in this thesis.Prezenční542 - Institut hornického inženýrství a bezpečnostivýborn

New Ferrocene-Based Metalloligand with Two Triazole Carboxamide Pendant Arms and Its Iron(II) Complex: Synthesis, Crystal Structure, ⁵⁷Fe Mössbauer Spectroscopy, Magnetic Properties and Theoretical Calculations

The new ferrocene-based metalloligand bis (N-4-[3,5-di-(2-pyridyl)-1,2,4-triazoyl])ferrocene carboxamide (L) was prepared through derivatization of 1,1′-ferrocenedicarboxylic acid with 4-amino-3,5-di(pyridyl)-4H-1,2,4-triazole. The composition and purity of L in the solid state was determined with elemental analysis, FT-IR spectroscopy, and its crystal structure with single-crystal X-ray analysis, which revealed that the substituted cyclopentadienyl rings adopt the antiperiplanar conformation and the crystal structure of L is stabilized by O–H···N and N–H···O hydrogen bonds. The molecular properties of L in solution were investigated with NMR and UV-VIS spectroscopies, and cyclic voltammetry disclosed irreversible redox behavior providing one oxidation peak at E1/2 = 1.133 V vs. SHE. Furthermore, the polymeric FeII complex {Fe(L)(C(CN)3)2}n (1) was prepared and characterized with elemental analysis, FT-IR spectroscopy, 57Fe Mössbauer spectroscopy, and magnetic measurements. The last two methods confirmed that a mixture of low- and high-spin species is present in 1; however, the spin crossover properties were absent. The presented study was also supported by theoretical calculations at the DFT/TD-DFT level of theory using TPSS and TPSSh functionals

Successive Grinding and Polishing Effect on the Retained Austenite in the Surface of 42CrMo4 Steel

Low-alloy 42CrMo4 steels were studied by Fe-57 Mossbauer spectroscopy (MS), X-ray diffractometry (XRD), and Energy Dispersive X-ray Spectroscopy (EDS) measurements. The investigations were performed on metallographic samples, which were subjected to a series of successive grinding and polishing with a progressively finer grit. Conversion X-ray Mossbauer spectroscopy (CXMS) was used to determine the occurrence of austenite in steel samples. It is a unique method detecting the austenite content very sensitively. Six samples with different surface preparation were investigated, starting with 4.8% of austenite on an as-cut sample, and a large decrease in the retained austenite to 2.6% was observed after the first grinding of a hardened cut sample. Additionally, an unexpectedly large decrease in the austenite content to 2.3% was found due to the final polishing. A second time applied successive grinding and polishing of all samples resulted in identical austenite content determined by CXMS of approx. 5%, which proved the applicability of the CXMS method. Generally, the result calls attention to the importance of preparation of metallurgical samples by grinding and polishing where the results can vary significantly on the level of surface processing