Involuntary evaluation of others' emotional expressions depends on the expresser's group membership. Further evidence for the social message account from the extrinsic affective Simon task

The social message account (SMA) hypothesizes that the evaluation of emotional facial expressions depends on the ethnicity of the expressers. For example, according to SMA, a happy face of a member of a prejudiced ethnicity is immediately interpreted as potentially malevolent. Evidence for this approach was found initially in evaluative priming (EP) and approach-avoidance tasks (AA) by showing an emotion × ethnicity interaction on positivity scores (EP) and approach scores (AA), respectively. Recently, attempts to replicate the EP results failed. Due to the inconclusive EP results, it was important to examine the influence of ethnicity on processing of emotional expression with another task testing involuntary evaluations. The extrinsic affective Simon task was used with stimuli varying on emotion (happy vs. fear) and ethnicity (White-Caucasian vs. Middle-Eastern men). This task was chosen because in contrast to EP (where faces are presented as task-irrelevant primes) faces are task-relevant. Experiment 1 yielded an emotion × ethnicity interaction with regard to positivity scores that fit SMA predictions. The results are also important in challenging a recent theoretical alternative to SMA, namely the processing conflict account. A generalization of the emotion × ethnicity pattern to learned arbitrary in- and out-groups (Experiment 2) failed, suggesting that involuntary processing of (task-irrelevant) group status depends on perceptual features

Evaluating Aftereffects of Short-Duration Transcranial Random Noise Stimulation on Cortical Excitability

A 10-minute application of highfrequency (100–640 Hz) transcranial random noise stimulation (tRNS) over the primary motor cortex (M1) increases baseline levels of cortical excitability, lasting around 1 hr poststimulation Terney et al. (2008). We have extended previous work demonstrating this effect by decreasing the stimulation duration to 4, 5, and 6 minutes to assess whether a shorter duration of tRNS can also induce a change in cortical excitability. Single-pulse monophasic transcranial magnetic stimulation (TMS) was used to measure baseline levels of cortical excitability before and after tRNS. A 5- and 6-minute tRNS application induced a significant facilitation. 4-minute tRNS produced no significant aftereffects on corticospinal excitability. Plastic after effects after tRNS on corticospinal excitability require a minimal stimulation duration of 5 minutes. However, the duration of the aftereffect of 5-min tRNS is very short compared to previous studies using tRNS. Developing different transcranial stimulation techniques may be fundamental in understanding how excitatory and inhibitory networks in the human brain can be modulated and how each technique can be optimised for a controlled and effective application

Threatening joy : examining the process underlying concordant and divergent reactions to in-group and out-group emotions

Zahlreiche Studien zeigen, dass Menschen kongruente affektive Reaktionen auf die emotionalen Gesichtsausdrücke von Anderen zeigen: Positive Ausdrücke lösen positive affektive Reaktionen aus, und negative Ausdrücke negative Reaktionen. Es wurde argumentiert, dass diese Reaktionen – zumindest zum Teil – durch die Intention ausgelöst werden, welche durch einen emotionalen Ausdruck signalisiert wird. Diese Argumentation legt nahe, dass eine spezifische Emotion eine spezifische Intention ausdrückt, was wiederum zu einer spezifischen Reaktion führt. Wir nehmen jedoch an, dass der Zusammenhang zwischen emotionalem Ausdruck, Intention, und affektiver Reaktion viel flexibler ist: In Abhängigkeit von sozialen und kontextuellen Faktoren kann der gleiche emotionale Ausdruck unterschiedliche Intentionen ausdrücken und dadurch unterschiedliche Reaktionen auslösen. Solch ein sozialer Faktor, der die Interpretation eines emotionalen Gesichtsausdrucks beeinflusst, könnte die Gruppenzugehörigkeit der Person sein, welche die Emotion zeigt. Da Gruppenzugehörigkeit die Beziehung zwischen Menschen beeinflusst, beeinflusst sie auch das Licht, in dem die Emotion gesehen wird. Wenn die Person, welche die Emotion zeigt, und die Person, welche sie wahrnimmt, der gleichen Gruppe angehören, ist die Beziehung zwischen ihnen freundliche und kooperativ; folglich wird auch die Intention in diesem Licht gesehen und eine kongruente Reaktion auslösen. Falls jedoch die Person, welche die Emotion zeigt, und die Person, welche sie wahrnimmt, unterschiedlichen Gruppen angehören, ist die Beziehung zwischen ihnen negative und kompetitiv. Die Intention wird entsprechend auch in diesem Licht gesehen und kann daher eine divergente Reaktion auslösen. Diese Annahme wurde in den Experimenten für diese Dissertation untersucht. Die Ergebnisse zeigen, dass Annäherungs- und Vermeidungsreaktionen auf Emotionen der Eigen- und Fremdgruppe tatsächlich von der Intention beeinflusst werden: Emotionen (Freude, Angst), die von Eigengruppenmitgliedern gezeigt wurden, lösten kongruente Annäherungs- und Vermeidungsreaktionen aus. Emotionale Ausdrücke (Freude, Angst) der Fremdgruppe aktivierten jedoch divergente Annäherungs- und Vermeidungsreaktionen. Da Annäherungs- und Vermeidungsreaktionen durch die Intention einer Emotion beeinflusst werden, unterstützen diese Ergebnisse unsere Annahme, dass die Gruppenzugehörigkeit beeinflusst, welche Intention aus einem emotionalen Ausdruck abgeleitet wird. Diese Schlussfolgerung wird zusätzlich durch Experiment unterstützt, welches auf der Reversed Correlation Technique basiert. Die Ergebnisse zeigen, dass die mentale Repräsentation von Lächeln gezeigt von Eigengruppenmitgliedern mit positiven Intentionen assoziiert ist, und das Lächeln von Fremdgruppenmitgliedern mit negativen Intentionen. Zusammengefasst zeigen die Ergebnisse, dass Gruppenzugehörigkeit automatische affektive Reaktionen auf Emotionen beeinflusst. Zusätzlich liefern sie Evidenz, dass dieser Effekt durch den Einfluss der Beziehung zwischen demjenigen, der die Emotion zeigt, und demjenigen, der sie wahrnimmt, auf die Interpretation der Intention verursacht wird.Numerous studies examining affective reactions to emotional expressions find that humans show concordant reactions to the emotions of others: Positive facial expressions activate positive affective reactions whereas negative facial expressions activate negative ones. It has been argued that these reactions are – at least in part – triggered by the intention signaled by the emotional expression. This arguing suggests that each emotion signals a specific intention leading to a specific reaction. However, we argue that the link between emotional expression, intention, and affective reaction is much more flexible: Depending on situational and social factors, the same emotional expression can be interpreted as signaling different intentions and therefore trigger different reactions. One of these social factors influencing the interpretation of an emotion might be the group membership of the expresser of the emotion. Since group membership influences the relationship between individuals, it influences the light in which the intention of the emotional expression is seen. If expresser and perceiver of an emotion share group membership, their relationship will be friendly and cooperative; accordingly, the intention signaled by the emotional expression will be seen in this light and elicit a concordant reaction. On the contrary, if expresser and perceiver are members of different groups, they will have a negative and competitive relationship. As a result, the intention signaled by this emotional expression should be interpreted in this light and might therefore elicit a divergent reaction. This assumption was examined in the experiments conducted for this thesis. The results show that approach and avoidance reactions to in-group and out-group emotions are indeed influenced by the intention signaled by the emotion: Emotions (happiness and fear) expressed by the in-group elicited concordant approach and avoidance reactions. Emotional expressions (happiness and fear) shown by out-group members, in contrast, elicited divergent approach and avoidance reactions. Since approach and avoidance reactions are influenced by the intention of the emotion, these results support our arguing that group membership influences which intention is inferred from an emotional expression. This conclusion is supported further by an experiment employing the reversed correlation technique. The results demonstrate that the mental representations of in-group smiles are associated with benevolent intentions and that of out-group smiles with malevolent ones. In sum, employing different paradigms, groups, and dependent variables, our results show that group membership influences automatic affective reactions to emotions. Furthermore, they provide evidence that this effect is caused by an influence of the relationship between expresser and perceiver of an emotion on the interpretation of the intention signaled by the emotional expression

Social Message Account or Processing Conflict Account – Which Processes Trigger Approach/Avoidance Reaction to Emotional Expressions of In- and Out-Group Members?

Faces are characterized by the simultaneous presence of several evaluation-relevant features, for example, emotional expression and (prejudiced) ethnicity. The social message account (SMA) hypothesizes the immediate integration of emotion and ethnicity. According to SMA, happy in-group faces should be interpreted as benevolent, whereas happy out-group faces should be interpreted as potentially malevolent. By contrast, fearful in-group faces should be interpreted as signaling an unsafe environment, whereas fearful out-group faces should be interpreted as signaling inferiority. In contrast, the processing conflict account (PCA) assumes that each face conveys two rather independent evaluative features, emotion and ethnicity. Thus, stimuli might be either affectively congruent or incongruent, and thereby exert influences on behavior. The article reviews the evidence with regard to the two accounts before reporting an experiment that aims at disentangling them. In an approach/avoidance task (AAT), either happy/fearful faces of German and Turks were presented or happy/fearful faces of young and old persons. There are prejudices against Turk/Middle-eastern persons (in Germany) as well as against old persons. For SMA, the two prejudices are of different type; thus prediction for the AAT diverge for the two group conditions. In contrast, for PCA both group features (i.e., Turk ethnicity and old age) are negative features (in comparison to their counterparts) which are affectively congruent or incongruent to the emotional expression. Hence, the results pattern in the AAT should be comparable for the two group conditions. In accordance with SMA but in contrast to PCA, we found different patterns for the two group conditions

Theta-gamma cross-frequency transcranial alternating current stimulation over the trough impairs cognitive control

Cognitive control is a mental process, which underlies adaptive goal-directed decisions. Previous studies have linked cognitive control to electrophysiological fluctuations in the theta band and theta-gamma cross-frequency coupling (CFC) arising from the cingulate and frontal cortices. Yet, to date the behavioral consequences of different forms of theta-gamma CFC remain elusive. Here, we studied the behavioral effects of the theta-gamma CFC via transcranial alternating current stimulation (tACS) designed to stimulate the frontal and cingulate cortices in humans. Using a double-blind, randomized, repeated measures study design, 24 healthy participants were subjected to three active and one control CFC-tACS conditions. In the active conditions, 80 Hz gamma tACS was coupled to 4 Hz theta tACS. Specifically, in two of the active conditions, short gamma bursts were coupled to the delivered theta cycle to coincide with either its peaks or troughs. In the third active condition, the phase of a theta cycle modulated the amplitude of the gamma oscillation. In the fourth, control protocol, 80 Hz tACS was continuously superimposed over the 4 Hz tACS, therefore lacking any phase-specificity in the CFC. During the 20-minute of stimulation, the participants performed a Go/NoGo monetary reward- and punishment-based instrumental learning task. A Bayesian hierarchical logistic regression analysis revealed that relative to the control, the peak-coupled tACS had no effects on the behavioral performance, whereas the trough-coupled tACS and, to a lesser extent, amplitude-modulated tACS reduced performance in conflicting trials. Our results suggest that cognitive control depends on the phase-specificity of the theta-gamma CFC

Influence of Concurrent Finger Movements on Transcranial Direct Current Stimulation (tDCS)-Induced Aftereffects

Transcranial direct current stimulation (tDCS) has been reported to have bidirectional influence on the amplitude of motor-evoked potentials (MEPs) in resting participants in a polarity-specific manner: anodal tDCS increased and cathodal tDCS decreased them. More recently, the effects of tDCS have been shown to depend on a number of additional factors. We investigated whether a small variety of movements involving target and non-target muscles could differentially modify the efficacy of tDCS. MEPs were elicited from the right first dorsal interosseous muscle, defined as the target muscle, by single pulse transcranial magnetic stimulation (TMS) over the primary motor cortex (M1). During M1 tDCS, which lasted for 10 min applying anodal, cathodal, or sham condition, the participants were instructed to squeeze a ball with their right hand (Task 1), to move their right index finger only in the medial (Task 2), in the lateral direction (Task 3), or in medial and lateral direction alternatively (Task 4). Anodal tDCS reduced MEP amplitudes measured in Task 1 and Task 2, but to a lesser extent in the latter. In Task 3, anodal tDCS led to greater MEP amplitudes than cathodal stimulation. Alternating movements resulted in no effect of tDCS on MEP amplitude (Task 4). The results are congruent with the current notion that the aftereffects of tDCS are highly variable relying on a number of factors including the type of movements executed during stimulation

Revisiting promyelocytic leukemia protein targeting by human cytomegalovirus immediate-early protein 1

This work was supported by a grant (MR/P022146/1) from the Medical Research Council (https://mrc.ukri.org) to MMN, a grant (T16/28) from Tenovus Scotland (https://tenovus-scotland.org.uk) to CP, a European Union Erasmus+ grant (https://www.erasmusplus.org.uk) to BW and the Wellcome Trust Institutional Strategic Support Fund (https://wellcome.ac.uk) to CP and MMN.Promyelocytic leukemia (PML) bodies are nuclear organelles implicated in intrinsic and innate antiviral defense. The eponymous PML proteins, central to the self-organization of PML bodies, and other restriction factors found in these organelles are common targets of viral antagonism. The 72-kDa immediate-early protein 1 (IE1) is the principal antagonist of PML bodies encoded by the human cytomegalovirus (hCMV). IE1 is believed to disrupt PML bodies by inhibiting PML SUMOylation, while PML was proposed to act as an E3 ligase for IE1 SUMOylation. PML targeting by IE1 is considered to be crucial for hCMV replication at low multiplicities of infection, in part via counteracting antiviral gene induction linked to the cellular interferon (IFN) response. However, current concepts of IE1-PML interaction are largely derived from mutant IE1 proteins known or predicted to be metabolically unstable and globally misfolded. We performed systematic clustered charge-to-alanine scanning mutagenesis and identified a stable IE1 mutant protein (IE1cc172-176) with wild-type characteristics except for neither interacting with PML proteins nor inhibiting PML SUMOylation. Consequently, IE1cc172-176 does not associate with PML bodies and is selectively impaired for disrupting these organelles. Surprisingly, functional analysis of IE1cc172-176 revealed that the protein is hypermodified by mixed SUMO chains and that IE1 SUMOylation depends on nucleosome rather than PML binding. Furthermore, a mutant hCMV expressing IE1cc172-176 was only slightly attenuated compared to an IE1-null virus even at low multiplicities of infection. Finally, hCMV-induced expression of cytokine and IFN-stimulated genes turned out to be reduced rather than increased in the presence of IE1cc172-176 relative to wild-type IE1. Our findings challenge present views on the relationship of IE1 with PML and the role of PML in hCMV replication. This study also provides initial evidence for the idea that disruption of PML bodies upon viral infection is linked to activation rather than inhibition of innate immunity.Publisher PDFPeer reviewe

IMPACT OF ANEMIA ON CLINICAL OUTCOME IN PATIENTS WITH ATRIAL FIBRILLATION UNDERGOING PERCUTANEOUS CORONARY INTERVENTION: INSIGHTS FROM THE AFCAS REGISTRY

OBJECTIVES: Anaemia has an adverse impact on the outcome in the general patient population undergoing percutaneous coronary intervention (PCI). The aim of this study was to analyse the impact of anaemia on the 12-month clinical outcome of patients with atrial fibrillation (AF) undergoing PCI and therefore requiring intense antithrombotic treatment. We hypothesised that anaemia might be associated with a worse outcome and more bleeding in these anticoagulated patients. SETTING: Data were collected from 17 secondary care centres in Europe. PARTICIPANTS: Consecutive patients with AF undergoing PCI were enrolled in the prospective, multicenter AFCAS (Atrial Fibrillation undergoing Coronary Artery Stenting) registry. Altogether, 929 patients participated in the study. Preprocedural haemoglobin concentration was available for 861 (92.7%; 30% women). The only exclusion criteria were inability or unwillingness to give informed consent. Anaemia was defined as a haemoglobin concentration of <12 g/dL for women and <13 g/dL for men. OUTCOME MEASURES: The primary endpoint was occurrence of major adverse cardiac and cerebrovascular events (MACCE) or bleeding events. RESULTS: 258/861 (30%) patients had anaemia. Anaemic patients were older, more often had diabetes, higher CHA(2)DS(2)-VASc scores, prior history of heart failure, chronic renal impairment and acute coronary syndrome. Anaemic patients had more MACCE than non-anaemic (29.1% vs 19.4%, respectively, p=0.002), and minor bleeding events (7.0% vs 3.3%, respectively, p=0.028), with a trend towards more total bleeding events (25.2% vs 21.7%, respectively, p=0.059). No difference was observed in antithrombotic regimens at discharge. In multivariate analysis, anaemia was an independent predictor of all-cause mortality at 12-month follow-up (hazard ratio 1.62, 95% CI 1.05 to 2.51, p=0.029). CONCLUSIONS: Anaemia was a frequent finding in patients with AF referred for PCI. Anaemic patients had a higher all-cause mortality, more thrombotic events and minor bleeding events. Anaemia seems to be an identification of patients at risk for cardiovascular events and death. TRIAL REGISTRATION: ClinicalTrials.gov number NCT00596570

Arbeitsbezogenes sedentäres Verhalten

Hintergrund Sedentäres Verhalten ist mit einem erhöhten Risiko für chronische Krankheiten und einer höheren Gesamtmortalität assoziiert. Arbeitsbezogenes Sitzen hat einen großen Anteil am täglichen sedentären Verhalten, insbesondere bei Büroangestellten. Ziel der Arbeit In dieser Studie sollte untersucht werden, wie viel Zeit bei verschiedenen Aufgaben am Arbeitsplatz und beim Pendeln von Verwaltungsangestellten einer Universitätsklinik in Deutschland im Sitzen verbracht wird. Material und Methoden Eine fragebogengestützte Querschnittsstudie mit Verwaltungsangestellten des Universitätsklinikums Regensburg wurde durchgeführt, um arbeitsbezogenes sedentäres Verhalten zu untersuchen. Ergebnisse Die Studienpopulation bestand aus 159 Teilnehmenden (54,1 % Frauen, 51,6 % älter als 40 Jahre), was einer Antwortquote von 26 % entspricht. Die durchschnittliche tägliche Sitzzeit am Arbeitsplatz betrug im Median 7,0 h (Interquartilsbereich [IQR] 6,0–7,5 h) und fand hauptsächlich bei der Computerarbeit statt (57,3 %). Die mittlere Stehzeit bei der Arbeit betrug im Median 0,8 h (IQR 0,3–1,4 h). Verwaltungsangestellte verbrachten während des Pendelns im Median 0,7 h (IQR 0,3–1,0 h) pro Tag im Sitzen. Die Teilnehmenden waren der Ansicht, dass langes und ununterbrochenes Sitzen negative (69,6 %) oder eher negative (29,7 %) Auswirkungen auf die Gesundheit hat. Diskussion Verwaltungspersonal in Krankenhäusern verbringt einen großen Teil der täglichen Arbeitszeit mit sedentärem Verhalten. Maßnahmen, die es ermöglichen, sowohl im Sitzen als auch im Stehen zu arbeiten, können zu einer Verringerung der arbeitsbedingten Sitzzeit führen und damit die Gesundheit am Arbeitsplatz und im weiteren Sinne die öffentliche Gesundheit verbessern

Photosynthesis in C3-C4 intermediate Moricandia species.

Evolution of C4 photosynthesis is not distributed evenly in the plant kingdom. Particularly interesting is the situation in the Brassicaceae, because the family contains no C4 species, but several C3-C4 intermediates, mainly in the genus Moricandia Investigation of leaf anatomy, gas exchange parameters, the metabolome, and the transcriptome of two C3-C4 intermediate Moricandia species, M. arvensis and M. suffruticosa, and their close C3 relative M. moricandioides enabled us to unravel the specific C3-C4 characteristics in these Moricandia lines. Reduced CO2 compensation points in these lines were accompanied by anatomical adjustments, such as centripetal concentration of organelles in the bundle sheath, and metabolic adjustments, such as the balancing of C and N metabolism between mesophyll and bundle sheath cells by multiple pathways. Evolution from C3 to C3-C4 intermediacy was probably facilitated first by loss of one copy of the glycine decarboxylase P-protein, followed by dominant activity of a bundle sheath-specific element in its promoter. In contrast to recent models, installation of the C3-C4 pathway was not accompanied by enhanced activity of the C4 cycle. Our results indicate that metabolic limitations connected to N metabolism or anatomical limitations connected to vein density could have constrained evolution of C4 in Moricandia