Competências do orientador profissional brasileiro

As transformações e novas demandas do mundo do trabalho têm protagonizado importantes discussões que repercutem no papel da Orientação Profissional. Tais discussões levaram à preocupação com a formação do orientador profissional, partindo do conceito de competências. Esta atenção foi intensificada a partir do estudo iniciado pela IAEVG, que abordou parâmetros de competências do orientador escolar e profissional no cenário internacional, propondo um modelo de competências centrais e especializadas. Baseado em tal modelo apresenta-se o levantamento das competências profissionais do orientador profissional brasileiro. Foi utilizado o questionário de avaliação de competências internacionais para orientadores escolares e profissionais desenvolvido por Repetto et al. (2004, 2008) e, a partir dos resultados, discutem-se a formação e atuação do orientador no Brasil e implicações para melhor qualidade de seus serviços.Changes and new demands in the world of work have lead an important debate with implications in the role of Vocational Guidance. This debate has generated mayor attention on the concept of vocational counselor competency based training. This attention increased on the bases of the study initiated by the IAEVG, and its international approach of the competence standards, that proposed a model for core and specialized competencies. Based on that model, an adaptation of the professional competencies of the Brazilian vocational counselor is presented here. With this aim, an evaluation questionnaire developed by Repetto et als. (2004, 2008) was used; from the results of this questionnaire, training and work of guidance practitioners and implications for improving the quality of their service is discussed.Las transformaciones y nuevas demandas del mundo del trabajo han protagonizado un importante debate que repercute en el papel que juega la Orientación Profesional. Este debate ha generado mayor atención sobre la formación del orientador profesional, basado en el concepto de competencias. Esta atención se intensificó a partir del estudio iniciado por la IAEVG, que abordó estándares de competencias del orientador escolar y profesional en un escenario internacional, proponiendo un modelo de competencias centrales y especializadas. Basado en tal modelo, se presenta una adaptación a las competencias profesionales del orientador profesional brasileño. Para ello, se utilizó un cuestionario de evaluación de competencias internacionales para orientadores escolares y profesionales desarrollado por Repetto et al. (2004, 2008) y, a partir de los resultados, se discute la formación e intervención del orientador en Brasil y las implicaciones para mejorar la calidad de sus servicios.Fundação para a Ciência e a Tecnologia (FCT) -SFRH/BD/18637/2004-FC

Development of a low-cost copro-LAMP assay for simultaneous copro-detection of Toxocara canis and Toxocara cati

Toxocariasis is a zoonotic disease caused mainly by Toxocara canis and Toxocara cati and diagnosis in dogs and cats is an important tool for its control. For this reason, a new coprological loop-mediated isothermal amplification (LAMP) assay was developed for the simultaneous detection of these species. The primer set was designed on a region of the mitochondrial cox-1 gene. Amplification conditions were evaluated using a temperature gradient (52°C to 68°C), different incubation times (15-120 min), and different concentrations of malachite green dye (0.004-0.4% w/v). The analytical sensitivity was evaluated with serial dilutions of genomic DNA from T. canis and T. cati adult worms, and with serial dilutions of DNA extracted from feces using a low-cost in-house method. The specificity was evaluated using genomic DNA from Canis lupus familiaris, Felis catus, Escherichia coli, Toxascaris leonina, Ancylostoma caninum, Echinococcus granulosus sensu stricto and Taenia hydatigena. The LAMP assay applied to environmental fecal samples from an endemic area showed an analytical sensitivity of 10-100 fg of genomic DNA and 10-5 serial dilutions of DNA extracted from feces using the low-cost in-house method; with a specificity of 100%. Additionally, the total development of the assay was carried out in a basic laboratory and per-reaction reagent cost decreased by ~80%. This new, low-cost tool can help identify the most common agents of toxocariasis in endemic areas in order to manage prevention strategies without having to rely on a laboratory with sophisticated equipment.Fil: Avila, Héctor Gabriel. Universidad Católica de Cuyo. Facultad de Ciencias Químicas y Tecnológicas. Laboratorio Provincial de Zoonosis Provincial; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; ArgentinaFil: Risso, Marikena Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; ArgentinaFil: Ruybal, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; ArgentinaFil: Repetto, Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; ArgentinaFil: Butti, Marcos Javier. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Laboratorio de Parasitosis Humanas y Zoonosis Parasitarias; ArgentinaFil: Trangoni, Marcos David. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; ArgentinaFil: Grune Loffler, Sylvia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología Veterinaria - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Patobiología Veterinaria; ArgentinaFil: Perez, Veronica Mirtha. Gobierno de la Provincia de San Juan. Ministerio de Salud Publica. Direccion de Epidemiologia. Seccion Rabia y Zoonosis.; ArgentinaFil: Periago, Maria Victoria. Fundación Mundo Sano; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin

Evolution of Surface Characteristics of Three Debris-Covered Glaciers in the Patagonian Andes From 1958 to 2020

A number of glaciological observations on debris-covered glaciers around the globe have shown a delayed length and mass adjustment in relation to climate variability, a behavior normally attributed to the ice insulation effect of thick debris layers. Dynamic interactions between debris cover, geometry and surface topography of debris-covered glaciers can nevertheless govern glacier velocities and mass changes over time, with many glaciers exhibiting high thinning rates in spite of thick debris cover. Such interactions are progressively being incorporated into glacier evolution research. In this paper we reconstruct changes in debris-covered area, surface velocities and surface features of three glaciers in the Patagonian Andes over the 1958–2020 period, based on satellite and aerial imagery and Digital Elevation Models. Our results show that debris cover has increased from 40 ± 0.6 to 50 ± 6.7% of the total glacier area since 1958, whilst glacier slope has slightly decreased. The gently sloping tongues have allowed surface flow velocities to remain relatively low (<60 m a−1) for the last two decades, preventing evacuation of surface debris, and contributing to the formation and rise of the ice cliff zone upper boundary. In addition, mapping of end of summer snowline altitudes for the last two decades suggests an increase in the Equilibrium Line Altitudes, which promotes earlier melt out of englacial debris and further increases debris-covered ice area. The strongly negative mass budget of the three investigated glaciers throughout the study period, together with the increases in debris cover extent and ice cliff zones up-glacier, and the low velocities, shows a strong linkage between debris cover, mass balance evolution, surface velocities and topography. Interestingly, the presence of thicker debris layers on the lowermost portions of the glaciers has not lowered thinning rates in these ice areas, indicating that the mass budget is mainly driven by climate variability and calving processes, to which the influence of enhanced thinning at ice cliff location can be added

Competências do Orientador Profissional Brasileiro

Changes and new demands in the world of work have lead an important debate with implications in the role of Vocational Guidance. This debate has generated mayor attention on the concept of vocatio‐ nal counselor competency based training. This attention increased on the bases of the study initiated by the IAEVG, and its international approach of the competence standards, that proposed a model for core and specialized competencies. Based on that model, an adaptation of the professional competencies of the Brazilian vocational counselor is presented here. With this aim, an evaluation questionnaire developed by Repetto et als. (2004, 2008) was used; from the results of this questionnaire, training and work of guidance practitioners and implications for improving the quality of their service is discussed.RESUMENLas transformaciones y nuevas demandas del mundo del trabajo han protagonizado un importante debate que repercute en el papel que juega la Orientación Profesional. Este debate ha generado mayor atención sobre la formación del orientador profesional, basado en el concepto de competencias. Esta atención se intensificó a partir del estudio iniciado por la IAEVG, que abordó estándares de compe‐ tencias del orientador escolar y profesional en un escenario internacional, proponiendo un modelo de competencias centrales y especializadas. Basado en tal modelo, se presenta una adaptación a las com‐ petencias profesionales del orientador profesional brasileño. Para ello, se utilizó un cuestionario de evaluación de competencias internacionales para orientadores escolares y profesionales desarrollado por Repetto et al. (2004, 2008) y, a partir de los resultados, se discute la formación e intervención del orientador en Brasil y las implicaciones para mejorar la calidad de sus servicios.RESUMOAs transformações e novas demandas do mundo do trabalho têm protagonizado importantes discussões que repercutem no papel da Orientação Profissional. Tais discussões levaram à preocupação com a formação do orientador profissional, partindo do conceito de competências. Esta atenção foi intensificada a partir do estudo iniciado pela IAEVG, que abordou parâmetros de competências do orienta‐ dor escolar e profissional no cenário internacional, propondo um modelo de competências centrais e especializadas. Baseado em tal modelo apresenta-se o levantamento das competências profissionais do orientador profissional brasileiro. Foi utilizado o questionário de avaliação de competências internacionais para orientadores escolares e profissionais desenvolvido por Repetto et al. (2004, 2008) e, a partir dos resultados, discutemse a formação e atuação do orientador no Brasil e implicações para melhor qualidade de seus serviços. ABSTRACT Changes and new demands in the world of work have lead an important debate with implications in the role of Vocational Guidance. This debate has generated mayor attention on the concept of vocatio‐ nal counselor competency based training. This attention increased on the bases of the study initiated by the IAEVG, and its international approach of the competence standards, that proposed a model for core and specialized competencies. Based on that model, an adaptation of the professional competencies of the Brazilian vocational counselor is presented here. With this aim, an evaluation questionnaire developed by Repetto et als. (2004, 2008) was used; from the results of this questionnaire, training and work of guidance practitioners and implications for improving the quality of their service is discussed.As transformações e novas demandas do mundo do trabalho têm protagonizado importantes discussões que repercutem no papel da Orientação Profissional. Tais discussões levaram à preocupação com a formação do orientador profissional, partindo do conceito de competências. Esta atenção foi intensificada a partir do estudo iniciado pela IAEVG, que abordou parâmetros de competências do orienta‐ dor escolar e profissional no cenário internacional, propondo um modelo de competências centrais e especializadas. Baseado em tal modelo apresenta-se o levantamento das competências profissionais do orientador profissional brasileiro. Foi utilizado o questionário de avaliação de competências internacionais para orientadores escolares e profissionais desenvolvido por Repetto et al. (2004, 2008) e, a partir dos resultados, discutemse a formação e atuação do orientador no Brasil e implicações para melhor qualidade de seus serviços

First identification of Strongyloides stercoralis infection in a pet dog in Argentina, using integrated diagnostic approaches

Background: Strongyloides stercoralis is a soil-transmitted intestinal nematode with a complex life cycle that primarily affects humans, non-human primates, dogs, and occasionally cats. This study presents, to the best of our knowledge, the first case of S. stercoralis infection and its genotyping in a domestic dog from Argentina. Methods: The patient was a female wired-haired Teckel dog exhibiting recurrent coughing. Coproparasitological analysis using the Baermann technique revealed the presence of rhabditiform larvae morphologically compatible with S. stercoralis. To confirm this finding, molecular diagnosis (18S ribosomal RNA) and analysis of the cox1 gene were performed. Results: We identified a haplotype (HP20) that has previously only been related to S. stercoralis infection in dogs, but was found in the present study to be highly related to the haplotype (HP16) of a zoonotic variant and divergent from those previously described from human patients in Argentina. Furthermore, unlike in human cases following treatment with ivermectin, the dog was negative after moxidectin treatment according to polymerase chain reaction of the sampled faeces. Conclusions: This case report shows the importance of further investigation into potential transmission events and prevalences of S. stercoralis in dogs and humans in South America. The results reported here should also encourage future work that examines different scenarios of infection with S. stercoralis in dogs and humans with the aim of integrating clinical management, diagnosis, treatment and follow-up strategies in the quest for new approaches for the treatment of this disease in animals and humans. The findings support the adoption of a One Health approach, which recognizes the interconnectedness between animal and human health, in addressing parasitic infections such as strongyloidiasis

Benznidazole biotransformation and multiple targets in <i>Trypanosoma</i> cruzi revealed by metabolomics

&lt;b&gt;Background&lt;/b&gt;&lt;p&gt;&lt;/p&gt; The first line treatment for Chagas disease, a neglected tropical disease caused by the protozoan parasite Trypanosoma cruzi, involves administration of benznidazole (Bzn). Bzn is a 2-nitroimidazole pro-drug which requires nitroreduction to become active, although its mode of action is not fully understood. In the present work we used a non-targeted MS-based metabolomics approach to study the metabolic response of T. cruzi to Bzn.&lt;p&gt;&lt;/p&gt; &lt;b&gt;Methodology/Principal findings&lt;/b&gt;&lt;p&gt;&lt;/p&gt; Parasites treated with Bzn were minimally altered compared to untreated trypanosomes, although the redox active thiols trypanothione, homotrypanothione and cysteine were significantly diminished in abundance post-treatment. In addition, multiple Bzn-derived metabolites were detected after treatment. These metabolites included reduction products, fragments and covalent adducts of reduced Bzn linked to each of the major low molecular weight thiols: trypanothione, glutathione, γ-glutamylcysteine, glutathionylspermidine, cysteine and ovothiol A. Bzn products known to be generated in vitro by the unusual trypanosomal nitroreductase, TcNTRI, were found within the parasites, but low molecular weight adducts of glyoxal, a proposed toxic end-product of NTRI Bzn metabolism, were not detected.&lt;p&gt;&lt;/p&gt; &lt;b&gt;Conclusions/significance&lt;/b&gt;&lt;p&gt;&lt;/p&gt; Our data is indicative of a major role of the thiol binding capacity of Bzn reduction products in the mechanism of Bzn toxicity against T. cruzi

Conjugation with L, L-diphenylalanine Self-Assemblies Enhances In Vitro Antitumor Activity of Phthalocyanine Photosensitizer

We present the synthesis and characterization of new peptide conjugates obtained by hierarchical co-assembly of L,L-diphenylalanine (FF) and zinc phthalocyanine complexes (ZnPc) in water. Self-assembly capabilities under defined conditions were investigated by scanning electron microscopy, and photophysical properties were evaluated using UV-Vis and fluorescence spectroscopy. AFM observations demonstrated that these ZnPcs form different highly ordered arrays on the crystalline faces of the FF microplates and that surface roughness significantly changes with the presence of differently substituted phthalocyanine units. XRD assays showed that the overall molecular packing of the conjugates is organized according to a hexagonal symmetry, with ZnPcs hosted in the interstices of the peptide phase. In vitro photodynamic studies were conducted on human breast cancer MCF-7 cells to investigate both cellular uptake and cytotoxicity. It was shown that FF self-assemblies are not toxicity and enhance accumulation of ZnPc in MCF-7 cells, improving apoptotic cell death upon irradiation. Our findings demonstrate enhancement of ZnPc antitumor efficiency by FF conjugates and a proof-of-concept for new photosensitizer carriers based on peptide conjugates

La investigación universitaria sobre educación : dilemas y prácticas

Se presentan los resultados de investigaciones realizadas en un contexto institucional: la Facultad de Educación Elemental y Especial (FEEYE), de la Universidad Nacional de Cuyo durante el bienio 2011-2013 y son resultado de los proyectos subsidiados por la Secretaría de Ciencia, Técnica y Posgrado de la misma Universidad a partir del programa de incentivo a docentes investigadores o subsidiados por la unidad académica

COVID Feel Good-An Easy Self-Help Virtual Reality Protocol to Overcome the Psychological Burden of Coronavirus

Background: Living in the time of the COVID-19 means experiencing not only a global health emergency but also extreme psychological stress with potential emotional side effects such as sadness, grief, irritability, and mood swings. Crucially, lockdown and confinement measures isolate people who become the first and the only ones in charge of their own mental health: people are left alone facing a novel and potentially lethal situation, and, at the same time, they need to develop adaptive strategies to face it, at home. In this view, easy-to-use, inexpensive, and scientifically validated self-help solutions aiming to reduce the psychological burden of coronavirus are extremely necessary. Aims: This pragmatic trial aims to provide the evidence that a weekly self-help virtual reality (VR) protocol can help overcome the psychological burden of the Coronavirus by relieving anxiety, improving well-being, and reinforcing social connectedness. The protocol will be based on the 'Secret Garden' 360 VR video online (www.covidfeelgood.com) which simulates a natural environment aiming to promote relaxation and self-reflection. Three hundred sixty-degree or spherical videos allow the user to control the viewing direction. In this way, the user can explore the content from any angle like a panorama and experience presence and immersion. The 'Secret Garden' video is combined with daily exercises that are designed to be experienced with another person (not necessarily physically together), to facilitate a process of critical examination and eventual revision of core assumptions and beliefs related to personal identity, relationships, and goals. Methods: This is a multicentric, pragmatic pilot randomized controlled trial involving individuals who experienced the COVID-19 pandemic and underwent a lockdown and quarantine procedures. The trial is approved by the Ethics Committee of the Istituto Auxologico Italiano. Each research group in all the countries joining the pragmatic trial, aims at enrolling at least 30 individuals in the experimental group experiencing the self-help protocol, and 30 in the control group, over a period of 3 months to verify the feasibility of the intervention. Conclusion: The goal of this protocol is for VR to become the 'surgical mask' of mental health treatment. Although surgical masks do not provide the wearer with a reliable level of protection against the coronavirus compared with FFP2 or FFP3 masks, surgical masks are very effective in protecting others from the wearer's respiratory emissions. The goal of the VR protocol is the same: not necessarily to solve complex mental health problems but rather to improve well-being and preserve social connectedness through the beneficial social effects generated by positive emotions