Classifying Single Stars and Spectroscopic Binaries Using Optical Stellar Templates

Stellar spectral classification is a fundamental tool of modern astronomy, providing insight into physical characteristics such as effective temperature, surface gravity, and metallicity. Accurate and fast spectral typing is an integral need for large all-sky spectroscopic surveys like the SDSS and LAMOST. Here, we present the next version of PyHammer, stellar spectral classification software that uses optical spectral templates and spectral line index measurements. PyHammer v2.0 extends the classification power to include carbon (C) stars, DA white dwarf (WD) stars, and also double-lined spectroscopic binaries (SB2). This release also includes a new empirical library of luminosity-normalized spectra that can be used to flux calibrate observed spectra, or to create synthetic SB2 spectra. We have generated physically reasonable SB2 combinations as templates, adding to PyHammer the ability to spectrally type SB2s. We test classification success rates on SB2 spectra, generated from the SDSS, across a wide range of spectral types and signal-to-noise ratios. Within the defined range of pairings described, more than $95\%$ of SB2s are correctly classified.Comment: 16 pages, 7 figures, 4 tables; accepted to ApJ

IRAM and Gaia views of multi-episodic star formation in IC1396A:The origin and dynamics of the Class 0 protostar at the edge of an HII region

(Abridged) IC1396A is a cometary globule containing the Class 0 source IC1396A-PACS-1. We use IRAM 30m and Gaia DR2 data to explore the star-formation history of IC1396A and investigate the possibilities of triggered star formation. IRAM and Herschel continuum data reveal dust temperatures and column densities. Heterodyne data reveal the velocity structure of the gas. Gaia DR2 proper motions for the stars complete the kinematics of the region. IC1396A-PACS-1 shows molecular emission similar to a hot corino with warm carbon chain chemistry, and is surrounded by gas at velocities significantly different from the velocities of the Tr37 cluster. Combining the velocity, column density, and temperature information and Gaia DR2 kinematics, we confirm that IC1396A has suffered various episodes of star formation. IC1396A-PACS-1 is probably the last intermediate-mass protostar that will form within IC1396A, showing evidence of triggering by radiative driven implosion. Chemical signatures place IC1396A-PACS-1 among the youngest protostars known. Gaia DR2 data reveal velocities in the plane of the sky $sim$4km/s for IC1396A with respect to Tr37. The total velocity difference (8 km/s) between the Tr37 cluster and IC1396A is too small for IC1396A to have undergone substantial rocket acceleration, which imposes constraints on the distance to the ionizing source in time and the possibilities of triggered star formation. The three stellar populations in the globule reveal that objects located within relatively close distances (<0.5pc) can be formed in various episodes within a $sim$1-2 Myr period. We expect substantial differences in initial conditions for the resulting objects and their protoplanetary disks, which may affect their evolution. Finally, evidence for short-range feedback from the embedded protostars and, in particular, the A-type star V390 Cep is also observed

The Effect of Dietary Nitrate and Anthocyanins on Anaerobic Exercise Performance

Background: Previously, beetroot and tart cherry supplementations have been investigated for their effects on exercise performance and recovery separately. Beetroot containing nitrate has been used to improve blood flow to enhance exercise tolerance. Tart cherry extract containing anthocyanins has been shown to lower inflammation and improve recovery time in exercise performance. Objective: The purpose of this study was to investigate a possible synergistic effect of nitrate and anthocyanins on exercise performance in healthy individuals. Design: This was a double-blind, randomized crossover study with a duration of twenty-nine days. Inclusion criteria included healthy individuals, able to exercise on a stationary bike, and willing to abstain from caffeine, mouthwash, and high antioxidant foods. Exclusion criteria included orthopedic injuries, use of nonsteroidal anti-inflammatory drugs (NSAIDs), known allergy to tart cherry and/or beetroot, and pregnant or breastfeeding. Participants: Healthy men (n=6) and women (n=23), ranging between 19 and 35 years of age. Participants were recruited from Loma Linda University and surrounding cities. Intervention: Participants were randomized into two groups after baseline. Treatment A was supplemented with 5.05g (3.62 mmol) of beetroot powder and placebo. Treatment B was supplemented with 5.05g (3.62 mmol) of beetroot powder and 480 mg tart cherry capsule for seven-days, followed by a seven-day washout period. Treatment was alternated at the end of the 14-day period. Main outcomes: exercise performance (heart rate, speed, and distance) Secondary outcomes: serum lactate and allantoin Statistics: A mixed model analysis was used to measure changes in continuous variables. Mean and standard deviation were calculated for treatment A and treatment B. Results: During the first five seconds of the 30 second sprint, heart rate of treatment A was significantly lower than treatment B. Difference in heart rate ranged from 1.6 to 2.7 BPM. Participants in treatment B pedaled five meters farther, than treatment A. This outcome was not statistically significant. No significant differences were observed between both treatments on rpm, blood pressure, lactate, and allantoin. Conclusion: Supplementation with beetroot powder and tart cherry for seven days may synergistically improve anaerobic exercise performance by maximizing potential heart rate and energy output

High variability of Blue Carbon storage in seagrass meadows at the estuary scale

Seagrass meadows are considered important natural carbon sinks due to their capacity to store organic carbon (Corg) in sediments. However, the spatial heterogeneity of carbon storage in seagrass sediments needs to be better understood to improve accuracy of blue carbon assessments, particularly when strong gradients are present. We performed an intensive coring study within a sub-tropical estuary to assess the spatial variability in sedimentary Corg associated with seagrasses, and to identify the key factors promoting this variability. We found a strong spatial pattern within the estuary, from 52.16 mg Corg cm-3 in seagrass meadows in the upper parts, declining to 1.06 mg Corg cm-3 in seagrass meadows at the estuary mouth, despite a general gradient of increasing seagrass cover and seagrass habitat extent in the opposite direction. The sedimentary Corg underneath seagrass meadows came principally from allochthonous (non-seagrass) sources (~70-90%), while the contribution of seagrasses was low (~10-30%) throughout the entire estuary. Our results showed that Corg stored in sediments of seagrass meadows can be highly variable within an estuary, attributed largely to accumulation of fine sediments and inputs of allochthonous sources. Local features and the existence of spatial gradients must be considered in blue carbon estimates in coastal ecosystems

Aspects of the Release of Superoxide by Leukocytes, and a Means by Which This Is Switched Off

Although great progress has been made in understanding the respiratory burst of leukocytes that produce superoxide (O2-), it is possible that a component or components, might have been overlooked. Furthermore, O2- production and its sequels, though cardinal in bactericidal action, might ultimately be damaging to the host's own cells. It is important, therefore, that a biologic mechanism exist to turn off O2- production by stimulated leukocytes. This article offers evidence that methoxatin (PQQ), a redox-cycling orthoquinone, might be involved in O2- production by leukocytes. This is based on the fact that inhibitors of O2- production, such as diphenylene iodonium (DPI) and 4,5-dimethylphenylene diamine (DIMPDA), were shown to sequester PQQ in leukocytes, i.e., to form adducts with that substance. Addition of PQQ to cells blocked with the inhibitors partially restored O2- release. With respect to turning off cellular O2- release, a factor was observed to be released to the medium by old macrophages (14 days old, but not by those less than 7 days old). Such conditioned medium, when added to stimulated neutrophils or macrophages, blocked O2- release. This factor was sensitive to proteases, exhibited molecular sizes of 3 and 11 kDa, and its action was independent of the nature of the stimulus applied to the leukocytes. It was partially purified by column (sizing) chromatography and HPLC. It seems to be a general modulator of the release of reactive oxygen species by phagocytes and is irrespective of phagocytic cellular type, or species from which the cells were derived

Fragile, unfaithful and persistent Ys-on how meiosis can shape sex chromosome evolution

Sex-linked inheritance is a stark exception to Mendel's Laws of Heredity. Here we discuss how the evolution of heteromorphic sex chromosomes (mainly the Y) has been shaped by the intricacies of the meiotic programme. We propose that persistence of Y chromosomes in distantly related mammalian phylogroups can be explained in the context of pseudoautosomal region (PAR) size, meiotic pairing strategies, and the presence of Y-borne executioner genes that regulate meiotic sex chromosome inactivation. We hypothesise that variation in PAR size can be an important driver for the evolution of recombination frequencies genome wide, imposing constraints on Y fate. If small PAR size compromises XY segregation during male meiosis, the stress of producing aneuploid gametes could drive function away from the Y (i.e., a fragile Y). The Y chromosome can avoid fragility either by acquiring an achiasmatic meiotic XY pairing strategy to reduce aneuploid gamete production, or gain meiotic executioner protection (a persistent Y). Persistent Ys will then be under strong pressure to maintain high recombination rates in the PAR (and subsequently genome wide), as improper segregation has fatal consequences for germ cells. In the event that executioner protection is lost, the Y chromosome can be maintained in the population by either PAR rejuvenation (extension by addition of autosome material) or gaining achiasmatic meiotic pairing, the alternative is Y loss. Under this dynamic cyclic evolutionary scenario, understanding the meiotic programme in vertebrate and invertebrate species will be crucial to further understand the plasticity of the rise and fall of heteromorphic sex chromosomes

Altered 2-thiouridylation impairs mitochondrial translation in reversible infantile respiratory chain deficiency.

Childhood-onset mitochondrial encephalomyopathies are severe, relentlessly progressive conditions. However, reversible infantile respiratory chain deficiency (RIRCD), due to a homoplasmic mt-tRNA(Glu) mutation, and reversible infantile hepatopathy, due to tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase (TRMU) deficiency, stand out by showing spontaneous recovery, and provide the key to treatments of potential broader relevance. Modification of mt-tRNA(Glu) is a possible functional link between these two conditions, since TRMU is responsible for 2-thiouridylation of mt-tRNA(Glu), mt-tRNA(Lys) and mt-tRNA(Gln). Here we show that down-regulation of TRMU in RIRCD impairs 2-thiouridylation and exacerbates the effect of the mt-tRNA(Glu) mutation by triggering a mitochondrial translation defect in vitro. Skeletal muscle of RIRCD patients in the symptomatic phase showed significantly reduced 2-thiouridylation. Supplementation with l-cysteine, which is required for optimal TRMU function, rescued respiratory chain enzyme activities in human cell lines of patients with RIRCD as well as deficient TRMU. Our results show that l-cysteine supplementation is a potential treatment for RIRCD and for TRMU deficiency, and is likely to have broader application for the growing group of intra-mitochondrial translation disorders