Influence network linkages across implementation strategy conditions in a randomized controlled trial of two strategies for scaling up evidence-based practices in public youth-serving systems.

BackgroundGiven the importance of influence networks in the implementation of evidence-based practices and interventions, it is unclear whether such networks continue to operate as sources of information and advice when they are segmented and disrupted by randomization to different implementation strategy conditions. The present study examines the linkages across implementation strategy conditions of social influence networks of leaders of youth-serving systems in 12 California counties participating in a randomized controlled trial of community development teams (CDTs) to scale up use of an evidence-based practice.MethodsSemi-structured interviews were conducted with 38 directors, assistant directors, and program managers of county probation, mental health, and child welfare departments. A web-based survey collected additional quantitative data on information and advice networks of study participants. A mixed-methods approach to data analysis was used to create a sociometric data set (n = 176) to examine linkages between treatment and standard conditions.ResultsOf those network members who were affiliated with a county (n = 137), only 6 (4.4%) were directly connected to a member of the opposite implementation strategy condition; 19 (13.9%) were connected by two steps or fewer to a member of the opposite implementation strategy condition; 64 (46.7%) were connected by three or fewer steps to a member of the opposite implementation strategy condition. Most of the indirect steps between individuals who were in different implementation strategy conditions were connections involving a third non-county organizational entity that had an important role in the trial in keeping the implementation strategy conditions separate. When these entities were excluded, the CDT network exhibited fewer components and significantly higher betweenness centralization than did the standard condition network.ConclusionAlthough the integrity of the RCT in this instance was not compromised by study participant influence networks, RCT designs should consider how influence networks may extend beyond boundaries established by the randomization process in implementation studies.Trial registrationNCT00880126

Avaliação da síntese de lipídeo pela levedura Candida zeylanoides QU 33 em meio de cultura com glicose e sulfato de amônio

Microbial oil can be considered a source of monounsaturated (MUFAs) and polyunsaturated (PUFAs) fatty acids. Previous research has shown that the yeast Candida zeylanoides QU 33 produces large amounts of MUFAs and PUFAs in culture media containing glucose as carbon source and (NH4)2SO4 as nitrogen source. This study aimed to determine the best parameters for the accumulation of lipids by C. zeylanoides QU 33, knowing that the carbon-to-nitrogen ratio (C:N) in the culture media, as well as pH and temperature, are the parameters that most affect the production of microbial lipids. For optimization, the Response Surface Methodology was applied, using levels of the variables temperature (20-28 ºC), pH (6.0 to 8.0), and glucose concentration (08-20 % w/v). Although no variable showed significant influence, there was a tendency towards lipid accumulation under conditions of high sugar concentrations (above 16%), mid temperatures (23-26º C) and neutral pH.Óleo microbiano pode ser considerado uma fonte de ácidos graxos monoinsaturados (MUFAs) e poli-insaturados (PUFAs). Em trabalhos prévios, foi demonstrado que a levedura Candida zeylanoides QU 33 produziu uma grande quantidade de MUFAs e PUFAs em meio de cultura contendo glicose como fonte de carbono e (NH4)2SO4 como fonte de nitrogênio. O presente trabalho objetivou determinar os melhores parâmetros para o acúmulo de lipídeos pela levedura C. zeylanoides QU 33, sabendo-se que a razão carbono nitrogênio (C:N) nos meios de cultivo, assim como o pH e a temperatura de incubação são os parâmetros que mais influenciam a produção de lipídios microbianos. Para a otimização, foi aplicada a Metodologia de Superfície de Resposta, aplicando os níveis das variáveis temperatura (20 a 28 ºC), pH (6,0 a 8,0) e concentração de glicose (8 a 20 % p/v). Apesar de nenhuma das variáveis ter tido influência significativa, houve tendência a um maior acúmulo de lipídeos em altas concentrações do açúcar (acima de 16%), temperaturas medianas (23-26ºC) e pH neutro

Hydrogeochemistry and distribution of potentially toxic elements in a metallogenic province – The Iberian Pyrite Belt, Portugal

Contamination by acid drainage is an environmental problem in mineralized regions, especially in the surroundings of sulphide mines. The water rock interaction process involves the oxidative dissolution of sulphides, naturally or by mining activity, that generates acidity which, in turn, produces sulfate, and water pollution by sulphide-hosted metals. The particular geology of the Iberian Pyrite Belt (IPB) in the southwestern Iberian Peninsula creates the ideal conditions for such water contamination. Different water types were sampled at 28 locations across the entire IBP metallogenetic province. Anions, metals, and arsenic were analysed to assess the influence of acid drainage. The results demonstrate the sulfate nature of waters in the vicinity of mines. Other types of water in the IPB region are mainly mixed chloride and bicarbonate (river and groundwater) and mixed and sodium-bicarbonate (lakes) types. Water quality assessment indicated strong contamination of surface waters that are directly influenced by mine wastes. There is the additional concern that some lakes and groundwaters exhibit concentrations of potentially toxic elements (e.g. Al, As) that are above the regulatory limits established by the European Commission Water Framework Directive.This work was co-funded by the European Union through the European Regional Development Fund, based on COMPETE 2020 - project ICT (UID/GEO/04683/2013) with reference POCI-01-0145-FEDER-007690 and project Nano-MINENV number 029259

Population structure of Annona coriacea Mart. (Annonaceae) in different Cerrado phytophysiognomies

Knowing the regeneration and structure of a species in different phytophysiognomies is extremely important to understand its pattern of occurrence. In spite of its importance, this type of study is still scarce in the Cerrado biome. In this perspective, this work aimed to compare the structure of the arboreal and regenerating strata of Annona coriacea in the cerrado sensu stricto and in the cerradão (savanna woodland). For that purpose, 13 plots of 400m² were installed in each physiognomy. All individuals of A. coriacea were sampled and had their diameter and height measured. The individuals were divided into regenerating and arboreal and were distributed in classes of height and diameter. Furthermore, the absolute density and the basal area of each extract were calculated in each physiognomy. The parameters were compared using the T-test. In total, 130 individuals of A. Coriacea were sampled, with 42 regenerating individuals in the cerrado sensu stricto and 49 in the cerradão. As for the arboreal individuals, 33 were sampled in the cerrado sensu stricto and 6 in the cerradão. All evaluated parameters revealed to be significantly different for the physiognomies. Based on these results, it is possible to affirm that A. coriacea did not present a distribution pattern in the two physiognomies, although it presents a better establishment success in the cerrado sensu stricto. In the cerradão, although the seeds can germinate, the seedlings fail to develop and reach the adult age.Knowing the regeneration and structure of a species in different phytophysiognomies is extremely important to understand its pattern of occurrence. In spite of its importance, this type of study is still scarce in the Cerrado biome. In this perspective, this work aimed to compare the structure of the arboreal and regenerating strata of Annona coriacea in the cerrado sensu stricto and in the cerradão (savanna woodland). For that purpose, 13 plots of 400m² were installed in each physiognomy. All individuals of A. coriacea were sampled and had their diameter and height measured. The individuals were divided into regenerating and arboreal and were distributed in classes of height and diameter. Furthermore, the absolute density and the basal area of each extract were calculated in each physiognomy. The parameters were compared using the T-test. In total, 130 individuals of A. Coriacea were sampled, with 42 regenerating individuals in the cerrado sensu stricto and 49 in the cerradão. As for the arboreal individuals, 33 were sampled in the cerrado sensu stricto and 6 in the cerradão. All evaluated parameters revealed to be significantly different for the physiognomies. Based on these results, it is possible to affirm that A. coriacea did not present a distribution pattern in the two physiognomies, although it presents a better establishment success in the cerrado sensu stricto. In the cerradão, although the seeds can germinate, the seedlings fail to develop and reach the adult age

Acid Mine Drainage Precipitates at the Nanometric Scale – Properties and Environmental Role

The mineral-water interactions responsible for mobilization of dissolved toxic elements in mine drainage often generate colloids that commonly occur at the nanometric scale. This study presents typical properties of these materials, mostly composed by iron-rich products. The samples were obtained in a variety of contexts, representing mine waters as well as natural acid rock drainage. It concludes by noting the potential influence of the waste and/or the host rocks on the hydrogeochemistry of the systems. Further, the water properties could control the morphology and mineralogy of this very fine material, and consequently its environmental role

Memory In Children With Symptomatic Temporal Lobe Epilepsy.

In children with temporal lobe epilepsy (TLE), memory deficit is not so well understood as it is in adults. The aim of this study was to identify and describe memory deficits in children with symptomatic TLE, and to verify the influence of epilepsy variables on memory. We evaluated 25 children with TLE diagnosed on clinical, EEG and MRI findings. Twenty-five normal children were compared with the patients. All children underwent a neuropsychological assessment to estimate intellectual level, attention, visual perception, handedness, and memory processes (verbal and visual: short-term memory, learning, and delayed recall). The results allowed us to conclude: besides memory deficits, other neuropsychological disturbances may be found in children with TLE such as attention, even in the absence of overall cognitive deficit; the earlier onset of epilepsy, the worse verbal stimuli storage; mesial lesions correlate with impairment in memory storage stage while neocortical temporal lesions correlate with retrieval deficits.72184-

Análise psicométrica e dados normativos da UWES em adolescentes peruanos

Academic engagement is a relevant characteristic to predict successful academic trajectories and school performance; however, there is a lack of validated instruments in Peru for its evaluation. Therefore, the objective of this study was to analyze the psychometric properties of the Utrecht Work Engagement Scale – 9S (UWES-9S) in Peruvian adolescents: internal structure, measurement invariance, association with academic self-efficacy, reliability, and norms. A total of 868 school adolescents (51.728% women; Mage = 14.263; SDage = 1.430) from six schools in the Constitutional Province of Callao (central coast of Peru) were evaluated. In addition to the UWES-9S, the Specific Perceived Self-Efficacy Scale for Academic Situations was used as a measure of self-efficacy. The results show that the UWES-9S is unidimensional and invariant between men and women, although three items were eliminated, resulting in a new version: UWES-6S. Likewise, the association with academic self-efficacy was moderate (r > .50) and norms were obtained for men and women separately. In addition, the reliability indices were satisfactory for both the scores (alpha coefficient and average inter-item correlation) and the construct (omega coefficient > .80). It is concluded that the UWES-6S has adequate psychometric properties for its application in Peruvian adolescents.El engagement académico es una característica relevante para predecir las trayectorias académicas exitosas y el rendimiento escolar; sin embargo, existe una carencia de instrumentos validados en Perú para su evaluación. Por ello, el objetivo de esta investigación fue analizar las propiedades psicométricas de la Utrech Work Engagement Scale – 9S (UWES-9S) en adolescentes peruanos: estructura interna, invarianza de medición, asociación con la autoeficacia académica, confiabilidad y normas. Fueron evaluados 868 adolescentes escolares (51.728% mujeres; Medad = 14.263; DEedad = 1.430) procedentes de seis colegios de la Provincia Constitucional del Callao (costa central del Perú). Además de la UWES-9S se utilizó la Escala de Autoeficacia Percibida Específica para Situaciones Académicas como medida de autoeficacia. Los resultados indican que la UWES-9S es unidimensional e invariante entre hombres y mujeres, aunque se eliminaron tres ítems derivando en una nueva versión: UWES-6S. Asimismo, la asociación con la medida de autoeficacia académica fue moderada (r > .50) y se obtuvo normas para hombres y mujeres por separado. Los indicadores de confiabilidad fueron satisfactorios tanto para las puntuaciones (coeficiente alfa y correlación inter-ítem promedio) como para el constructo (coeficiente omega > .80). Se concluye que la UWES-6S cuenta con propiedades psicométricas adecuadas para su aplicación en adolescentes peruanos.O engagement académico é uma característica relevante para prever trajetórias académicas bem-sucedidas e o desempenho escolar, no entanto, faltam instrumentos validados no Perú para a sua avaliação. Portanto, o objetivo deste estudo foi analisar as propriedades psicométricas da Utrecht Work Engagement Scale – 9S (UWES-9S) em adolescentes peruanos: estrutura interna, invariância de medidas, associação com a autoeficácia académica, confiabilidade e normas. Foram avaliados 868 adolescentes escolares (51.728% mulheres; Midade = 14.263; DPidade = 1.430) de seis colégios da Província Constitucional de Callao (costa central do Perú). Além da UWES-9S, foi utilizada a Escala de Autoeficácia Percebida Específica para Situações Académicas como medida de autoeficácia. Os resultados mostram que a UWES-9S é unidimensional e invariável entre homens e mulheres, embora três itens tenham sido eliminados, resultando numa nova versão: UWES-6S. A associação com a autoeficácia académica foi moderada (r > 0,50) e as normas foram obtidas para homens e mulheres separadamente. Além disso, os índices de confiabilidade foram satisfatórios tanto para as pontuações (coeficiente alfa e correlação média entre itens) quanto para o constructo (coeficiente ómega > .80). Conclui-se que a UWES-6S possui propriedades psicométricas adequadas para sua aplicação em adolescentes peruanos

Prevalence, antifungal susceptibility and virulence determinants of oral yeast species isolated from immunodeficient patients in Northeastern Brazil

Background and Objectives: Oral candidiasis has a common occurrence in immunocompromised patients. However, other emergent infections have become increasingly common. The aim of this study was to investigate the prevalence, virulence determinants and the antifungal susceptibility of yeast colonizing the mucosa of immunocompromised patients in Northeastern Brazil. Methods: Samples from sixty HIV-positive patients seen at the Specialized Service / Hospital Dia - Hospital Universitário Prof. Alberto Antunes from the Federal University of Alagoas were collected from subgingival sites and seeded on CHROMagar for presumptive confirmation of Candida spp. followed by PCR and sequencing. In addition, we tested virulence determinants, phospholipase and protease and evaluated in vitro the Minimum Inhibitory Concentration of antifungals amphotericin B and fluconazole. This project was approved by the Research Ethics Committee of the Center for Higher Studies in Maceió. Results: Approximately 63% of the patients were colonized by yeasts, with C. albicans as the predominant species, while non-Candida albicans species accounted for 49% of the isolates, with C. dubliniensis and C. parapsilosis being the commonest, but C. intermedia, Bullera penniseticola and Naganishia liquefaciens were also found. The virulence determinants protease and/or phospholipase were also produced by Candida spp. and some uncommon opportunistic isolates such as Kodamaea ohmeri, N. liquefaciens and Saitozyma podzolica. Furthermore, most of Candida spp. strains and some uncommon opportunistic species showed high values of minimal inhibitory concentration. Conclusion: Results obtained indicate that C. albicans continues to be the predominant species in oral cavity of immunodeficient patients and along with other unusual species may present high resistance to the antifungals tested

Social networks and implementation of evidence-based practices in public youth-serving systems: a mixed-methods study

<p>Abstract</p> <p>Background</p> <p>The present study examines the structure and operation of social networks of information and advice and their role in making decisions as to whether to adopt new evidence-based practices (EBPs) among agency directors and other program professionals in 12 California counties participating in a large randomized controlled trial.</p> <p>Methods</p> <p>Interviews were conducted with 38 directors, assistant directors, and program managers of county probation, mental health, and child welfare departments. Grounded-theory analytic methods were used to identify themes related to EBP adoption and network influences. A web-based survey collected additional quantitative information on members of information and advice networks of study participants. A mixed-methods approach to data analysis was used to create a sociometric data set (n = 176) for examination of associations between advice seeking and network structure.</p> <p>Results</p> <p>Systems leaders develop and maintain networks of information and advice based on roles, responsibility, geography, and friendship ties. Networks expose leaders to information about EBPs and opportunities to adopt EBPs; they also influence decisions to adopt EBPs. Individuals in counties at the same stage of implementation accounted for 83% of all network ties. Networks in counties that decided not to implement a specific EBP had no extra-county ties. Implementation of EBPs at the two-year follow-up was associated with the size of county, urban versus rural counties, and in-degree centrality. Collaboration was viewed as critical to implementing EBPs, especially in small, rural counties where agencies have limited resources on their own.</p> <p>Conclusions</p> <p>Successful implementation of EBPs requires consideration and utilization of existing social networks of high-status systems leaders that often cut across service organizations and their geographic jurisdictions.</p> <p>Trial Registration</p> <p><a href="http://www.clinicaltrials.gov/ct2/show/NCT00880126">NCT00880126</a></p