Guidelines for Reporting and Archiving 210Pb Sediment Chronologies to Improve Fidelity and Extend Data Lifecycle

Radiometric dating methods are essential for developing geochronologies to study Late Quaternary environmental change and 210Pb dating is commonly used to produce age-depth models from recent (within 150 years) sediments and other geoarchives. The past two centuries are marked by rapid environmental socio-ecological changes frequently attributed to anthropogenic land-use activities, modified biogeochemical cycles, and climate change. Consequently, historical reconstructions over this recent time interval have high societal value because analyses of these datasets provide understanding of the consequences of environmental modifications, critical ecosystem thresholds, and to define desirable ranges of variation for management, restoration, and conservation. For this information to be used more broadly, for example to support land management decisions or to contribute data to regional analyses of ecosystem change, authors must report all of the useful age-depth model information. However, at present there are no guidelines for researchers on what information should be reported to ensure 210Pb data are fully disclosed, reproducible, and reusable; leading to a plethora of reporting styles, including inadequate reporting that reduces potential reusability and shortening the data lifecycle. For example, 64% of the publications in a literature review of 210Pb dated geoarchives did not include any presentation of age uncertainty estimates in modeled calendar ages used in age-depth models. Insufficient reporting of methods and results used in 210Pb dating geoarchives severely hampers reproducibility and data reusability, especially in analyses that make use of databased palaeoenvironmental data. Reproducibility of data is fundamental to further analyses of the number of palaeoenvironmental data and the spatial coverage of published geoarchives sites. We suggest, and justify, a set of minimum reporting guidelines for metadata and data reporting for 210Pb dates, including an IEDA (Interdisciplinary Earth Data Alliance), LiPD (Linked Paleo Data) and generic format data presentation templates, to contribute to improvements in data archiving standards and to facilitate the data requirements of researchers analyzing datasets of several palaeoenvironmental study sites. We analyse practices of methods, results and first order interpretation of 210Pb data and make recommendations to authors on effective data reporting and archiving to maximize the value of datasets. We provide empirical evidence from publications and practitioners to support our suggested reporting guidelines. These guidelines increase the scientific value of 210Pb by expanding its relevance in the data lifecycle. Improving quality and fidelity of environmental datasets broadens interdisciplinary use, lengthens the potential lifecycle of data products, and achieves requirements applicable for evidenced-based policy support

Individual Differences in Moral Behaviour: A Role for Response to Risk and Uncertainty?

Investigation of neural and cognitive processes underlying individual variation in moral preferences is underway, with notable similarities emerging between moral- and risk-based decision-making. Here we specifically assessed moral distributive justice preferences and non-moral financial gambling preferences in the same individuals, and report an association between these seemingly disparate forms of decision-making. Moreover, we find this association between distributive justice and risky decision-making exists primarily when the latter is assessed with the Iowa Gambling Task. These findings are consistent with neuroimaging studies of brain function during moral and risky decision-making. This research also constitutes the first replication of a novel experimental measure of distributive justice decision-making, for which individual variation in performance was found. Further examination of decision-making processes across different contexts may lead to an improved understanding of the factors affecting moral behaviour

Deep brain stimulation for Parkinson\u27s disease changes perception in the Rubber Hand Illusion

Parkinson\u27s disease (PD) alters cortico-basal ganglia-thalamic circuitry and susceptibility to an illusion of bodily awareness, the Rubber Hand Illusion (RHI). Bodily awareness is thought to result from multisensory integration in a predominantly cortical network; the role of subcortical connections is unknown. We studied the effect of modulating cortico-subcortical circuitry on multisensory integration for bodily awareness in 24 PD patients treated with subthalamic nucleus (STN) deep brain stimulation (DBS), in comparison to 21 healthy volunteers, using the RHI experiment. Typically, synchronous visuo-tactile cues induce a false perception of touch on the rubber hand as if it were the subject\u27s hand, whereas asynchronous visuo-tactile cues do not. However, we found that in the asynchronous condition, patients in the off-stimulation state did not reject the RHI as strongly as healthy controls; patients\u27 rejection of the RHI strengthened when STN-DBS was switched on, although it remained weaker than that of controls. Patients in the off-stimulation state also misjudged the position of their hand, indicating it to be closer to the rubber hand than controls. However, STN-DBS did not affect proprioceptive judgements or subsequent arm movements altered by the perceptual effects of the illusion. Our findings support the idea that the STN and subcortical connections have a key role in multisensory integration for bodily awareness. Decision-making in multisensory bodily illusions is discussed

Sustainability of beef production from brigalow lands after cultivation and mining. 1. Sown pasture growth and carrying capacity

Context: New Acland coal mine in south-eastern Queensland is seeking to rehabilitate mined land to pastures that are safe, stable and sustainable for beef production. Little is known of the productivity and sustainability of grazing previously mined land in the Darling Downs study region. Additionally, information is required to specify management guidelines for sustainable grazing of regional land types retired from cultivation. Aims: Identify pasture growth characteristics, rainfall use efficiencies and long-term carrying capacities of subtropical sown pastures established on lands rehabilitated after open-cut coal mining in comparison to sown pastures established on un-mined but previously cultivated lands. Methods: Pasture growth and quality (% nitrogen) were observed using the Swiftsynd methodology in ungrazed exclosures with three sites on rehabilitated lands of the Acland Grazing Trial over a 5-year period (2014–2018), and 13 sites on unmined lands over periods of 2–5 years providing data for modelling pasture growth. Key results: Peak pasture yield (TSDM for autumn harvests) averaged for 2017 and 2018 was greater (P < 0.1) on rehabilitated sites than unmined Poplar Box land type sites (5957 and 2233 kg/ha respectively) but similar to Brigalow Uplands and Mountain Coolibah land type sites (3946 and 3413 kg/ha respectively). Pasture rundown was evident, with pasture N uptake decreasing over 5 years at some sites. Soil mineral N supply (potentially mineralisable N and mineral N) in spring was a useful indicator of N uptake over the following growing season. Simulations using the GRASP pasture growth model for the grazing trial period predicted rainfall use efficiencies of 12.0, 7.0, 9.1 and 4.8 kg/ha.mm rainfall for rehabilitated sites and unmined sites on Brigalow Uplands, Mountain Coolibah and Poplar Box land types respectively. Long-term carrying capacities based on estimates of long-term median pasture growth and 30% utilisation were 4.39, 3.58 and 5.92 ha/adult equivalent respectively for the unmined land types, and 2.45 ha/adult equivalent for the rehabilitated lands. Conclusions: Rehabilitated land can be as productive as unmined but previously cultivated land. Implications: Grazing management plans for sustainable management of mined and unmined lands can be developed using data from the present study. The plans will assist with the transition of rehabilitated lands to commercial agriculture

Sustainability of beef production from brigalow lands after cultivation and mining. 3. Pasture rundown, climate and grazing pressure effects

Context: The Acland Land System overlying the Walloon sandstone coal deposits in southern Queensland is generally marginal for cropping but well suited to grazing, and thus cultivated land is commonly returned to pasture. Rehabilitation of these lands after open-cut coal mining seeks to be safe, stable and self-sustaining to satisfy requirements for ecologically sustainable development. Aims: The present paper evaluates the sustainability and economic viability of beef production on (a) lands retired from cultivation and then rehabilitated with sown pastures after open-cut coal mining at the New Acland mine site, and (b) similar nearby pasture lands that were not mined but were also retired from cultivation. Methods: The GRASP grazing systems model was modified and calibrated with short-term (5-year) grazing trial data (soil, pasture and cattle observations), and then used with long-term (60-year) weather data to estimate effects of land type, pasture rundown, climate and grazing pressure on productivity and economic returns. The productivity of three rehabilitated sites and 15 unmined sites were evaluated, including pastures on six commercial properties. Key results: Estimates of long-term mean annual growth of pastures on unmined lands retired from cultivation on three land types (Mountain Coolibah, Brigalow Uplands and Poplar Box) were 3398, 2817 and 2325 kg/ha respectively. Pasture growth was greater on rehabilitated lands; 3736 kg/ha on the site most typical of rehabilitated lands and a mean of 4959 kg/ha across three sites. Seasonal conditions had large effects on cattle liveweight gain (133–213 kg/head per year during the trial); however, pasture growth was the main driver of beef production and economic returns per hectare. In GRASP, potential nitrogen uptake was used to influence key pasture growth processes and accounted for 64% of variation in observed annual growth. The short-term lift and subsequent rundown in productivity typically associated with sown pastures was estimated to have increased mean annual pasture and cattle productivity during the 2014–2018 trial period by up to 17% and 25% respectively. Estimates of long-term mean annual beef production and economic returns for the unmined lands were less than estimated for rehabilitated lands and were 139 kg/head.year (45 kg/ha.year) and AU$154/adult equivalent. Conclusions: Rehabilitated lands were found to be sustainable for beef production at grazing pressures up to 30% utilisation of annual pasture growth, and comparable with grazing systems on native and sown pastures in good condition. Pastures on unmined lands retired from cultivation had reduced productivity. Implications: Overgrazing is a significant and on-going residual risk to sustainable production. Grazing regimes need to continually adjust for changes in novel landscapes, pasture condition and climate. The methods used in the present study could be applied more generally

Forgotten Plotlanders: Learning from the survival of lost informal housing in the UK.

Colin Ward’s discourses on the arcadian landscape of ‘plotlander’ housing are unique documentations of the anarchistic birth, life, and death of the last informal housing communities in the UK. Today the forgotten history of ‘plotlander’ housing documented by Ward can be re-read in the context of both the apparently never-ending ‘housing crisis’ in the UK, and the increasing awareness of the potential value of learning from comparable informal housing from the Global South. This papers observations of a previously unknown and forgotten plotlander site offers a chance to begin a new conversation regarding the positive potential of informal and alternative housing models in the UK and wider Westernised world

HIV Drug Resistance Mutations in Non-B Subtypes After Prolonged Virological Failure on NNRTI-Based First-Line Regimens in Sub-Saharan Africa

OBJECTIVE: To determine drug resistance mutation (DRM) patterns in a large cohort of patients failing nonnucleoside reverse transcriptase inhibitor (NNRTI)-based first-line antiretroviral therapy regimens in programs without routine viral load (VL) monitoring and to examine intersubtype differences in DRMs. DESIGN: Sequences from 787 adults/adolescents who failed an NNRTI-based first-line regimen in 13 clinics in Uganda, Kenya, Zimbabwe, and Malawi were analyzed. Multivariable logistic regression was used to determine the association between specific DRMs and Stanford intermediate-/high-level resistance and factors including REGA subtype, first-line antiretroviral therapy drugs, CD4, and VL at failure. RESULTS: The median first-line treatment duration was 4 years (interquartile range 30-43 months); 42% of participants had VL ≥100,000 copies/mL and 63% participants had CD4 <100 cells/mm. Viral subtype distribution was A1 (40%; Uganda and Kenya), C (31%; Zimbabwe and Malawi), and D (25%; Uganda and Kenya), and recombinant/unclassified (5%). In general, DRMs were more common in subtype-C than in subtype-A and/or subtype-D (nucleoside reverse transcriptase inhibitor mutations K65R and Q151M; NNRTI mutations E138A, V106M, Y181C, K101E, and H221Y). The presence of tenofovir resistance was similar between subtypes [P (adjusted) = 0.32], but resistance to zidovudine, abacavir, etravirine, or rilpivirine was more common in subtype-C than in subtype-D/subtype-A [P (adjusted) < 0.02]. CONCLUSIONS: Non-B subtypes differ in DRMs at first-line failure, which impacts on residual nucleoside reverse transcriptase inhibitor and NNRTI susceptibility. In particular, higher rates of etravirine and rilpivirine resistance in subtype-C may limit their potential utility in salvage regimens

Oldest pathology in a tetrapod bone illuminates the origin of terrestrial vertebrates

The origin of terrestrial tetrapods was a key event in vertebrate evolution, yet how and when it occurred remains obscure, due to scarce fossil evidence. Here, we show that the study of palaeopathologies, such as broken and healed bones, can help elucidate poorly understood behavioural transitions such as this. Using high-resolution finite element analysis, we demonstrate that the oldest known broken tetrapod bone, a radius of the primitive stem tetrapod Ossinodus pueri from the mid-Viséan (333 million years ago) of Australia, fractured under a high-force, impact-type loading scenario. The nature of the fracture suggests that it most plausibly occurred during a fall on land. Augmenting this are new osteological observations, including a preferred directionality to the trabecular architecture of cancellous bone. Together, these results suggest that Ossinodus, one of the first large (&gt;2m length) tetrapods, spent a significant proportion of its life on land. Our findings have important implications for understanding the temporal, biogeographical and physiological contexts under which terrestriality in vertebrates evolved. They push the date for the origin of terrestrial tetrapods further back into the Carboniferous by at least two million years. Moreover, they raise the possibility that terrestriality in vertebrates first evolved in large tetrapods in Gondwana rather than in small European forms, warranting a re-evaluation of this important evolutionary event

Erratum: Corrigendum: Sequence and comparative analysis of the chicken genome provide unique perspectives on vertebrate evolution

International Chicken Genome Sequencing Consortium. The Original Article was published on 09 December 2004. Nature432, 695–716 (2004). In Table 5 of this Article, the last four values listed in the ‘Copy number’ column were incorrect. These should be: LTR elements, 30,000; DNA transposons, 20,000; simple repeats, 140,000; and satellites, 4,000. These errors do not affect any of the conclusions in our paper. Additional information. The online version of the original article can be found at 10.1038/nature0315